Isolamento de uma quitinase extraída do cefalotórax do camarão marinho Litopenaeus schmitti (BURKENROAD, 1936) : avaliação de suas atividades antimicrobiana e larvicida

Chitinases are enzymes involved in degradation of chitin and are present in a range of organisms, including those that do not contain chitin, such as bacteria, viruses, plants and animals, and play important physiological and ecological roles. Chitin is hydrolyzed by a chitinolytic system classified as: endo-chitinases, exo-chitinases and N-acetyl-b-D-glucosaminidases. In this study a Litochitinase1 extracted from the cephalotorax of the shrimp Litopenaeus Schmitt was purified 987.32 times using ionexchange chromatography DEAE-Biogel and molecular exclusion Sephacryl S-200. These enzyme presented a molecular mass of about 28.5 kDa. The results, after kinetic assay with the Litochitinase1 using as substrate p-nitrophenyl-N-acetyl-b-Dglucosaminideo, showed apparent Km of 0.51 mM, optimal activity at pH ranging from 5.0 to 6.0, optimum temperature at 55°C and stability when pre-incubated at temperatures of 25, 37, 45, 50 and 55°C. The enzyme showed a range of stability at pH 4.0 to 5.5. HgCl2 inhibited Litochitinase1 while MgCl2 enhances its activity. Antimicrobial tests showed that Litochitinase1 present activity against gram-negative bacterium Escherichia coli in the 800 μg/mL concentration. The larvicidal activity against Aedes aegypti was investigated using crude extracts, F-III (50-80%) and Litochitinase1 at 24 and 48 hours. The results showed larvicidal activity in all these samples with EC50 values of 6.59 mg/mL for crude extract, 5.36 mg/mL for F-III and 0.71 mg/mL for Litochitinase1 at 24 hours and 3.22 and 0.49 mg/mL for the F-III and Litochitinase1 at 48 hours, respectively. Other experiments confirmed the presence of chitin in the midgut of Aedes aegypti larvae, which may be suffering the action of Litochitinase1 killing the larvae, but also the absence of contaminating proteins as serine proteinase inhibitors and lectins in the crude extract, F-III and Litochitinase1, indicating that the death of the larvae is by action of the Litochitinase1. We also observed that the enzymes extracted from intestinal homogenate of the larvae no have activity on Litochitinase1. These results indicate that the enzyme can be used as an alternative to control of infections caused by Escherichia coli and reducing the infestation of the mosquito vector of dengue.

Construção, caracterização e aplicação de eletrodos para descarga por arco de cátodo oco

