Quantitative cell-cycle protein expression in oral cancer assessed by computer-assisted system

The knowledge of cell-cycle control has shown that the capacity of malignant growth is acquired by the stepwise accumulation of defects in specific genes regulating cell growth. Histologic diagnosis might be improved by a quantitative evaluation of more specific diagnosis biomarkers, which could help to precisely identify pre-malignant and malignant oral lesions. The aim of the present study is to evaluate whether computer-based quantitative assessment of p53, PCNA and Ki-67 immunohistochemical expression, could be used clinically to foresee the risk of oral malignant transformation. This retrospective study was carried out in ninety-five oral biopsies, 27 were classified as fibrous inflammatory hyperplasia, 40 as leukoplakia and 28 as oral squamous cell carcinoma. Sixteen out of the 40 leukoplakia were diagnosed as non-dysplastic leukoplakia, the other 24 being dysplastic leukoplakia, of which 50.0% were classified as moderate to severe dysplasia. Comparison of the four groups of oral tissues showed significant rises in p53 and Ki-67 positivity index, which increased steadily in the order benign, pre-malignant, and malignant. In contrast, it was not possible to relate higher PCNA levels with pre-malignant and malignant oral lesions. We therefore conclude that PCNA immunohistochemistry expression is probably an inappropriate marker to identify oral carcinogenesis, whereas joint quantitative evaluation of p53 and Ki-67, appears to be useful as a tumor marker, providing a pre-diagnostic estimate of the potential for cell-cycle deregulation of the oral proliferate status.

Development of an open case-based decision-support system for diagnosis in oral pathology

Making diagnoses in oral pathology are often difficult and confusing in dental practice, especially for the lessexperienced dental student. One of the most promising areas in bioinformatics is computer-aided diagnosis, where a computer system is capable of imitating human reasoning ability and provides diagnoses with an accuracy approaching that of expert professionals. This type of system could be an alternative tool for assisting dental students to overcome the difficulties of the oral pathology learning process. This could allow students to define variables and information, important to improving the decision-making performance. However, no current open data management system has been integrated with an artificial intelligence system in a user-friendly environment. Such a system could also be used as an education tool to help students perform diagnoses. The aim of the present study was to develop and test an open case-based decisionsupport system.Methods: An open decision-support system based on Bayes' theorem connected to a relational database was developed using the C++ programming language. The software was tested in the computerisation of a surgical pathology service and in simulating the diagnosis of 43 known cases of oral bone disease. The simulation was performed after the system was initially filled with data from 401 cases of oral bone disease.Results: the system allowed the authors to construct and to manage a pathology database, and to simulate diagnoses using the variables from the database.Conclusion: Combining a relational database and an open decision-support system in the same user-friendly environment proved effective in simulating diagnoses based on information from an updated database.

A study on human error in the interaction with the computer systems

The term human factor is used by professionals of various fields meant for understanding the behavior of human beings at work. The human being, while developing a cooperative activity with a computer system, is subject to cause an undesirable situation in his/her task. This paper starts from the principle that human errors may be considered as a cause or factor contributing to a series of accidents and incidents in many diversified fields in which human beings interact with automated systems. We propose a simulator of performance in error with potentiality to assist the Human Computer Interaction (HCI) project manager in the construction of the critical systems. © 2011 Springer-Verlag.

Electromyographic validation of the double pulley equipment during movements of the lower limbs

The double pulley equipment was tested on ten male volunteers during contraction of the semitendinosus and biceps femoris (caput longum) muscles in the following movements of the lower limbs: 1) hip extension with extended knee and erect trunk, 2) hip extension with flexed knee and erect trunk, 3) hip extension with flexed knee and erect trunk, 3) hip extension with extended knee and inclined trunk, 5) hip abduction along the midline, 7) hip abduction with extension beyond the midline, 8) adduction with hip flexion beyond the midline, 8) adduction with hip flexion beyond the midline, and 9) adduction with hip extension beyond the midline. The myoelectric signals were taken up by Lec Tec surface electrodes connected to a 6-channel Lynx electromyographic signal amplifier coupled with a computer equipped with a model CAD 10/26 analogue digital conversion board and with a specific software for signal recording and analysis. The semitendinosus and biceps femoris muscles presented the highest potentials in movements 1; 2; 7, 8 and 9, whereas the potentials in the remaining movements were negligible. The pattern of activity of the semitendinosus and the biceps femoris was similar in exercises 1, 2, 3, 4 and 8. The potentials of the semitendinosus prevailed in movements 5, 6 and 7, and the strongest potentials observed in movement 9 were those of the biceps femoris.

