Expression of a new Bacillus thuringiensis Cry1Ia gene in Escherichia coli with strong activity against cotton pests

The control of cotton pests may be accomplished using Bacillus thuringiensis Cry proteins. For this purpose, the objective of this work was to evaluate the insecticidal activity of a new Cry1Ia protein against neonatal larvae of Spodoptera frugiperda and Anthonomus grandis. The complete cry1Ia gene, previously obtained by PCR with oligonucleotide primers based on the sequenced gene, was cloned into the vector pET28a(+), introduced into Escherichia coli BL21(DE3) and expressed by induction with IPTG. The expression of the Cry1Ia protein was confirmed with molecular weight of approximately 81 kDa. The results demonstrated the efficiency of the bacterial system for the expression of B. thuringiensis Cry1Ia protein, which was subsequently used in quantitative bioassays against S. frugiperda and A. grandis larvae, resulting in an extremely toxic protein for both species. This characteristic is exceptionally important for obtaining transgenic cotton plants resistant to these pests.

New trends in musical genre classification using optimum-path forest

Musical genre classification has been paramount in the last years, mainly in large multimedia datasets, in which new songs and genres can be added at every moment by anyone. In this context, we have seen the growing of musical recommendation systems, which can improve the benefits for several applications, such as social networks and collective musical libraries. In this work, we have introduced a recent machine learning technique named Optimum-Path Forest (OPF) for musical genre classification, which has been demonstrated to be similar to the state-of-the-art pattern recognition techniques, but much faster for some applications. Experiments in two public datasets were conducted against Support Vector Machines and a Bayesian classifier to show the validity of our work. In addition, we have executed an experiment using very recent hybrid feature selection techniques based on OPF to speed up feature extraction process. © 2011 International Society for Music Information Retrieval.

PAR-COM: A new methodology for post-processing association rules

The post-processing of association rules is a difficult task, since a huge number of rules that are generated are of no interest to the user. To overcome this problem many approaches have been developed, such as objective measures and clustering. However, objective measures don't reduce nor organize the collection of rules, therefore making the understanding of the domain difficult. On the other hand, clustering doesn't reduce the exploration space nor direct the user to find interesting knowledge, therefore making the search for relevant knowledge not so easy. In this context this paper presents the PAR-COM methodology that, by combining clustering and objective measures, reduces the association rule exploration space directing the user to what is potentially interesting. An experimental study demonstrates the potential of PAR-COM to minimize the user's effort during the post-processing process. © 2012 Springer-Verlag.

Characterization of texture in image of skin lesions by support vector machine

Due to the increased incidence of skin cancer, computational methods based on intelligent approaches have been developed to aid dermatologists in the diagnosis of skin lesions. This paper proposes a method to classify texture in images, since it is an important feature for the successfully identification of skin lesions. For this is defined a feature vector, with the fractal dimension of images through the box-counting method (BCM), which is used with a SVM to classify the texture of the lesions in to non-irregular or irregular. With the proposed solution, we could obtain an accuracy of 72.84%. © 2012 AISTI.

Development of a support vector machine-based navigation system for driving a mobile robot through paths in plantations

The use of mobile robots turns out to be interesting in activities where the action of human specialist is difficult or dangerous. Mobile robots are often used for the exploration in areas of difficult access, such as rescue operations and space missions, to avoid human experts exposition to risky situations. Mobile robots are also used in agriculture for planting tasks as well as for keeping the application of pesticides within minimal amounts to mitigate environmental pollution. In this paper we present the development of a system to control the navigation of an autonomous mobile robot through tracks in plantations. Track images are used to control robot direction by preprocessing them to extract image features. Such features are then submitted to a support vector machine in order to find out the most appropriate route. The overall goal of the project to which this work is connected is to develop a real time robot control system to be embedded into a hardware platform. In this paper we report the software implementation of a support vector machine, which so far presented around 93% accuracy in predicting the appropriate route. © 2012 IEEE.

