Um sistema computacional para diagnosticar viroses de plantas usando a técnica de PCR com construção de primers espécie-específicos

It proposes a established computational solution in the development of a software to construct species-specific primers, used to improve the diagnosis of virus of plant for PCR. Primers are indispensable to PCR reaction, besides providing the specificity of the diagnosis. Primer is a synthetic, short, single stranded piece of DNA, used as a starter in PCR technique. It flanks the sequence desired to amplify. Species-specific primers indicate the well known region of beginning and ending where the polymerase enzyme is going to amplify on a certain species, i.e. it is specific for only a species. Thus, the main objective of this work is to automatize the process of choice of primers, optimizing the specificity of chosen primers by the traditional method

Avaliação do potencial fitorremediador da mamona (Ricinus communis L.) e girassol (Helianthus annuus L.) quanto à remoção de chumbo e tolueno em efluentes sintéticos.

The development of research that aim to reduce or even eliminate the environmental impacts provided by anthropogenic actions. One of these main action is the discard of industrial waste in the biotic compartments such as soil, water and air, gained more space in academic settings and in private. A technique of phytoremediation involving the use of plants (trees, shrubs, creepers and aquatic) and their associated microorganisms in order to remove, degrade or isolate toxic substances to the environment. This study aimed to evaluate the potential for phytoremediation of castor bean (Ricinus communis L.) and sunflower (Helianthus annuus L.), wild crops suitable region of Rio Grande do Norte, to reduce concentrations of lead and toluene present in synthetic wastewater that simulate the characteristics of treated water production originated in the petrochemical Guamaré. The experiment was accomplished in randomized blocks in four replicates. Seeds of BRS Energy for the development of seedlings of castor beans and sunflower for Catissol 01, both provided by EMPARN (Empresa de Pesquisa Agropecuária do Rio Grande do Norte) were used. Lead concentrations tested were 250, 500 and 1000 mg/L called T2, T3 and T4, respectively, for toluene the concentrations used were 125, 256 and 501 μg/L, called T5, T6 and T7, respectively. The data for removal of lead in relation to sewage systems applied in castor bean and sunflower were 43.89 and 51.85% (T2), 73.60 and 73.74% (T3) and 85.66 and 87.80 % (T4), respectively, and toluene were approximately 52.12 and 25.54% (T5), 55.10 and 58.05% (T6) and 79.77 and 74.76% (T7) for castor and sunflower seeds, respectively. From the data obtained, it can be deduce that mechanisms involved in reducing the contaminants were of phytoextraction, in relation to lead and phytodegradation for toluene. However, it can be concluded that the castor bean and sunflower crops can be used in exhaust after-treatment of industrial effluents that have this type of contaminant

Influência espaço-temporal e fisiológica na absorção de nutrientes e elementos tóxicos por Eichhornia crassipes visando o uso adequado da sua biomassa: o caso do Rio Apodi/Mossoró - RN

This study aims to determine the amount of nutrients and toxic elements in aquatic macrophytes of species Eichhornia crassipes present in River Apodi/Mossoró - RN and check some of the possibilities of using the biomass produced, based on the influence of space - temporal and physiological absorption of nutrients by plants. For this, was determined: Leaf area, Leaf wet mass, Leaf dry mass, Real humidity, Apparent humidity, Ash, Total nitrogen, Crude protein, Calcium, Magnesium, Potassium, Total phosphorus, Sodium, Iron, Copper, Manganese, Zinc, Nickel, Cobalt, Aluminum, Cadmium, Lead and Total chromium at different times, 2 sampling points and 2 parts of plants (leaves and roots). The results show that the levels of nutrients, protein and toxic elements present in plant tissue of Eichhornia crassipes are influenced by spatial, temporal and physiological variability. In general, because the maximum values in the dry matter for total nitrogen (4.4088 g/100g), crude protein (27.5549 g/100g), total phosphorus (0.642 g/100 g), calcium (1.444 g/100g), magnesium (0.732 g/100 g), potassium (7.51 g/100 g), copper (4.4279 mg/100g), manganese (322.668 mg/100g), sodium (1.39 g/100g), iron (194.169 mg/100g) and zinc (3.5836 mg/100g), there was the possibility of using biomass of Eichhornia crassipes for various purposes such as in food animal, products production for human consumption, organic fertilizers, fabrication of brick low cost, and crafts. For all these applications requires a control of the levels of substances in plant tissue. Based on the levels of nutrients and crude protein, the younger plants (0 Month) would be best to have their biomass used. Moreover, one factor that contributes to the use of larger plants (6 Months), the levels of toxic elements which have significantly small or below the detection limit. Therefore, further studies quantifying the biomass produced/m2 at 0 and 6 months are needed for a more correct choice for the best time of harvest

Métodos estatísticos recursivos aplicados ao problema de estimação de vazão de gás em plantas de Plunger-lift

This work has as main objective to find mathematical models based on linear parametric estimation techniques applied to the problem of calculating the grow of gas in oil wells. In particular we focus on achieving grow models applied to the case of wells that produce by plunger-lift technique on oil rigs, in which case, there are high peaks in the grow values that hinder their direct measurement by instruments. For this, we have developed estimators based on recursive least squares and make an analysis of statistical measures such as autocorrelation, cross-correlation, variogram and the cumulative periodogram, which are calculated recursively as data are obtained in real time from the plant in operation; the values obtained for these measures tell us how accurate the used model is and how it can be changed to better fit the measured values. The models have been tested in a pilot plant which emulates the process gas production in oil wells