The system built to characterize electrodes and, consequently, deposited fine films are constituted by a hollow cathode that works to discharges and low pressures (approximately 10-3 to 5 mbar), a source DC (0 to 1200 V), a cylindrical camera of closed borossilicato for flanges of stainless steel with an association of vacuum bombs mechanical and spread. In the upper flange it is connected the system of hollow cathode, which possesses an entrance of gas and two entrances for its refrigeration, the same is electrically isolated of the rest of the equipment and it is polarized negatively. In front of the system of hollow cathode there is a movable sample in stainless steel with possibility of moving in the horizontal and vertical. In the vertical, the sample can vary its distance between 0 and 70 mm and, in the horizontal, can leave completely from the front of the hollow cathode. The sample and also the cathode hollow are equipped with cromel-alumel termopares with simultaneous reading of the temperatures during the time of treatment. In this work copper electrodes, bronze, titanium, iron, stainless steel, powder of titanium, powder of titanium and silício, glass and ceramic were used. The electrodes were investigated relating their geometry change and behavior of the plasma of the cavity of hollow cathode and channel of the gas. As the cavity of hollow cathode, the analyzed aspects were the diameter and depth. With the channel of the gas, we verified the diameter. In the two situations, we investigated parameters as flow of the gas, pressure, current and applied tension in the electrode, temperature, loss of mass of the electrode with relationship at the time of use. The flow of gas investigated in the electrodes it was fastened in a work strip from 15 to 6 sccm, the constant pressure of work was among 2.7 to 8 x 10-2 mbar. The applied current was among a strip of work from 0,8 to 0,4 A, and their respective tensions were in a strip from 400 to 220 V. Fixing the value of the current, it was possible to lift the curve of the behavior of the tension with the time of use. That curves esteem in that time of use of the electrode to its efficiency is maximum. The temperatures of the electrodes were in the dependence of that curves showing a maximum temperature when the tension was maximum, yet the measured temperatures in the samples showed to be sensitive the variation of the temperature in the electrodes. An accompaniment of the loss of mass of the electrode relating to its time of use showed that the electrodes that appeared the spherical cavities lost more mass in comparison with the electrodes in that didn't appear. That phenomenon is only seen for pressures of 10-2 mbar, in these conditions a plasma column is formed inside of the channel of the gas and in certain points it is concentrated in form of spheres. Those spherical cavities develop inside of the channel of the gas spreading during the whole extension of the channel of the gas. The used electrodes were cut after they could not be more used, however among those electrodes, films that were deposited in alternate times and the electrodes that were used to deposit films in same times, those films were deposited in the glass substrata, alumina, stainless steel 420, stainless steel 316, silício and steel M2. As the eletros used to deposit films in alternate time as the ones that they were used to deposit in same times, the behavior of the thickness of the film obeyed the curve of the tension with relationship the time of use of the electrode, that is, when the tension was maximum, the thickness of the film was also maximum and when the tension was minimum, the thickness was minimum and in the case where the value of the tension was constant, the thickness of the film tends to be constant. The fine films that were produced they had applications with nano stick, bio-compatibility, cellular growth, inhibition of bacterias, cut tool, metallic leagues, brasagem, pineapple fiber and ornamental. In those films it was investigated the thickness, the adherence and the uniformity characterized by sweeping electronic microscopy. Another technique developed to assist the production and characterization of the films produced in that work was the caloteste. It uses a sphere and abrasive to mark the sample with a cap impression, with that cap form it is possible to calculate the thickness of the film. Through the time of life of the cathode, it was possible to evaluate the rate of waste of its material for the different work conditions. Values of waste rate up to 3,2 x 10-6 g/s were verified. For a distance of the substratum of 11 mm, the deposited film was limited to a circular area of 22 mm diameter mm for high pressures and a circular area of 75 mm for pressure strip. The obtained films presented thickness around 2,1 µm, showing that the discharge of arch of hollow cathode in argon obeys a curve characteristic of the tension with the time of life of the eletrodo. The deposition rate obtained in this system it is of approximately 0,18 µm/min

O som do silêncio :a angústia social que encobre o luto : um estudo sobre isolamento e sociabilidade entre enlutados do cemitério Morada da Paz (Natal/RN)

Nowadays, the act of mourning configures as a circumstance of isolation and social anguish due to the impossibility to express the pain by those who suffer the loss of a dear one. At the same time, the symbolic relations established between society and the cemetery contains interpretations about death, pain and fear which confer to the visits a special and individual feature. In this context, this dissertation tries to analyse the formation of a sociability observed among visitors of the Morada da Paz a private cemetery located in Natal/RN as well as the frequency of the visitors to the necropolis. Therefore, the research was accomplished from the observation of the existent relations among these actors whose meetings have procured links based on the experiences´ exchange about pain and bereavement. In this sense, this study tries to analyse, as its principal point, in which way the Morada da Paz space and the relations established there are being appropriate by the visitors in their mourning process, trying to understand what are the role of these bond in the elaboration of the loss for these people

Fazer a ponte para a escola de todos(as)