Purification, characterization, and specificity determination of a new serine protease secreted by penicillium waksmanii

The purpose of this work was to purify a protease from Penicillium waksmanii and to determine its biochemical characteristics and specificity. The extracellular protease isolated that was produced by P. waksmanii is a serine protease that is essential for the reproduction and growth of the fungus. The protease isolated showed 32 kDa, and has optimal activity at pH 8.0 and 35 C towards the substrate Abz-KLRSSKQ-EDDnp. The protease is active in the presence of CaCl2, KCl, and BaCl, and partially inhibited by CuCl2, CoCl2 and totally inhibited by AlCl3 and LiCl. In the presence of 1 M urea, the protease remains 50 % active. The activity of the protease increases 60 % when it is exposed to 0.4 % nonionic surfactant-Triton X-100 and loses 10 % activity in the presence of 0.4 % Tween-80. Using fluorescence resonance energy transfer analysis, the protease showed the most specificity for the peptide Abz-KIRSSKQ-EDDnp with k cat/K m of 10,666 mM-1 s-1, followed by the peptide Abz-GLRSSKQ-EDDnp with a k cat/K m of 7,500 mM -1 s-1. Basic and acidic side chain-containing amino acids performed best at subsite S1. Subsites S2, S3, S′ 2, and S′ 1, S ′ 3 showed a preference for binding for amino acids with hydrophobic and basic amino acid side chain, respectively. High values of k cat/K m were observed for the subsites S2, S3, and S′ 2. The sequence of the N-terminus (ANVVQSNVPSWGLARLSSKKTGTTDYTYD) showed high similarity to the fungi Penicillium citrinum and Penicillium chrysogenum, with 89 % of identity at the amino acid level. © 2012 Springer Science+Business Media New York.

Search for new physics in events with opposite-sign leptons, jets, and missing transverse energy in pp collisions at s=7 TeV

A search is presented for physics beyond the standard model (BSM) in final states with a pair of opposite-sign isolated leptons accompanied by jets and missing transverse energy. The search uses LHC data recorded at a center-of-mass energy s=7 TeV with the CMS detector, corresponding to an integrated luminosity of approximately 5 fb-1. Two complementary search strategies are employed. The first probes models with a specific dilepton production mechanism that leads to a characteristic kinematic edge in the dilepton mass distribution. The second strategy probes models of dilepton production with heavy, colored objects that decay to final states including invisible particles, leading to very large hadronic activity and missing transverse energy. No evidence for an event yield in excess of the standard model expectations is found. Upper limits on the BSM contributions to the signal regions are deduced from the results, which are used to exclude a region of the parameter space of the constrained minimal supersymmetric extension of the standard model. Additional information related to detector efficiencies and response is provided to allow testing specific models of BSM physics not considered in this Letter. © 2012 CERN.

Spin squeezing and entanglement via finite-dimensional discrete phase-space description

We show how mapping techniques inherent to N2-dimensional discrete phase spaces can be used to treat a wide family of spin systems which exhibits squeezing and entanglement effects. This algebraic framework is then applied to the modified Lipkin-Meshkov-Glick (LMG) model in order to obtain the time evolution of certain special parameters related to the Robertson- Schrödinger (RS) uncertainty principle and some particular proposals of entanglement measure based on collective angular-momentum generators. Our results reinforce the connection between both the squeezing and entanglement effects, as well as allow to investigate the basic role of spin correlations through the discrete representatives of quasiprobability distribution functions. Entropy functionals are also discussed in this context. The main sequence correlations → entanglement → squeezing of quantum effects embraces a new set of insights and interpretations in this framework, which represents an effective gain for future researches in different spin systems. © 2013 World Scientific Publishing Company.

Closed poly-trajectories and Poincaré index of non-smooth vector fields on the plane

This paper is concerned with closed orbits of non-smooth vector fields on the plane. For a class of non-smooth vector fields we provide necessary and sufficient conditions for the existence of closed poly-trajectorie. By means of a regularization process we prove that hyperbolic closed poly-trajectories are limit sets of a sequence of limit cycles of smooth vector fields. In our approach the Poincaré Index for non-smooth vector fields is introduced. © 2013 Springer Science+Business Media New York.

