Avaliação de métodos de análises para determinação da contagem de células somáticas no leite cru, mantido em tanque de resfriamento

We analyzed the quality of raw milk from eight dairy farms in Rio Grande do Norte stored in a cooling tank , in order to evaluate methods for determining somatic cell counts (SCC). The Somaticell® kit and a portable Direct Cell Counter (DCC) were compared with each other and with the MilkoScanTM FT+ (FOSS Denmark), which uses Fourier Transform Infrared (FTIR) spectroscopy). Direct cell counter data were processed for somatic cell scores (log-transformed somatic cell count) and analyzed with the SAS®, statistical package , Statistical Analysis System, (SAS, INSTITUTE, 1998). Comparison of means and correlation of somatic cell scores were conducted using Pearson s correlation coefficient and the Tukey Test at 1 %. No significant difference was observed for comparison of means. The correlation between somatic cell scores was significant, that is, 0.907 and 0.876 between the MilkoScanTM FT+ and the Somaticell® kit and Direct Cell Count (DCC) respectively, and 0.943 between the Somaticell® kit and Direct Cell Count (DCC). The methods can be recommended for monitoring the quality of raw milk kept in a cooling tank in the production unit

Marcadores fenotípicos para caracterização de caprinos com diferentes níveis de resistência às endoparasitoses gastrintestinais

The aim of this study was to evaluate and characterize phenotypically goats with different levels of resistance to gastrointestinal nematodes. For a period of 93 days, 60 F2 goats originated from ½ Saanen and ½ Anglo- nubian animals were kept in the same area of pasture. Every seven days, feces and blood were collected for eggs per gram counts of feces (EPG) and cultures of feces and to determinate the number of eosinophils, packed cell volume and total plasma protein, respectively. On the same day, the animals were weighed and submitted to body score condition and FAMACHA method to worm control. Based on the average of EPG, the twelve animals with the highest average (susceptible group) and the twelve animals with the lowest average of EPG (resistant group) were selected, slaughtered and necropsied to recovery, counting andparasites identification. The resistant animals present lower EPG mean (P <0.0001) and 4.7 folder less parasites than susceptible animals. The resistant group presented higher mean packed cell volume (26.48%) and total plasma protein (6.24 g / dl) than susceptible one (24,04% e 5,82g/dl, respectively). The average number of eosinophils was similar in both groups The Haemonchus sp. was the most prevalent in the culture of feces, followed by Trischostrongylus sp. and Oesophagostomum sp.. The counting of nematodes in the abomasum of susceptible group was higher than in resistant one. The species identified were H. contortus in abomasums and T. colubriformis in small intestine. It can be concluded that EPG, packed cell volume and total plasma protein were useful phenotypic markers to identify animals as resistant and susceptible to gastrointestinal nematodes infections

Avaliação dos impactos ambientais e sociais gerados pela utilização do kit Embrapa de ordenha manual na produção de leite de cabra

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Caracterização genética de vacas leiteiras por meio de marcadores moleculares e suas implicações na composição e qualidade do leite

The objective of this study was to identify DNA polymorphisms at the genes leptin, β-lactoglobulin and pituitary-specific transcription factor in three genetic groups of Holstein x Guzerat dairy cows and investigate the relationship between their genotypes and the composition and quality of milk of dairy cows. Samples were collected in August 2009, being 113 blood samples from lactating crossbred cows and 58 milk samples. For analysis of DNA polymorphisms blood samples were collected, analyzed later in the Genetic Laboratory affiliated to the Zootechny Institute of São Paulo and individual milk samples were collected according to standards established by the laboratory of Management Program of Northeast Dairy Herds (PROGEN), at Federal Rural University of Pernambuco (UFRPE) for analysis of milk composition and quality. The characterization of genotypes was performed by PCR-RFLP, for which were designed specific primers for each studied gene and restriction enzymes Kpn2I, HaeIII and HinfI that cut the DNA of the following genes: leptin, β-lactoglobulin and a PIT, respectively. The leptin estimate genotypic frequence were CC 0.112, TT 0.225 and CT 0.661, for β-lactoglobulin were AA 0.136, AB 0.323 and BB 0.539, and for PIT were ++ 0.655, -- 0.311 and +- 0.032. The results show that the population is in Hardy-Weinberg disequilibrium for leptin, β-lactoglobulin and a PIT due to excess of heterozygotes in the population, however, as these genes are associated with the milk production it is considered that the animals have genetic potential for milk production in the Brazilian semi-arid conditions. Through the characterization of the studied herd there were not found implications of the polymorphism of leptin, β-lactoglobulin and PIT in the composition and quality of milk from cows in the different genetic groups 1/2, 3/4 and 7/8 Holstein x Guzerat. Key words: β-lactoglobulin, crossbred cows, leptin, PCR-RFLP, PIT1, semi-arid.

