440 resultados para Potencial enológico de clones
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The metabolic effects caused by hydric deficiency (HD) on Eucalyptus grandis clones were assessed by an experiment where plants were cultivated in four blocks. The first was the control block, normally irrigated, whereas the other three blocks were submitted to cycles of hydric deficiency. Analysis of photosynthetic efficiency, enzymatic activity of antioxidant response system, level of pigments and L-proline concentration were performed to evaluate the HD effects. Results showed that HD altered some parameters related to photosynthetic activity, pigments accumulation, proline and enzymatic activity. Clone 433 of E. grandis presented higher response ability to HD.
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Considerable losses during apple fruit storage occur due to microbiological diseases, mainly caused by Penicillium expansum, which in addition to fruit pulp deterioration produces patulin, a mycotoxin with carcinogenic and teratogenic activity. Biological control of post-harvest disease by antagonist yeasts focused on killer toxins is an appreciable alternative to the chemical fungicides, due to the low possibility of toxic residues demonstrated during fermentative processes. Twenty out of 44 yeasts (16 isolated from fruits, 10 from corn silage and 18 from laboratory anthill), showed antagonism against spores of P. expansum. The assay in solid medium pointed the strongest nutrient competition antagonism by D. hansenii strain C1 (31 mm inhibition diameter), while D. hansenii strain C7 (15 mm) showed higher antibiosis and parasitism pattern. In the following step the extracellular activity was tested performing the assay with culture supernatant in Yeast Medium agar, where C. guilliermondii P3 was more effective against conidia germination (inhibition rate of 58.15%) while P. ohmeri showed better inhibition on micelial growth (66.17%). The antibiosis showed by both yeasts could suggest probable mechanism associated with killer phenomenon, once both strains were killer positive against sensitive reference strains (S. cerevisiae NCYC 1006 and P. kluyveri CAY-15). In order to enhance the production of antifungal substance, these yeasts were cultivated with P. expansum, but the difference between culture supernatant obtained from yeasts cultivated alone and with mould was not significant (P > 0.05). The results demonstrated that the yeasts application constitute a promising tool, enhancing the biological control of P. expansum in post-harvest diseases of apple fruit.
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Bats are hosts of a rich diversity of microorganisms. Many studies indicate a close link between bats and fungi with pathogenic potential, especially for living in environments such as caves, caverns and hollow trees, favorable to the maintenance and spread of fungi. The objective was to study the gastrointestinal mycoflora of bats. Of the 98 samples belonging to 11 species of bats coming from 15 studied cities, 20% of the species were Carollia perspicillata, 19% Artibeus lituratus, 17% Molossus rufus, 13% Glossophaga soricina, 9% Nyctinomops macrotis, 8% Molossus molossus, 7% Desmodus rotundus, 2% Lasiurus ega and 1% Eptesicus furinalis, Myotis nigricans and Tadarida brasiliensis. The genus Aspergillus sp. was isolated from 29% of the samples, followed by 6% Microsporum sp. and Penicillium sp. 4% Trichophyton sp. and zygomycetes and 2% Fusarium sp. Of yeast species, 14% were from Rhodotorula sp., 10% Candida sp. and 2% Cryptococcus sp., 22% of isolates remained unidentified. All 82 cultures of organs were negative for Histoplasma capsulatum. There was a statistically significant association between the results of microbiological culture and bat species (p < 0.05). We conclude that the bats can act as disperser agents of fungi with pathogenic potential, although other studies should be performed to establish strategies to identify the main factors correlated with the growth and spread of microorganisms in nature and implication of bats in the epidemiological cycle.
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The drift is intimately linked to inappropriate use of pesticides and an important factor for reducing it, is the correct selection of spray nozzles and adjuvants. The objective of this work was to evaluate the drift potential in wind tunnel with different spray nozzles and different concentrations of adjuvants. The experiment was composed by six spray solutions (vegetable oil (in three concentrations), mineral oil, surfactant and reducing drift), which were applied with two nozzles, one pre-orifice flat fan (DG 8003 VS) and other with air induction (AI 8003 VS), totaling 12 treatments, with three repetitions. The equipment used was a wind tunnel, where the drift collections were made at different points. The treatments averages were compared using Confidence Interval at 5% probability. The analysis of the percentage of drift showed that the treatments had different behaviors. The nonylphenol ethoxylate adjuvant presented the highest drift when applied with the nozzle of pre-orifice and the lowest drift when applied with the air induction. The behavior of these nozzles when the oil-based adjuvant was used showed apposite results to those obtained for the surfactant. For the DG nozzles the lowest percentage of drift, at all analyzed distances, was observed to the treatment with vegetable oil (1.0%) and with the AI nozzles the lower drift was found for the treatment with nonylphenol ethoxylate (0.0625%), for the four distances in the collection. The result showed that both the spray nozzles and adjuvants alter directly the drift potential. There was no proportionately between the concentration of the oil-based adjuvant and the drift percentage.
