Precision of distances and ordering of microsatellite markers in consensus linkage maps of chromosomes 1, 3 and 4 from two reciprocal chicken populations using bootstrap sampling

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The influence of landscape characteristics and home-range size on the quantification of landscape-genetics relationships

A common approach used to estimate landscape resistance involves comparing correlations of ecological and genetic distances calculated among individuals of a species. However, the location of sampled individuals may contain some degree of spatial uncertainty due to the natural variation of animals moving through their home range or measurement error in plant or animal locations. In this study, we evaluate the ways that spatial uncertainty, landscape characteristics, and genetic stochasticity interact to influence the strength and variability of conclusions about landscape-genetics relationships. We used a neutral landscape model to generate 45 landscapes composed of habitat and non-habitat, varying in percent habitat, aggregation, and structural connectivity (patch cohesion). We created true and alternate locations for 500 individuals, calculated ecological distances (least-cost paths), and simulated genetic distances among individuals. We compared correlations between ecological distances for true and alternate locations. We then simulated genotypes at 15 neutral loci and investigated whether the same influences could be detected in simple Mantel tests and while controlling for the effects of isolation-by distance using the partial Mantel test. Spatial uncertainty interacted with the percentage of habitat in the landscape, but led to only small reductions in correlations. Furthermore, the strongest correlations occurred with low percent habitat, high aggregation, and low to intermediate levels of cohesion. Overall genetic stochasticity was relatively low and was influenced by landscape characteristics.

Tamanho de amostra e estimativa de custo em levantamento epidemiológico de cárie dentária

This study aimed to analyze how the prevalence and the distribution of dental caries influence the sample size in epidemiological surveys, and how much are the costs. Secondary data of oral health surveys in 12-year-old schoolchildren from Bauru in 1976, 1984, 1990, 1994, and 2001, and from Piracicaba in 2001 and 2005 were studied. Sample sizes were estimated taking into account the mean DMFT and standard deviation of each survey, establishing sampling errors of 1%, 2%, 5%, and 10%. Costs were estimated considering permanent material, consumption material and human resources. The sample size in both towns needed to be increased, ranging from 119 in 1976 to 1,118 in 2001 in Bauru, and from 954 in 2001 to 1,252 in 2005 in Piracicaba, when a sampling error of 10% was considered. The cost of dental caries surveys was verified considering different sampling errors. This cost depends on how acceptable is the margin of difference between the true mean and the one found in the survey. In conclusion, the reduction in the prevalence of dental caries has determined the need for increase in sample size and in costs for conducting the surveys.

Unidade amostral para determinação de massa média de frutos em lichieira em sistema orgânico e convencional

The experimental unit of measurement is a suitable technique to estimate the average character in evaluation, and experimentation should be well understood and executed, because the precision characterizes the quality of the inferences of the results. This research aimed to analyze the possibility to obtaining the quantity of fruit required for sampling of lychee plants, to determining the average mass of the fruits, with less sampling error. One hundred fruits were collected, in a hectare, in two cropping systems, conventional and organic, being one fruit per plant. From the data of fruit mass was used the Excel® to calculate the average fruit mass and number of fruits samples needed to represent the area of the producer. The results show that to achieve a sampling error of 10% for determining the average mass of fruit, 400 fruits should be collected for organic system and 370 for the conventional, while for 20% sampling error, it is necessary to collect 250 fruits per hectare in organic system and 220 in the conventional.

Amostragem de folhas em cafeeiro para fins de diagnose do estado nutricional

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

Correlating soil porosity and respective geological unit in Paraíba do sul Valley, Brazil: a geostatistical methodology proposal

This manuscript aims proposing a methodology for correlating soil porosity to the respective geological units using geostatistical analysis techniques, including interpolation data by kriging. The site studied was in Lorena municipality, Paraíba do Sul Valley, southeastern Brazil. Specifically all studies were carried out within an area of 12 km2 located at Santa Edwirges farm. The database comprehended 41 soil samples taken at different geological and geomorphologic units at three different depths: surface, 50 cm and 100 cm depth. The geostatistical analyses results were correlated to a geological mapping specifically elaborated for the site. This mapping accounts for two different geological formations and a geological contact characterized by a shearing zone. The results indicate the existence of a significant relationship between the soil porosity and the respective geological units. The studies revealed that the residual soils from weathered granitic rocks tend to have higher porosities than the residual soils from weathered biotite gneiss rocks, while the soil porosity within the shearing zone is relatively un-sensitive to the respective geological formation. The spatial patterns observed were efficient to evaluate the relationship between the soil porosity, geology unit and the and geomorphology showing a good potential for correlating with others soil properties such as hydraulic conductivity, soil water retention curves and erosion potentials.