Consumo e digestibilidade aparente de dietas com níveis crescentes do subproduto do caju em ovinos

The present study was conducted to evaluate the intake and digestibility of diets containing increasing levels of byproduct of cashew in sheep. The animals were distributed in a completely randomized design were evaluated in four levels (0%, 20%, 40%, 60%) by product of cashew with four replicates, making up 16 observations. The indicator used was the Purified Lignin and Enriched - LIPE ®. The scorer was orally administered directly into the mouth of the animals, in the form of capsules 250mg/animal/dia for a period of two days and five days of adaptation samples being the same supplied with the aid of a hose polyethylene and a device allowing the release of the capsule in the esophagus of sheep. With the estimate made by the indicator LIPE was observed a reduction for DM, OM, CP, NDF, EE, NFC and MM along the inclusion of byproduct of cashew. The results of nutrient digestibility were not satisfactory with the inclusion of byproduct of cashew, reducing linearly with the inclusion of the diets. The use of increasing levels of byproduct of cashew in the diets of sheep did not provide satisfactory results, it is not feasible to use the animals studied in this experiment

Sistema de produção misto de caprino na região central do estado do Rio Grande do Norte

This study aimed to evaluate the performance and profitability of Canindé goats kept under semi-intensive management in mix farming system. 84 animals were used, being 81 does e three bucks.. The means of fertility rate at parturition, prolificacy and percentages of pregnant, kid survival and reproductive rate were compared by Tukey test at significance level of 5.0%. The kid weights from birth to weaning were submitted to variance analysis considering sex and birth type and the means were compared by Tukey test at 5.0% of significance. The percentages of fertility rate by ultrassographic were 91.36 and parturition 74.84. The prolificacy was 1.53. Independently of sex the mortality rate of kids from birth to weaning was 12.3. The average weights of offspring were 2.11 and 1.92 kg and 6.74 and 6.84 kg for male and female offspring at birth and weaning, in this order. The average weights of kids, meaning PN, P28, P56, P63, in the first season of kidding were 2.11 ± 0.46 kg, 3.63 ± 1.04 kg, 5.96 ± 158 kg and 6.74 ± 1.79 kg. There was significant differences (P<0,05) only to birth weight of kids sons of buck 135. However, the fertility at parturition for first and second breeding seasons (BS) were 87.65 and 62.96, respectively (P<0.05). The prolificacy was 1.58 and 1.47 to first and second BS in this order. The percentages of offspring survival from birth to weaning, to first and second BS were 92.86 and 80.00, respectively. From the first BS the reproduction rate was 1.28 while in the second was 0.74. After weaning the duration of lactation was 104 days to first breeding parturition and 38 days in the second one. The total milk production was 853.50 kg. The kids weight gain was influenced by season of year, wet and dry and buck (P<0.05). Were concluded that the seasons of year when breeding season and parturition occur strongly affected the productive performance and profitability of production system

Parâmetros parasitológicos e desempenho produtivo de ovinos naturalmente infectados com nematoides gastrintestinais mantidos em diferentes pastagens tropicais

