HIV/AIDS e estilo de vida: par��metros imunol��gicos, virol��gicos, s��ndrome lipodistr��fica e sua rela����o com a pr��tica de exerc��cios

This descriptive study aimed to investigate the relationship between expression of immunological (TCD4 +) and virological (viral load) parameters, lipodystrophy syndrome and lifestyle variables of people living with HIV who underwent a program of physicalexercise. Initially, the sample was composed by 17 persons, recorded at the Department of Giselda Trigueiro Hospital Care (GTH), Natal-RN . With the passing of the stages of intervention (physical training program- PTP) the number of participants has changed (17, 9, 8 and 7) as the training phases (I, II, III and IV). Data collected were on total cholesterol, High Density Lipoprotein (HDL) and triglycerides. The % fat (% F), body mass index (BMI), waist-hip ratio (WHR), lean mass (LM), central fat (BF), total (TF) and peripheral (PF), weight and height were used to measure the morphological parameters. For control of variables (TCD4 +, viral load, Triglycerides, Cholesterol, HDL), the information contained in the blood tests every four months were investigated. After Phase I and II, it was used a structured interview. Then sampling was carried out considering the pre-and post-tests 1, 2, 3 and 4 (after 16, 32, 48 and 64 weeks of training, respectively). Daily, the intensity of the work was checked by the scale of perceived exertion for exercises adapted to resistance34. Procedures used were descriptive statistics (dispersion, absolute and relative frequencies, means, standard deviations and minimum and maximum values) as well as Spearman linear correlation adopting a significance level of p &#8804; 0.05. Positive changes were observed for TCD4 + and viral load in all phases of the PTP. For the morphological components, the loss of central subcutaneous fat (CSF) and total subcutaneous fat (TSF) for both sexes and the decrease in % BF among women were the most dramatic results after the training phase I. For men, results were more significant to % F and LM in Phases I and II and peripheral subcutaneous fat (PSF) declined in all phases of the exercise. As for women, results were more expressive for % F and TM in the phases I and II and the PSF decreased in all phases of the exercise. Whereas for women, the waist/hip ratio (WCQ) and 0% F decreased and showed a positive association with triglycerides (WHR r * 0.82, p 0.042, r 0.88 TSF *, p 0.019 and r 1.00 ** CSF, p <0.001) and among men with limb subcutaneous fat (LSF)* r 0.65, p 0.029). The PTP provided improvement in the health, self-esteem and quality of life, proving to be a possible strategy to positively influence the expression of immunological parameters (TCD4 +) and virological (viral load) and morphological components of people living with HIV causing no deleterious effects in these parameters

Exerc��cios anaer��bicos, padr��o morfofuncional e os marcadores hematol��gicos em pessoas vivendo com hiv e aids

Estudos que relacionam a atividade f��sica e HIV/AIDS enfatizam a perspectiva biol��gica, demonstrando a sua relev��ncia, apontam a preocupa����o em estabelecer um tipo de atividade e intensidade que n��o comprometa o sistema imunol��gico, enfatizando as melhorias do exerc��cio sobre os par��metros antropom��tricos, na aptid��o f��sica bem como um grande aumento da capacidade funcional para o grupo testado. Objetivo: O presente estudo se caracteriza como quase-experimental e objetivou analisar o padr��o morfofuncional, marcadores hematol��gicos em pessoas vivendo com HIV/AIDS, atrav��s da oferta interven����o com exerc��cios ana��robicos (exerc��cios resistidos em sala de muscula����o) para pacientes portadores de HIV/AIDS. M��todos: A amostra foi composta por 11 indiv��duos do g��nero masculino, com m��dia de idade de 43,2 anos, participantes do Programa Pro-Sa��de e Atividade F��sica da Universidade Federal do Rio grande do Norte e atendimento cl��nico no Hospital Giselda Trigueiro da Secretaria de Estado de Sa��de P��blica/RN. A coleta de dados foi composta de exames laboratoriais para medir TCD4 e Carga Viral, medidas antropom��tricas: ��ndice de Massa Corporal - IMC, Rela����o Cintura Quadril- RCQ, e uma annamenese sociodemogr��fica. Para an��lise dos dados utilizou-se no presente estudo a estatistica descritiva com os valores de tendecia central e seus derivados. Para as vari��veis de cunho discreto utilizou-se a an��lise de frequencia atav��s de valores percentuais de aparecimento. Resultados: Os resultados entre os Pr�� e o P��s teste das vari��veis estudadas foram assim apresentadas: para o IMC (pr�� m��dia de 24,0 Kg/m2 e p��s 24,2 Kg/m2), RCQ (pr�� m��dia de 0,97cm e p��s 0,95 cm), Somat��rio de Dobras cut��neas (Pr�� 117,1 e P��s 102,0), Somat��rio da Perimetria(Pr�� 310,5 e P��s 313,3), For��a Escapular(Pr�� 30,9 Kgf e P��s 30,3 Kgf), For��a Press��o Manual Direita(Pre 41,2 e P��s 42,1), For��a Press��o Manual esquerda(Pr�� 39,4 e Pos 39,9), TCD4( Pr�� 579 e P��s 509 c��ls/mm3) e Carga Viral(pr�� e p��s <50 c��pias). Esses resultados apontam para uma melhora importante das vari��veis antropom��tricas estudadas, principalmente em rela����o a RCQ, na contagem dos linf��citos TCD4 e manuten����o da Carga Viral em n��veis abaixo do limite m��nimo(<50 c��pias/ml) Conclus��o:O Programa de Exerc��cios resistidos com caracter��sticas anaer��bicas promove melhorias no padr��o morfofuncional, manuten����o das c��lulas TCD4 em n��veis considerados seguros para esta popula����o e estabiliza����o da carga viral, n��o apresentando riscos �� sa��de dos participantes

