An��lise da Express��o de APE1 ap��s Estresse Oxidativo Induzido em C��lulas Proficientes e Deficientes na Via de Reparo por Excis��o de Nucleot��deos

Riboflavin is a vitamin very important in aerobic organisms, as a precursor of many coenzymes involved in the electron transporter chain. However, after photosensitization of riboflavin with UV or visible light, it generates reactive oxygen species (ROS), which can oxidize the DNA. The repair of oxidative lesions on DNA occurs through the base excision repair pathway (BER), where APE1 endonuclease plays a central role. On the other hand, the nucleotide excision repair pathway (NER) repairs helix-distorting lesions. Recently, it was described the participation of NERproteins in the repair of oxidative damage and in stimulation of repair function fromAPE1. The aim of this research was to evaluate the cytotoxic effects of photosensitized riboflavin (RF*) in cells proficient and deficient in NER, correlating with APE1 expression. For this propose, the cells were treated with RF* and it was performed the cell viability assay, extraction of whole proteins, cells fractionation, immunoblotting, indirect immunofluorescence and analysis of polymorphisms of BER gens. The results evidenced that cells deficient in XPA and CSB proteins were more sensitive to RF*. However, XPC-deficient cells presented similar resistance to MRC5- SV cells, which is proficient in NER. These results indicate that XPA and CSB proteins have an important role on repair of oxidative lesions induced by RF*. Additionally, it was evidenced that single nucleotide polymorphisms (SNPs) in BER enzymes may influence in sensitivity of NER-deficient cell lines. Concerning the APE1 expression, the results showed that expression of this protein after treatment with RF* only changed in XPC-deficient cells. Though, it was observed that APE1 is recruited and is bound to chromatin in MRC5-SV and XPA cells after treatment with RF*. The results also showed the induction of DNA damage after treatment with RF*, through the analysis of-H2AX, since the treatment promoted an increase of endogenous levels of this phosphorylated protein, which acts signaling double strand-break on DNA. On the other hand, in XPC-deficient cells, regardless of resistance of RF*, the endogenous levels of APE1 are extremely reduced when compared with other cell lines and APE1 is not bound to chromatin after treatment with RF*. These results conclude that RF* was able to induce cell death in NERdeficient cells, where XPA and CSB cells were more sensitive when compared with MRC5-SV and XPC-deficient cells. This last result is potentially very interesting, since XPC-deficient cell line presents low levels of APE1. Additionally, the results evidenced that APE1 protein can be involved in the repair of oxidative damage induced by RF*, because APE1 is recruited and bound strongly to chromatin after treatment.

Efeitos da sintomatologia depressiva na mobilidade de idosos de um centro urbano do nordeste brasileiro

O processo de envelhecimento da humanidade �� um fen��meno mundial e nas ��ltimas d��cadas a popula����o brasileira tem acompanhado essa tend��ncia. No entanto, este fen��meno somente pode ser considerado como uma real conquista em termos s��cios demogr��ficos na medida em que se agregue qualidade aos anos adicionais de vida para a popula����o. Em popula����es idosas, a incapacidade funcional e os sintomas depressivos tornaram-se importantes conceitos tanto por suas consequ��ncias para a sa��de p��blica, quanto pelo impacto na qualidade de vida dessas popula����es. Este estudo tem como objetivo verificar se h�� associa����o entre os sintomas depressivos e o desempenho funcional de idosos comunit��rios. Foram avaliados 313 idosos de um centro urbano do nordeste brasileiro atrav��s das baterias de testes; Center for Epidemiologic Studies Depression CES-D e Short Physical Performance Battery - SPPB; al��m de vari��veis relacionadas ao estado f��sico, condi����es cr��nicas, fun����o cognitiva e caracter��sticas s��cio demogr��ficas. Foram utilizadas medidas de correla����o e aplicado m��todo de an��lise multivariada para construir um modelo explicativo da influ��ncia da sintomatologia depressiva no desempenho funcional. Os resultados revelaram que as mulheres apresentaram mais sintomas depressivos (p< 0.01) e pior desempenho funcional (p< 0.01) que os homens. Os indiv��duos com sintomatologia depressiva apresentaram pior desempenho funcional, especialmente as mulheres. A idade, o sexo feminino, o estado de sa��de percebido, o n��vel de fun����o cognitiva e o ��ndice de massa corporal foram identificados como fatores associados ao desempenho funcional na amostra estudada. Os resultados deste estudo permitem inferir a rela����o de associa����o entre sintomas depressivos e desempenho funcional em uma popula����o de idosos de um centro urbano no nordeste do Brasil. Estes resultados fornecem informa����es ��teis para interven����es terap��uticas adequadas voltadas para a preven����o do decl��nio na mobilidade em idosos

