Complexo enzimático na dieta de alevinos de kinguio (Carassius auratus)

Aiming to evaluate the enzymatic complex supplementation in diets for goldfish fingerlings (Carassius auratus), 240 fish weighing initially 1,36 ± 0,02g, randomly distributed in 20 tanks with 150L, in four treatments and five replications, with twelve fish in each experimental unit were used. The fish were fed at 8:00 and 11:00 a.m. and 2:00 and 5:00 p.m. with diets containing different inclusion levels (0; 0,033; 0,066 e 0,099%) of enzymatic complex (amilase, protease, celulase, lipase, â-glucanase and phytase), and formulated with 32,36% of digestible protein and 3.023kcal of digestible energy kg-1. There were no differences observed (P>0,05) in the mean final weight, weight gain, total length, standard length, survival and carcass composition. However, the fish apparent feed conversion was impaired by the supplementation of enzymatic complex with 0,099% in diet. The use of enzymatic complex does not provides benefits in the productive performance for goldfish fingerlings.

Desempeño de tanques decantadores de sólidos en un sistema de recirculación para producción de tilapia

To compare the removal efficiency of solids, turbidity and apparent color between a conventional and a column settling tanks in a recirculating aquaculture system (RAS) for tilapia farming. Materials and methods. Tilapia with a stocking density between 30 and 33 kg/m3 were cultured in a RAS consisting of a water level control box, PVC piping system, three plastic tanks for culture, conventional horizontal flow settling tank (Con.ST), column vertical flow settling tank (Col.ST), three phase fluidized bed reactor, oxygen transfer reactor, air compressor, air blower, centrifugal pump. The Con.ST operated at a volume of 1.4 m3 and hydraulic retention time (HRT) of 2.94 h; and was drained weekly for washing and sludge collection, representing a 55%discharge of system water volume. The Col.ST operated with a volume of 0.30 m3 and HRT of 0.553 h. Three daily partial draining operations were executed, representing a discharge of 50% of the system volume. Results. The mean solids removal efficiencies were: 34.01 and 44.44%for total solids; 64.45 and 71.71% for suspended solids; 21.10 and 45.65% volatile solids; 65.51% and 62.79% for turbidity; and 56.37 and 50.91% for apparent color, respectively for Con.ST and Col.ST. Conclusions. The two settling devices are useful on removal of the studied parameters and presented similar performance on turbidity and apparent color removal; however, the Col.ST was more efficient than Con.ST for solids removal, requires less space, less volume and requires less discharge water volume, displaying feasibility for its use on RAS.

Differential arthritogenicity of Staphylococcus aureus strains isolated from biological samples

Background: Staphylococcus aureus is the most common agent of septic arthritis that is a severe, rapidly progressive and destructive joint disease. Superantigens produced by S. aureus are considered the major arthritogenic factors. In this study, we compared the arthritogenic potential of five superantigen-producing staphylococcal strains.Methods: Male C57BL/6 mice were intravenously infected with ATCC 19095 SEC+, N315 ST5 TSST-1+, S-70 TSST-1+, ATCC 51650 TSST-1+ and ATCC 13565 SEA+ strains. Clinical parameters as body weight, arthritis incidence and clinical score were daily evaluated. Joint histopathological analysis and spleen cytokine production were evaluated at the 14th day after infection.Results: Weight loss was observed in all infected mice. ATCC 19095 SEC+, N315 ST5 TSST-1+ and S-70 TSST-1+ were arthritogenic, being the highest scores observed in ATCC 19095 SEC+ infected mice. Intermediate and lower clinical scores were observed in N315 ST5 TSST-1+ and S-70 TSST-1+ infected mice, respectively. The ATCC 13565 SEA+ strain caused death of 85% of the animals after 48 h. Arthritis triggered by the ATCC 19095 SEC+ strain was characterized by accentuated synovial hyperplasia, inflammation, pannus formation, cartilage destruction and bone erosion. Similar joint alterations were found in N315 ST5 TSST-1+ infected mice, however they were strikingly more discrete. Only minor synovial proliferation and inflammation were triggered by the S-70 TSST-1+ strain. The lowest levels of TNF-α, IL-6 and IL-17 production in response to S. aureus stimulation were found in cultures from mice infected with the less arthritogenic strains (S-70 TSST-1+ and ATCC 51650 TSST-1+). The highest production of IL-17 was detected in mice infected with the most arthritogenic strains (ATCC 19095 SEC+ and N315 ST5 TSST-1+).Conclusions: Together these results demonstrated that S. aureus strains, isolated from biological samples, were able to induce a typical septic arthritis in mice. These results also suggest that the variable arthritogenicity of these strains was, at least in part, related to their differential ability to induce IL-17 production. © 2013 Colavite-Machado et al.; licensee BioMed Central Ltd.

