Ecogenômica de archaea e monitoramento de comunidades de procariotos redutores de sulfato: aplicações na indústria de petróleo e gás

The human activities responsible for the ambient degradation in the modern world are diverse. The industrial activities are preponderant in the question of the impact consequences for brazilian ecosystems. Amongst the human activities, the petroliferous industry in operation in Potiguar Petroliferous Basin (PPB) displays the constant risk of ambient impacts in the integrant cities, not only for the human populations and the environment, but also it reaches the native microorganisms of Caatinga ground and in the mangrove sediment. Not hindering, the elaboration of strategies of bioremediation for impacted areas pass through the knowledge of microbiota and its relations with the environment. Moreover, in the microorganism groups associated to oil, are emphasized the sulfate-reducing prokaryotes (SRP) that, in its anaerobic metabolism, these organisms participate of the sulfate reduction, discharging H2S, causing ambient risks and causing the corrosion of surfaces, as pipelines and tanks, resulting in damages for the industry. Some ancestries of PRS integrate the Archaea domain, group of microorganisms whose sequenced genomes present predominance of extremophilic adaptations, including surrounding with oil presence. This work has two correlated objectives: i) the detection and monitoring of the gene dsrB, gift in sulfate-reducing prokaryotes, through DGGE analysis in samples of mDNA of a mangrove sediment and semiarid soil, both in the BPP; ii) to relate genomic characteristics to the ecological aspects of Archaea through in silico studies, standing out the importance to the oil and gas industry. The results of the first work suggest that the petrodegraders communities of SRP persist after the contamination with oil in mangrove sediment and in semiarid soil. Comparing the populations of both sites, it reveals that there are variations in the size and composition during one year of experiments. In the second work, functional and structural factors are the probable cause to the pressure in maintenance of the conservation of the sequences in the multiple copies of the 16S rDNA gene. Is verified also the discrepancy established between total content GC and content GC of the same gene. Such results relating ribosomal genes and the ambient factors are important for metagenomic evaluations using PCR-DGGE. The knowledge of microbiota associated to the oil can contribute for a better destination of resources by the petroliferous industry and the development of bioremediation strategies. Likewise, search to lead to the best agreement of the performance of native microbiota in biogeochemical cycles in Potiguar Petroliferous Basin ecosystem

Avaliação da cobertura e monitoramento do branqueamento de corais nos

Coral bleaching has been increasingly the focus of research around the world since the early 1980s, when it was verified to be increasing in frequency, intensity and amount of areas affected. The phenomenon has been recorded since 1993, associated with elevation of the sea surface temperature due to El Niños and water thermal anomalies, according to most reports around the world. On the coast of Rio Grande do Norte, Brazil, a mass coral bleaching event was recorded in the Environmental Protection Area of Coral Reefs (APARC) during March and April 2010, when the water temperature reached 34°C for several days. About 80% of the corals in Maracajaú reef-complex exhibited partial or total bleaching. The aims of this study were to verify representativeness of coral coverage and how the bleaching dynamic has developed among different species. Coral coverage was estimated according to Reef Check Brazil protocol associated with quadrant method, and bleaching was evaluated from biweekly visual surveys in 80 colonies of Favia gravida, Porites astreoides, Siderastrea stellata and Millepora alcicornis. At the same time temperature, pH, salinity and horizontal transparency, as well as mortality and disease occurrence were monitored. Analysis of variance and Multiple Regression from the perspective of time lag concept were used to evaluate the bleaching dynamics among species and the relationship between variation of means of bleaching and variations of abiotic parameters, respectively. Species showed significant differences among themselves as to variation of means of bleaching over time, but the dynamic of variation exhibited similar patterns

A utilização do controle estatístico do processo para o monitoramento do sangue: estudo de caso no Memonorte-RN

The present work is grounded basically on the use of the Basic Tools for the Statistic Process Control SPC, with the intent to detect non-conformities on a given productive process. It consists on a case study accomplished at a Hemocenter in Natal (Rio Grande do Norte). In this study it is shown that, the Statistic Process Control Technique, which was used as a tool, is useful to identify on-conformities on the volume of hemocomponents. The gathering of the used data was performed by means of document analysis, direct observations and database queries. The results achieved from the study show that the analyzed products, even though when they have presented, in some cases, points out of control, they satisfied the ANVISA standards. Finally, suggestions for further improvement of the final product and guidance for future employment of CEP, also extended to other lines of production, are presented