An��lise cr��tica dos produtos de mobilidade sentada - cadeiras de rodas - utilizados por crian��as e adolescentes com paralisia cerebral em Natal/RN e outros munic��pios do Rio Grande do Norte

The objective of this work is to critically analyze the seated mobility adaptive equipment, wheelchair, used by non-ambulatory children/adolescents diagnosed with cerebral palsy of the quadriplegy type with ages in between 0-18 years old in the municipal city of Natal/RN and of other ones in the state of Rio Grande do Norte. This study is characterized as descriptive as it took into consideration the individualized conditions of each child/adolescent and of their home environment, and also evaluates the condition of the used seated mobility devices such as wheelchairs or strollers. Thirty three (33) subjects which had a wheelchair prescribed by a healthcare professional specialist in adaptive equipment within the past 3 years were selected. A questionnaire was applied through a phone with the patient s primary care giver or parents. The results obtained showed that all the equipment being used had an anatomical seat and back and the tilt system mechanism and even though most of the patients required small individual adjustments upon receiving the wheelchair in order to accommodate to the patients characteristics. From the point of view of the caregiver/parents interviewed, 97% described satisfied with the equipment and report assistance with mobility (97%), comfort (82%) and good posture (82%) as the main positive aspects from the equipment. The results also describe the aspects related to the maintenance, time used and activities developed that were reported by the patients. The conclusion therefore is that in order to select the equipment to meet the needs of the consumer it is necessary that: an appropriate wheelchair prescription takes place, the up-to-date knowledge of equipment available in the market, the appropriate use of the resources from the concession programs of the government that purchase equipment and the establishment of link in between the manufacturers, patients and the caregivers

Otimiza����es da transmiss��o de imagens em redes de sensores visuais sem fio explorando a relev��ncia de monitoramento dos n��s fontes e codifica����o DWT

The development of wireless sensor networks for control and monitoring functions has created a vibrant investigation scenario, covering since communication aspects to issues related with energy efficiency. When source sensors are endowed with cameras for visual monitoring, a new scope of challenges is raised, as transmission and monitoring requirements are considerably changed. Particularly, visual sensors collect data following a directional sensing model, altering the meaning of concepts as vicinity and redundancy but allowing the differentiation of source nodes by their sensing relevancies for the application. In such context, we propose the combined use of two differentiation strategies as a novel QoS parameter, exploring the sensing relevancies of source nodes and DWT image coding. This innovative approach supports a new scope of optimizations to improve the performance of visual sensor networks at the cost of a small reduction on the overall monitoring quality of the application. Besides definition of a new concept of relevance and the proposition of mechanisms to support its practical exploitation, we propose five different optimizations in the way images are transmitted in wireless visual sensor networks, aiming at energy saving, transmission with low delay and error recovery. Putting all these together, the proposed innovative differentiation strategies and the related optimizations open a relevant research trend, where the application monitoring requirements are used to guide a more efficient operation of sensor networks

Odometria visual baseada em t��cnicas de structure from motion

Visual Odometry is the process that estimates camera position and orientation based solely on images and in features (projections of visual landmarks present in the scene) extraced from them. With the increasing advance of Computer Vision algorithms and computer processing power, the subarea known as Structure from Motion (SFM) started to supply mathematical tools composing localization systems for robotics and Augmented Reality applications, in contrast with its initial purpose of being used in inherently offline solutions aiming 3D reconstruction and image based modelling. In that way, this work proposes a pipeline to obtain relative position featuring a previously calibrated camera as positional sensor and based entirely on models and algorithms from SFM. Techniques usually applied in camera localization systems such as Kalman filters and particle filters are not used, making unnecessary additional information like probabilistic models for camera state transition. Experiments assessing both 3D reconstruction quality and camera position estimated by the system were performed, in which image sequences captured in reallistic scenarios were processed and compared to localization data gathered from a mobile robotic platform

