Análise citogenética de células-tronco derivadas do subendotélio da veia umbilical humana

Mesenchymal stem cells (MSCs) are known as a population of multi-potential cells able to proliferate and differentiate into multiple mesodermal tissues including bone, cartilage, muscle, ligament, tendon, fat and stroma. Several applications of the study of EC can be emphasized the therapeutic techniques such as guided bone regeneration by implantation of EC in the affected site, without the need for bone grafts, using titanium as a vehicle. The process of cryopreservation is essential for the maintenance of cell cultures, since the cell line is frozen, it can be maintained in liquid nitrogen for an indefinite period and then thawed for therapeutic or experimental purposes. The aim of this study was to isolate a population of MSCs derived from the subendothelium of the umbilical vein human (MSCs-SUVH) to assess cytogenetic analysis by the possibility of appearance of chromosomal changes in two different situations: MSCs-SUVH regarding the process of cryopreservation and MSCs-SUVH grown on the surface of titanium. Flow cytometry analysis revealed that, this cell population was positive for the markers CD29, CD73 and CD90, but there was no expression of hematopoietic lineage markers, such as CD14, CD34 and CD45 and demonstrated capacity for osteogenic differentiation. The chromosomes obtained from the primary culture of MSCs-SUVH were analyzed by GTW banding technique, and results are described as guidelines to ISCN 2005. There was not the emergence of clonal chromosomal changes in the MSCs-SUVH in different situations analyzed. However one of the strings presented a balanced paracentric inversion, probably a cytogenetic constitutional alterations, which was present before and after the experimental situations that the MSCs-SUVH was submitted

Tessituras poéticas da corporeidade na formação humana do educador infantil

Cette étude a comme sujet principal la formation humaine de l instituteur. L objectif général est d analyser et décrire les conditions créées par les vécus d un atelier corps biographique pour la resignification de la corporéité de l instituteur, fixant les questions suivantes pour l investigation : Quelles sont les implications du vécu de l atelier corps biographique dans la vie des instituteurs ? La recherche a été exposée sur l approche qualitative, suivant les principes de la recherche-action reliée à la méthode des histoires de vie. Pour révéler la beauté et la complexité du processus poïétique de la formation humaine, nous nous sommes servi de la métaphore du "dessus de lit en patchwork" comme opérateur cognitif en attachant les principes de la corporéité fondés sur la Théorie de la complexité (MORIN, 2005), la Théorie autopoïétique (MATURANA ; VARELA, 2001), la Théorie du flux (CSIKSZENTMIHALYI, 1999), les approches de transdisciplinarité (LA TORRE; MORAES, 2008), et d autobiographie (JOSSO, 2004; PINEAU, 2003; PASSEGGI, 2000;), joignant la conception de l éducation comme pratique d autonomie, de liberté, d espoir et de l enchantement (FREIRE, 1996, 1992; ASSMANN, 1995). Nous avons eu comme scène du développement de la recherche, l Unidade Educacional Infantil (UEI), la garderie qui accueille les enfants des fonctionnaires de l Université Fédérale du Rio Grande do Norte. Treise élèves (enfants), des institutrices titulaires, vacataires, boursières ont participé de l'investigation. En conptant sur eux, nous avons développé un atelier corps biographique, dans lequel nous avons réalisé 10 vécus dans la période qui a couvert le deuxième semestre de 2007 jusqu au deuxième semestre de 2008, en manifestant la réflexivité autobiographique, le ludique, la créativité, la sensibilité et la réflexivité du vécu. Nous avons utilisé comme instruments pour la construction des donnés, l observation participante, les propres vécus, le port folio, la technique du jeu de sable et l'enregistrement photographique. L analyse a montré que le vécu de la formation humaine à partir des principes de la corporéité, implique dans un parcours vers soi-même dans lequel émergent les expériences qui nous ont constitués en tant qu êtres existentiels. Pour finaliser, nous avons aperçu les répercussions dans la vie professionnelle et personnelle de ces institutrices, qui comme moi pensaient avoir besoin d exposer leur luminosité intérieure pour que l on la redécouvre. Lors des vécus des tissages, les institutrices se sont montrées impliquées aux moments de jeux, de création, de réalisation, d afection et d action réflexive, dans lesquelles la teneur humaine cachée, a été révélée, en ouvrant les possibilités pour le sentiment du mouvement de naissance de l humanisation intérieure. Pour conclure, le corps joyeux de savoir et de beauté a été révélé, et dans sa rencontre avec soi-même et avec l autre, a pu être auto- recréé

Ecologia humana e manejo participativo da pesca do búzio Anomalocardia brasiliana (Gmelin, 1791) (Bivalvia: Veneridae) na Reserva de Desenvolvimento Sustentável Estadual Ponta do Tubarão (RN)

Coastal and marine protected areas are created to protect habitat, avoid biodiversity loss, and to help maintain viable fisheries. However, most of these areas in tropical countries occurs in impoverished regions and directly affect the livelihood and survival of coastal communities which directly depend on fisheries and shellfisheries. Therefore, socioeconomic and conservation goals overlap. In this context, fishers should have a central place in resource management. They are critical resource users and their behavior directly affects the system. Shellfish resources are important sources of food, employment and income to fishing communities in Latin America. But despite its widespread use for food and income, there is an urgent need of more research on shellfish management. This research discusses the artisanal fisheries of Venus clam (Anomalocardia brasiliana) (Gmelin, 1791) (Bivalvia: Veneridae) in Brazil, and points out strategies to improve the system. Venus clam is a small and commonly exploited species for food and income on the Brazilian coast. This research was carried out at Ponta do Tubarão Sustainable Development Reserve (Brazilian Northeast coast), where there was no information available about who harvest, where or how much Venus clam has been harvested, despite this resource being exploited for generations. Clam fishery follows the pattern of socio-economic invisibility that general clam exploitation has in Brazil. Methods used were interviews, participatory monitoring and focal follow observation from January 2010 to May 2011. Results include: (a) the identification of shell fishers, (b) how harvest and meat processing are performed (mollusk beds, time spent, gross and net production), (c) the analisis of shell fisher income and their economic sustentability, and (d) the involvement of shell fisher families in data gathering and analyses for the first time. Based on the acquired knowledge, we propose a new institutional arrangement for clam fishery including co-management, fisheries agreement, compensatory arrangements and improvements for the Venus clam value chain such as the establishment of a minimum price for clam meat. This research also includes two other results: a general description for Venus clam harvesting in the Brazilian Northeast coast and a specific discussion about co-management of Venus clam in Brazil. The first one was possible through the meeting of several shell fisherwomen from other states during activities promoted by People of the Tides (PoT) project. PoT was an international initiative aiming to develop coastal communities that depend on mollusk for their livelihood. The second one is a comparison between PoT and Venus clam management at Pirajubaé Marine Extractive Reserve (Santa Catarina). It evaluates the success and failures of these only two initiatives involving co-management of A. brasiliana in Brazil