The purpose of this study is to understand what are the matrix elements in which everyday activities of social author/authors, actor/actors and actress/actresses are embedded, in order to carry out the Escola da Ponte s political and pedagogic process (PPP). Thus, the object of this study is to reflect on an experience developed in a public school supported by the Portuguese State within the scenario of institutions that are committed to building up a school of the people by investing in democratization and community participation in their management process. The methodological course of action was targeted at the presuppositions of qualitative research (WOODS, 1999; BURGESS, 1997; MILLS, 1982; OLIVEIRA, 1998; BOGDAN and BIKLEN, 1994; MACEDO, 2000; CASAL, 1996; GEERTZ, 2000), with data collected from random word association (MACHADO and CARVALHO, 2003), the discourse of the interviewed (KAUFMANN, 1996; SILVA, 2006; 2002), observation notes and documents (ELIAS, 1997). Based on a multiple reference and theoretical framework, the investigation revealed elements that are construed as the raw material and pillars supporting the bridge of a school of the people, as well as the self-organization of the school community which emerges as a dimension that binds together all the elements and brings new arrangements into the school dynamics by moving from the individual to the society (DUPUY, 1990). Along this line, the central argument is that in the short and long run the public school quality does not spring from macro educational policies, nor from ephemeral mass-production programs, projects or governmental policies, but from the intent of the professionals working there in becoming authors and characters in the process of building up and carrying out the PPP. The Project has been conceived in the light of the needs and intentionality of the community whose support comes from the participation and social control held by the students families and the community s power to force to action public administration central agencies aiming at having the State fulfill its responsibilities

A construção da ponte metálica sobre o Rio Pontengi: aspectos históricos, construtivos e de durabilidade - Natal/RN, Brasil (1912-1916) - estudo de caso

It is a case study that reports the construction of metal truss bridge in the river Potengi in Natal, Rio Grande do Norte, between the years 1912 and 1916. From testimonials on steel bridges in Brazil and worldwide including foundations. Documentary research from procurement of projects and contracts was performed. A chronology of construction, with a description of the equipment used and its original budget with the Brazilian government. Still, we used interviews and surveys with experimental sampling / testimonies, laboratory tests. This study aims to analyze historically and technically the Bridge over the River Potengi, emphasizing primarily the construction process, the qualities and characteristics of the materials used and the technological, chemical, mineralogical and microstructural properties of cement and concrete used in its construction. Taking as conclusions that cements pozolônicos ensured a good durability to the concrete in a hundred-year period and that the solution employed with the compressed air caissons was right

Sistema de detecção e isolamento de falhas em sistemas dinâmicos baseado em identificação paramétrica

The present research aims at contributing to the area of detection and diagnosis of failure through the proposal of a new system architecture of detection and isolation of failures (FDI, Fault Detection and Isolation). The proposed architecture presents innovations related to the way the physical values monitored are linked to the FDI system and, as a consequence, the way the failures are detected, isolated and classified. A search for mathematical tools able to satisfy the objectives of the proposed architecture has pointed at the use of the Kalman Filter and its derivatives EKF (Extended Kalman Filter) and UKF (Unscented Kalman Filter). The use of the first one is efficient when the monitored process presents a linear relation among its physical values to be monitored and its out-put. The other two are proficient in case this dynamics is no-linear. After that, a short comparative of features and abilities in the context of failure detection concludes that the UFK system is a better alternative than the EKF one to compose the architecture of the FDI system proposed in case of processes of no-linear dynamics. The results shown in the end of the research refer to the linear and no-linear industrial processes. The efficiency of the proposed architecture may be observed since it has been applied to simulated and real processes. To conclude, the contributions of this thesis are found in the end of the text

Detecção e isolamento de falhas em sistemas dinâmicos baseados em redes neurais

This master dissertation presents the development of a fault detection and isolation system based in neural network. The system is composed of two parts: an identification subsystem and a classification subsystem. Both of the subsystems use neural network techniques with multilayer perceptron training algorithm. Two approaches for identifica-tion stage were analyzed. The fault classifier uses only residue signals from the identification subsystem. To validate the proposal we have done simulation and real experiments in a level system with two water reservoirs. Several faults were generated above this plant and the proposed fault detection system presented very acceptable behavior. In the end of this work we highlight the main difficulties found in real tests that do not exist when it works only with simulation environments

Desenvolvimento de material compósito a base de raspa de pneu e látex para isolamento térmico

