Transcriptoma diferencial entre células-tronco mesenquimais humanas jovens e senescentes

Human mesenchymal stem cells (MSC) are powerful sources for cell therapy in regenerative medicine. The long time cultivation can result in replicative senescence or can be related to the emergence of chromosomal alterations responsible for the acquisition of tumorigenesis features in vitro. In this study, for the first time, the expression profile of MSC with a paracentric chromosomal inversion (MSC/inv) was compared to normal karyotype (MSC/n) in early and late passages. Furthermore, we compared the transcriptome of each MSC in early passages with late passages. MSC used in this study were obtained from the umbilical vein of three donors, two MSC/n and one MSC/inv. After their cryopreservation, they have been expanded in vitro until reached senescence. Total RNA was extracted using the RNeasy mini kit (Qiagen) and marked with the GeneChip ® 3 IVT Express Kit (Affymetrix Inc.). Subsequently, the fragmented aRNA was hybridized on the microarranjo Affymetrix Human Genome U133 Plus 2.0 arrays (Affymetrix Inc.). The statistical analysis of differential gene expression was performed between groups MSC by the Partek Genomic Suite software, version 6.4 (Partek Inc.). Was considered statistically significant differences in expression to p-value Bonferroni correction ˂.01. Only signals with fold change ˃ 3.0 were included in the list of differentially expressed. Differences in gene expression data obtained from microarrays were confirmed by Real Time RT-PCR. For the interpretation of biological expression data were used: IPA (Ingenuity Systems) for analysis enrichment functions, the STRING 9.0 for construction of network interactions; Cytoscape 2.8 to the network visualization and analysis bottlenecks with the aid of the GraphPad Prism 5.0 software. BiNGO Cytoscape pluggin was used to access overrepresentation of Gene Ontology categories in Biological Networks. The comparison between senescent and young at each group of MSC has shown that there is a difference in the expression parttern, being higher in the senescent MSC/inv group. The results also showed difference in expression profiles between the MSC/inv versus MSC/n, being greater when they are senescent. New networks were identified for genes related to the response of two of MSC over cultivation time. Were also identified genes that can coordinate functional categories over represented at networks, such as CXCL12, SFRP1, xvi EGF, SPP1, MMP1 e THBS1. The biological interpretation of these data suggests that the population of MSC/inv has different constitutional characteristics, related to their potential for differentiation, proliferation and response to stimuli, responsible for a distinct process of replicative senescence in MSC/inv compared to MSC/n. The genes identified in this study are candidates for biomarkers of cellular senescence in MSC, but their functional relevance in this process should be evaluated in additional in vitro and/or in vivo assays

Transplantes: re-inserção social e qualidade de vida dos transplantados cardíacos do Estado do RN

The experience of transplantation is a very serious situation from the clinical standpoint. Therefore, there must be some subjective and social breakdown in people who have been undergone such a procedure. Recent product of modernity, the transplanted is someone who owns his recover to the scientific advances of contemporary society and a deceased donor. This paper aims at examine the implications, from the changes in patterns of behavior and thought that occur after the experience of an extreme and critical situation, as the process of illness and its consequent transplant surgery. The symbolism of the heart suggests that some social impressions about the organ itself are also reflected in how the transplanted interprets the experience of this type of procedure. So investigating how the changes occurred throughout the process interfere in the re-insertion of these people to social life, after his recovery, is the purpose of this work. The concept of habitus coined by Pierre Bourdieu will be used to measure conceptually how this experience (clinical, modern and symbolic) fits in the contemporary discussion of sociology

O poema e a canção em as coisas, de Arnaldo Antunes: imagens da primeiridade

Ce travail a pour but d analyser un corpus de six textes hybrides, que nous classons comme des poèmes-chansons/ poèmes-chantés à cause de leur double présence dans deux systèmes sémiotiques différents. Le premier, la littérature, ou plus spécifiquement la poésie, a comme support le livre As Coisas [Les Choses], d Arnaldo Antunes et l autre, la chanson, est enregistré dans les disques du même auteur. Notre travail lance un regard sur ce corpus, en essayant de vérifier un aspect recourrent dans l oeuvre d Arnaldo Antunes qui est la présence da priméité, catégorie theórique développée par Charles Sanders Peirce. Au-delà de l observation de cet aspect sémiothique, nous ferons une discussion sur la chanson populaire, et ses rapports avec la poésie et par conséquent avec la Litterature. La théorie sémiothique s appuyera sur deux piliers : En ce qui concerne l étude de la priméité, nous travaillerons avec les théories de Peirce, mais en nous servant aussi des ouvrages de Lúcia Santaella, Winfried Nöth, Júlio Plaza et Décio Pignatari ; Dans l autre voie, pour ce qui concerne l analyse des chansons, nous utiliserons la théorie de Luiz Tatit, fondée sur la sémiothique de Algirdas Julien Greimas. Tatit trace une méthode d analyse, où il est possible d analyser une chanson en exploitant et le texte et la mélodie, ce qui permet une meilleure compréhension de l étude des poèmes-chansons et ses variations. Comme support pour la discussion sur la musique, nous nous servirons des théories de José Miguel Wisnik, Claude Lévi-Strauss, Roland Barthes et Jean Fisette