Síntese e caracterização de copolímeros de polietilenoglicol monometil éter em quitosana cationizada para futuras aplicações em biotecnologia

Pós-graduação em Química - IQ

Desenvolvimento de sensores descartáveis de ouro nanoestruturado modificados com complexo metálico visando detecção in situ de corantes semipermanentes de cabelo

Pós-graduação em Química - IQ

Purification and biochemical characterization of an alkaline pectin lyase from Fusarium decemcellulare MTCC 2079 suitable for Crotolaria juncea fiber retting

An extracellular pectin lyase secreted by Fusarium decemcellulare MTCC 2079 under solid state fermentation condition has been purified to electrophoretic homogeniety by using ammonium sulfate fractionation, carboxymethyl cellulose and gel filtration (Sephadex G-100) column chromatographies. The purified enzyme showed single protein band corresponding to molecular mass 45 +/- 01 kDa on sodium dodecyl sulfate polyacrylamide gel electrophoresis. The enzyme had maximum activity at pH 9.0 and showed maximum stability in the pH range of 9.0-12.0. The optimum temperature of the purified enzyme was 50 degrees C and it showed maximum stability upto 40 degrees C. The energy of activation for the thermal denaturation (Ea) was 59.06 kJ mol(-1) K-1. The K-m and k(cat) values using citrus pectin as the substrate were 0.125mgml(-1) and 72.9 s(-1) in 100mM sodium carbonate buffer pH 9.0 at 50 degrees C. The biophysical studies on pectin lyase showed that its secondary structure belongs to alpha+beta class of protein with comparatively less of beta-sheets. Purified pectin lyase showed efficient retting of Crotolaria juncea fibers.

Development of hydrophilic nanocarriers for the charged form of the local anesthetic articaine

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desenvolvimento de nanopartículas de quitosana para adminstração nasal de zidovudina

Zidovudine (AZT) is the drug most commonly used in AIDS treatment, isolated or in combination with other antiretroviral agents, but it has certain limitations due to its therapeutic dose-dependent haematological toxicity. In addition, it has low oral bioavailability, since it undergoes pre-systemic metabolism. The nasal route has been used as an alternative route for drug administration, because it can promote its direct absorption to blood circulation, avoiding hepatic metabolism. However, this route presents as a factor limiting the mucociliary clearance mechanisms that remove quickly the formulation of the nasal cavity. To prolong the residence time of formulations, in this direction, has been proposed the development of mucoadhesive systems. Among the various existing systems, the use of chitosan (QS), as mucoadhesive polymer, has been widely exploited in the preparation of nanoparticles (NPs). The objective of this study was to develop and characterize QS’s NPs for intranasal administration of AZT. For both NPs have been developed by ionic crosslinking of QS with sodium tripolyphosphate (TPP). These NPs were characterized by studies of particle size distribution, zeta potential, morphology, mucoadhesion tests, assessing the ability of encapsulation of the drug and permeation profile of AZT. The evaluation of AZT in the NPs was determined by UV-Vis spectroscopy. Mucoadhesion measures were made using a texture analyzer, using a mucin disk and porcine mucous membrane , and permeation assay were conducted using porcine nasal mucous membrane adapted to the Franz cell. These results suggest that the systems in hand have great potential for nasal AZT administration

Síntese de microesferas de quitosana para aplicação em liberação controlada de drogas pelo método de atomização e coagulação

Chitosan is a natural biodegradable polymer with great potential for pharmaceutical applications due to its biocompatibility, high charge density , nontoxicity and mucoadhesion. Gel formation can be obtained by the interactions of chitosans with low molecular counterions such as polyphosphates, sulphates and crosslinking with glutaraldehyde. This gelling property of chitosan allows a wide range of applications such as coating of pharmaceuticals and food products, gel entrapment of biochemicals, whole cells, microorganisms and algae. One of its main applications is the synthesis of microspheres for coating of pharmaceuticals , magnetic particles an other substances. In such a way, we can build targeted drug delivery systems. In the present work, we applied the method of spraying and coagulation. The resulting microspheres, then, were characterized by optical microscopy

Produção de polpa solúvel a partir de Eucalyptus urograndis

The wood of the gender Eucalyptus occupies a prominence place in relation to other cellulose sources, due to its chemical composition, its low cost, abundance and availability. The dissolving pulp obtaining occurs basically starting from three stages: prehydrolysis of the wood following by cooking and finally by the bleaching process. In the dissolving pulp production is necessary a pretreatment stage for the hemicelluloses removal. Among the chemical cooking processes, the Kraft process is the most used for cellulosic pulps production starting from eucalyptus, in the additive presence or not. The anthraquinone uses in the pulping process has as main purposes increasing the delignification rate as well as protecting the pulp regarding the degradation of the polysaccharide chains. The ECF bleaching process consists of the purification of the pulp through bleaching agents, being excepted the elementary chlorine. The dissolving pulp is a pulp of high a-cellulose content and purity destined to the cellulose derivatives production as carboxymethyl cellulose, nitrocellulose and microcrystalline cellulose. The production of a certain product, as well as its final application, is determined by the chemical properties of the pulp. The present work had as purpose producing cellulosic pulp with kappa number around 9 destined to the production of dissolving pulp in way to evaluate the use of the anthraquinone (AQ) in the Kraft process according to the chemical properties of the obtained pulps. It has been still intended developing an effective technology of bleaching for the high purity and quality dissolving pulp production. The prehydrolysis was accomplished in laboratory rotational autoclave for 30 minutes at 170ºC in the water:wood rate 3,5:1 (L:kg). The Kraft/AQ pulping were accomplished in laboratory rotational autoclave being initially modified the active alkali load (10, 13, 16 and 19%) at sulfidity of 25% ...

Desenvolvimento de imunossensor baseado na imobilização de anticorpo monoclonal em fibroína da seda para diagnóstico rápido da cisticercose bovina

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Imobilização da enzima álcool desidrogenase em suportes alginato-quitosana e glioxil-agarose

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo comparativo de citocinas anti-inflamatórias em células tronco mesenquimais cultivadas com Cordia ecalyculata e biocurativo

Pós-graduação em Pesquisa e Desenvolvimento (Biotecnologia Médica) - FMB

Properties of films obtained from biopolymers of different origins for skin lesions therapy

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influência de polímeros bioadesivos sobre o efeito protetor do flúor no desenvolvimento da erosão dental

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Matrizes de quitosina-colágeno podem regular as atividades biológicas de células tronco mensenquimais adiposas para a engenharia de tecidos

Pós-graduação em Biofísica Molecular - IBILCE

Estudo físico-químico da atividade fungicida de derivados anfifílicos de quitosana contra fungos do gênero Aspergillus : interação com modelos de membranas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desenvolvimento de filmes biodegradáveis à base de zeína, caracterização das propriedades funcionais e estruturais e avaliação do uso como cobertura na conservação das características físico-químicas do queijo Minas padrão

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)