Análise imuno-histoquímica das proteínas RANK, RANKL e OPG em dentes de ratos reimplantados

The RANK / RANKL / OPG sy stem plays an important role in bone formation and resorption . This finding has been regarded as one of the m ost important advances in the understanding of bone biology with respect to osteoclastogenesis. The aim of this study was to investigate the expression of RANKL / RANK / OPG markers in reimplanted t eeth of rats, and to observe the relationship between the expression of these markers and to oth and bone resorption. Thirty male Wistar rats (Rattus norvegicus albinos) had their maxillary right incisors extrac ted , and were divided into 2 groups according to the period that the extracted teeth were kept in dry air before reimplantation : G1 (n = 15) - 5 minutes , and G2 (n = 15) - 60 minutes . After reimplantation, teeth were analyzed at intervals of 1, 3 and 7 da ys. After these experimental periods, the animals were euthanized. Longitudinal sections with 5μm thick were obtained and stained with Hematoxylin and Eosin for histological analysis , while 3μm thick sections were subjected to immunohistochemical analysis of OPG , RANK and RANKL. The results showed that the RANK / RANKL / OPG system actively participates in both the repair process, as well as tooth and bone resorption . Extr a - alveolar time of 60 minutes before replantation caused minor expressions of RANKL a nd OPG, not influencing the expression of RANK; RANKL immunostaining showed higher in both groups when compared to other biomarkers, participating in all phases of bone and tooth resorption; RANKL was associated to both osteoclastogenesis and c ell ular proliferation , and was expressed in both groups.

Análise da imuno expressão das proteínas IL-17, IL-23 E RORγt na patogenia da doença periodontal

Periodontal disease is a chronic inflammatory condition primarily caused by bacteria in dental biofilm, which interact with the host, thus determining the nature of the resulting disease. Despite the wide knowledge about the pathogenesis of these diseases, the exact composition of the T cell profile during the active phase of the disease (Th1, Th2 or Th17) remains unknown. This study aimed to evaluate by immunohistochemical expression, the presence of the markers (IL-17, IL-23 and RORγt), involved in Th17 response in clinically healthy gingiva cases (n = 32), biofilm-induced gingivitis (n = 30), chronic periodontitis (n = 32) and aggressive periodontitis (n = 25), in order to analyze if the expression and/or distribution of these molecules in lymphocytes and macrophages, present in the inflammatory infiltrate of periodontal tissue, influences the tissue destruction observed in these diseases. The morphological analysis of cases was performed which assessed the intensity of the inflammatory infiltrate in mild, moderate and intense. For each case, in the area with the most representative immunostaining, 5 fields were chosen and analyzed, both for the intensity of the inflammatory infiltrate as for the quantity of immunostained cells, based on predetermined scores: score 0 (absence of inflammatory infiltrate/immunostaining), score 1 (the infiltrate/immunostaining covered less than 25% of the field area), score 2 (the infiltrate/immunostaining occupied between 25 and 50%) and score 3 (infiltrate/immunostaining present in over 50% of the field area). From this, a median was generated representing each case. The intensity of the inflammatory infiltrate correlated with the disease progression, in other words, it was crescent from clinically healthy gingiva to aggressive periodontitis (P <0.001). It was detected the presence of IL-17, IL-23 and RORγt in most of the evaluated cases and the number of immunostained cells correlated with the intensity of the inflammatory infiltrate (P <0.001) and with the clinical parameters analyzed (P <0.001), showing a positive correlation, mainly moderate. Aggressive periodontitis showed a higher percentage of immunostaining for all markers in relation to other clinical conditions assessed, suggesting a possible association of these markers with the progression of this disease, in which the higher the loss of periodontal support, the greater the amount of inflammatory infiltrate and larger the number of immunostained cells.

