Produção de vetores recombinantes para expressão de diferentes formas da proteína K1 do herpesvírus associado ao Sarcoma de Kaposi (KSHV)

Kaposi´s sarcoma associated herpesvirus (KSHV) or human herpesvirus 8 (HHV-8) is a gammaherpesvirus essential for the development of all forms of Kaposi´s sarcoma (KS). The KSHV’s life cycle is basically divided into latent and lytic phases, which have distinct viral gene expression profiles. Some important oncogenic products of KSHV are expressed during the lytic phase, including the viral K1 protein. As an effect of interfer-ence with intracellular signaling, K1 expression increases proliferation and survival of KSHV-infected cells. Due to its high level of genetic variability compared to other re-gions of the viral genome, the K1-encoding ORF (ORF-K1) is commonly evaluated for KSHV genotyping. It remains unclear whether different viral genotypes have particular biological effects that might modify the KSHV oncogenicity. The present study aimed to contribute to the establishment of an experimental in vitro model for evaluation of the K1 protein from common KSHV genotypes. Recombinant expression vectors with the ORF-K1 from KSHV genotypes A, B and C were prepared by genetic cloning. The recombi-nant vectors pKSHVOK1 obtained by cloning were sequenced for structural validation. After that, HEK293 cell line was transfected with the recombinant vectors, and proteins were extracted for expression analysis by Western blot technique, for K1 functional vali-dation. Results showed that ORF-K1 vectors containing KSHV ORF-K1 from the A, B and C genotypes were produced and structurally validated by DNA sequencing. The K1 expression at the protein level was also confirmed by immunoblots using an antibody for FLAG detection, an epitope from the vector that binds to K1. Based on presented re-sults, it´s possible to conclude that the recombinant vectors will be able to be used in future studies of K1 protein biological properties from distinct KSHV genotypes

Das formas de ensinar e conhecer o mundo: lições de coisas e método de ensino intuitivo na imprensa periódica educacional do século XIX

This article analyses A Eschola Publica, an educational journal that circulated in São Paulo state between 1893-1894 and 1896-1897, whose editors, allied with the diffusion of republican ideas, helped the consolidation of objective method as a pedagogical efficient modern tool. It was possible gathering some sources that shows the acceptance of that method and the difficulties for its dissemination in the classroom, mainly those refers to teacher’s formation. Taking as referential bibliographical resources about schooling culture it was possible verifying that circulating in complementary parallel way to textbooks, the articles by A Eschola Publica endorse the innovative purposes and show barriers imposing on educational context. This journal produced a place for discussions and debates that contributed for controlling and producing symbolic resources

Paixão, mito e formas de vida em textos publicitários

The passion, understood by Aristotle, as a contingency which causes a change in the essence of the subject, was object of research of the Greek philosopher as rhetorical strategy used by the speaker to touch its listeners. The greimasian semiotics, enlarging the concept of Aristotle’s passion, understands it as the soul states of a subject that can be gathered form the analysis of a text. Reflections towards the passion discursivization and its persuasion effects in advertisements lead us to establish the relationships between passion, myth and ways of life.

Cidadania e nacionalidade como formas de exclusão

O presente trabalho versa sobre o acesso de estrangeiros ao território nacional. Essa limitação restringe direitos fundamentais da maioria dos imigrantes, além de expô-los a uma condição de vulnerabilidade que permite que sejam explorados pelo setor econômico de produção e perseguidos pela polícia. O Estado não costuma ver o imigrante como “vítima”, mas como marginal”. Se todos os homens são fundamentalmente iguais em razão de sua igual dignidade, se a constante comunicação e movimentação são imprescindíveis e se é moralmente impossível justi*car os privilégios de pessoas porque nasceram em determinado território ou de determinados genitores, então as funções discriminadoras dos conceitos de cidadania e nacionalidade perdem sua justificativa.

Novas formas de organização da produção canavieira na região de Ribeirão Preto/SP: qualificação profissional e o Programa Renovação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Carnaval X Entrudo: formas de regrar o carnaval no Rio de Janeiro em fins do Século XIX e Início do Século XX

This article aims to discuss the attempts of the authorities in Rio de Janeiro to regulate Carnival festivities and discipline the revelers. Some of the ways to do this in the late nineteenth and early twentieth centuries, are connected to the prohibition of Shrovetide and of the wearing of some costumes by individual masked men, such as Indian and imp costumes, which are associated with backwardness and barbarism, and, therefore, with a period of history that part of the intelligentsia of Rio de Janeiro wanted to forget. Such manifestations were at odds with political, social and cultural transformations, and with the image of modernity that the city of Rio de Janeiro wanted to consolidate in that period.

Uma proposta para o 6ºano do EF: primeiras formas de geometria espacial-construindo conceitos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Geocronologia de formas deposicionais e evolução da planície interleques do Rio Negro, quartenário da Bacia do Pantanal

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Formas de vida da mulher brasileira no discurso jurídico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Brasil, Moçambique e Angola: desvendando relações sociolinguísticas pelo prisma das formas de tratamento

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Obesidade infantil: etiologia, consequências e formas de intervenção

Obesity is defined as excess body fat. It is considered today a serious public health problem and has reached high proportions in childhood and adolescence. Among factors highlight the lifestyle, poor diet and little physical activity for their development. Childhood obesity is not treated can become permanent in adulthood increases the risk of several diseases such as cardiovascular, metabolic and other disorders such as depression, low self-esteem among others. It's extreme need multidisciplinary intervention to prevent it, with guidelines on healthy eating habits and regular physical activity. Treatment is necessary in a multidisciplinary team of doctors, nutritionists, physical educators, psychologists and family that has a decisive weight in the treatment of obese children. The media could give more space for the disclosure of childhood obesity, so that the population is aware of this epidemic in a more serious understand its risks and health effects. This bibliographic study has character and aims to seek the causes of obesity, its consequences in adulthood and possible forms of intervention

Efeitos de doses e formas de aplicação de reguladores de crescimento em uvas sem sementes, cv. BRS Clara, em região tropical

O objetivo deste trabalho foi avaliar o efeito do uso dos reguladores vegetais, ácido giberélico e tidiazuron, no tamanho de bagas da cultivar BRS Clara em Região Tropical. Os experimentos foram conduzidos em 2006 e 2007, na Estação Experimental de Viticultura Tropical, em Jales-SP, região noroeste do Estado de São Paulo, em plantas da cv. BRS Clara. Foram testadas diferentes concentrações de GA3 aplicadas isoladamente, combinadas com Crop Set ou tidiazuron, em aplicações únicas ou sequênciais (2 e 4 vezes). As avaliações foram feitas por ocasião da maturação das uvas, considerando-se a massa fresca dos cachos, dos engaços e das bagas, determinadas por meio de balança analítica; o comprimento e o diâmetro médio das bagas, utilizando-se de paquímetro; e o teor de sólidos solúveis (SS), por meio de refratômetro manual. Os experimentos foram conduzidos em delineamento experimental de blocos casualizados, representados por uma planta, com 15 repetições, no ano de 2006, e 10 repetições, no ano de 2007. Os dados foram submetidos à análise de variância e, para a comparação das médias dos tratamentos, foi utilizado o teste Skott Nott, ao nível de 5% de probabilidade. Observou-se que o uso dos reguladores promoveu o crescimento das bagas na cultivar BRS Clara em todos os tratamentos, diferindo da testemunha.