Light-related photosynthetic characteristics of lotic macroalgae

Photosynthetic characteristics in response to irradiance were analysed in 42 populations of 33 macroalgal species by two distinct techniques (chlorophyll fluorescence and oxygen evolution). Photosynthesis-irradiance (PI) curves based on the two techniques indicated adaptations to low irradiance reflected by low saturation values, high to moderate values of photosynthetic efficiency (alpha) and photoinhibition (beta), for Bacillariophyta and Rhodophyta, which suggests they are typically shade-adapted algae. In contrast, most species of Chlorophyta were reported as sun adapted algae, characterized by high values of I-k and low of alpha, and lack of or low photoinhibition. Cyanophyta and Xanthophyta were intermediate groups in terms of light adaptations. Photoinhibition was observed in variable degrees in all algal groups, under field and laboratory conditions, which confirms that it is not artificially induced by experimental conditions, but is rather a common and natural phenomenon of the lotic macroalgae. Low values of compensation irradiance (I-c) were found, which indicate that these algae can keep an autotrophic metabolism even under very low irradiances. High ratios (>2) of photosynthesis/respiration were found in most algae, which indicates a considerable net gain. These two physiological characteristics suggest that macroalgae may be important primary producers in lotic ecosystems. Saturation parameters (I-k and I-s) occurred in a relatively narrow range of irradiances (100-400 mumol photons m(-2) s(-1)), with some exceptions (higher in some filamentous green algae or lower in red algae). These parameters were way below the irradiances measured at collecting sites for most algae, which means that most of the available light energy was not photochemically converted via photosynthesis. Acclimation to ambient PAR was observed, as revealed by lower values of I-k and I-c and higher values of alpha and quantum yield in algae from shaded streams, and vice versa. Forms living within the boundary layer (crusts) showed responses of shade-adapted species and had the highest values of P-max, alpha and quantum yield, whereas the opposite trend was observed in gelatinous forms (colonies and. laments). These results suggests adaptation to the light regime rather than functional attributes related to the growth form.

Formação de biofilmes e resistência a antifúngico e biocidas em Candida parapsilosis e C. orthopsilosis isoladas de águas usadas para hemodiálise

Pós-graduação em Biociências e Biotecnologia Aplicadas à Farmácia - FCFAR

Do woody and herbaceous species compete for soil water across topographic gradients? Evidence for niche partitioning in a Neotropical savanna

Savannas are characterized by sparsely distributed woody species within a continuous herbaceous cover, composed mainly by grasses and small eudicot herbs. This vegetation structure is variable across the landscape, with shifts from open grassland to savanna woodland determined by factors that control tree density. These shifts often appear coupled with environmental variations, such as topographic gradients. Here we investigated whether herbaceous and woody savanna species differ in their use of soil water along a topographic gradient of about 110 m, spanning several vegetation physiognomies generally associated with Neotropical savannas. We measured the delta H-2 and delta O-18 signatures of plants, soils, groundwater and rainfall, determining the depth of plant water uptake and examining variations in water uptake patterns along the gradient. We found that woody species use water from deeper soil layers compared to herbaceous species, regardless of their position in the topographic gradient. However, the presence of a shallow water table restricted plant water uptake to the superficial soil layers at lower portions of the gradient. We confirmed that woody and herbaceous species are plastic with respect to their water use strategy, which determines niche partitioning across topographic gradients. Abiotic factors such as groundwater level, affect water uptake patterns independently of plant growth form, reinforcing vegetation gradients by exerting divergent selective pressures across topographic gradients. (C) 2013 SAAB. Published by Elsevier B.V. All rights reserved.

Random regression analyses using B-splines functions to model growth from birth to adult age in Canchim cattle

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Obtaining of Nanoapatite in Ti-7.5Mo Surface After Nanotube Growth

Titanium and their alloys have been used for biomedical applications due their excellent mechanical properties, corrosion resistance and biocompatibility. However, they are considered bioinerts materials because when they are inserted into the human body they are cannot form a chemical bond with bone. In several studies, the authors have attempted to modify their characteristic with treatments that changes the material surface. The purpose of this work was to evaluate obtaining of nanoapatite after growing of the nanotubes in surface of Ti-7.5Mo alloy. Alloy was obtained from c.p. titanium and molibdenium by using an arc-melting furnace. Ingots were submitted to heat treatment and they were cold worked by swaging. Nanotubes were processed using anodic oxidation of alloy in electrolyte solution. Surfaces were investigated using scanning electron microscope (SEM), FEG-SEM and thin-film x-ray diffraction. The results indicate that nanoapatite coating could form on surface of Ti-7.5Mo experimental alloy after nanotubes growth.