A presença de homens docentes na educação infantil: lugares (des)ocupados

A partir da abordagem investigativa da multirreferencialidade, com base em Jacques Ardoíno, e tendo como lentes teóricas os conceitos de cultura e de gênero, discutidos respectivamente por Victor Hell, Stuart Hall, Alfredo Veiga-Neto, e Joan Scott, Guacira Lopes Louro, Dagmar Meyer, este estudo tem como objetivo analisar as implicações da presença de homens docentes na Educação Infantil, problematizando as relações de gênero a partir de um amálgama de atravessamentos sociais, históricos e culturais. Para tanto, foram realizadas entrevistas semi-estruturadas com dois homens que exercem a função de educador infantil na rede pública de ensino do Município de Natal/RN/Brasil. A análise das entrevistas possibilitou a organização dos dados em quatro focos de discussão. O primeiro deles refere-se às figuras masculinas (re)produzidas no cotidiano da Educação Infantil, cujas representações discursivas constroem o homem como “perigoso”, “poderoso” e “respeitado”, ajudando a perpetuar os vínculos histórica e culturalmente estabelecidos entre a figura masculina e as imagens de autoridade, liderança, comando, ao mesmo tempo em que reforça o suposto caráter natural das relações entre a figura feminina e o cuidado infantil. O segundo foco de discussão trata da polarização entre os elementos do binômio masculino/feminino, ativado constantemente nas relações que se estabelecem entre homens e mulheres no cotidiano da Educação Infantil, tornando as instituições desta etapa de ensino um local de concretização dos mais diversos significados de gênero. O terceiro foco de discussão problematiza a necessidade de afirmação da heterossexualidade dos educadores infantis entrevistados, destacando-se a associação (geralmente feita por gestores, por colegas de trabalho e pelas famílias das crianças) entre a heterossexualidade masculina e o adequado exercício da função docente. O quarto e último foco de discussão trata das lacunas da formação inicial e continuada de educadores infantis, enfatizando que, de modo geral, os cursos que formam professores para atuar em instituições de Educação Infantil tendem a desconsiderar a inserção profissional de homens nessa etapa de ensino. Assim, a Dissertação que ora apresentamos tem como escopo a desnaturalização de estereótipos de gênero no cotidiano da Educação Infantil, entendendo que este é também um contexto muito profícuo para o exercício de resistência às imagens cultural e historicamente associadas a homens e mulheres.

Viver e esperar viver: corpo e identidade na transição de gênero de homens trans

This work seeks to understand how trans men build their identities and live the transsexual experience in the relationships they establish daily onto “man” category. It could be observed that for it they engenders a specific gender transition in the midst of male transsexuality. Despite being under a complex amalgam of relations of exploitation and disciplinary domination, ways of being man are brokered for a living and entry into spaces where they are expelled for not conform the bodies that gender norms require. It is understood that gender transition is a process at the same time of organic and prosthetic body management and the assumption of your own identity. Thus, they build a politic of identity that creatively fixes a person's category as rights holder. The "transition" is therefore to transact from nonexistence to a place of humanity. This dissertation describes how this process takes place in the experiences of the speakers, observing the practices that bring out the male, front of class positions on the labor market, access to health, hormonization and own identity. Thereby, theories that fix them as expressing female masculinities or marginal to the hegemony do not find exactitude in their lives. The research methodologically started performing "multilocated ethnographies" that gave possibilities to in-depth interviews with 15 stakeholders from the Northeast, Midwest, Southeast and South of Brazil. Between 2014 and 2015, from the applying of network technique to the first dialogues in research, it was possible to build a participant observation by the trans men’s everyday life. Wherewith I was capable to behold their own private activities, as well as their public agency amid a trans activism collective in northeast, and the follow-up actions in which they were involved during the XII Encontro Nacional em Universidades de Diversidade Sexual e de Gênero (ENUDSG) held in Mossoró/RN. Therefore, the thesis engages to describe and understand the different ways of constructing trans male gender transitions in access to transsexuality and therefore a way of explaining their own trajectories in terms of people that exist as such, even though in the midst of narratives marked by emotions linked to "not live", to suffering and dehumanization.

Avaliação do planejamento de prótese parcial removível em modelos recebidos por laboratórios de João Pessoa-PB

Objective: To evaluate the planning and quality of plaster models for fabricati on of removable parti al dentures received from three commercial prosthodonti c laboratories located in the city of João Pessoa, PB, Brazil, which perform the casting procedures in their facilities. Methods: Forty 40 plaster models were photographed per laboratory, totalizing 120 models. The evaluation was performed using two questi onnaires, one designed for the dental prosthesis technicians, and another applied by the investigator for the visual evaluati on of the models. Data were analyzed using the SPSS soft ware version 13.0. Results: Ninety-two (76.7%) models did not present planning. In addition, no model presented references of insertion plane or guide pins. Calculati on of the mouth preparati on index (MPI) to evaluate the distribution of the oclusal and cingulum abutments or rests showed that 86 (71.7%) models were classifi ed as poor, 23 (19.2%) models as good and only 11 (9,.2%) models as acceptable. Defects were found in 102 (85%) models. Conclusion: The prosthodontists are not preparing the mouth of their pati ents, neglecti ng the planning of removable partial dentures, and passing this responsibility to the dental prosthesis technicians. In addition, the quality of the models sent to the laboratories was unsatisfactory.

