Investigação da amplificação do EGFR em carcinoma de células escamosas de boca em pacientes jovens

Pós-graduação em Biopatologia Bucal - ICT

Investigação da amplificação do EGFR em carcinoma de células escamosas de boca em pacientes jovens

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Mapeamento de ocorrências de eventos perigosos no município de Santos SP: integração com dados de vulnerabilidade socioambiental

This study is based on the mapping of occurrences associated with social vulnerability and natural risks, which refer to the resilience of populations and territories, regarding natural hazards associated with the functioning of natural systems (eg, earthquakes, flood, mass movements). According to UNISDR (2014), the state of São Paulo is a reference in working with Urban Resilience and Disaster in the Resilient Cities Campaign (2014), considering the high investment in Risk Areas Mapping and Public Education Campaigns implemented by the Civil Defense. Thus, this study aims to mapping the occurrence of events related to Tree Falls, Erosion, Landslides, Irregular Housing, Rocky Blocks Falls, Wall Falls, Unroofing and Irregular Construction, attended by the Civil Defense in the city of Santos, São Paulo State, from 2011 to 2014. Thereafter, correlated analyzes to the Environmental Vulnerabilities were generated. The Environmental Vulnerability databases used in this dissertation compose the results of the CNPQ Project - Environmental Vulnerability Mapping of the State of Sao Paulo - Brazil: a methodological contribution of Freitas (2013) and Bortolettoet al (2014), with information collected from the 2010 Census (IBGE, 2010), on a census sectors scale. The adopted methodological procedure involves document analysis followed by data integration in Geographic Information System, through algorithms analysis and mapscrossing.The results obtained in Maps of Social and Environmental Vulnerability Occurrences presented areas of High or Very High Vulnerability. The main variables obtained with such characteristics are Irregular Housing, Landslides and Rocky Blocks Falls, which was associated with hilly terrain formations, with slopes above 30%. To the areas of Medium, Low and Very Low Vulnerability were associated the variables Tree Falls, Wall Falls, Erosion, Unroofing and Irregular Construction, which are spatially distributed without an...

Role of ATM and PTEN in prostatic carcinogenic dog prostate: validation of aCGH results

The canis lupus familiares is the only species besides human that spontaneously develop prostatic carcinoma (PCa). In addition, the metastatic sites are similar to those frequently reported in men. For these reasons, the dog is the best natural model to study the molecular mechanisms in PCa development providing a natural animal model for treatment by molecular targets. Previously, we investigated copy number alterations by arrayCGH (Canine Genome CGH Microarray 4x44K-G2519F, Agilent Technologies) in canine prostatic lesions: 3 benign prostatic hyperplasias (BPH), 4 proliferative inflammatory atrophies (PIA), and 14 PCa. Five histologically normal prostatic tissues were used as reference. Genomic alterations were evaluated using Genomic Workbench Standard Edition 5.0.14. This previous study revealed significant copy number losses of Atm and Pten exclusively in PCa. In the present study, ATM and PTEN immunoexpression were investigated using a tissue microarray (TMA) containing 149 canine prostatic paraffin-embedded lesions (BPH, PIA and PCa) collected from 67 animals. Immunohistochemical reactions were performed using the polyclonal rabbit antibody anti-PTEN (Santa Cruz Biotech, 1:50) and anti-ATM (Abcam, 1:50). The sections were developed with diaminobenzidine (DAB) and peroxidase. The immunohistochemical staining was assessed in each core by the distribution of positive cells for each antibody per lesion (score 1: <25% cells positive, 2: 26% to 50%, 3: being 51% and 75% and 4:> 75%) and intensity (1: weak, 2: moderate, 3: intense). Chi-square or Fisher exact test was used to determine the association between the categorical variables using GraphPad Prism 5 (GraphPad Software Inc., La Jolla, CA). Distribution of positive cells did not differ among lesions. PCa and PIA showed more samples with weak intensity for ATM when compared to normal prostatic tissue and BPH (PCa: p=0,032 and PIA: p=0,025). Benign prostatic hyperplasia and normal samples presented intense PTEN immunostaining than PCa (p=0,021) and PIA (p=0,0013). These results suggest that ATM and PTEN proteins expression in canine prostatic carcinoma are downregulated possibly by copy number losses. These findings are similar from those described in prostate carcinomas from human corroborating for the use of dogs as a natural model to study prostatic disease in men.

Avaliação do gasto energético de repouso em pacientes com sepse associada ou não à lesão renal aguda

Pós-graduação em Fisiopatologia em Clínica Médica - FMB

Mapeamento geológico-geotécnico por meio de análise integrada no Parque Estadual do Juquery - Franco da Rocha-SP

Pós-graduação em Geociências e Meio Ambiente - IGCE

Contribuição metodológica ao estudo da dissecação e energia do relevo: proposta e avaliação de técnicas computacionais

Pós-graduação em Geociências e Meio Ambiente - IGCE

As características da temperatura do ar em bairros com produção do espaço urbano distintas: uma análise do Parque Higienópolis e Jd. Morada do Sol em Presidente Prudente-SP

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Vulnerabilidade às inundações em Teresina, Piauí

Pós-graduação em Geografia - IGCE

O professor de biologia e a possibilidade de uso de diferentes abordagens de ensino com materiais midiáticos a fim de desenvolver a criticidade do aluno do ensino médio

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)