T��cnicas visuais de localiza����o e mapeamento simult��neos sem extra����o de primitivas geom��tricas da imagem

In Simultaneous Localization and Mapping (SLAM - Simultaneous Localization and Mapping), a robot placed in an unknown location in any environment must be able to create a perspective of this environment (a map) and is situated in the same simultaneously, using only information captured by the robot s sensors and control signals known. Recently, driven by the advance of computing power, work in this area have proposed to use video camera as a sensor and it came so Visual SLAM. This has several approaches and the vast majority of them work basically extracting features of the environment, calculating the necessary correspondence and through these estimate the required parameters. This work presented a monocular visual SLAM system that uses direct image registration to calculate the image reprojection error and optimization methods that minimize this error and thus obtain the parameters for the robot pose and map of the environment directly from the pixels of the images. Thus the steps of extracting and matching features are not needed, enabling our system works well in environments where traditional approaches have difficulty. Moreover, when addressing the problem of SLAM as proposed in this work we avoid a very common problem in traditional approaches, known as error propagation. Worrying about the high computational cost of this approach have been tested several types of optimization methods in order to find a good balance between good estimates and processing time. The results presented in this work show the success of this system in different environments

Controle de posi����o com restri����o �� orientação de um Ve��culo A��reo N��o-Tripulado tipo Quadrirrotor

Quadrotors aircraft are composed by four propellers mounted on four engines on a cross or x disposition, and, in this structure, the engines on the same arm spin in the same direction and the other arm in the opposite direction. By rotating each helix generates vertical upward thrust. The control is done by varying the rotational speed of each motor. Among the advantages of this type of vehicle can cite the mechanical simplicity of construction, the high degree of maneuverability and the ability to have vertical takeoffs and landings. The modeling and control of quadrirrotores have been a challenge due to problems such as nonlinearity and coupling between variables. Several strategies have been developed to control this type of vehicle, from the classical control to modern. There are air surveillance applications where a camera is fixed on the vehicle to point forward, where it is desired that the quadrotor moves at a fixed altitude toward the target also pointing forward, which imposes an artificial constraint motion, because it is not desired that it moves laterally, but only forwards or backwards and around its axes . This restriction is similar to the naturally existing on robots powered by wheels with differential drive, which also can not move laterally, due to the friction of the wheels. Therefore, a position control strategy similar to that used in this type of robot could be adapted for aerial robots like quadrotor. This dissertation presents and discusses some strategies for the control of position and orientation of quadrotors found in the literature and proposes a strategy based on dynamic control of mobile robots with differential drive, called the variable reference control. The validity of the proposed strategy is demonstrated through computer simulations

A ret��rica em Arist��teles: da orientação das paix��es ao aprimoramento da eupraxia

This research brings into focus the relationship between the work Rhetoric, from Aristotle, and the conceptions of ethics and practical wisdom of the philosopher from Stageira. Accordingly, it attempts to show that Aristotle's Rhetoric was produced to guide the construction and orientation of oratory passions of the Greek man, setting it as a reference for practices aimed at social ordering of the polis. In other words, the Aristotelian Rhetoric, designed by the author as the study of what is persuasive in every speech, is not composed with the meaning of persuasion at any cost, in another sense it is conceived by Aristotle as a useful knowledge for the improvement eupraxic (the good act in accordance with the fair and true). This research finds that such work has been prepared by Stagirite a time of strong social transformations and upheavals in ancient Greece: The skepticism expanded, with each person wanting to live their own businesses, and especially in Athens, a city that served as intellectual and political reference, there was a lack of collective spirit. In this tumultuous social environment, Aristotle, with a culture of Greeks eager Trusted reviews and socially shareable in the field of verisimilitude, sought with his Rhetoric, contributing to the development of ethics and political science; referrals for legal and organization of inter-social relations in varied environments, including seeking to provide knowledge about human passions and emotional status of active citizens in deliberative meetings

Grau de conhecimento de pacientes sobre o acidente vascular cerebral e a necessidade do uso de cartilha educativa para orientação fisioterap��utica