Avaliação do planejamento de prótese parcial removível em modelos recebidos por laboratórios de João Pessoa-PB

Objective: To evaluate the planning and quality of plaster models for fabricati on of removable parti al dentures received from three commercial prosthodonti c laboratories located in the city of João Pessoa, PB, Brazil, which perform the casting procedures in their facilities. Methods: Forty 40 plaster models were photographed per laboratory, totalizing 120 models. The evaluation was performed using two questi onnaires, one designed for the dental prosthesis technicians, and another applied by the investigator for the visual evaluati on of the models. Data were analyzed using the SPSS soft ware version 13.0. Results: Ninety-two (76.7%) models did not present planning. In addition, no model presented references of insertion plane or guide pins. Calculati on of the mouth preparati on index (MPI) to evaluate the distribution of the oclusal and cingulum abutments or rests showed that 86 (71.7%) models were classifi ed as poor, 23 (19.2%) models as good and only 11 (9,.2%) models as acceptable. Defects were found in 102 (85%) models. Conclusion: The prosthodontists are not preparing the mouth of their pati ents, neglecti ng the planning of removable partial dentures, and passing this responsibility to the dental prosthesis technicians. In addition, the quality of the models sent to the laboratories was unsatisfactory.

Aspectos metodológicos do Projeto SBBrasil 2010 de interesse para inquéritos nacionais de saúde

The SBBrasil 2010 Project (SBB10) was designed as a nationwide oral health epidemiological survey within a health surveillance strategy. This article discusses methodological aspects of the SBB10 Project that can potentially help expand and develop knowledge in the health field. This was a nationwide survey with stratified multi-stage cluster sampling. The sample domains were 27 State capitals and 150 rural municipalities (counties) from the country's five major geographic regions. The sampling units were census tracts and households for the State capitals and municipalities, census tracts, and households for the rural areas. Thirty census tracts were selected in the State capitals and 30 municipalities in the countryside. The precision considered the demographic domains grouped by density of the overall population and the internal variability of oral health indices. The study evaluated dental caries, periodontal disease, malocclusion, fluorosis, tooth loss, and dental trauma in five age groups (5, 12, 15-19, 35-44, and 65-74 years).

Aspectos metodológicos do Projeto SBBrasil 2010 de interesse para inquéritos nacionais de saúde

The SBBrasil 2010 Project (SBB10) was designed as a nationwide oral health epidemiological survey within a health surveillance strategy. This article discusses methodological aspects of the SBB10 Project that can potentially help expand and develop knowledge in the health field. This was a nationwide survey with stratified multi-stage cluster sampling. The sample domains were 27 State capitals and 150 rural municipalities (counties) from the country's five major geographic regions. The sampling units were census tracts and households for the State capitals and municipalities, census tracts, and households for the rural areas. Thirty census tracts were selected in the State capitals and 30 municipalities in the countryside. The precision considered the demographic domains grouped by density of the overall population and the internal variability of oral health indices. The study evaluated dental caries, periodontal disease, malocclusion, fluorosis, tooth loss, and dental trauma in five age groups (5, 12, 15-19, 35-44, and 65-74 years).

Rugosidade superficial e adesão bacteriana em compósitos com nanopartículas após acabamento e polimento

Objective: To evaluate in vitro the surface roughness and bacterial adhesion of nanoparticle composites, after being subjected to different finishing and polishing systems. Materials and Methods: 66 specimens were prepared, and 30 with Filtek Z350 XT (3M ESPE, USA) and 30 with the resin IPS Empress Direct (Ivoclar Vivadent, USA), divided into 6 groups (n = 10 ). Six specimens were prepared for analysis in scanning electron microscopy (SEM) .Each kind of resin was subjected to finishing and polishing systems: Sof-Lex Pop-On discs (3M ESPE, USA) and AstropolTM system (Ivoclar Vivadent , USA), featuring the experimental group. The control group did not undergo any kind of finishing and polishing technique. The average roughness (Ra) in both groups was measured using a roughness in the setting of 0.25 mm (cut off) and surface images obtained with photomicrographs taken with a scanning electron microscope (SEM) magnified 500 times. Bacterial adherence was evaluated by determining the absorbance (OD) of the suspension of adhered cells by spectrophotometer at 570 nm. The results were submitted for analyzed with 2-way ANOVA at α=.05 and Tukey multiple comparison tests. Results: Statistically significant differences were found between the groups in terms of roughness and bacterial adhesion. Filtek Z350 XT for resin were no differences between the tested finishing and polishing systems, where the system of lowest surface roughness was the Sof-Lex Pop-On. To the resin IPS Empress Direct, the finishing and polishing system Astropol, had lower results of surface roughness. As for bacterial adhesion, the lowest optical density value for Filtek Z350 XT was for the group that used the finishing and polishing system Sof-Lex Pop-On and the resin IPS Empress Direct the group that used the Astropol system. In addition, there was a positive correlation between surface roughness and bacterial adhesion on polished surfaces (r = 0.612) Conclusions: surface roughness and bacterial adhesion are closely related. The finishing and polishing Sof-Lex Pop-On system is more suitable for nanoparticulate Filtek Z350 XT and the finishing and polishing system Astropol for resin nanohíbrida IPS Empress Direct.