The control of gastrointestinal nematodes of small ruminants is usually done with anthelmintics. However, due to the emergence of ever-increasing parasite resistance to these drugs, looking up an alternative control parasites. One of this is sought in pasture management, as these are the sources of animals` infection by L3 infective larvae of helminths. The aim of this study was to evaluate the performance of sheep to natural infections by gastrointestinal nematodes grazing Panicum maximum cv. Massai and cv. Aruana, and Brachiaria brizantha cv. Piatã and cv. Marandu. The work was conducted from May to August-2011 with 48 male sheeps SRD versus Santa Inês breed. The animals were naturally infected with gastrointestinal nematodes, and maintained in four different cultivars of tropical forage grasses, naturally contaminated with eggs and larvae of gastrointestinal nematodes. Each week the animals were phenotypically characterized by parasitological examinations (eggs per gram of feces - EPG, and feces culture), hematological (packed cell volume PCV, and blood eosinophil count) method to evaluate the Famacha© colorof ocular mucosa, and the measures of body condition score and weight. In pastures was made the recovery of infective larvae in order to determine the quantity of L3 present in the pasture. The experimental design was a randomized completed block with two replications and before the entry of animals in the paddocks, they have been wormed. The experiment was ended when the animals reached 32.0 kg liveweight, and then were slaughtered and autopsies performed for the recovery and identify parasites of the abomasum, small intestine and large intestine. Results were subjected to analysis of variance, t test and Tukey`s test. The animals kept on pastures of Marandu grass had lower EPG counts, higher percentage of packed cell volume and higher average weight; those who remained in the Piatã pasture had lower eosinophil counts per microliter of blood. About the Famacha©, the highest prevalence was Famacha 2, and the body condition score ranged between 2 and 3. The results of feces cultures and recovery of larvae on pastures showed the presence of larvae of Trichostrongylus sp., and at the necropsy too. This way, it was concluded that the grass cultivars influences the sheep parasite load; the Famacha, together with EPG and packed cell volume are important indicators for use in controlling gastrointestinal nematode infections. The Trichostrongylus sp. was the most prevalent parasite in sheep during the rainy season

Estimação de parâmetros genéticos para resistência à infecção por IMNV em camarões Litopenaeus vannamei por meio de análise de sobrevivência

The main specie of marine shrimp raised at Brazil and in the world is Litopenaeus vannamei, which had arrived in Brazil in the `80s. However, the entry of infectious myonecrosis virus (IMNV), causing the infectious myonecrosis disease in marine shrimps, brought economic losses to the national shrimp farming, with up to 70% of mortality in the shrimp production. In this way, the objective was to evaluate the survival of shrimps Litopenaeus vannamei infected with IMNV using the non parametric estimator of Kaplan-Meier and a model of frailty for grouped data. It were conducted three tests of viral challenges lasting 20 days each, at different periods of the year, keeping the parameters of pH, temperature, oxygen and ammonia monitored daily. It was evaluated 60 full-sib families of L. vannamei infected by IMNV in each viral challenge. The confirmation of the infection by IMNV was performed using the technique of PCR in real time through Sybr Green dye. Using the Kaplan-Meier estimator it was possible to detect significant differences (p <0.0001) between the survival curves of families and tanks and also in the joint analysis between viral challenges. It were estimated in each challenge, genetic parameters such as genetic value of family, it`s respective rate risk (frailty), and heritability in the logarithmic scale through the frailty model for grouped data. The heritability estimates were respectively 0.59; 0.36; and 0.59 in the viral challenges 1; 2; and 3, and it was also possible to identify families that have lower and higher rates of risk for the disease. These results can be used for selecting families more resistant to the IMNV infection and to include characteristic of disease resistance in L. vannamei into the genetic improvement programs

Utilização de bagaço de caju desidratado e complexo enzimático na ração para codornas japonesas em postura

Given the significant share of food costs of poultry production, it is necessary to use strategies and techniques to maximize the utilization and biological value of the components of the diets, keeping constant or improving animal performance. In this context, seeking partial substitution of corn and the best use of the constituents of the diet, the present study aimed to evaluate the effects of inclusion of cashew pomace dehydrated (BCD) and enzyme complex (EC) in the diet on performance and quality eggs of Japanese quails. A total of 200 Japanese quail at 87 days of age, in 25 cages in a completely randomized design in a factorial 2 x 2 + 1 (two levels of cashew bagasse x two levels of enzymes) + control diet without cashew bagasse totaling five treatments with five replicates of eight birds each repetition. The experiment lasted 84 days, divided into four periods of 21 days. The treatments consisted of T1-Ration Control, T2-ration with 7.5% without EC BCD, T3-ration with 7.5% BCD with CE, T4-Ration with 15% BCD without EC and T5-Feed with 15% of BCD with EC. The enzyme complex (EC) is used fermentation product of Aspergillus niger, and cashew bagasse was obtained from the juice industry, passed through drying and crushing process for producing the bran. The performance and egg quality of quails, and performance variables: feed intake (FI), feed conversion ratio per dozen eggs (CAKDZ) and egg mass (CAMO), egg production (OP% bird / day), average egg weight (PMO) and egg mass (MO), and variables were egg quality, specific gravity (SG), Haugh unit (HU), yolk index, and the relationships between components eggs (%albumen,% yolk and %shell). Data were analyzed with the Statistical SAEG (2007). The 7.5 and 15% of cashew bagasse, independent of the enzyme complex decreased feed intake and improved the average egg weight. The two levels with the addition of EC showed significant differences for feed conversion by egg mass. The 7.5 with the addition of enzyme complex obtained the lowest average feed conversion per dozen eggs. The inclusion of up to 15% of cashew bagasse dehydrated with or without addition of enzyme complex in the diet of Japanese quails not interfere in the internal and external quality of eggs. The 7.5 and 15% BCD without added enzyme complex gave better means for specific gravity. Under conditions in which the experiment was conducted, it can be stated that the inclusion of up to 15% of cashew bagasse dehydrated with added enzyme complex feeding quail is economically viable, with the level of 7.5% with the addition of complex enzyme presented the best economic indices