Dynamic Scaling and Growth Kinetics of 3-Glycidoxypropyltrimethoxysilane-Derived Organic/Silica Hybrids

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

On the reversed crystal growth of BaZrO3 decaoctahedron: shape evolution and mechanism

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Longitudinal study of mandibular behavior in Class I subjects with vertical and horizontal growth

OBJETIVO: avaliar as possíveis diferenças no comportamento mandibular em indivíduos Classe I com crescimento vertical e horizontal. MÉTODOS: a amostra desse estudo consistiu de 20 indivíduos Classe I não tratados, sendo o grupo 1 composto por 10 indivíduos com padrão de crescimento vertical e o grupo 2 por 10 indivíduos com padrão de crescimento horizontal, pertencentes aos arquivos do Burlington Growth Center, University of Toronto, no Canadá, acompanhados radiograficamente nas idade de 9, 12 e 21 anos. Determinou-se, por meio de telerradiografias cefalométricas, em norma lateral, os valores médios para a avaliação longitudinal do comportamento da mandíbula utilizando as medidas SNB, Co-Gn, SN.GoMe, altura facial anterior e altura facial posterior. RESULTADOS: o valor de SNB e Co-Gn foram maiores no grupo com crescimento horizontal em todas as idades. A medida Sn.GoMe foi significativamente menor no grupo com crescimento horizontal, a altura facial anterior (AFH) apresentou valores menores nos indivíduos com padrão de crescimento horizontal, e a altura facial posterior (PFH) apresentou valores menores nos indivíduos com crescimento vertical. CONCLUSÃO: as comparações longitudinais das tendências de crescimento de indivíduos Classe I indicam que existe diferenças significativas entre os dois grupos. A mandíbula apresentou tendência à rotação horária no grupo 1. O grupo 2 exibiu tendência à característica de indivíduos braquicefálicos, na forma facial, devido ao déficit no desenvolvimento vertical na altura facial anterior.

Effect of sugar catabolite repression in correlation with the structural complexity of the nitrogen source on yeast growth and fermentation

Biomass and ethanol production by industrial Saccharomyces cerevisiae strains were strongly affected by the structural complexity of the nitrogen source during fermentation in media containing galactose, and supplemented with a nitrogen source varying from a single ammonium salt (ammonium sulfate) to free amino acids (casamino acids) and peptides (peptone). Diauxie was observed at low galactose concentrations independent of nitrogen supplementation. At high sugar concentrations altered patterns of galactose utilisation were observed. Biomass accumulation and ethanol production depended on the nature of the nitrogen source and were different for baking and brewing ale and lager strains. Baking yeast showed improved galactose fermentation performance in the medium supplemented with casamino acids. High biomass production was observed with peptone and casamino acids for the ale brewing strain, however high ethanol production was observed only in the presence of casamino acids. Conversely, peptone was the nitrogen supplement that induced higher biomass and ethanol production for the lager brewing strain. Ammonium salts always induced poor yeast performance. The results with galactose differed from those obtained with glucose and maltose which indicated that supplementation with a nitrogen source in the peptide form (peptone) was more positive for yeast metabolism, suggesting that sugar catabolite repression has a central role in yeast performance in a medium containing nitrogen sources with differing levels of structural complexity.

Structural complexity of the nitrogen source and influence on yeast growth and fermentation

The structural complexity of the nitrogen source strongly affects both biomass and ethanol production by industrial strains of Saccharomyces cerevisiae, during fermentation in media containing glucose or maltose, and supplemented with a nitrogen source varying from a single ammonium salt (ammonium sulfate) to free amino acids (casamino acids) and peptides (peptone). Diauxie was observed at low glucose and maltose concentrations independent of nitrogen supplementation. At high sugar concentrations diauxie was not easily observed. and growth and ethanol production depended on the nature of the nitrogen source. This was different for baking and brewing ale and lager yeast strains. Sugar concentration had a strong effect on the shift from oxido-fermentative to oxidative metabolism. At low sugar concentrations, biomass production was similar under both peptone and casamino acid supplementation. Under casamino acid supplementation, the time for metabolic shift increased with the glucose concentration, together with a decrease in the biomass production. This drastic effect on glucose fermentation resulted in the extinction of the second growth phase, probably due to the loss of cell viability. Ammonium salts always induced poor yeast performance. In general, supplementation with a nitrogen source in the peptide form (peptone) was more positive for yeast metabolism, inducing higher biomass and ethanol production, and preserving yeast viability, in both glucose and maltose media, for baking and brewing ale and lager yeast strains. Determination of amino acid utilization showed that most free and peptide amino acids present, in peptone and casamino acids, were utilized by the yeast, suggesting that the results described in this work were not due to a nutritional status induced by nitrogen limitation.