Utilização do controle estatístico do processo para monitoramento do peso médio de cápsulas de Tuberculostáticos: estudo de caso no NUPLAM- RN

This Master Thesis presents a case study on the use of Statistical Process Control (SPC) at the Núcleo de Pesquisas em Alimentos e Medicamentos (NUPLAM). The SPC basic tools have been applied in the process of the tuberculostáticos drugs encapsulation, primarily concerning the objective to choose, between two speeds, which one is the best one to perform the tuberculostatics encapsulation. Later on, with the company effectively operating, the SPC was applied intending to know the variability of the process and, through the tracking of the process itself, to arrive at an estimated limit for the control of future lots of tuberculostatics of equal dosage. As special causes were detected acting in the process, a cause-and-effect diagram was built in order to try to discover, in each factor that composes the productive process, the possible causes of variation of the capsules average weight. The hypotheses raised will be able to serve as a base for deepened the study to eliminate or reduce these interferences in the process. Also a study on the capacity of the process to attend the specifications was carried out, and this study has shown the process´s inaptitude to take care of them. However, on the side of NUPLAM exists a real yearning to implant the SPC and consequently to improve the existing quality already present on its medicines

Fatores influenciadores da participação pública na tomada de decisão ambiental :o caso do programa de combate ao caramujo gigante (Achatina fulica) em Parnamirim/RN

The aim of this work is to investigate the factors which influence people s participation in the enviromental decision, in Parnamirim, Rio Grande do Norte, from the vision of the residents of that area, approaching the Plan of Control/Eradication of the African s Huge Snail (Achatina Fulica),with the jointly work of IBAMA and Municipal Town Hall of Parnamirim .The applied methodology consists of a research (Survey kind) including 395 interviews by people who live in that county, with minimum age of 18, within an universe of 124.690 residents. The choice of the county was due to a detection (made by IBAMA technicians and reported in a Technical Report) -which showed that the dangerous Snail is already spreading in 14 of 17 districts of the county-, as well as the support given by Parnamirim s Town Hall, with the implementation of the Plan Control/Eradication of the African s Huge Snail, widely known as Day C . The research tools used in that research consists of questionnaires with all sorts of questions. The results show us that most of the residents were feeling threatened by the presence of the animal as well as having had a little participation in the fight against the animal. It also shows us that residents believe that organizations (Town Hall, IBAMA and Local Community) involved are able to solve the problem and believe that the amounts which organization are supposed to take 89,4 %, 87,6% and 80,8%, in that order. As we check the results, we notice in 95%, the variety of threaten and frequency reunion and participation level

Efeitos da radiação uv, temperatura e vapor aquecido nos compósitos poliméricos: monitoramento, instabilidade estrutural e fratura

The application of composite materials and in particular the fiber-reinforced plastics (FRP) has gradually conquered space from the so called conventional materials. However, challenges have arisen when their application occurs in equipment and mechanical structures which will be exposed to harsh environmental conditions, especially when there is the influence of environmental degradation due to temperature, UV radiation and moisture in the mechanical performance of these structures, causing irreversible structural damage such as loss of dimensional stability, interfacial degradation, loss of mass, loss of structural properties and changes in the damage mechanism. In this context, the objective of this thesis is the development of a process for monitoring and modeling structural degradation, and the study of the physical and mechanical properties in FRP when in the presence of adverse environmental conditions (ageing). The mechanism of ageing is characterized by controlled environmental conditions of heated steam and ultraviolet radiation. For the research, it was necessary to develop three polymer composites. The first was a lamina of polyester resin reinforced with a short glass-E fiber mat (representing the layer exposed to ageing), and the other two were laminates, both of seven layers of reinforcement, one being made up only of short fibers of glass-E, and the other a hybrid type reinforced with fibers of glass-E/ fibers of curaua. It should be noted that the two laminates have the lamina of short glass-E fibers as a layer of the ageing process incidence. The specimens were removed from the composites mentioned and submitted to environmental ageing accelerated by an ageing chamber. To study the monitoring and modeling of degradation, the ageing cycles to which the lamina was exposed were: alternating cycles of UV radiation and heated steam, a cycle only of UV radiation and a cycle only of heated steam, for a period defined by norm. The laminates have already undergone only the alternating cycle of UV and heated steam. At the end of the exposure period the specimens were subjected to a structural stability assessment by means of the developed measurement of thickness variation technique (MTVT) and the measurement of mass variation technique (MMVT). Then they were subjected to the mechanical tests of uniaxial tension for the lamina and all the laminates, besides the bending test on three points for the laminates. This study was followed by characterization of the fracture and the surface degradation. Finally, a model was developed for the composites called Ageing Zone Diagram (AZD) for monitoring and predicting the tensile strength after the ageing processes. From the results it was observed that the process of degradation occurs Abstract Raimundo Nonato Barbosa Felipe xiv differently for each composite studied, although all were affected in certain way and that the most aggressive ageing process was that of UV radiation, and that the hybrid laminated fibers of glass-E/curaua composite was most affected in its mechanical properties

