Efeito do tratamento por plasma na proliferação de fibroblastos e esterilização de membranas de quitosana

Chitosan is being studied for use as dressing due their biological properties. Aiming to expand the use in biomedical applications, chitosan membranes were modified by plasma using the following gases: nitrogen (N2), methane (CH4), argon (Ar), oxygen (O2) and hydrogen (H2). The samples were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), contact angle, surface energy and water absorption test. Biological Tests were also performed, such as: test sterilization and proliferation of fibroblasts (3T3 line). Through SEM we observed morphological changes occurring during the plasma treatment, the formation of micro and nano-sized valleys. MFA was used to analyze different roughness parameters (Ra, Rp, Rz) and surface topography. It was found that the treated samples had an increase in surface roughness and sharp peaks. Methane plasma treatment decreased the hydrophilicity of the membranes and also the rate of water absorption, while the other treatments turned the membranes hydrophilic. The sterilization was effective in all treatment times with the following gases: Ar, N2 and H2. With respect to proliferation, all treatments showed an improvement in cell proliferation increased in a range 150% to 250% compared to untreated membrane. The highlights were the treatments with Ar 60 min, O2 60 min, CH4 15 min. Observing the results of the analyzes performed in this study, it appears that there is no single parameter that influences cell proliferation, but rather a set of ideal conditions that favor cell proliferation

Caracterização de superfícies de titânio modificado por oxidação à plasma

Recent years have seen a significant growth in surface modifications in titanium implants, resulting in shorter healing times in regions with low bone density. Among the different techniques, subtraction by chemical agents to increase oxidation has been applied for surface treatment of dental implants. However, this technique is generally unable to remove undesirable oxides, formed spontaneously during machining of titanium parts, raising costs due to additional decontamination stages. In order to solve this problem, the present study used plasma as an energy source to both remove these oxides and oxidize the titanium surface. In this respect, Ti disks were treated by hollow cathode discharge, using a variable DC power supply and vacuum system. Samples were previously submitted to a cleaning process using an atmosphere of Ar, H2 and a mixture of both, for 20 and 60 min. The most efficient cleaning condition was used for oxidation in a mixture of argon (60%) and oxygen (40%) until reaching a pressure of 2.2 mbar for 60 min at 500°C. Surfaces were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), atomic force microscopy (AFM), adhesion and cell proliferation. SEM showed less cell spreading and a larger number of projections orfilopodia in the treated samples compared to the control sample. AFM revealed surface defects in the treated samples, with varied geometry between peaks and valleys. Biological assays showed no significant difference in cell adhesion between treated surfaces and the control. With respect to cell proliferation, the treated surface exhibited improved performance when compared to the control sample. We concluded that the process was efficient in removing primary oxides as well as in oxidizing titanium surfaces

Análise topológica experimental por perfilometria cíclica indutiva de superficies de paredes estruturais

To enhance the maintenance practices, Oil and Gas Pipelines are inspected from the inside by automated systems called PIG (Pipeline Inspection Gauge). The inspection and mapping of defects, as dents and holes, in the internal wall of these pipelines are increasingly put into service toward an overall Structural Integrity Policy. The residual life of these structures must be determined such that minimize its probability of failure. For this reason, the investigation on the detection limits of some basic topological features constituted by peaks or valleys disposed along a smooth surface is of great value for determining the sensitivity of the measurements of defects from some combinations of circumferential, axial and radial extent. In this investigation, it was analyzed an inductive profilometric sensor to scan three races, radius r1, r2, r3, in a circular surface of low carbon steel, equipped with eight consecutive defects simulated by bulges and holes by orbit, equally spaced at p/4 rad. A test rig and a methodology for testing in laboratory were developed to evaluate the sensor response and identify their dead zones and jumps due to fluctuations as a function of topological features and scanning velocity, four speeds different. The results are presented, analyzed and suggestions are made toward a new conception of sensor topologies, more sensible to detect these type of damage morphologies

