Lesões orais com potencial de malignização: análise clínica e morfológica de 205 casos

Silveira , E. J. D. et al. Lesões orais com potencial de malignização: análise clínica e morfológica de 205 casos. J. Bras. Patol. Med. Lab., v. 45, n. 3, p. 233-238, jun 2009. ISBN 1676-2444.

Funding was obtained from a Capes Fellowship to NM, grants CNPq Universal 481351/ 2011-6, CNPq PQ 306604/2012-4, FAPERN/CNPq Pronem 003/2011, Capes SticAmSud, and FAPESP/CEPID/Neuromat to S.R. CNPq Universal 473554/2011-9 and 480053/ 2013-8, CNPq PQ 308558/2011-1, FACEPE/CNPq-PRONEX APQ- 0203-1.05/08, FACEPE/CNPq-PRONEM APQ-1415-1.05/10, and CNAIPS to M.C

Funding was obtained from a Capes Fellowship to NM, grants CNPq Universal 481351/ 2011-6, CNPq PQ 306604/2012-4, FAPERN/CNPq Pronem 003/2011, Capes SticAmSud, and FAPESP/CEPID/Neuromat to S.R. CNPq Universal 473554/2011-9 and 480053/ 2013-8, CNPq PQ 308558/2011-1, FACEPE/CNPq-PRONEX APQ- 0203-1.05/08, FACEPE/CNPq-PRONEM APQ-1415-1.05/10, and CNAIPS to M.C

Análise do efeito de polimorfismos não-sinônimos em genes de reparo de DNA da via BER na resposta inflamatória da meningite

In vitro and in animal models, APE1, OGG1, and PARP-1 have been proposed as being involved with inflammatory response. In this work, we have investigated if the SNPs APE1 Asn148Glu, OGG1 Ser326Cys, and PARP-1 Val762Ala are associated to meningitis and also developed a system to enable the functional analysis of polymorphic proteins. Patients with bacterial meningitis (BM), aseptic meningitis (AM) and controls (non-infected) genotypes were investigated by PIRA-PCR or PCR-RFLP. DNA damages were detected in genomic DNA by Fpg treatment. IgG and IgA were measured from plasma and the cytokines and chemokines were measured from cerebrospinal fluid samples using Bio-Plex assays. The levels of NF-κB and c-Jun were measured in CSF by dot blot assays. A significant (P<0.05) increase in the frequency of APE1 148Glu allele in BM and AM patients was observed. A significant increase in the genotypes Asn/Asn in control group and Asn/Glu in BM group was also found. For the SNP OGG1 Ser326Cys, the genotype Cys/Cys was more frequent (P<0.05) in BM group. The frequency of PARP-1 Val/Val genotype was higher in control group (P<0.05). The occurrence of combined SNPs increased significantly in BM patients, indicating that these SNPs may be associated to the disease. Increasing in sensitive sites to Fpg was observed in carriers of APE1 148Glu allele or OGG1 326Cys allele, suggesting that SNPs affect DNA repair activity. Alterations in IgG production were observed in the presence of SNPs APE1Asn148Glu, OGG1Ser326Cys or PARP-1Val762Ala. Reductions in the levels ofIL-6, IL-1Ra, MCP-1/CCL2and IL-8/CXCL8 were observed in the presence of APE1148Glu allele in BM patients, however no differences were observed in the levels of NF-κB and c-Jun considering genotypes and analyzed groups. Using APE1 as model, a system to enable the analysis of cellular effects and functional characterization of polymorphic proteins was developed using strategies of cloning APE1 cDNA in pIRES2-EGFP vector, cellular transfection of the construction obtained, siRNA for endogenous APE1 and cellular cultures genotyping. In conclusion, we obtained evidences of an effect of SNPs in DNA repair genes on the regulation of immune response. This is a pioneering work in the field that shows association of BER variant enzymes with an infectious disease in human patients, suggesting that the SNPs analyzed may affect immune response and damage by oxidative stress level during brain infection. Considering these data, new approaches of functional characterization must be developed to better analysis and interactions of polymorphic proteins in response to this context