Monitoramento por dgps e análise dos processos erosivos da linha de costa na praia de Pirangi do Norte - Parnamirim / RN

Through out the course of a steady increase in search and recovery of space in the coastal zone, there is also an expanding concern about the erosion processes of this area. The main problem in coastal areas is that urbanization occurs in a disorderly fashion and unsustainable, further aggravating the problems of coastal dynamics. The study area of this work is located on the southern coast of Pirangi do Norte beach to about 20km south of Natal, capital of Rio Grande do Norte in the Parnamirim City. This area has the length of approximately 1km, divided into three sections (Western, Central and Eastern) with a morphology consisting of a tableland at the top, sea cliffs in the West and Central parts and sand dunes in the Eastern section, both vegetated, and a coastal plain on the inferior part associated with the presence of beach rocks. This study aimed to analyze the erosion processes operating in the excerpt of Pirangi do Norte beach and assess the feasibility of their monitoring making use of DGPS (Global Positioning System Differential mode). During the work it was carried out a physical description of the area through photo-interpretation and site survey after measurement of the shoreline in the period between November 2004 and November 2009 and beach profiles between August 2005 and July 2006. The analysis of the results of the annual surveys showed the occurrence of variations of the shoreline along the stretch traveled. Sites are identified in advancement coast from the sea and it was verified in loco the presence of erosion with deposition of materials on the lower part of the coastal bluff, the former position of the shoreline, showing a false notion of advancing it. This leads to the conclusion that the data collected in a survey of the shoreline should always be accompanied by photographic records of the local area and with the highest rate of erosion, thus avoiding the mistake of treating the deposit materials as evidence of progress coast. At the end of the study, after a review of various works to mitigate the erosion in the coastal zone, it is recommended to the area of study the adoption of an artificial feeding of the beach, aiming the minimization of the erosive effects of the tides. Moreover, it is suggested that even the continuity of monitoring, maintenance of existing vegetation and control of the occupation on the edge of sea cliffs

Controle sanitário e ambiental de um projeto de reúso em um complexo de prédios corporativos

The study that resulted in this dissertation was developed at OU RNCE PETROBRAS, in Natal, which implemented a project of rational use and reuse of water, including use of wastewater from a Sewage Treatment Plant (STP) already in place, diluted with water from own wells for irrigation of green area of the building complex corporate enterprise. Establish a methodology that can serve as guidelines for future projects controlled reuse of water like this was the objective of this research. Been proposed, implemented and evaluated three instruments of sanitary and environmental control: 1) adaptation of sewage treatment plant and quality control of the treated effluent 2) analysis of soil-nutrient interaction in the irrigated area, 3) knowledge of the local hydrogeology, especially with regard to the direction of flow of the aquifer and location of collection wells of Companhia de Águas e Esgotos do Rio Grande do Norte (CAERN) situated in the surroundings. These instruments have proven sufficient and appropriate to ensure the levels of sanitary and environmental control proposed and studied, which were: a) control of water quality off the STP and the output of the irrigation reservoir, b) control of water quality sub surface soil and assessment of progress on soil composition, c) assessment of water quality in the aquifer. For this, we must: 1) establishing the monitoring plan of the STP and its effluent quality sampling points and defining the parameters of analysis, improve the functioning of that identifying the adequacy of flow and screening as the main factors of operational control, and increase the efficiency of the station to a relatively low cost, using additional filters, 2) propose, implement and adapt simple collectors to assess the quality of water percolating into the soil of the irrigated area, 3) determine the direction of groundwater flow in the area study and select the wells for monitoring of the aquifer.

