Caracterização da resistência do algodoeiro a Ramularia areola e variabilidade molecular do patógeno

This research was conducted with the aim to study the genetic and pathogenic structure of Ramularia areola isolates collected in Brazil and to characterize the resistance response in cotton plants to ramularia spot. The genetic variability of 28 isolates of R. areola was studied using RAPD markers. The pathogenicity evaluation was realized by the inoculation of 6 isolates on cotton varieties Guazuncho-2 (Gossypium hirsutum) and VH8-4602 (Gossypium barbadense). The inheritance of disease resistance was studied using an artificially inoculated population of F2 individuals derived from the intercross of Guazuncho-2 (susceptible variety) end VH8-4602 (resistant variety), and also the parents and F1 individuals. Molecular polymorphism between the G. hisutum varieties DeltaOpal (suscetible) and CNPA CO-11612 (resistant) was estimated by 118 SSR and 24 AFLP markers. The parental genotypes Guazuncho-2 and VH8-4602 were selected for mapping, and then Recombinant Inbred Lines (RIL´s) derived from this crossing were evaluated with SSR 12 markers. The analysis of population structure of R. areola revealed that the three subpopulations were genetically simillar (Gst=0.18), and the isolates from Goiás and Minas Gerais were more similar to each other (0,92). This probability can be related to the relatively high gene flow among the three subpopulations (Nm=2.20). The isolates R. areola 9.1, from Minas Gerais State and 8.1 and 8.3 from Goiás State were the most aggressive ones to the susceptible variety Guazuncho-2. The variety VH8-4602 presented high level of resistance to ramularia spot. No differential interaction was observed between the pathogens and the analyzed varieties, and the resistance was classified as horizontal. The quantification of disease by number of necrotic lesions and number of spores in individual plants of F1 and F2 generations from the crossing between the varieties Guazuncho-2 and VH8-4602 presented continuous distribution, suggesting polygenic resistance. The resistance is probabilly recessive, since necrotic lesions and sporulation were observed on F1 plants. The molecular polymorphism between DeltaOpal e CNPA CO-11612 lineages was low (6%), then would be difficult to accomplish molecular mapping of disease resistance using this intercross. With the genotyping of the RIL s it was verified that 25% of the markers segregated in the proportions proposed by Mendel s Law and 75% of the studied markers presented segregation distortion in favor to the parental G. hirsutum. Both the low genetic variability of the pathogen and the number of resistance genes suggest that durable genetic resitance may be achieved

Uma abordagem anotativa para gerência de variabilidade em linhas de processos de software: concepção, implementação e avaliação

Nowadays, the importance of using software processes is already consolidated and is considered fundamental to the success of software development projects. Large and medium software projects demand the definition and continuous improvement of software processes in order to promote the productive development of high-quality software. Customizing and evolving existing software processes to address the variety of scenarios, technologies, culture and scale is a recurrent challenge required by the software industry. It involves the adaptation of software process models for the reality of their projects. Besides, it must also promote the reuse of past experiences in the definition and development of software processes for the new projects. The adequate management and execution of software processes can bring a better quality and productivity to the produced software systems. This work aimed to explore the use and adaptation of consolidated software product lines techniques to promote the management of the variabilities of software process families. In order to achieve this aim: (i) a systematic literature review is conducted to identify and characterize variability management approaches for software processes; (ii) an annotative approach for the variability management of software process lines is proposed and developed; and finally (iii) empirical studies and a controlled experiment assess and compare the proposed annotative approach against a compositional one. One study a comparative qualitative study analyzed the annotative and compositional approaches from different perspectives, such as: modularity, traceability, error detection, granularity, uniformity, adoption, and systematic variability management. Another study a comparative quantitative study has considered internal attributes of the specification of software process lines, such as modularity, size and complexity. Finally, the last study a controlled experiment evaluated the effort to use and the understandability of the investigated approaches when modeling and evolving specifications of software process lines. The studies bring evidences of several benefits of the annotative approach, and the potential of integration with the compositional approach, to assist the variability management of software process lines

Uma abordagem dirigida por modelos para gerência de variabilidade e execução de processos de software

This dissertation presents a model-driven and integrated approach to variability management, customization and execution of software processes. Our approach is founded on the principles and techniques of software product lines and model-driven engineering. Model-driven engineering provides support to the specification of software processes and their transformation to workflow specifications. Software product lines techniques allows the automatic variability management of process elements and fragments. Additionally, in our approach, workflow technologies enable the process execution in workflow engines. In order to evaluate the approach feasibility, we have implemented it using existing model-driven engineering technologies. The software processes are specified using Eclipse Process Framework (EPF). The automatic variability management of software processes has been implemented as an extension of an existing product derivation tool. Finally, ATL and Acceleo transformation languages are adopted to transform EPF process to jPDL workflow language specifications in order to enable the deployment and execution of software processes in the JBoss BPM workflow engine. The approach is evaluated through the modeling and modularization of the project management discipline of the Open Unified Process (OpenUP)