Os caminhos da transição agroecológica: uma análise das experiências da agricultura familiar camponesa no território dos Vales do Curu e aracatiaçu-CE

This study examines peasant family farming from an agroecological perspective. It intends to analyze the changes resulting from the transition from conventional to agro-ecological agriculture in the daily practices of farmers articulated associated with the Network of Agroecological and Solidarity Farmers of the Curu and Aracatiaçu Valleys Territory, the locus of this empirical research, and a space which has highlighted the social dynamics of agroecological innovation, as well as articulating environmental exchanges and knowledge development. As a way to further that goal, we seek to identify the forms of social organization previously present in the daily lives of these subjects, in addition to grasping the determinants that lead or led them to adopt agroecology, noting the need to verify the forms of resistance, and the strategies adopted by farmers and how they articulate collectively. Through the historical and dialectical methods, we seek to take the implications of technical modernization of agriculture under the conditions of production and reproduction of peasants and thus situate the emergence of agroecology, a focus that is born as a counterpoint to conventional patterns of agricultural development based on the paradigm of the Green Revolution. We structured this study around the trajectory of agroecological farmers that developed and internalized agroecological practices, processes, and organizational forms. For the analysis, we used theoretical and methodological frameworks from literature related to field research. The systematization and analysis of experiments revealed that agroecological transition is a broad process of change, not restricted to technical matters. We observed changes in production practices, diversification of production and feeding practices, ecological awareness, production autonomy, and organizations formed to face the challenges resulting from the imposition of the dominant agricultural development model that combines environmental degradation, land ownership concentration, and wealth concentration

Geomorfologia e neotectônica no Vale do Apodi-Mossoró RN

Numerous studies have indicated that the Potiguar Basin is affected by Cenozoic tectonics. The reactivation of Cretaceous fault systems affect the post-rift units, witch include Neogene and overlying Quaternary sediments. In this context, the objectives of this thesis are the followings: (1) to characterize the effects of post-rift tectonics in the morphology of Apodi Mossoró-river valley located in the central portion of the Potiguar, (2) to characterize the drainage of the Apodi Mossoró river valley and investigate the behavior of their channels across active faults, and (3) to propose a geologic-geomorphological evolutionary model for the study area. This study used a geological and geomorphological mapping of the central part of the basin, with emphasis on the Quaternary record, luminescence dating of sediments, and geoelectric profiles of the area. The results reveal by maps of structural lineaments and drainage channels of the rivers form valleys that are affected by faults and folds. In Apodi-Mossoró valley, anomalies of channel morphology are associated with the deformation of the post-rift basin. These anomalies show the reactivation of major fault systems in the Potiguar Basin in Cenozoic. On a regional scale, can be seen through the vertical electric profiles that the Cenozoic tectonics is responsible for the elevation of a macro dome NE-SE-trending 70-km long and 50km wide and up to 270 above sea level. In this sector, the vertical electric profiles data show that the contact between the Cretaceous and Neogene rise more than 100m. This Is an important feature of inversion data obtained in this work showed that the deposits that cover the macro dome (Serra do Mel) have ages of 119 ka to 43 ka. In the river valley and surrounding areas Apodi-Mossoró ages vary between 319 ka and 2.7 ka. From these data it was possible to establish the correct geochronological posiconamento paleodepósitos of distinguishing them from the fluvial deposits of the Neogene (Barreiras Formation)

Caracterização geomorfológica e paleogeográfica da plataforma continental adjacente a foz do Rio Apodi-Mossoró, RN/Brasil