Usos e qualidade da água em região semi-árida do nordeste brasileiro: percepção ambiental de professores e alunos

Density and biomass of bacterioplankton in parts of the Marechal Dutra reservoir were studied in periods of dry and rainy season. The averages of the total bacterial densities oscillated in the order of 107 organisms/ mL of sample. The cocci cells were numerically predominant, influencing considerably the total abundance of the bacterioplankton. The total bacterial biomass, was considered raised and varied from 2.431,82 to 2.739,01 μgC.L-1, in function of the high values of biovolume of the filaments forms, and showed no marked spacial fluctuation. These high values of density and biomass alert for the necessity of a frequent monitoring of aquatic ecosystems of semi-arid region, where the quality of the water is affected by the occurrence of extensive dry periods, influencing considerably the quality of life of its population

Comunidade fitoplanctônica como discriminador ambiental em açude eutrófico tropical semiárido (Barragem Armando Ribeiro Gonçalves, RN) e divulgação científica para reflexão social

The knowledge of the phytoplankton community, as an integral and dynamic processes of eutrophication, provides information essential for proper management and handling. A growing problem of cyanobacteria in reservoirs around the world as a result of artificial eutrophication processes, generating a particular concern, because some species produce cyanotoxins, which can cause adverse effects on human health. The present work aims to characterize the spatial and temporal dynamics of phytoplankton, assessing their potential as ecological indicator of water quality in reservoirs semiarid region. The samples of water were collected monthly between 2009 and 2011, at three points along the dam Armando Ribeiro Gonçalves / RN. In each sample were measured physico - chemical analysis of water and biological components. We conducted a scientific dissemination activity, with distribution and reading primer on eutrophication, informative talk about water quality, questionnaires and performing a play in a public school in the city of Itajá / RN. The reservoir was considered eutrophic in three points, taking into account the values of chlorophyll -a and phosphorus, adopted to characterize eutrophic environments of semi-arid areas. High density of cyanobacteria, with a maximum value of 2.227.862 cél.ml- 1 and minimum of 43.456 cél.ml- 1 was recorded in lentic and semilêntico points throughout the study, exceeding the levels of drinking water (20.000 cél.ml- 1) established in 2.914/2011 Ordinance of the Ministry of Health of Brazil. All samples contained microcystin, and 44 % had values superiores1μg L- 1. The thermal pattern of the water column showed micro stratifications with differences of less than 1 ° C from five feet deep. The distribution pattern was the type profile clinogrado with oxygen deficit in the bottom of the reservoir. Oxiclina from 10 meters depth was observed during the rainy season (May-June) in the two years of study. The phytoplankton community was represented by 10 functional groups: S1, M, H1, Lo, P, F, Sn, P, W2 and R. The assessment of the ecological status of the system by the index Q showed poor water quality. The results of the study show that the vertical variations were less pronounced than the seasonal variations of cyanobacteria and phytoplankton community in general in the reservoir. The presence of cyanotoxins confirms the need for the monitoring of water quality and measures to reduce eutrophication in water supply reservoirs semiarid RN and demonstrates the challenge for water managers and health authorities to ensure water quality and consequently minimize risks to human health. Compared to the lecture, the primer was considered more efficient in sensitizing the participants, featuring a dynamic practice, differentiated learning, create opportunities for students to rethink attitudes of respect and care for the environment, and shall have the opportunity to learn the subject content from your reality and living environment. The knowledge generated from the activity of scientific were seen as essential for raising awareness of some of the region`s environmental problems , such as eutrophication

Avaliação do potencial de toxicidade de florações de cianobactérias como subsídio para ações de gestão ambiental em açudes do semiárido potiguar