Caracteriza����o do perfil imunol��gico em mulheres portadoras do papilomav��rus humano

O c��ncer de colo uterino consiste em um problema de grande relev��ncia social, visto que se trata da segunda malignidade ginecol��gica mais freq��ente no mundo. O principal precursor do c��ncer da c��rvice uterina consiste na infec����o pelo Papiloma v��rus humano (HPV). O HPV tem despertado grande interesse na comunidade cient��fica, devido �� rela����o estabelecida entre a sua epidemiologia e o c��ncer de c��rvice uterina. Apesar do grande avan��o na biologia do HPV, pouco se sabe acerca da resposta imunol��gica a este v��rus. O Papilomav��rus humano (HPV) �� um DNA v��rus epiteliotr��fico, que est�� vinculado �� carcinog��nese do colo uterino por meio de evid��ncias epidemiol��gicas e laboratoriais. As infec����es pelo HPV ocorrem em mulheres em todo o mundo. Existe consenso na literatura quanto �� associa����o do DNA HPV com as neoplasias intra epiteliais cervicais e o c��ncer cervical. Por este motivo �� de grande import��ncia a detec����o precoce deste tipo de infec����o para que seja efetuado um tratamento espec��fico. Esse trabalho est�� caracterizando o perfil imunol��gico de pacientes portadoras de HPV atrav��s da quantifica����o linfocit��ria (TCD4, TCD8, c��lulas NKT e c��lulas NK), e correlacionou esse material �� t��cnica de captura h��brida, para poder comprovar a infec����o pelo HPV, esperando assim contribuir precocemente para o diagn��stico do c��ncer de colo uterino.

Receptores de citocinas proinflamat��rias na pr��-ecl��mpsia

Preeclampsia is a disease specific of human pregnancy that affects 3-8% of pregnant women, and it is one of the three leading causes of maternal mortality and morbidity. The disease is characterized by hypertension and proteinuria after the 20th week of gestation. The risk factors for this disease are not completely understood but appear to include dysregulation of the immune response arising from defects in placentation, environmental and genetic factors. This study aimed to determine whether the variation in the amount of proinflammatory cytokine receptors IL-1R2, IL-6R and TNF-��R1 would be involved in preeclampsia. They were recruited women with preeclampsia (n=24) and women who evolved during pregnancy without changes in blood pressure (n=12) were recruited. Clinical and laboratory data were collected. The cytokine receptors (IL-1R2, TNF-��R1 and IL-6R) were assessed in mononuclear cells isolated from peripheral blood using flow cytometry (Control = 8; PE = 24). C-reactive protein (CRP) was determined by CRP ultrasensitive method (Control = 7; PE = 18) was performed using sera pregnant women. Women with preeclampsia had higher weight at the beginning of the pregnancy (p=0.0171) and lower gestational age at delivery (0.0008). Classical monocytes were decreased in preeclampsia but not intermediate or non-classical monocytes. The frequency of IL-1R2 pro inflammatory cytokine receptors is decreased in women with PE only in the subpopulation of non-classical monocytes (p = 0.0011). TNF-��R1 receptor and IL-6R, had a decreased frequency in the three subpopulations of monocyte (classic, intermediate and non-classical) when compared to women with normal pregnancy. An increase in IL-1R2 receptor in TCD4+ lymphocytes, but a decrease in TNF-receptor and IL-6R in women with preeclampsia were found. No differences in the frequency of those receptors in CD3+/CD8+ in preeclampsia. There was no difference in C-reactive protein in preeclampsia. The reduction in the amount of IL-1R2, TNF- ��R1 and IL-6R monocytes and lymphocytes can be involved in the regulation of inflammation observed in preeclampsia, contributing to disease.