Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats

Cyclophosphamide (CYP) is an antineoplastic agent used for the treatment of many neoplastic and inflammatory diseases. Hemorrhagic cystitis is a frequent side effect of CYP. Several studies show that simvastatin has important pleiotropic (anti-inflammatory and immunomodulatory) effects. The purpose of the study was to investigate the effect of simvastatin on bladder, ureter and kidney injury caused by CYP. Methods: Adult male Wistar rats were randomly divided into three groups. The CYP/SIM group received simvastatin microemulsion by gavage during 7 days (10 mg/kg body wt) before the administration of CYP and the CYP/SAL group rats received saline 0.9%. The control rats were not treated. After that, all rats were treated with a single dose of CYP 200 mg/kg body wt intraperitoneally. The rats were killed 24 h after CYP administration. Plasma cytokines (TNF-a, IL-1b, IL-6) were measured by ELISA. Macro and light microscopic study was performed in the bladder, kidney and ureter. Results: In the bladders of CYP/SIMV treated rats edema of lamina propria with epithelial and sub-epithelial hemorrhage were lower than in CYP/SAL treated rats. The scores for macroscopic and microscopic evaluation of bladder and ureter were significantly lower in CYP/SIMV rats than in CYP/SAL rats. The kidney was not affected. The expression of TNF-a, IL-1b and IL-6 was significatly lower in CF/SINV rats (164.8±22, 44.8±8 and 52.4±13) than in CF/SAL rats (378.5±66, 122.9±26 e 123.6±18), respectively. Conclusion: The results of the current study suggest that simvastatin pretreatment attenuated CYP-induced urotelium inflammation and decreased the activities of cytokines

A importância do holding na reorganização afetivo-social de crianças que manifestam tendência anti-social

This study aims to understand as the children who show expressive behaviors of antisocial trends at Núcleo de Ação Social da Praia do Meio in Natal-RN city, linked to Secretaria Municipal do Trabalho e Assistência Social-SEMIT, which experience the relation with the social educator in role of caregiver. This matter was investigated within an interpretation and approach qualitative view of the social phenomena, using the Thematic Analysis technique of drawings and stories which express the relation with the caregiver gotten in sessions of playful interactions between child and researcher in order to understand the child experiences with the social educator. The used theoretical referential is by D. W. Winnicott who associates the social trend to a failure of environment in child holding. The subjects expressed the need of a firm and protective environment and they look for this in NASPM educators. Moreover, they show as solitude and vulnerability take part their lives due to the lack of emotional holding on family that favors the involvement with violent groups. But, it is worth pointing out that the children value the Nucleus environment when has offered them a safe holding which can be positive the established linking with it. Such conclusion points out to the possibility, recognized theoretically, that exists from these children to resume the way of healthy development