Capacidade funcional de idosos dependentes cadastrados na estratégia saúde da família do município de Londrina,PR

Pós-graduação em Saúde Coletiva - FMB

The carpal pad as an alternative sampling site for blood glucose testing in dogs

To investigate the feasibility and validity of sampling blood from the carpal pad in hospitalised healthy and diabetic dogs. METHODS The carpal pad was compared to the ear as a sampling site in 60 dogs (30 healthy and 30 diabetic dogs). RESULTS Lancing the pads was very well tolerated. The average glucose concentrations in blood samples obtained from the ears and carpal pads exhibited a strong positive correlation (r = 0.938) and there were no significant differences between them (P = 0.914). In addition, 98.3% of the values obtained were clinically acceptable when assessed by the error grid analysis. CLINICAL SIGNIFICANCE The carpal pad is a good alternative sampling site for home monitoring, especially in animals with a soft and/or light-coloured pad.

Influence of uncorrected refractive error and unmet refractive error on visual impairment in a Brazilian population

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A new sampling strategy for the Shewhart control chart monitoring a process with wandering mean

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Planos de amostragem seqüenciais para Orthezia praelonga Douglas (Hemiptera: Sternorrhyncha, Ortheziidae) na cultura de citros

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Dimensionamento de amostras para monitoramento do ácaro da leprose Brevipalpus phoenicis (Geijskes, 1939) em citros

O controle do ácaro Brevipalpus phoenicis (Geijskes, 1939), transmissor do vírus da leprose, deve ser realizado quando sua população atinge o nível de ação, obtido pelo monitoramento de sua população, por meio de amostragens. Objetivou-se determinar o tamanho da amostra aceitável para estimar a população do ácaro, para posterior tomada de decisão. O experimento foi realizado na Fazenda Cambuhy, Matão - SP, no ano agrícola 2003-2004. Escolheu-se ao acaso um talhão da variedade Valência, com oito anos de idade, plantada no espaçamento 7x3,5m, com 2.480 plantas. Nesse talhão, foram inspecionados 1; 2; 3; 5; 10 e 100% das plantas, o que corresponde a 25; 50; 74; 124; 248 e 2.480 plantas, respectivamente, em caminhamento no sentido das linhas de plantio. Foram amostrados 3 frutos ou, na ausência destes, eram analisados ramos. de acordo com os resultados obtidos, observa-se que a porcentagem de erro na estimativa da média para a porcentagem de frutos com presença de ácaros, quando se amostra apenas 1% das plantas (25 plantas), é de 50%, ou seja, para uma infestação de 10%, a variação da porcentagem de frutos infestados estaria entre 5 e 15%, levando o produtor a subestimar ou a superestimar o nível de infestação, aumentando os gastos com pulverizações desnecessárias ou um controle ineficiente do ácaro. Para que o erro na amostragem fique dentro da situação aceitável, de 20 a 30% (em média 25%) de erro, deveriam ser amostradas 105 plantas. Na porcentagem de frutos com mais de 10 ácaros, verifica-se que, para a situação aceitável (20 a 30%), devem ser inspecionadas 540 plantas.

Amostragem para diagnose do estado nutricional de mangueiras

Dentre todas as etapas que permeiam um laudo foliar, ainda a amostragem continua sendo a mais sujeita a erros. O presente trabalho teve como objetivo determinar o tamanho de amostras foliares e a variação do erro amostral para coleta de folhas de pomares de mangueiras. O experimento contou com delineamento inteiramente casualizado, com seis repetições e quatro tratamentos, que constaram da coleta de uma folha, em cada uma das quatro posições cardeais, em 5; 10; 20 e 40 plantas. Com base nos resultados dos teores de nutrientes, foram calculados as médias, variâncias, erros-padrão das médias, o intervalo de confiança para a média e a porcentagem de erro em relação à média, através da semi-amplitude do intervalo de confiança expresso em porcentagem da média. Concluiu-se que, para as determinações químicas dos macronutrientes, 10 plantas de mangueira seriam suficientes, coletando-se uma folha nos quatro pontos cardeais da planta. Já para os micronutrientes, seriam necessárias, no mínimo, 20 plantas e, se considerarmos o Fe, seria necessário amostrar, pelo menos, 30 plantas.

Distribuição espacial de Oncometopia facialis (Signoret) (Hemiptera: Cicadellidae) em pomar cítrico

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Long-term facial artery catheter implantation for serial arterial blood sampling and invasive arterial blood pressure measurement in horses

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)