Bud source, asepsis and benzylaminopurine (BAP) effect on yacon (Polymnia sonchifolia) micropropagation

The yacon (Polymnia sonchifolia) is used largely for the high fructan content of its tubers; consequently, it is a good alternative for diabetics. One of the more important restricting factors of the commercial production of yacon is its susceptibility to nematode attack. This, as well as germplasm bank maintenance, justifies the importance of in vitro propagation of this species. In this way, our work aimed to verify the best asepsis method for yacon for the in vitro establishment from the rhizophore and the axillary buds of the aerial parts, and the effect of benzylaminopurine (BAP) addition to the culture medium. The number of contaminated cultures, the occurrence of phenolic oxidation and the occurrence of a vitreous aspect, showed differences with bud source, immersion time for asepsis, and BAP use. The results contribute to establishing a yacon micro propagation procedure.

Effect of benzylaminopurine (BAP) on clonal propagation rate of Curcuma longa L.

Curcuma longa L. is used in many countries for its flavor, and medicinal and cosmetic attributes, as well as for its peculiar starch characteristics. These factors have driven an interest in the in vitro propagation of this species, looking for germplasm bank maintenance, production of disease free plants, genetic variability induction from callus, and as a tool for starch research. However, there are few reports concerning the micropropagation of Curcuma longa. The in vitro propagation rate of this species, cultured under two benzylaminopurine (BAP) concentrations, was the aim of this research.

Quality in anthurium andraeanum after postharvest BAP treatment

To ensure the ongoing quality of anthurium inflorescence it is necessary to use postharvest treatments aimed at extending the vase life and delaying senescence. Thus, the objective of this study was to assess the effect of postharvest spray application of 6-benzylaminopurine (BAP) in anthurium quality and sugar levels. Two experiments were done, one where inflorescences were sprayed 0, 100 and 200 mg L-1 of BAP and kept under 23±1°C and relative humidity of 65±5% for evaluations, and in the second experiment BAP concentrations were reduced to 0, 50 and 100 mg L-1 and inflorescences were cold stored for 2 days at 13±1°C and then transferred to the same evaluation conditions from the first experiment. Quality assessments were performed according to Paull (1982) for spathe loss of gloss and blueing and spadix necrosis along with stem weighting for fresh weight (FW) variation every two days. In the first experiment FW loss was significantly lower from the 14th until 26th day after harvest. Anthurium sprayed with 100 and 200 mg L-1 showed significantly less fresh weight loss when compared to control stems and in the second experiment 50 and 100 mg L-1 did not reduce fresh weight loss. Means of scores from quality analysis, blueing and loss of gloss of spathes and spadix necrosis, were significantly lower in BAP sprayed anthurium (100 and 200 mg L-1) than in control flowers and by lowering BAP spray concentration and cold storing stems for two days this positive effect was only observed for spadix necrosis and not observed for spathe parameters.

Cultura de embrião e indução de brotos in vitro para micropropagação do pinhão-manso

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Crescimento de plantas de Salvia officinalis sob ação de reguladores de crescimento vegetal

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Morphological and physiological responses of the recalcitrant Euterpe edulis seeds to light, temperature and gibberellins

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Rendimento de óleo essencial de Salvia officinalis L. sob ação de reguladores vegetais

This study aimed to evaluate the effect of different plant growth regulators on essential oil yield in Salvia officinalis L. plants. The experiment was held in a greenhouse and the experimental design was completely randomized, with 5 treatments and three replications. The Treatments consisted in the application of gibberellic acid (GA3), benzylaminopurine (BAP), 2-chloroethyl phosphonic acid (ethephon), and Stimulate at 2%, compared with control plants (water). Applications of plant growth regulators were performed in three consecutive periods, in turn, 15, 25 and 35 days after transplanting of seeding germinating in the light at 25°C. The dry mass yield of the aerial part and the oil essential content were determined 131 days after the transplant. The aerial part of the plants was dried in an oven at 35°C; after dry mass determination, the oil was extracted by hydrodistillation and its volume was determined. Plants treated with GA 3 and Stimulate showed increase in essential oil content, while plants treated with BAP and ethephon showed decrease in essential oil volume when compared with the control plants.

Micropropagação de Salvia officinalis L. com avaliação do teor de fenóis totais e atividade antioxidante

Stem cuts and seeds of Salvia officinalis were incubated on nutrient media for plantlets production and analysis of the total phenolic compounds, total flanovoids and antioxidant activity in micropropagated plants. Explants were obtained from seedlings and inoculated on MS with different concentrations of benzylaminopurine (BAP) and indolbutyric acid (IBA). After 30 and 60 days of incubation, the explants were scored for percentage of contaminated, dead and oxidized explants and mean bud numbers. For bud formation, the most efficient treatment was the medium containing BAP at 1 mg L-1, for the plantlet height the better medium was the control without the addition of plant regulator. IBA promoted the formation of few roots. Our results indicated that stem cuts incubated on media containing BAP and/or IBA did not increase the total flavonoid contents, but increased the total phenolics' by BAP and high antioxidant activity by 1 mg L-1 BAP.

Embriogênese somática e transformação genética de soja via biobalística

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Propagação de amoreira-preta (Rubus spp.) via brotação de estacas radiciais e enraizamento com a utilização de reguladores vegetais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Propagação de Hancornia speciosa Gomes – Apocynaceae, por alporquia e micropropagação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Reguladores vegetais e bioestimulantes no desenvolvimento de Salvia officinalis L: avaliações fisológicas, bioquímicas e fitoquímicas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produtos de efeitos fisiológicos no desenvolvimento de plantas de tomate ‘giuliana’, na produção e pós-colheita de frutos

Pós-graduação em Agronomia (Horticultura) - FCA

Protocolo para micropropagação de bananeira ‘Thap maeo’

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Métodos de propagação do pinhão manso (Jatropha curcas L.)

Pós-graduação em Agronomia - FEIS