Blooms negatively compromise the aquatic environment, plants, animals and human health.Some species are toxin-producing, such as the neurotoxin saxitoxin and the hepatotoxin microcystin, which may contaminate water reservoirs, as those existing in the semiarid region of Rio Grande do Norte (Brazil) which are used to supply the population, fishing, aquiculture and recreational activities, thereby providing the risk of human exposure through water intake, dermal contact and respiratory tract. Thus, it is recommended a constant monitoring of the density of cyanobacteria with the quantification of cyanotoxins. One goal with this work was the monitoring of water in four reservoirs in semiarid of RN through the identification and enumeration of cyanobacteria and through the identification and quantification of cyanotoxins by ELISA. Furthermore, we intended to assess the environmental perception of farmers and artisanal fishers in reservoirs of semiarid of RN through semi-structured interviews with questions mostly related to water and eutrophication. Through these objectives the aim was the development of management strategies for aquaculture and prevention of risks to public health. The results showed that the highest values of microcystins were found in the rainy season. Standards for drinking water, according to the guidelines of Ministry of Health 2914/2011 and CONAMA 357/05, setting the maximum values for raw water density of cyanobacteria: 50,000 cel.mL-1; microcystin: 1 μg. L-1 and saxitoxin: 3 μg. L-1. The values found for microcystin ranged between 0.00227 μg. L-1 and 24.1954 μg. L-1. From 128 samples analyzed, 27% were above the limit. There was no clear seasonal pattern for saxitoxins and their values ranged between 0.003 μg. L-1 and 0.766 μg. L-1 with none of the values above the limit. Furthermore, 76% of the densities of cyanobacteria values were above the limit. About environmental perception, 52 interviews were conducted and the results show that the respondents recognize the main uses of water of the rervoirs, recognize the importance and have a positive view about the reservoirs. They also realize that the water has a poor quality and can cause health problems. The results provide data showing the persistence of cyanobacteria and cyanotoxins, many times over the limit, reinforcing the importance of constant monitoring. The assessment of environmental perception gives foundation for later proposed environmental education linked to public health management into the context of this particular population, making it more effective

Caracterização geológica tridimensional e monitoramento de dunas no litoral oriental do Rio Grande do Norte

This work presents geophysical and geological results obtained in a dunefield located in the east coast of Rio Grande do Norte State, with the aim to recognize the aeolian body depositional geometries to a future geologic modeling of the aeolian petroliferous reservoirs. The research, which was done in blowouts region situated at Nisia Floresta Municipally, included the characterization of external geometries with GPS and internal geometry analysis by GPR. Data was integrated in GoCAD software, where it was possible the three-dimensional characterization and interpretation of the studied deposits. The interpretation of GPR profiling allowed identifying: First-order bounding surfaces that separated the aeolian deposits of the Barreiras Formation rocks; Second-order bounding surfaces, which limit dune generations and Third-order bounding surfaces, a reactivation surface. This classification was based and adapted by the Brookfield (1977) and Kocurek (1996) propose. Four radarfacies was recognized: Radarfacies 1, progradational reflectors correlated to foresets of the dunes, Radarfacies 2, plain parallels reflectors related to sand sheets, Radarfacies 3, plain parallels reflectors associated to reworking of the blowout dune crest and Radarfacies 4, mounded reflectors associated to vegetated mound of sand or objects buried in subsurface. The GPR and GPS methods was also employed to the monitoring of dunefields susceptible to human activities in Buzios Beach, where the constructions along the blowout region and the tourism are changing the natural evolution of the deposits. This fact possibly to cause negative impacts to the coastal zone. Data obtained in Dunas Park, a unit environmental conservation, was compared with information of the Buzios Beach. There is a major tendency of erosion in Buzios, specifically in blowout corridor and blowout dune

Análise e monitoramento geoambiental da praia de Genipabu/RN

Issues concerning coastal regions, especially the beaches have sparked quite complex because studies are there that most people in the world has secured housing, mainly from the half of the last century, without concern for the natural dynamics of these environments, which have complex interactions of continental and oceanic, coastal responsible for changes in locations that can be perceived in a few years and sometimes even a few days or hours. The search took as main goal, analyze the Genipabu Beach, in the municipality of Extremoz/RN, fragile environment and rapid momentum, which has been occupied in a disorderly and unplanned. Carried out a beach monitoring through profiles beach environments: defined stages morphodynamics; realization of characterize hydrodynamic processes; identification of changes in the landscape. To this end, made necessary a survey from the bibliographic collection for theoretical and conceptual rationale. An empirical step for conducting the environmental characterization of hydrodynamics, leveling and topographic analysis of sediments (in laboratory), for observation of changes in features, influenced, and natural dynamics, anthropic action that increasingly comes taking the characteristics from the natural landscape. Underlines therefore the importance of academic studies in several areas in these environments, for setting up a coastal zoning giving public subsidies for managers for managing and planning the use and occupation of the coast in their areas