Observation of a new boson with mass near 125 GeV in pp collisions at √=7 and 8 TeV

A detailed description is reported of the analysis used by the CMS Collaboration in the search for the standard model Higgs boson in pp collisions at the LHC, which led to the observation of a new boson. The data sample corresponds to integrated luminosities up to 5.1 fb-1 at √=7 TeV, and up to 5.3 fb-1 at √ s=8 TeV. The results for five Higgs boson decay modes γγ, ZZ, WW, ττ, and bb, which show a combined local significance of 5 standard deviations near 125 GeV, are reviewed. A fit to the invariant mass of the two high resolution channels, γγ and ZZ → 4ℓ, gives a mass estimate of 125.3 ± 0.4 (stat.) ± 0.5 (syst.) GeV. The measurements are interpreted in the context of the standard model Lagrangian for the scalar Higgs field interacting with fermions and vector bosons. The measured values of the corresponding couplings are compared to the standard model predictions. The hypothesis of custodial symmetry is tested through the measurement of the ratio of the couplings to the W and Z bosons. All the results are consistent, within their uncertainties, with the expectations for a standard model Higgs boson. [Figure not available: see fulltext.] © 2013 CERN for the benefit of the CMS collaboration.

The Genome of Anopheles darlingi, the main neotropical malaria vector

Anopheles darlingi is the principal neotropical malaria vector, responsible for more than a million cases of malaria per year on the American continent. Anopheles darlingi diverged from the African and Asian malaria vectors ∼100 million years ago (mya) and successfully adapted to the New World environment. Here we present an annotated reference A. darlingi genome, sequenced from a wild population of males and females collected in the Brazilian Amazon. A total of 10 481 predicted protein-coding genes were annotated, 72% of which have their closest counterpart in Anopheles gambiae and 21% have highest similarity with other mosquito species. In spite of a long period of divergent evolution, conserved gene synteny was observed between A. darlingi and A. gambiae. More than 10 million single nucleotide polymorphisms and short indels with potential use as genetic markers were identified. Transposable elements correspond to 2.3% of the A. darlingi genome. Genes associated with hematophagy, immunity and insecticide resistance, directly involved in vectorhuman and vectorparasite interactions, were identified and discussed. This study represents the first effort to sequence the genome of a neotropical malaria vector, and opens a new window through which we can contemplate the evolutionary history of anopheline mosquitoes. It also provides valuable information that may lead to novel strategies to reduce malaria transmission on the South American continent. The A. darlingi genome is accessible at www.labinfo.lncc.br/index.php/anopheles- darlingi. © 2013 The Author(s).

HTS: A new shape descriptor based on Hough Transform

In this paper we propose a novel method for shape analysis called HTS (Hough Transform Statistics), which uses statistics from Hough Transform space in order to characterize the shape of objects in digital images. Experimental results showed that the HTS descriptor is robust and presents better accuracy than some traditional shape description methods. Furthermore, HTS algorithm has linear complexity, which is an important requirement for content based image retrieval from large databases. © 2013 IEEE.

Histologic Characterization of Engineered Tissues in the Canal Space of Closed-apex Teeth with Apical Periodontitis

Introduction: The aim of this study was to investigate the capacity of endodontic regenerative procedures combining an induced blood clot, platelet-rich plasma (PRP), and bone marrow aspirate (BMA) to regenerate dental pulp in canine closed-apex necrotic teeth. Methods: Apical periodontitis was induced in 20 upper and lower premolars of 2 dogs. After biomechanical preparation, enlargement to a #60 file, and disinfection with a triantibiotic paste for 28 days, the roots were randomly assigned to 4 treatment groups: blood clot (BC), BC + PRP gel, BC + BMA gel, and BC + BMA/PRP gel. Negative controls were also included. After a 3-month follow-up period, the animals were killed. Results: Histologic analysis showed the presence of newly formed vital tissues (connective, cement-like, and bone-like tissue) in 23 of the 32 treated roots (71.87%). There was no statistically significant difference between the treatment groups. Conclusions: New vital tissues were formed and characterized as connective, cementum-like, or bone-like, but not as pulp-like tissue; PRP and/or BMA did not improve the tissue ingrowth. © 2013 American Association of Endodontists.

Controle robusto com realimentação derivativa de sistemas não lineares via LMI

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Dengue no Brasil: abordagem geográfica na escala nacional

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)