Caracterização do sistema agropecuário dentro da agricultura familiar como subsídio de políticas públicas

The objective of this study was to characterize the socioeconomic profile of the family farmers who live and work in Brazilian rural space and highlight the importance of family agribusiness through the significant relation between some variables as the value of the total production, GDP share in total area of the establishments, agricultural production and farmer income, that show the important participation of these farmers in agricultural productivity and consequently on the economy of country. Therefore, this sector deserves a greater attention and more investment in the development of public policies that lead to a quality education in rural areas, availability of technical courses for farmers, technical assistance more efficient and effective, as well as funding more readily available

Avaliação genética da eficiência reprodutiva em vacas pardo-suíças por meio da análise de sobrevivência

Among the traits of economic importance to dairy cattle livestock those related to sexual precocity and longevity of the herd are essential to the success of the activity, because the stayability time of a cow in a herd is determined by their productive and reproductive lives. In Brazil, there are few studies about the reproductive efficiency of Swiss-Brown cows and no study was found using the methodology of survival analysis applied to this breed. Thus, in the first chapter of this study, the age at first calving from Swiss-Brown heifers was analyzed as the time until the event by the nonparametric method of Kaplan-Meier and the gamma shared frailty model, under the survival analysis methodology. Survival and hazard rate curves associated with this event were estimated and identified the influence of covariates on such time. The mean and median times at the first calving were 987.77 and 1,003 days, respectively, and significant covariates by the Log-Rank test, through Kaplan-Meier analysis, were birth season, calving year, sire (cow s father) and calving season. In the analysis by frailty model, the breeding values and the frailties of the sires (fathers) for the calving were predicted modeling the risk function of each cow as a function of the birth season as fixed covariate and sire as random covariate. The frailty followed the gamma distribution. Sires with high and positive breeding values possess high frailties, what means shorter survival time of their daughters to the event, i.e., reduction in the age at first calving of them. The second chapter aimed to evaluate the longevity of dairy cows using the nonparametric Kaplan-Meier and the Cox and Weibull proportional hazards models. It were simulated 10,000 records of the longevity trait from Brown-Swiss cows involving their respective times until the occurrence of five consecutive calvings (event), considered here as typical of a long-lived cow. The covariates considered in the database were age at first calving, herd and sire (cow s father). All covariates had influence on the longevity of cows by Log-Rank and Wilcoxon tests. The mean and median times to the occurrence of the event were 2,436.285 and 2,437 days, respectively. Sires that have higher breeding values also have a greater risk of that their daughters reach the five consecutive calvings until 84 months

Estrutura do dossel e acúmulo de forragem dos capins piatã e marandu sob pastejo com ovinos