Monitoramento da dinâmica costeira da praia de Ponta Negra

This dissertation includes the monitoring of coastal environmental dynamics at three points distinct from Ponta Negra beach, located on the South Coast of Natal, capital of Rio Grande do Norte, in the period June 2012 to May, 2013. For this, the following hypotheses were developed: Which actors morphodynamic and/or anthropogenic responsible for the changes in the study area? And yet, the configuration of the morphodynamic state of the beach, dissipative, reflective or intermediate? Faced with these questions , studies on the beach environment has its relevance as they may clarify the risks and responsibilities of anthropogenic intervention and also assist managers in more targeted action regarding the protection of praiais systems, since once committed, it is very difficult recover the environmental framework of the area, being greatly more feasible the development of multidisciplinary work plans that can guide human actions possible in search of an understanding to the harmonious interaction between society and the beach system. Its main goal is the understanding of the processes of coastal dynamics, methodological procedures that supported the implementation of this research were based on the object of study related literature associated with the collection of data resulting from beach profiles made monthly in spring tides (full moon), the hydrodynamic data and statistical quantification data (%) and size classification of sediment sediment after laboratory analysis. The results obtained from annual comparative tables of beach profiles, associated sedimentological analysis, indicated a positive sediment budget, tending to equilibrium for Point 01 and Point 02 negative. Have to Step 03 were added to the hydrodynamic data, which allowed also on a comparative framework, the perception of a depositional dynamics, with a tendency to decrease the accumulation of material at the end of the annual cycle. These data also allowed for the point 03, the calculation of the volume of material transported by the longshore current was around 104.280 m³/m, plus the Dean parameter which established a morphological state of the dissipative beach with specific prevalences for point 03. Thus, given the results presented in this work in a timely monitoring of coastal dynamics is expected that managers and public authorities can articulate multidisciplinary work plans, always aiming actions that seek understanding and effective commitment to the recovery of the harmonious interaction between society and Ponta Negra beach environment

Aplicação da tecnologia eletroquímica para a remoção e monitoramento de metais pesados em efluentes aquosos

Heavy metals are present in industrial waste. These metals can generate a large environmental impact contaminating water, soil and plants. The chemical action of heavy metals has attracted environmental interest. In this context, this study aimed to test t he performance of electrochemical technologies for removing and quantifying heavy metals. First ly , the electroanalytical technique of stripping voltammetry with glassy carbon electrode (GC) was standardized in order to use this method for the quantificatio n of metals during their removal by electrocoagulation process (EC). A nalytical curves were evaluated to obtain reliability of the determin ation and quantification of Cd 2+ and Pb 2+ separately or in a mixture. Meanwhile , EC process was developed using an el ectrochemical cell in a continuous flow (EFC) for removing Pb 2+ and Cd 2+ . The se experiments were performed using Al parallel plates with 10 cm of diameter (  63.5 cm 2 ) . The optimization of conditions for removing Pb 2+ and Cd 2+ , dissolved in 2 L of solution at 151 L h - 1 , were studied by applying different values of current for 30 min. Cd 2+ and Pb 2+ concentrations were monitored during electrolysis using stripping voltammetry. The results showed that the removal of Pb 2 + was effective when the EC pro cess is used, obtaining removals of 98% in 30 min. This behavior is dependent on the applied current, which implies an increase in power consumption. From the results also verified that the stripping voltammetry technique is quite reliable deter mining Pb 2+ concentration , when compared with the measurements obtained by atomic absorption method (AA). In view of this, t he second objective of this study was to evaluate the removal of Cd 2+ and Pb 2+ (mixture solution) by EC . Removal efficiency increasing current was confirmed when 93% and 100% of Cd 2+ and Pb 2+ was removed after 30 min . The increase in the current promotes the oxidation of sacrificial electrodes, and consequently increased amount of coagulant, which influences the removal of heavy metals in solution. Adsortive voltammetry is a fast, reliable, economical and simple way to determine Cd 2+ and Pb 2+ during their removal. I t is more economical than those normally used, which require the use of toxic and expensive reagents. Our results demonstrated the potential use of electroanalytical techniques to monitor the course of environmental interventions. Thus, the application of the two techniques associated can be a reliable way to monitor environmental impacts due to the pollution of aquatic ecosystems by heavy metals.