The process of recycling has been stimulated by the markets for several reasons, mainly on economical and environmental. Several products have been developed from recycled materials that already exist as well as several residues have been studied in different forms of applications. The greater majority of the applications for thermal insulation in the domestic, commercial and industrial systems have been elaborated in the temperature ranges between low to medium reaching up to 180oC. Many materials such as glass wool, rock wool, polystyrene are being used which are aggressive to the environment. Such materials in spite of the effectiveness in the retention of heat flow, they cost more and when discarded take several years to be absorbed by the nature. This way, in order to adapt to a world politics concerning the preservation of the environment, the present study was intended to develop a material composed of natural/biodegradable materials and industrial residues. The development of such a product in the form of a composite material based on tyre scrapes and latex for thermal insulation is presented in this research work. Thermal and physical properties of the tire scrapes as well as latex were studied in order to use them as raw materials for the manufacture of the intended composite to be applied as a thermal insulator in hot and cold systems varying between 0ºC and 200oC, respectively. Composite blankets were manufactured manually, in weight proportions of 1:1 (50:50%); 1:2 (33:67%) and 2:1 (67:33%) (tire scrapes: latex) respectively. Physical, mechanical and thermal properties of the composites were analyzed to obtain data about the viability of using the composite as a thermal insulator. The analyses carried out were based on standards ABNT, ASTM and UL. The maximum temperature obtained for the composite as a thermal insulator was 200ºC, which meets the range of applications that could be used as a thermal insulator in domestic as well as industrial purposes. The experimental results prove that the composite can be used as a thermal insulator on heated or cooled surface

As Filhas do Arco-Íris, de Eulício Farias de Lacerda : mitos, lendas e contos populares como elementos estruturantes do romance

The corpus of this work constitutes an analysis the literary work As Filhas do Arco-Íris, by Eulício Farias de Lacerda, considering object of study myths, legends and popular stories as estruturant elements of the romance. The unchainment of this reading considers aspects of the oral tradition as starting point for the rupture tradition. In that perspective, it became fundamental to describe aspects of the popular culture that consolidated the Brazilian Literature during the 20th century. Like this, it was necessary to understand the development of oral elements and the structuring of the folk-tales, pointing out that As Filhas do Arco-Íris was built in a especial way with regionalist characteristics, following the parameters of the romances published among the years 1930 and 1950. In the researched work, it was noticed a narrative that gathers stories, legends, myths, proverbs and oral locutions. In this, it reveals that the Brazilian literary production grew in aesthetics and thematic. Like this, As Filhas do Arco-Íris is an exercise of the experience regionalist inserted in the contemporary novel, because even with your first publication in 1980, the narrative is configured to the molds of a Romance de 30

Experiência, memória e humor: representações sociais em as filhas do arco-íris e em primeiras estórias.

Este trabalho analisa relações da experiência, memória e humor e investiga representações da infância, da velhice, da loucura e do alcoolismo no romance As Filhas do Arco-Íris, de Eulício Farias de Lacerda e em contos de Primeiras Estórias, de Guimarães Rosa, destacando-se que meninos, velhos, cegos e loucos, mesmo marginalizados, podem ser encarados como atores sociais na formação das comunidades. Verifica-se que essas personagens constituem uma coletividade no sentido de evocar a representação de indivíduos. Também se procura examinar práticas literárias e culturais no âmbito do sistema literário brasileiro, com ênfase em regionalismos, oralidade e considerações sobre literatura e sociedade. É desse modo que se busca observar que as obras destacadas estão inseridas numa discussão moderna acerca do desenvolvimento sociocultural. Portanto, a preocupação desta pesquisa deriva da discussão moderna acerca do desenvolvimento social. Diante disso, observa-se nos textos de Guimarães Rosa e de Eulício Farias um tratamento narrativo em torno dessas personagens, enfatizando que as situações vividas por eles envolvem experiências que os levam a construir, em relação ao contexto social, aspectos de amadurecimento, rememoração, compartilhamento, encantamento, viagem ao desconhecido, movimentos históricos, alegrias e tristezas, sempre demonstrando uma sintonia com a temática da modernidade. Assim, as condições sociais são interpretadas para evidenciar e confrontar: discriminação, amizade, conflito, respeito, marginalização, memória social. Ainda são estabelecidas comparações entre personagens do romance As Filhas do Arco-Íris com as de alguns contos de Primeiras Estórias, salientando-se considerações sobre o narrador, as inter-relações, regionalismo e relações entre literatura, organização e processo social. Esta pesquisa tem por base o conceito de sistema literário consolidado idealizado por Antonio Candido e os postulados de estudiosos e teóricos como: Walter Benjamin, Jacqueline Held, Mikhail Bakhtin, Roland Barthes, Ana Paula Pacheco, Serge Moscovici, François Laplantine, Liane Trindade