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Our aim was to investigate the population fluctuation and the damage caused by the phytophagous mites Calacarus heveae Feres, Tenuipalpus heveae Baker, and Eutetranychus banksi (McGregor) on clones FX 2784, FX 3864, and MDF 180 in rubber tree crops from southeastern Bahia, Brazil. Moreover, we tested for the influence of climatic variables on occurrence patterns of these species throughout weekly samples performed from October to April. The infestation peaks was between mid-January and late February. The clones FX 2784 and FX 3864 had the highest infestations and more severe damage possibly caused by C. heveae, which was the most frequent and abundant species in all clones. We found that sunlight duration and rainfall were the most important factors for C. heveae while T. heveae was affected by rainfall and temperature. Eutetranychus banksi was only affected by sunlight duration. However, the best models had low goodness of fit. We concluded that the clones FX 2784 and FX 3864 had a higher susceptibility to mite attack, and the association between climatic variables and favorable physiological conditions were determinant for the population increase of the species from January to April. © 2012 Sociedade Entomológica do Brasil.
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Common bean is an important crop in Brazil primarily because of its nutritional characteristics. Some agronomic practices, such as weed management, are fundamental to cultivation, as a means of obtaining a high crop yield. However, some studies have shown that weed management may alter the function of the cultivar cycle. Thus, this study aimed at determining the optimal phenological stage in early-maturing common bean cultivars to perform the weed control without providing reductions in yield and seed quality. The experimental design was randomized blocks with 20 treatments and four replications, in a 2×2×5 (cultivars × types of weed control × periods of weed control) factorial scheme. The periods of weed control for both cultivars (Carioca Precoce and IPR-Colibri) consisted of full cycle weeded (control), weed control at the V4-3 stage (first three nodes on the main stem with trifoliate leaves), at the R5 stage (beginning of bloom) and at the R8 stage (appearance of pods) and full cycle unweeded (no weed control). The types of weed control used were chemical (fluazifop-p-butyl + fomesafen) and mechanical (hoe). The Carioca Precoce cultivar demonstrated higher agronomic performance and yield than the IPR-Colibri cultivar, although the IPR-Colibri seeds had a higher vigor. The type of weed control (chemical or mechanical) did not affect the agronomic characteristics, yield and seed physiological potential of the cultivars. The ideal period for weed control in early-maturing common bean cultivars to obtain a higher yield and seed physiological potential was observed at the V4-3 phenological stage.
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In this study the effects of the time of male permanence with females and different sex ratios on the reproductive capacity of Chrysoperla externa F 3 adults was evaluated. The insects were kept at 25 ± 2 °C, 70 ± 10% RH, under a 12 h photoperiod. The first experiment consisted of mating females with males that were subsequently removed after 5, 10, 15, 20, 25, 30, 35 and 40 days; the control group consisted of males remaining with the females during the entire experimental period. The second experiment consisted of mating: one male with one female (1:1); one male with two females (1:2); one male with three females (1:3); one male with four females (1:4); and one male with five females (1:5), and then evaluating their pre-ovipositioning periods, daily and total ovipositioning, egg viability percentages, and efficiency indices (which considered total ovipositioning, mean egg viability, and the ovipositioning period). It was found that alternating the sexual proportions from one male with one female to one male with three females did not alter the reproductive performance of these insects, and that there was no need to replace the males after five days as they were able to provide sufficient numbers of spermatozoa during this period to maintain consistent reproductive rates.
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The rubber tree [Hevea brasiliensis (Willd. ex Adr. Of Juss.) Muell. Arg.] can be affected by the occurrence of the fungus Oidium heveae, which causes one of the most important diseases of rubber trees, powdery mildew. This work meet changes in photosynthetic pigments, an indicator of oxidative stress, in seedlings of three Hevea brasiliensis clones, RRIM 600, GT1 and PR255, under infection in Oidium heveae. The experiment was conducted in an open environment under natural photoperiod conditions and at the beginning of the trial, the rubber plants that would be inoculated were sprayed with an aqueous suspension containing Oidium heveae at a concentration of 16 x 104conidia mL-1. On the day of inoculation and after 48, 96, 144 and 192 h leaf samples were collected for the determination of photosynthetic pigments. Degradation in photosynthetic pigments in the period of infection was observed in rubber tree clones studied; thus, there is oxidative stress in clones of rubber trees. No promising genetic material for genetic improvement work stress tolerance by Oidium heveae was identified.
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Oxacillin is an alternative for the treatment of Staphylococcus spp. infections; however, resistance to this drug has become a major problem over recent decades. The main objective of this study was to epidemiologically characterize coagulase-negative staphylococci (CoNS) strains recovered from blood of patients hospitalized in a Brazilian teaching hospital. Oxacillin resistance was analyzed in 160 strains isolated from blood culture samples by phenotypic methods, detection of the mecA gene, and determination of intermediate sensitivity to vancomycin on brain heart infusion agar supplemented with 4 and 6 μg/mL vancomycin. In addition, characterization of the epidemiological profile by staphylococcal cassette chromosome mec (SCC. mec) typing and clonal analysis by pulsed-field gel electrophoresis (PFGE) were performed. The mecA gene was detected in 72.5% of the isolates. Methicillin-resistant CoNS isolates exhibited the highest minimum inhibitory concentrations and multiresistance when compared to methicillin-susceptible CoNS strains. Typing classified 32.8% of the isolates as SCC. mec I and 50% as SCC. mec III. PFGE typing of the SCC. mec III Staphylococcus epidermidis isolates identified 6 clones disseminated in different wards that persisted from 2002 to 2009. The high oxacillin resistance rates found in this study and clonal dissemination in different wards highlight the importance of good practices in nosocomial infection control and of the rational use of antibiotic therapy in order to prevent the dissemination of these clones. © 2013 Elsevier Inc.
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Pós-graduação em Bases Gerais da Cirurgia - FMB
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Aquicultura - FCAV
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Ciências Cartográficas - FCT