147 resultados para Extrações seriadas


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Pós-graduação em Ciência Animal - FMVA

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Pós-graduação em Agronomia (Horticultura) - FCA

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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The repetition should be understood as the property of mass cultural products, originated from the need to minimize the dispersion of the audience in their enjoyment. Based on this assumption, this paper measures the rate of iteration manifested in the scripts of the following serial fictions: Duas Caras, A Grande Família and House of Cards. Established itself as a method of scene analysis proposed by McKee (2008), questioned whether the lack of classical education of the public and rescued the forms assumed by repeating the melodrama, romance-serial and dramaturgy: asides, monologues, confidences and planar scenes. The proposal was applied in two scripts for each of the aforementioned productions. Considering the data collected, it appears that there was reduction in repeat products offered on demand thanks to the possibility of handling the flow and the attention given receptor.

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Pós-graduação em Medicina Veterinária - FCAV

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The identification of Salmonella spp. in food samples by microbiological diagnosis is time consuming, with approximately five different stages, requiring about 120 hours until the final result. The utilization of the polymerase chain reaction technique (PCR) can reduce this time, but substances present in samples may affect the reaction. The present work aimed to compare DNA extraction by thermic treatment and by the use of cetyltrimethil ammonium bromide (CTAB), in products originated from poultry houses corresponding to raw material (meat meal) and experimentally contaminated drag swabs. Materials obtained from the extractions were submitted to PCR, utilizing a pair of initiator oligonucleotides for amplification of Sdf 1 gene fragments. Comparing the methods of extraction, it was observed that when CTAB was employed, SE was detected in 70% of meat meal and in 80% of drag swabs, while the thermic treatment method yielded positive results in 20% of meat meal and in 40% of drag swabs. SE was detected under both methods utilized for DNA extraction, but the use of CTAB detected a greater number of positive samples, compared with thermal treatment.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)