Modelo de ashkin-teller de três cores na rede ponte de wheatstone

In this work we study the phase transitions of the ferromagnetic three-color Ashkin-Teller Model in the hierarquical lattice generated by the Wheatstone bridge using real space renormalization group approach. With such technique we obtain the phase diagram and its critical points with respective critical exponents v. This model presents four phases: ferromagnetic, paramagnetic and two intermediates. Nine critical points were found, three of which are of Ising model type, three are of four states Potts model type, one is of eight states Potts model type and the last two which do not correspond to any Potts model with integer number of states. iv

Qualidade de vida, eficiência mastigatória e presença de disfunção temporomandibular em usuários de prótese parcial removível com arco dental curto inferior

Even nowadays there is in Brasil a large number of edentulous and a precarious financial condition of most of the population. In addition, World Health Organization aims for oral health, which consists on the maintenance of a natural dentition, functional and aesthetic composed of at least 20 teeth, without need of prosthetic intervention throughout life. From this and considering the lack of researches about the permanence of edentulous spaces in the oral cavity, and also avoiding overtreatment, this research has been proposed. Thus, the aim was to evaluate the effect of different lengths of the shortened lower dental arch in the presence or absence of a removable partial denture (RPD) on masticatory function, quality of life and occurrence of temporomandibular dysfunction. To achieve this goal, we compared the masticatory efficiency (colorimetric test), the oral comfort through the analysis of the impact of oral health in quality of life (OHIP-14), the presence of temporomandibular dysfunction (RDC/TMD) and the general quality of life (WHOQOL) of patients with shortened dental arches (SDA) (n=60), which is an arch with a reduction of teeth starting posteriorly, and patients with complete dental arch (Complete DA) (n = 34). The group of patients whit SDA was divided among PPR wears (PPD + SDA) (n = 17) and non-wears (n = 43). The population of this study consisted on patients who received or looked for treatment at the clinics of the Department of Dentistry of UFRN, from clinical analysis and records. The sample was chosen by convenience. For statistical analysis, it was a database in SPSS 17.0, followed by descriptive analysis with frequencies, absolute values, tests of central tendency and variability. The statistical tests used were chi-squared and analysis of variance as well as Tukey s post test, when applicable, all with a 95% confidence level. The results shown a prevalence of TMD of 47,1% among patients using PRP and 69,8% among those who didn t, but this result wasn t statistically significant. The mean of the results of masticatory efficiency, WHOQOL and OHIP didn t show association to the presence or absence of PPR and to the lower number of occlusal units of the patients (0, 1, 2 or more occlusal). The association only occurred among the different groups of SDA and the patients with complete dental arch. Taking into account the results, it could be observed that studied patients with low posterior support using lower PRP didn t have better masticatory efficiency, general quality of life, less impacts of their oral conditions in quality of life or not even less temporomandibular dysfunction or better masticatory efficiency when compared to those who didn t use the prosthesis

Aplicação de resíduo industrial para isolamento térmico: uma proposta para utilização do poliuretano de mamona com agregado de resíduo plástico termofixo

