Description of a new species of Pseudopaludicola Miranda-Ribeiro, 1926 from the state of São Paulo, Southeastern Brazil (Anura, Leiuperidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Molecular characterization of Cryptosporidium spp. in dairy calves from the state of So Paulo, Brazil

Cryptosporidiosis is a common protozoan disease observed in a wide range of vertebrate hosts, including ruminants. Cattle can be a potential reservoir of Cryptosporidium spp., leading to environmental contamination with oocysts of zoonotic species. The molecular characterization of Cryptosporidium spp. isolated from cattle from the state of So Paulo, Brazil, was accomplished using nested polymerase chain reaction for amplification of fragments of the 18S rRNA gene and the glycoprotein GP60 gene, following sequencing of amplified fragments. Positivity for Cryptosporidium was found in 10.7% (21/196) of the samples. Four species of Cryptosporidium were identified: C. andersoni, C. bovis, C. parvum subtype IIaA15G2R1, and C. ryanae. To the best of our knowledge, this is the first report of infection by C. ryanae and C. parvum IIaA15G2R1 in cattle from Brazil.

FIRST RECORD of AN EPIBIONT PROTOZOAN EPISTYLIS SP (CILIOPHORA, PERITRICHIA) ATTACHED TO AMPLEXIBRANCHIUS BRYCONIS THATCHER & PAREDES, 1985 (COPEPODA, ERGASILIDAE) FROM PEIXE'S RIVER, STATE of São Paulo, BRAZIL

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Genetic population data of 12 STR loci of the PowerPlex (R) Y system in the state of São Paulo population (Southeast of Brazil)

Allele frequency distributions and population data for 12 Y-chromosomal short tandem repeats (STRs) included in the PowerPlex (R) Y Systems (Promega) were obtained for a sample of 200 healthy unrelated males living in S (a) over tildeo Paulo State (Southeast of Brazil). A total of 192 haplotypes were identified, of which 184 were unique and 8 were found in 2 individuals. The average gene diversity of the 12 Y-STR was 0.6746 and the haplotype diversity was 0.9996. Pairwise analysis confirmed that our population is more similar with the Italy, North Portugal and Spain, being more distant of the Japan. (c) 2007 Elsevier B.V. All rights reserved.

Abundance and temporal-spatial distribution of the rock shrimp Sicyonia dorsalis Kingsley, 1878 (Decapoda, Penaeoidea) from the northern coast of São Paulo state, Brazil.

Abundance and ecological distribution of the rock shrimp Sicyonia dorsalis from the northern coast of São Paulo State (Brazil) was investigated during a two-year period (July 2001 to June 2003). Monthly collections were conducted in the Ubatuba (UBA) and Caraguatatuba (CA) regions, using a commercial shrimp boat equipped with double-rig nets. In each region, seven depths were delimited (5 m to 35 m). A total of 1,752 and 1,894 shrimp were obtained at UBA and CA, respectively. The distribution of S. dorsalis differed among depths, years, and seasons. Highest abundance values were recorded in depths where silt and clay comprised more than 70% of the sediment. The majority (89%) of captured specimens were caught in the first year, in periods of lower mean temperature, mainly in the spring of 2001, when the intrusion of cold South Atlantic Central Waters (SACW) was more intense. In such period, the differences in the abundance of shrimps in each region (UBA = 1,118 and CA = 493) suggested that S. dorsalis migrated to the north (UBA), during the SACW intrusion. Thus, sediment type and water temperature are among the most important variables affecting the bathymetric distribution of S. dorsalis in these regions.

Characterization of Staphylococcus aureus isolates in milk and the milking environment from small-scale dairy farms of São Paulo, Brazil, using pulsed-field gel electrophoresis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Short Report: Vaccinia Virus in Humans and Cattle in Southwest Region of São Paulo State, Brazil

A new outbreak of Vaccinia virus was observed in Southwest region of São Paulo State. Brazil. The disease was observed in four small dairy farms with manual milking. Lesions were detected in cattle and in humans previously vaccinated and not vaccinated against smallpox. Although several reports of Vaccinia virus outbreaks have been occuring in Brazil, it was not yet reported in this region. This Outbreak reinforces the Viral circulation in Our country. The disease in persons previously vaccinated and not vaccinated against smallpox reinforces the absence of immunity, the risk to the human health, and the need for more epidemiologic and immunologic studies.

Molecular and parasitological survey of Hepatozoon canis (Apicomplexa : Hepatozoidae) in dogs from rural area of São Paulo state, Brazil

Hepatozoon canis is a protozoan that infects dogs and is transmitted by the ingestion of the brown dog tick, Rhipicephalus sanguineus. Two distinct species of Hepatozoon genus can infect dogs, H. canis and H. americanum. Routine tests to detect the disease are based on direct examination of gametocytes on Giemsa-stained blood smears. The objectives of this study were the investigation of infection prevalence in rural area dogs, the comparison of diagnostics by blood smear examination and polymerase chain reaction (PCR), and the association of infection with tick infestation. Blood smears, collected by puncture of the cephalic vein and ear margin capillary bed from 150 dogs, were examined. This technique detected 17 positive animals (11.3%), with 14 (9.3%) in peripheral blood and seven (4.7%) in cephalic vein blood. PCR tests detected 80 (53.3%) positive animals. R. sanguineus and Amblyomma spp. were found in 36 of the dogs (24%), in equal proportions. The identified species for Amblyomma genus were A. cajennense and A. ovale. Data analysis showed that PCR was much more sensitive when compared to blood smear examination. Hepatozoon species was previously identified as closely related to H. canis.

Homogeneity assessment of a station climate series (1933-2005) in the Metropolitan Area of São Paulo: instruments change and urbanization effects

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Fish, Tatui river basin, state of São Paulo, Brazil.

The Tatui river hydrographic basin locates in low Sorocaba river basin in São Paulo state, Brazil. A relatively high diversity of fish species was noticed at that place, whose habitats are also presumed to be diversified. The Tatui river is an important tributary of Sorocaba river, with great number of smaller streams and marginal lagoons. Fishes were collected using gill-nets, purse seines, sieves and traps, line and hook, every month during one year (from May 2009 to April 2010). Fish species were 51, including seven orders and 18 families. Characiformes and Siluriformes were the most represented with respectively 26 and 14 species.