A intervenção estatal no domínio econômico privado através da nova lei de recuperação de empresas e falências (lei 11.101/05)

Analysis of the elements of the Constitutional Order of the letter 1988 politics, with emphasis in the principles of this, a study on the intervention of the State in the private initiative by means of the Law of Recovery of Companies and Bankruptcies (law 11.101/05). New enterprise vision is admitted, over all in the interdependence between economic and social factors. Study on the globalization and the interdependence of economic and legal sciences in the construction of a legal optics in the search for the economic and social development, with the recognition of the interference of the Economy in the Right and its uneven importance. Still, we delineate the state intervention in the economic scope, of company and in the judicial recovery, as well as the consequences of such intervention in the involved credits in the judicial recovery and patrimony of the debtor in recovery. For such task, the elements of the Judicial Recovery, its principles and adequacy of these to the related ones in the chapter had been analyzed that turns on the national economic Order, describing the formal procedure for concession of the benefit of the Judicial Recovery and the principles in existing them. The forms of intervention of the State in the private economy were not disrespected, relating its direct and indirect performance as half of preservation of interests writings in the constitutional scope as public interest and preservation of the National economic Order. The regulating agencies as of direct state intervention were half not disrespected of the study for the relevance of the subject. It is revised national bibliography with incursions in French, Portuguese and North American comparative jurisprudence. One contributes in the aspect of the paper of the Judiciary Power in the protection of the companies in crisis and the social and economic impacts, over all in relation to the rights of the worked ones, credit and enterprise

Representações discursivas no discurso político. Não me fiz sigla e legenda por acaso : o discurso de renúncia do senador Antonio Carlos Magalhães (30/05/2001)

O objetivo deste trabalho é investigar como as representações discursivas do locutor e dos alocutários são construídas no discurso de renúncia ao mandato de senador, proferido por Antonio Carlos Magalhães (ACM), na 62ª sessão deliberativa ordinária, em 30/5/2001. A perspectiva teórica que adotamos situa-se no campo da Linguística do Texto, com foco na abordagem da Análise Textual dos Discursos ATD (Adam, 2011). A noção de representação discursiva apresentada pela ATD constitui um dos aspectos da dimensão semântica do texto e baseia-se parcialmente nos trabalhos de Grize sobre a esquematização (1990, 1996). Complementamos as categorias de análise com contribuições que nos permitem detalhar a expressão linguística, textual, e discursiva das representações discursivas (Neves, 2006; Castilho 2010; Rodrigues, Passeggi, Silva Neto, 2010; Rodrigues et al. 2012; Passeggi, 2012). O enfoque metodológico é qualitativo, descritivo e interpretativista (Minayo, 1994; Trivinõs; 1987; Gil, 2002). Os procedimentos de análise utilizam as categorias semânticas de referenciação, predicação, modificação (de referentes e predicações), localização espacial e temporal, conexão e comparação. A representação discursiva do locutor (ACM) é constituída pelo conjunto de representações mais específicas, expressas nas referenciações e nas suas modificações: vítima; político; sigla; baiano, nordestino; presidente do senado; senador confiante; condenado. ACM, como protagonista, assume sempre a sua voz no discurso, manifesta seus pontos de vista e posiciona-se como sujeito ativo, consciente da importância do seu papel político e social, que o torna alvo e vítima das ações dos adversários. Complementando essa referenciação, as predicações e suas modificações se expressam através de verbos de ação, em especial, verbos na 1ª pessoa do singular que marcam o tempo presente, real e conclusivo de ações que constroem um cenário positivo para si mesmo. A localização temporal e espacial, indica as ações realizadas pelos participantes nas diversas etapas temporais selecionadas pelo texto, articuladas a três espaços principais: o Senado Federal, o Brasil e, naturalmente, a Bahia. Por sua vez, conectores adversativos (sobretudo, mas ), explicativos e condicionais acompanham e estruturam o ritmo argumentativo do discurso de renúncia de ACM