Stroke is a neurological dysfunction of vascular origin that causes physical impairments and disabilities. Brazil leads the statistics stroke mortality among Latin American countries, demonstrating still be a neglected disease in this country. The incidence is related to risk factors and still is great misinformation in our country about stroke, treatment and prophylaxis. The aim of this study was to assess the degree of knowledge about stroke and patients need to use educational booklet for physiotherapy. The sample consisted of 53 patients, 22 females and 31 males, mean age 56.2 �� 10.9 years. Patients were evaluated by completing questionnaires, observing the socio-demographic and clinical aspects, neurological assessment, functional assessment, knowledge of pathology and presenting educational booklet. The data were analyzed using the chi-square test. According to the results, it was observed that the patients had not enough knowledge about the terminology, complications and warning signs of stroke, were not aware and did not perform the proper positioning of the limb and transfers, however, were aware and performed the stretching maneuvers. This reveals the need to implement policies that lead to information about stroke population. Following discharge from outpatient physical therapy using educational booklets with guidelines on positioning, stretching exercises and transfer is important in functional rehabilitation and encouraging independence of the individual affected by stroke

Compara����o e mobilidade, equil��brio corporal e desempenho muscular segundo a auto-efic��cia para quedas em idosas

The aging process modifies various systems in the body, leading to changes in mobility, balance and muscle strength. This can cause a drop in the elderly, or not changing the perceived self-efficacy in preventing falls. Objective: To compare the mobility, body balance and muscle performance according to self-efficacy for falls in community-dwelling elderly. Methods: A cross-sectional comparative study with 63 older (65-80 years) community. Were evaluated for identification data and sociodemographic, cognitive screening using the Mini Mental State Examination (MMSE), effective for the fall of Falls Efficacy Scale International Brazil (FES-I-BRAZIL), Mobility through the Timed Up and Go Test , the balance Berg Balance Scale (BBS) and the Modified Clinical Test tests of Sensory Interaction on Balance (mCTSIB), tandem walk (TW) and Sit to Stand (STS) of the Balance Master�� System. Finally, muscle performance by using isokinetic dynamometry. Statistical analysis was performed Student t test for comparison between groups, with p value &#8804; 0.05. Results: Comparing the elderly with low-efficacy for falls with high-efficacy for falls, we found significant differences only for the variable Timed Up and Go Test (p = 0.04). With regard to data on balance tests were significant differences in the speed of oscillation firm surface eyes open modified Clinical Test of Sensory Interaction on Test of Balance (p = 0.01). Variables to isokinetic dynamometry were no significant differences in movement knee extension, as regards the variables peak torque (p = 0.04) and power (p = 0.03). Conclusion: The results suggest that, compared to older community with low-and high-efficacy for falls, we observed differences in variables related to mobility, balance and muscle function

The influence of visual inter-hemispheric connections on spiking, assembly and LFP activities, and their phase relationship during figure-ground stimulation