The ultrastructural aspects of vitellogenesis or oocyte secondary growth in Serrasalmus spilopleura (Teleostei, Characiformes, Serrasalminae)

Oocyte secondary growth in S. spiloleura corresponds to the period in which different vesicular structures are formed, including the cortical alveoli and the yolk granules. The oocytes with cortical alveolus formation show vesicular structures with filamentous content in the cortical cytoplasmic region, which are the cortical alveolus precursors. In these oocytes, electron-dense vesicles of heterogenous content are dispersed in the inner cytoplasmic region and their nuclei are irregular, showing many nucleoli of different sizes. The oocytes in vitellogenesis are filled with many vesicles. The cortical alveolus precursors are in the peripheral region, and electron-dense granules are seen near to the nucleus. These fuse and form yolk granules. The oocytes in vitellogenesis show a very irregular nucleus that has nucleoli of different sizes. In the oocytes in final vitellogenesis, the yolk granules are scattered throughout the cytoplasm, displacing the cortical alveoli toward cell periphery. The nucleus is similar to the other stages.

Eumelanin thin films: Solution-processing, growth, and charge transport properties

Eumelanin pigments show hydration-dependent conductivity, broad-band UV-vis absorption, and chelation of metal ions. Solution-processing of synthetic eumelanins opens new possibilities for the characterization of eumelanin in thin film form and its integration into bioelectronic devices. We investigate the effect of different synthesis routes and processing solvents on the growth, the morphology, and the chemical composition of eumelanin thin films using atomic force microscopy and X-ray photoelectron spectroscopy. We further characterize the films by transient electrical current measurements obtained at 50% to 90% relative humidity, relevant for bioelectronic applications. We show that the use of dimethyl sulfoxide is preferable over ammonia solution as processing solvent, yielding homogeneous films with surface roughnesses below 0.5 nm and a chemical composition in agreement with the eumelanin molecular structure. These eumelanin films grow in a quasi layer-by-layer mode, each layer being composed of nanoaggregates, 1-2 nm high, 10-30 nm large. The transient electrical measurements using a planar two-electrode device suggest that there are two contributions to the current, electronic and ionic, the latter being increasingly dominant at higher hydration, and point to the importance of time-dependent electrical characterization of eumelanin films. This journal is © 2013 The Royal Society of Chemistry.

Linguagem poética e crescimento urbano-industrial / Poetical language and urban-industrial growth

O impacto do crescimento urbano-industrial sobre a lírica moderna é abordado sob o ângulo das dissonâncias ocorridas entre as representações temáticas e os procedimentos expressivos. Assim se confrontam as “respostas” dos poetas europeus à Revolução Industrial e o surgimento das grandes cidades com as dos modernistas brasileiros de 22 face à industrialização de São Paulo. Nestes, ao contrário dos primeiros, os conflitos instauram-se entre a celebração programática da vida moderna e, num primeiro momento, a impossibilidade de adequação de suas formas de expressão ao registro dessa modernidade. Dessa forma se explicam os desequilíbrios temático-expressivos existentes em Paulicéia Desvairada, bem como as adequações conseguidas por obras posteriores. The impact of urban-industrial growth on modern lyrics is approached from the angle of the dissonances which occurred between the thematic representations and the expressive procedures. Thus, the European poets’ responses to the Industrial Revolution and the emergence of the large cities are confronted with those of the 1922 Brazilian Modernists in relation to the industrialization of São Paulo. In the latter, contrary to what happened with ther former ones, the conflicts take place between the programmatic celebration of modern life and, at first, the impossibility of adequating their form of expressions to the record of said modernity. Thus the thematic-expressive disequilibrium in Paulicéia Desvairada, as well as de adequations obtained in later works are explained.

Mechanisms of SnO2 Nanoparticles Formation and Growth in Acid Ethanol Solution Derived from SAXS and Combined Raman-XAS Time-Resolved Studies

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Phenotypic and functional evaluations of peripheral blood monocytes from chronic-form paracoccidioidomycosis patients before and after treatment

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)