158 resultados para Maturação ovariana
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Doppler ultrasonography is a new technology that has been study by researchers to improve the physiologic and pathologic knowledge about reproduction. This technology is based on Doppler-shifts frequencies or ultrasonic, these frequencies can be increase or decrease according to the movements of the red cells in the vessel. Color Doppler and power Doppler are the two possibilities to use the Doppler ultrasonography. Color Doppler is based in more the one color that show the direction of the blood f low and power Doppler is based in one color that change according of the flow intensity. Doppler ultrasonography can be demonstrated with the spectral mode to verify blood flow in large vessels, because of this, it is not use in equine reproduction. Studies in equine reproduction have been doing to verify uterus blood flow in cyclic mares and to observe the vascular perfusion in mares with cists, uterine vascular perfusion post breeding and verify the affects of drugs to decrease the uterus fluid in mares with problems in uterus perfusion. The ovarian irrigation during the estrus cycle was analyze with the measurement of the principal hormones during the estrus cycle in mares, the integrity of the corpus luteus, the irrigation of the future dominant follicle and the consequences in the ovarian irrigation after luteolyse induction also were study. Nevertheless, more than the knowledge that existed about Doppler ultrasonography, new studies have been doing to improve the forms to use Doppler ultrasonography in equine reproduction
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In the last years Brazil has achieved a leading position in worldwide cattle breeding. This country is consolidating the practice of an intensive and technical level cattle breeding, due to greater emphasis on reproductive efficiency and genetic improvement. The attention given to reproductive efficiency became to enhance the genetic improvement process, by exploiting the full reproductive potential of the animals. The biotechniques applied to reproduction arised to expand the reproductive potential for greater economic return. Are the most important reproductive biotechniques the FTAI and in vivo and in vitro embryo production for embryo transfer. Different hormonal treatments are consolidating to interfere with normal physiology in order to achieve the desired goals. The FTAI works to increase the reproductive rates of cattle through pre-booked artificial insemination in all the females in reproduction, without the need for estrous detection. The in vivo and in vitro embryo production aims to distribute the genetics of males and females genetically improved by transferring their embryos to genetically inferior females. Superovulation treatments designed to prevent the mechanism of dominance, which results in many follicles ovulating simultaneously for in vivo embryo production. To the in vitro embryo production, the technique of ovarian puncture guided by transvaginal ultrasound allows to aspirate a number of quality oocytes. The application of biotech is based on knowledge of the female and ovarian reproductive physiology and knowledge of their acting in different breeds and environments.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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O ambiente folicular é responsável por manter o oócito em parada meiótica e pela retomada da mesma posteriormente. A retomada da meiose em momento adequado é de extrema importância para o desenvolvimento de oócitos de alta competência. Portanto, o estudo dos mecanismos reguladores da retomada da meiose é de grande utilidade. Nesse estudo, os efeitos do FGF2, FGF10 e BMP15 sobre a progressão da meiose durante a maturação oocitária in vitro foram testados. Para tanto, complexos cumulus-oócitos (COCs) foram cultivados em meio definido seguindo o delineamento: Interação FGF10 e BMP15: sendo os tratamentos: controle; FGF10 (10ng/mL); BMP15 (100ng/mL); FGF10 (10ng/mL) + BMP15 (100ng/mL); e Dose-Resposta FGF2: sendo os tratamentos: controle e FGF2 nas doses 1, 10 ou 100ng/mL. A fase da meiose foi avaliada após 22h de cultivo por meio de coloração HOESCHT 33342, sendo os oócitos classificados em meiose I ou meiose II. O FGF10 (68%), a BMP15 (62,57%) ou a interação, FGF10+BMP15, (73%) não alteraram a porcentagem de oócitos em MII após a MIV em relação ao controle (69,63%). Assim como, a adição de FGF2 nas doses de 1ng/mL (81,33%), 10ng/mL (84,83%) ou 100ng/mL (83%) também não alteraram a porcentagem de oócitos em MII em relação ao controle (79,83%)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV
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Com o crescimento significativo da pecuária bovina brasileira houve o aumento de pesquisas e estudos que buscam animais cada vez mais precoces e produtivos. O presente trabalho é uma revisão de literatura com base em trabalhos que demonstram a eficiência da suplementação energética na maturação sexual de novilhas Nelore, visando a melhora da eficiência reprodutiva e antecipação da puberdade
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Zootecnia - FCAV
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This study was carried out to assess the influence of bovine embryo culture medium Beltsville Agriculture Research Center (BARC), supplemented with FCS, BSA or PVA, on the in vitro oocyte maturation, evidenced by cleavage rate and blastocysts production at different developmental stages. Three experiments were performed, as follows: exp.1: addition of FCS to BARC medium at concentrations of 0, 5 and 10%; exp. 2: addition of BSA to BARC medium at concentrations of 0, 4 and 8 mg/ml; exp. 3: addition of PVA to BARC medium at concentrations of 0, 0.5 and 1.0 mg/ml. TCM 199 supplemented with bicarbonate, pyruvate, gentamicin sulfate, FSH, LH and FCS was used as control group. Oocytes obtained from cow ovaries at slaughterhouse were selected in PBS, and then matured in BARC medium supplemented with FSH, LH and gentamicin sulfate, according to the experimental design. Percoll gradient was used for sperm selection and TALP medium for IVF. In vitro embryo culture was in SOF-m medium; a humidified atmosphere with 5% CO2, in air, at 38.7oC was used for all steps. The number of oocytes reaching blastocyst, expanded blastocyst, and hatched blastocyt stages was recorded, respectively at 72 and 168 h post-insemination. ANOVA and Bonferroni t test were used to determine differences among groups. Differences of P<0.05 were taken as significant. Higher percentage (P<0.05) of cleaved oocytes was observed in group TCM + FCS than for the other groups matured in BARC supplemented with FCS or BSA, regardless the concentration used. However, the cleavage rate was similar between groups BARC plus PVA with 1 mg/ml (85.7%) and TCM + FCS (90.8%). Significant difference was found among groups for the production of blastocysts, with the control group yielding a higher number of blastocysts (results ranging from 47.4 to 51.4%, in comparison with groups using BARC + FCS (4.1 to 19.7%), BSA (1.4 to 5.6%) and PVA (5.7 to 10.6%). In conclusion, BARC medium supplemented with different macromolecules did not promote a beneficial effect on in vitro oocyte maturation, resulting in lower rate of cleavage and blastocyst production when compared with TCM + FCS medium.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Caracterização física e química da carne de peito de aves de diferentes idades submetida à maturação
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)