Desde os descobrimentos pioneiros de Hubel e Wiesel acumulou-se uma vasta literatura descrevendo as respostas neuronais do c��rtex visual prim��rio (V1) a diferentes est��mulos visuais. Estes est��mulos consistem principalmente em barras em movimento, pontos ou grades, que s��o ��teis para explorar as respostas dentro do campo receptivo cl��ssico (CRF do ingl��s classical receptive field) a caracter��sticas b��sicas dos est��mulos visuais como a orientação, dire����o de movimento, contraste, entre outras. Entretanto, nas ��ltimas duas d��cadas, tornou-se cada vez mais evidente que a atividade de neur��nios em V1 pode ser modulada por est��mulos fora do CRF. Desta forma, ��reas visuais prim��rias poderiam estar envolvidas em fun����es visuais mais complexas como, por exemplo, a separa����o de um objeto ou figura do seu fundo (segrega����o figura-fundo) e assume-se que as conex��es intr��nsecas de longo alcance em V1, assim como as conex��es de ��reas visuais superiores, est��o ativamente envolvidas neste processo. Sua poss��vel fun����o foi inferida a partir da an��lise das varia����es das respostas induzidas por um est��mulo localizado fora do CRF de neur��nios individuais. Mesmo sendo muito prov��vel que estas conex��es tenham tamb��m um impacto tanto na atividade conjunta de neur��nios envolvidos no processamento da figura quanto no potencial de campo, estas quest��es permanecem pouco estudadas. Visando examinar a modula����o do contexto visual nessas atividades, coletamos potenciais de a����o e potenciais de campo em paralelo de at�� 48 eletrodos implantados na ��rea visual prim��ria de gatos anestesiados. Estimulamos com grades compostas e cenas naturais, focando-nos na atividade de neur��nios cujo CRF estava situado na figura. Da mesma forma, visando examinar a influ��ncia das conex��es laterais, o sinal proveniente da ��rea visual isot��pica e contralateral foi removido atrav��s da desativa����o revers��vel por resfriamento. Fizemos isso devido a: i) as conex��es laterais intr��nsecas n��o podem ser facilmente manipuladas sem afetar diretamente os sinais que est��o sendo medidos, ii) as conex��es inter-hemisf��ricas compartilham as principais caracter��sticas anat��micas com a rede lateral intr��nseca e podem ser vistas como uma continua����o funcional das mesmas entre os dois hemisf��rios e iii) o resfriamento desativa as conex��es de forma causal e revers��vel, silenciando temporariamente seu sinal, permitindo conclus��es diretas a respeito da sua contribui����o. Nossos resultados demonstram que o mecanismo de segmenta����o figurafundo se reflete nas taxas de disparo de neur��nios individuais, assim como na pot��ncia do potencial de campo e na rela����o entre sua fase e os padr��es de disparo produzidos pela popula����o. Al��m disso, as conex��es laterais inter-hemisf��ricas modulam estas vari��veis dependendo da estimula����o feita fora do CRF. Observamos tamb��m uma influ��ncia deste circuito lateral na coer��ncia entre potenciais de campo entre eletrodos distantes. Em conclus��o, nossos resultados d��o suporte �� ideia de um mecanismo complexo de segmenta����o figura-fundo atuando desde as ��reas visuais prim��rias em diferentes escalas de frequ��ncia. Esse mecanismo parece envolver grupos de neur��nios ativos sincronicamente e dependentes da fase do potencial de campo. Nossos resultados tamb��m s��o compat��veis com a hip��tese que conex��es laterais de longo alcance tamb��m fazem parte deste mecanismo

Avalia����o cl��nica longitudinal do periodonto de dentes pilares e n��o pilares de pr��teses parciais remov��veis

The literature has shown a relation between periodontics and the removable partial denture (RPD), with progressive destruction observed in the support structures. The aim of this study was to clinically assess periodontal condition in users of removal partial denture (RPD), and compare right abutments teeth, indirect abutments and controls before installation and after 1 year, in addition to comparing tooth-supported and tooth mucosa-supported abutments. A total of 50 patients, 32 women and 18 men, mean age of 45 years, took part in the study. The patients were examined by a single examiner at prosthesis installation and after 3, 6, 9 and 12 months. The following were verified at each examination: Probe Depth (PD), Plaque Index (PI), Gingival Index (GI), the amount of Keratinized Mucosa (KM), Gingival Recession (GR) and Dental Mobility (DM); in addition patients received oral hygiene orientation, accompanied by prophylaxis, periodontal scaling and root planing (PSRP), when necessary. Analysis of Variance (ANOVA) with Tukey-Kramer post test was used to assess the dependent variables (PD, PI, KM, GR) of the three groups over time while Friedman s test was used for GI. To assess the outcomes of prosthesis type in the right abutment group, a confidence interval-based analysis was performed. The results showed that the control group was the least compromised in all the variables studied. With respect to development of the groups over time, it was verified that the measures for GR, PD, GI and KM increased from initial examination to 1 year of use in all the groups, but only PI showed a significant increase. There was a non-discriminatory low prevalence of dental mobility. The tooth mucosa-supported prosthesis had significantly higher values for GR, GI and PI and significantly lower ones for KM when compared to tooth-supported. Over time, both types of prostheses showed no significant differences from initial to final examination for the variables GR, PD, KM and GI, with PI significant only for tooth-supported. The results showed that the teeth most involved in RPD design had greater potential of periodontal damage, probably because of greater dental biofilm accumulation. Abutments elements adjacent to the free extremities had less favorable periodontal condition than those adjacent to interpolated spaces, but the use of RPD did not worsen the initial condition