Effects of a homeopathic complex on the performance and cortisol levels in Nile tilapia (Oreochromis niloticus)

Background: Intensive fish farming results in stress adversely effecting the performance of farmed fish. Plasma cortisol is a validated measure of stress in fish. We evaluated the effect of a homeopathic complex on the cortisol level of Nile tilapias (Oreochromis niloticus). Method: 60 animals with approximate average weight of 100 g each at the start of experiment were randomly distributed in six glass fiber water tanks, capacity 1000 liters, with a daily water renewal rate of 20%. They received one of two treatments: 30 animals in control treatment and 30 animals receiving the homeopathic complex Homeopatila 100. On days 1, 30 and 60, all fish were anesthetized and blood was collected by puncture on the caudal vein, to determine the levels of circulating cortisol. Results: At the end of the experiment the fish receiving a homeopathic complex, had significantly lower circulating cortisol level (17.96 ng/mL ± 0.95) than the control group (38.68 ng/mL ± 1.21) (p < 0.05). Conclusions: Cortisol levels were significantly lower in the treated group than control, and the fish were larger in the treated group. © 2013 The Faculty of Homeopathy.

Frequência de arraçoamento para alevinos de carpa comum

The best frequency of feeding for development of common carp fingerlings Cyprinus carpio was determined. Four hundred fish (4.88 ± 1.41 g and 6.72 ± 0.70 cm) were used, distributed in 20 tanks of 250 L each in completely randomized design with four treatments and five replicates. The four feeding strategies adopted were: providing feed once (T1), twice (T2), three (T3) and four times daily (T4) at the same amount being fractionated as described. At the end of 45 days of experiment were carried out measurements of the final weight (FW), final length (FL), weight gain (WG), feed conversion (FC), specific growth rate (SGR) and survivor (SR). It was evaluated the chemical composition of fish carcass: moisture (MT), crude protein (CP), lipids (LP) and ash (AS) and hematological analyzes performed such as erythrocyte (ER), hemoglobin (HG) and hematocrit (HT). There were significant statistical differences (P<0.01) for FW, FL, WG and SGR. For FC and SR there were no statistical differences (P>0.01). For the chemical composition the moisture, lipids and ash showed significant statistical differences (P>0.01), except only for protein (P>0.01) The hematological composition was not influenced by the feed frequency, it was not observed significant statistical difference (P>0.01) of erythrocytes, hemoglobin and hematocrit among dietary treatments. For common carp fingerlings, the provision of ration four times daily provided better final weight, final length, weight gain and specific growth rate without causing changes in the composition and hematological parameters of the fish.

Eficácia de fluridone para o controle de Egeria spp. em caixas d'água e em represa de pequeno porte sem fluxo de água

Pós-graduação em Agronomia (Agricultura) - FCA

Análise da produtividade e viabilidade econômica de quatro sistemas de berçários do camarão-da-Amazônia, Macrobrachium amazonicum

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Probiótico na alimentação do pacu (Piaractus mesopotamicus): avaliação hematológica, bioquímica, imunológica e desempenho produtivo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Viabilidade técnica e econômica da larvicultura do camarão-da-amazônia, Macrobrachium amazonicum, em diferentes temperaturas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação da qualidade de efluentes na criação de rã-touro (Lithobates catesbeianus)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação do desempenho de tilápias (Oreochromis niloticus, Trewavas, 1983) em tanques-rede na represa de Jurumirim/Alto Rio Paranapanema

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Níveis de arraçoamento e freqüência alimentar no desempenho produtivo do acará-bandeira Pterophyllum scalare

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Alterações hematológicas e hormonais em fêmeas de jundiá (Rhamdia quelen) provocadas por estresse mecânico e contaminação da água por herbicida a base de glifosato

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Uso da Aloe vera L. no manejo de matrinxã (Brycon amazonicus)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Parâmetros produtivos, fisiológicos e imunológicos de juvenis de pacu (Piaractus mesopotamicus) submetidos à restrição alimentar e estresse de manejo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)