The brazilian marginal basins have a huge potential to generate and accumulate petroleum. Incised valleys which are eroded in response to a fall of relative sea level are related to potential reservoir as well, modern drowned-valley estuaries serve as harbors to petroleum and salt industries, fisheries, waste-disposal sites and recreational areas for a significant fraction of the world s population. The combined influence of these factors has produced a dramatic increase in research on modern and ancient incised-valley systems. This research is one expression of this interest. The integrated use of satellites images and high resolution seismic (bathymetry, sides scan sonar) was used on the Apodi River mouth-RN to characterizes the continental shelf This area is located at the Potiguar Basin in the NE Brazilian Equatorial Atlantic margin. Through bathymetric and side scan sonar data processing, a digital Terrain Model was developed, and a detailed geomorphologic analysis was performed. In this way was possible to recognize the geomorphologic framework and differents sismofacies, which may influence this area. A channel extending from the ApodiMossoró river mouth to the shelf edge dominates the investigated area. This structure can be correlated with the former river valley developed during the late Pleistocene sea level fall. This channel has two main directions (NW-SE and NE-SW) probably controlled by the Potiguar Basin structures. The western margin of the channel is relatively steep and pronounced whereas the eastern margin consists only of a gentle slope. Longitudinal bedforms and massive ridges also occur. The first are formed doe to the shelf sediment rework and the reef-like structures probably are relics of submerged beachrock-lines indicating past shoreline positions during the deglacial sea-level rise. The sub-bottom seismic data allow the identification of different sismic patterns and a marcant discontinuity, interpreted as the Upper

Caracterização morfológica-sedimentar do vale inciso Apodi-Mossoró e plataforma continental adjacente - Bacia Potiguar Offshore

Systems of incised valleys have been studied in different continental shelves, including the Brazilian continental margin. The interest to characterize this feature is given by the information that it can provide variations on sea level, as well as the ability to host economically significant quantities of hydrocarbons in reservoirs located in deposits filling of the incised valleys. This thesis has the overall objective to characterize the morphology and sedimentary cover of the incised valley Apodi-Mossoró, located in the Northern Continental shelf of Rio Grande do Norte state, adjacent to Areia Branca city. The methodology included the integration of satellite imagery, bathymetric data, sedimentological data, shallow seismic, and the identification of foraminifera. The results indicate that the ApodiMossró incised valley is currently formed by two channels, shallow channel and deep channel, which have distinct morphological and sedimentological characteristics. The deep channel has connection with one of the heads of the Apodi Canyon, located in the slope area. The acquisition, processing and interpretation of shallow seismic data allowed the recognition of the depositional surface, erosional surface, discordance, and sismofaceis. The erosional surface mapped from shallow seismic sections is possibly a indicative of an ancient surface of valley incision, where it would probably be associated with the limit Pleistocene/Holocene. Different sismofaceis were identified and reflect the rise in sea level with standards sometimes agradacional, sometimes progradational. The thickness of sediments on this surface was estimated at a maximum of 22m thick in the central portion of the incised valley. Statistically, there are differences between the adjacent continental shelf and channels, and between these channels, for the content of calcium carbonate, organic matter, sand and mud perceptual, except for the gravel grain size. The analysis of living and dead foraminifera showed the presence of fifty species distributed in regards to morphology, depth and type of sediment. Four type of seismic echocharacteres were identified and mapped, as well as their bedforms, indicating different sedimentary processes along the incised valley. The integration of results suggests an activation of the Apodi-Mossoró incised valley in the Late Pleistocene.

O sistema aquífero Barreiras na região de Parnamirim, RN: uso das águas e potencialidades