Extens��es ao Projeto LVWNet: mobilidade, intera����o com equipamentos reais, comunica����o direta, e registro din��mico de n��s

Due to the constantly increasing use of wireless networks in domestic, business and industrial environments, new challenges have emerged. The prototyping of new protocols in these environments is typically restricted to simulation environments, where there is the need of double implementation, one in the simulation environment where an initial proof of concept is performed and the other one in a real environment. Also, if real environments are used, it is not trivial to create a testbed for high density wireless networks given the need to use various real equipment as well as attenuators and power reducers to try to reduce the physical space required to create these laboratories. In this context, LVWNet (Linux Virtual Wireless Network) project was originally designed to create completely virtual testbeds for IEEE 802.11 networks on the Linux operating system. This paper aims to extend the current project LVWNet, adding to it the features like the ability to interact with real wireless hardware, provides a initial mobility ability using the positioning of the nodes in a space coordinates environment based on meters, with loss calculations due to attenuation in free space, enables some scalability increase by creating an own protocol that allows the communication between nodes without an intermediate host and dynamic registration of nodes, allowing new nodes to be inserted into in already in operation network

An��lise da mobilidade urbana de Natal/RN a partir do uso de indicadores de sustentabilidade

In contemporary times, the urban mobility is presented as one of the main challenges of large and medium-sized Brazilian cities , it presents signs of unsustainability , above all due to the increase in motorized transport system , especially the individual . Thus , it is clear that mobility is considered of special interest for sustainable urban development , is facing a crisis that is reflected in negative externalities , particularly with regard to congestion , accidents , air pollution , social exclusion , among others. The complexity of these problems requires an integrated analysis based on the principles of sustainability . In this sense , trying to understand this problem and in the attempt to minimize its impacts, Costa (2008 ) proposed the Index of Sustainable Urban Mobility (I_SUM) with the goal of providing a methodology capable to assess relevant aspects of mobility , including social dimensions , political, economic and environmental . Given the above, was elected as the main objective of this study is to analyze urban mobility in Natal / RN from the perspective of sustainability . Like specific objectives will seek to analyze Natal��s urban mobility, using the I_SUM like a tool for both the city as a whole and for each of its four administrative regions; evaluate Natal s urban accessibility conditions and verify to what extent the access to urban services and equipment is facilitated or hindered because of the location and incomes of the population . For this, it was necessary to use bibliographic , documentary and field research . The index , which varies from 0.00 to 1.00, revealed for Natal an Global value of 0,510, and that refers to its administrative regions, it was found that there are very small percentage differences from the overall index calculated for the city . The performance of I_SUM by administrative region showed that although there are differences , the results were very similar , varying between 0,494 and 0,537. Particularly in relation to urban accessibility, it appears that Natal presents as an unequal city and this inequity , which is not only income is reflected in the issues related to accessibility and hence urban mobility

Orientação de falhas presentes em testemunhos de sondagens do campo de Xar��u/bacia do Cear��

A research project is being developed by PPGG/UFRN and PETROBRAS in the Xar��u Oil Field located in Cear�� Basin, Northeastern Brazil. The objective of the research is to characterize a fractured carbonate reservoir, the Trairi Limestone, in order to drill a borehole with two horizontal legs taking advantage of the natural fracture system to enhance the oil recovery. The present master thesis is part of this research and its contribution is to estimate fault orientation from unoriented cores, using the method proposed by Hesthammer & Henden (2000). In order to orient a fault cutting a bed observed in the core, the bed should be previously oriented. As additional constraint to orient the bed, we use regional bedding orientation obtained from structure maps of Trairi Limestone. Because the number of cores drilled from the Trairi Limestone was too small, we analyzed all cores from the field. As geologic constraint, we admit that all faults were formed as result of the South America and Africa separation, in the context of a regional dextral strike-slip fault formation. In this context, secondary faults are manly T and R faults according Riedel s classification. We analyzed 236.5 m of cores. The dip of bedding varies from 0o to 8o, being the most frequent value equal to 2o. We interpret this result as evidence that the deformation process was manly ruptil. 77 faults were identified in the cores. These faults strike manly to NW and NE with dips, in general, inside the interval 700 - 900. We suggest that the horizontal legs of the borehole should be oriented to NW and NE in order to improve the probability of intercepting open fractures and faults