Sistema inteligente para la selección de brocas de perforación de pozos

The present work begins with a review of the literature on bit selection methods for oil well drilling. A proposal for the structure and organization of a drilling database and a knowledge base, is described. Previous studies formed the principal elements in the process of selection of drills for proposed drilling. The procedure was implemented as a computer system for the selection of tricone bits. A drilling bit database for three different Brazilian sedimentary basins was obtained for several wells drilled, and knowledge was collected from drilling engineers from different fields both electronically and also by means of interviews. It can be concluded that the selection process showed good results based on tests, which were carried out.

Electromyographic analysis of the pectoralis major and deltoideus anterior muscles in horizontal flyer exercises with loads

The effective activity of the pectoralis major and deltoideus anterior muscles in horizontal flyer exercises with external loads of 25, 50, 75 and 100% of the maximum load was studied in 11 male volunteers. The electromyographic analysis was done by using MEDI-TRACE-200 surface electrodes connected to a biological signal acquisition mode coupled to a PC/AT computer. The electromyographic signals were processed and the values obtained were normalized through maximum voluntary isometric contraction. It was statistically observed that in all types and loads of this exercise, the muscles presented significant differences in the concentric and eccentric phases. In the concentric phase, when different loads were compared, the muscles were more active with 75 and 100% of the maximum load, while in the eccentric phase, higher activity was observed with 100% of the maximum load. By analyzing each load effect in the concentric phase, it was verified that the muscles on the left side were more active than those on the right side with 25, 75 and 100% of the maximum load.

Espacialização das estimativas de contaminação de águas subterrâneas por Tebuthiuron

Improper use of pesticides can lead to significant environmental impacts such as, contamination of environmental compartments, being the aquatic compartments the most vulnerable. In this context, the spatialization of pesticides concentrations estimative in groundwater provides important insights for decision making in managing and monitoring pesticides use. This study aimed to spatialize estimatives of groundwater contamination by Tebuthiuron, from different irrigation depths in the Rio Pardo basin, Pardinho-SP, Brazil. The simulations were performed using the ARAquá computer system, considering 0mm, 200 mm and 400 mm annual irrigation depths. Geostatistical techniques were used to obtain the spatial distribution of the simulated estimative. Tebuthiuron maps estimating concentration in groundwater were obtained by Kriging interpolation method, and indicated the areas with high potential for groundwater contamination. Considering all the simulations, it was concluded that there was no risk of groundwater contamination by Tebuthiuron in the study area.

Desenvolvimento de um sistema computacional para tratamento de dados meteorológicos no setor de agroenergia

Pós-graduação em Agronomia (Energia na Agricultura) - FCA

Sistema WEB para gerenciamento de informações agrícolas

Pós-graduação em Agronomia (Energia na Agricultura) - FCA

Espacialização das estimativas de contaminação de água subterrânea por defensivos agrícolas

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Desenvolvimento de um sistema computacional para monitoramento de parâmetros físicos na área de irrigação, com base na plataforma labVIEW

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência dos níveis protéicos fornecidos na dieta sobre o sistema reprodutivo de carneiros

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Nefropatia diabética experimental: estudo de parâmetros clínicos, laboratoriais e análise ultra-estrutural e morfométrica da membrana basal e podócitos glomerulares de ratos diabéticos induzidos pela aloxana

Pós-graduação em Bases Gerais da Cirurgia - FMB

Desenvolvimento de Pipelines para análise de EST, bibliotecas ITS e 16S para estudos genômicos em formigas Attini

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A tool for controlling accelerometers. Secondary calibration data

Vibration monitoring requires acceleration transducers capable of providing data with high precision. Accelerometers are the most frequently used vibration transducers. Their calibration plays an important role in measuring vibrations and is a key component in ensuring the integrity of the vibration measurement. For managing secondary calibration data of accelerometers, a database computer system was implemented. The implementation of this software has been an important step forward in providing a wide range of analysis and display tools. This paper reviews the main concepts involving accelerometer secondary calibration and describes the tool developed and the methods used in its development. (C) 2013 Academie des sciences. Published by Elsevier Masson SAS. All rights reserved.