The study area is within the Pirangi River Basin, eastern sector of Rio Grande do Norte state, where is located of the Parnamirim city. It has an area of approximately 370 km². Urbanization has developed much fast without an appropriate infrastructure, mainly by the lack of sewage systems, with risks of contamination of groundwater that may cause serious damage to the health of the population. The Barreiras Aquifer System groundwater in the area represents the main source of water supply for urban and rural populations. The use of groundwater occurs without adequate planning and therefore, important recharge areas are being occupied. This study was conducted to quantify the use and evaluation of the potential of groundwater, in order to increase good water quality supply and lower risks of being affected by polluting activities. With these objectives, the following activities were carried out: 268 points of water have been registered; characterization of the lithological, thickness and hydrogeological structure of the Barreiras aquifer, based on the correlation of well logs; and evaluation of hydrodynamic parameters of the aquifer, from the interpretation of results well pumping tests. It was found that the saturated thickness increases from west to east towards the sea, with values ranging from 15,47-56,5 m with an average of 32,45 m. The hydrodynamic parameters using Cooper-Jacob method were: average transmissivity of 5,9x10-3 m²/s and average hydraulic conductivity 2,82x10-4 m/s. The effective porosity is of 15%, obtained by applying Biecinski equation. The potentiometric map shows the main direction of groundwater flow, from west to east, and identifies the recharge areas corresponding to the region of the tablelands of the "Barreiras". The river valleys refer to the discharge areas of the aquifer system. The Recharge was estimated at 253 mm/year, which corresponds to the 16.4% rate of infiltration.

O sistema aquífero Barreiras na região de Parnamirim, RN: uso das águas e potencialidades

The study area is within the Pirangi River Basin, eastern sector of Rio Grande do Norte state, where is located of the Parnamirim city. It has an area of approximately 370 km². Urbanization has developed much fast without an appropriate infrastructure, mainly by the lack of sewage systems, with risks of contamination of groundwater that may cause serious damage to the health of the population. The Barreiras Aquifer System groundwater in the area represents the main source of water supply for urban and rural populations. The use of groundwater occurs without adequate planning and therefore, important recharge areas are being occupied. This study was conducted to quantify the use and evaluation of the potential of groundwater, in order to increase good water quality supply and lower risks of being affected by polluting activities. With these objectives, the following activities were carried out: 268 points of water have been registered; characterization of the lithological, thickness and hydrogeological structure of the Barreiras aquifer, based on the correlation of well logs; and evaluation of hydrodynamic parameters of the aquifer, from the interpretation of results well pumping tests. It was found that the saturated thickness increases from west to east towards the sea, with values ranging from 15,47-56,5 m with an average of 32,45 m. The hydrodynamic parameters using Cooper-Jacob method were: average transmissivity of 5,9x10-3 m²/s and average hydraulic conductivity 2,82x10-4 m/s. The effective porosity is of 15%, obtained by applying Biecinski equation. The potentiometric map shows the main direction of groundwater flow, from west to east, and identifies the recharge areas corresponding to the region of the tablelands of the "Barreiras". The river valleys refer to the discharge areas of the aquifer system. The Recharge was estimated at 253 mm/year, which corresponds to the 16.4% rate of infiltration.

Efeito do tratamento por plasma na proliferação de fibroblastos e esterilização de membranas de quitosana

Chitosan is being studied for use as dressing due their biological properties. Aiming to expand the use in biomedical applications, chitosan membranes were modified by plasma using the following gases: nitrogen (N2), methane (CH4), argon (Ar), oxygen (O2) and hydrogen (H2). The samples were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), contact angle, surface energy and water absorption test. Biological Tests were also performed, such as: test sterilization and proliferation of fibroblasts (3T3 line). Through SEM we observed morphological changes occurring during the plasma treatment, the formation of micro and nano-sized valleys. MFA was used to analyze different roughness parameters (Ra, Rp, Rz) and surface topography. It was found that the treated samples had an increase in surface roughness and sharp peaks. Methane plasma treatment decreased the hydrophilicity of the membranes and also the rate of water absorption, while the other treatments turned the membranes hydrophilic. The sterilization was effective in all treatment times with the following gases: Ar, N2 and H2. With respect to proliferation, all treatments showed an improvement in cell proliferation increased in a range 150% to 250% compared to untreated membrane. The highlights were the treatments with Ar 60 min, O2 60 min, CH4 15 min. Observing the results of the analyzes performed in this study, it appears that there is no single parameter that influences cell proliferation, but rather a set of ideal conditions that favor cell proliferation