Estudo in silico da interação da albumina de soro humano com o ibuprofeno

Currently, computational methods have been increasingly used to aid in the characterization of molecular biological systems, especially when they relevant to human health. Ibuprofen is a nonsteroidal antiinflammatory or broadband use in the clinic. Once in the bloodstream, most of ibuprofen is linked to human serum albumin, the major protein of blood plasma, decreasing its bioavailability and requiring larger doses to produce its antiinflamatory action. This study aimes to characterize, through the interaction energy, how is the binding of ibuprofen to albumin and to establish what are the main amino acids and molecular interactions involved in the process. For this purpouse, it was conducted an in silico study, by using quantum mechanical calculations based on Density Functional Theory (DFT), with Generalized Gradient approximation (GGA) to describe the effects of exchange and correlation. The interaction energy of each amino acid belonging to the binding site to the ligand was calculated the using the method of molecular fragmentation with conjugated caps (MFCC). Besides energy, we calculated the distances, types of molecular interactions and atomic groups involved. The theoretical models used were satisfactory and show a more accurate description when the dielectric constant ε = 40 was used. The findings corroborate the literature in which the Sudlow site I (I-FA3) is the primary binding site and the site I-FA6 as secondary site. However, it differs in identifying the most important amino acids, which by interaction energy, in order of decreasing energy, are: Arg410, Lys414, Ser 489, Leu453 and Tyr411 to the I-Site FA3 and Leu481, Ser480, Lys351, Val482 and Arg209 to the site I-FA6. The quantification of interaction energy and description of the most important amino acids opens new avenues for studies aiming at manipulating the structure of ibuprofen, in order to decrease its interaction with albumin, and consequently increase its distribution

Crises do cotidiano em Machado de Assis e Luigi Pirandello: um estudo comparado

This thesis proposes to identify possible similarities and differences between the novels Memórias Póstumas de Brás Cubas (1981), by the Brazilian writer Machado de Assis, and Uno, Nessuno and Centomila (1926), by the Italian writer Luigi Pirandello. The two authors have prominent respective places in Brazilian and Italian literature, and both observed and experienced remarkable changes in their societies Brazil's imperial period, and Italy’s post-risorgimentale period. We will verify how each author composes a piece o literary art in which it is possible to achieve the authors’social and moral conscience. We will also attempt to achieve thefeelings of restlessness, anxiety, fear, doubt, interest, vanity, ambition: in summary, the desire of the characters to exist, which represents man at the end of the nineteenth century and commencement of thetwentieth century. The characters’ features point to fragmented identity, in search of a place in the world, even if he needs to renounce his essence and to adopt a corresponding appearance to all images that society assigns to him in order to achieve such a place. The protagonists, Brás Cubas and Vitangelo Moscarda, will lead us along the paths of consciousness of each, which demarcate the border between essence and appearance. Critics such as Roberto Schwarz, Alfredo Bosi, Leone Castris, among others, will give us the theoretical support necessary for a comparative study between the two authors, whom, like few others, knew how to express, through their characters, the difficult relationship of man with himself and with the universe at large.

Intercompreensão de línguas românicas: uma proposta para a leitura literária plurilíngue no ensino fundamental

The developed research aimed to investigate how students behave on elementary school in a plurilingual context, based on Intercomprehension of Romanic Languages with literary texts in the classroom. The theoretical framework prioritizes authors who consider reading literary text as an essential tool to the formation of reading and writing skills for students, such as: Amarilha (1997; 2003; 2007), Resende (1993), Kleiman, (1999), Villardi (1997), Aguiar (1991), Perrone-Moisés (2000), Lajolo (1993), Zilberman (1991), Cosson (2006), Andrade, Melo-Pfeifer, Santos, (2009), Sá; De Carlo; Antoine (2011), Alas Martins (2014), Doyé (2005), Souza, (2013), and others, according to the intercomprehension approach and plurilingualism. We use questionnaires as a methodological resources for this research, and we applied some activities that was developed based on intercomprehension from literary texts which represent three types of Romanic languages (Spanish, French and Italian), in addition to the participant observation in classes with students of the 8th grade from an elementary public school in the city of Natal (RN). The students could read and (inter) understand some texts of classic literature in those Latin languages and also in Portuguese, whose titles include “D. Quixote de la mancha” by Miguel de Cervantes; “O pequeno príncipe” by Antoine de Saint-Exupéry; and “Pinóquio” by Carlo Collodi. The data analysis shows that students understood the lessons with plurilingual texts as something which goes beyond the structure of language teaching, awakening themselves to the knowledge of new languages and cultures, and linguistic diversity as motivation at the time of understanding and literature as transforming element to Citizen formation of students.