The objective of this scientific work was to evaluate the tillering and mass production of Brachiaria brizantha Piatã and Marandu grasses with two statures of post-grazing. The experimental lineation was in factorial arrangement (2 pastures x 2 residue stature) with subplots (grazing cycle). The experimental period was from March to October of 2012. The target stature on pre-grazing was 50 cm and on post-grazing 15 and 25 for each cultivar. The following variables were analyzed: tiller population density, forage mass, morphological components, accumulation and accumulation rate. Three grazing cycles were obtained. Marandu grass presented larger forage mass on pre-grazing and lower stem production than piatã grass. No difference was observed between the two cultivars regarding the leaf blade production. There was no difference on forage mass, leaf blade, stem, and dead material production between the two statures of post-grazing. The pastures managed at 25 cm presented accumulation rate compared to those with 15 cm. Concerning the accumulation rate on the two cultivars, piatã and marandu grasses, Marandu grass presented higher amount than the piatã grass, and also presented higher values on the rate of basal tiller appearing on 15 cm stature post-pasture feature. Piatã grass managed at 25 cm developed smaller tiller amount on the second generation and greater tiller amount on the third generation than marandu grass. The greater tiller population density was observed on 25cm post-grazing pastures. There was no statistical difference of density between the two cultivars and between the cycles. Accordingly, it is concluded that piatã and marandu cultivars can be managed with 15cm and 25 cm post-grazing statures

Desempenho de novilhos alimentados com dieta suplementar em pastos de capim-mombaça

The objective of this work was to evaluate the effect of supplements feeding on growth of calves grazing a Panicum maximum cv. Mombaça pasture during the dry season. The experimental design was a randomized blocks with three treatments and three replications. The treatments were: mineral salt ad libidum; multiple mixture (MM) fed at 0.2% of live weight (PV); and, concentrate feed (SC) fed at 0.7% of PV. Thirty six weaned calves averaging eight months and 192 kg of initial live weight were utilized. The masses and pasture components, nutritive value and rate of forage growth were evaluated. Animal performance was measured as average daily gain (ADG) and live weight gain (LWG). The supplemental feeding was adjusted after weighing. There was no difference between periods for forage mass and leaf: stem ratio. The highest values for forage green mass, leaf blades mass and stem percentage were observed in the first trial period. The canopy height and the available forage on offer did not differ among treatments. The percentage of dead was higher for the last periods of evaluation. The leaf: stem ratio and the leaf percentage were greater in the second period. There was significant difference (p<0,05) among treatments for the ADG and were 250, 460 and 770 g/day for salt, MM and SC, respectively. The biggest LWG was observed in the treatment SC. contents of PB, DIVMO, NDF and LDA on leaf blades, thatched roofs and dead material dead not differ among treatments. The highest GPV was observed in the SC treatment. The contents of PB, DIVMO, NDF and LDA for leaf blades stem and dead material did not differ among treatments. Independent of the use supplements , it is possible to keep steers gaining weight, during dry season, since the stocking rate is appropriately adjusted

Determinação da composição química de quatro variedades de feijão caupi (Vigna unguiculata L. Walp) cultivadas em solo potiguar

The cowpea (Vigna unguiculata L. Walp) is a major food crops in northeastern Brazil. In Rio Grande do Norte, the cowpea, vigna beans or cowpea, as it is known, has great socioeconomic importance as a source of nutrients in food, with great emphasis among agricultural products. To improve productivity and resistance to pests, two cultivars were developed exclusively by EMPARN (Agricultural Research Corporation of Rio Grande do Norte), for breeding. The samples were provided by EMPARN, two improved (Potiguar and Laugh year) and two landraces (Rib of beef and Canapu). The seeds were ground and made into flour samples and the determination of moisture and ash by graviméticos methods, lipids by Soxhlet extraction, fibers with determiner fiber, carbohydrates by difference and minerals by ICP-OES were performed except the match analyzed by UVvis. The results showed a high fiber content (55.55% and 55.32% and 50.04% improved samples and 50.32% creole samples) and protein (25.52% and 25.27% improved and 27 samples, 23% and 24.99% creole samples). Comparing the results of the mineral content, the improved cultivars stood out in relation to Ca, Co, P, Mg, Mo and Na. Creole cultivars showed better results for Cu, Cr (low), Fe, Mn, Ni, K and Zn. The results will be important in future nutritional research and to build a table of Brazilian chemical composition of foods.

Estratégias para a organização da pesquisa em cana-de-açúcar: uma análise de governança em sistemas de inovação

PEDRO, Edilson da Silva. Estratégias para a organização da pesquisa em cana-de-açúcar: uma análise de governança em sistemas de inovação. 2008. 226f. Tese (Doutorado em Política Científica e Tecnológica) - Universidade Estadual de Campinas, Campinas, 2008.