A aplicação de isolantes térmicos em sistemas construtivos promove vantagens com vistas ao conforto térmico de ambientes. E com isso, o aumento da produtividade em locais de trabalho, a sensação de bem-estar e a diminuição dos custos com climatização. A demanda por conforto ambiental, no âmbito da isolação térmica, somada ao advento de novas leis que regulam os requisitos mínimos de conforto, as exigências dos consumidores pela adoção de métodos de produção mais “limpos”, a fiscalização quanto à destinação de resíduos industriais, além da inserção de produtos no mercado com apelos ambientais, incentivaram o desenvolvimento da presente pesquisa. O presente trabalho trata da aplicação do poliuretano, visando comparar o desempenho térmico do derivado de origem vegetal (óleo de mamona) com adição de resíduo plástico termofixo em diferentes proporções (5%, 10%, 15% e 20%), com o poliuretano petrolífero, a lã de vidro e a lã de rocha através da análise de suas propriedades térmicas (condutividade térmica – k, difusividade térmica – e capacidade calorífica – Cp). . Após a realização dos ensaios, os compósitos estudados foram moídos e reutilizados como carga para novos compósitos. Com base nos resultados dos ensaios de propriedades térmicas, constatou-se que o material desenvolvido conduz menos calor que o poliuretano de petróleo, a lã de vidro e a lã de rocha, além de oferecer alta inércia térmica, bom desempenho térmico e baixo custo. Assim como foi comprovada a possibilidade de fabricação de novos compósitos para fins de isolamento, reutilizando os compósitos testados

Os marcos da paisagem da cidade no contexto da trama urbana de Natal/RN: um estudo a partir do Morro do Careca, o Midway Mall, o Pórtico dos Reis Magos e a Ponte Newton Navarro

The present work is a study on the functions performed by landmarks landscape Natal / RN in the urban fabric, in the case of Midway Mall, the Portico dos Reis Magos, the Morro do Careca and Newton Navarro Bridge. For such goals established itself as analyze the functionality of the milestones presented here in the urban fabric; discuss the importance of these landmarks of the city landscape in the process of urbanization of Natal, and identify and spatialize actions related to the use of territories where are located landmarks, discussing their reflections. To achieve these goals we chose to use as a literature search procedures, the problematic concept, working with oral sources, and reading speechless. To support this research the main concepts were worked landscape, city, landmark, public space, circuits, among others. After going over the theory, consistent with the empirical data, it appears that the landmarks studied here have different functions and features, but all carry different forms of fascination for the public and visitors to the city, and every man your way, influence the public space, land speculation and feature importance in the process of urbanization of Natal

Obtenção e caracterização de um eco-compósito à base de látex e bainha da palha do coqueiro para isolamento térmico e acústico

With the emergence of new technologies, has grown the need to use new materials, and this has intensified research on the collection and use of materials from renewable sources, is to reduce production costs and / or environmental impact. In this context, it was found that the sheath coconut straw, can be utilized as raw material for the production of a eco-composite that can be used as a thermal and acoustic insulator. After selected from the coconut sheaths were subjected to treatment with aqueous 2 % sodium hydroxide (NaOH). The composite study was produced with the sheath and coconut natural latex, with coconut sheath percentage in the proportions 15%, 25% and 35% of the total compound volume. Physical, thermal and acoustic properties of the composites were analyzed in order to obtain data on the use of viability as thermoacoustic insulation. The CP15 composites, CP25 and CP35 showed thermal conductivity 0.188 W/m.K, 0.155 W/m.K and 0.150 W/m.K, respectively. It can be applied as thermal insulation in hot systems to 200 ° C. The CP35 composite was more efficient as a thermal and acoustic insulation, providing 20% noise reduction, 31% and 34% for frequencies of 1 kHz, 2 kHz and 4 kHz, respectively. The analyzes were based on ABNT, ASTM, UL. Based on these results, it can be concluded that the eco-composite produced the hem of coconut can be used as thermal and acoustic insulation. Thus, it gives a more noble end to this material, which most often is burned or disposed of improperly in the environment.