Análise de grafos aplicada a relatos de sonhos: ferramenta diagnóstica objetiva e diferencial para psicose esquizofrênica e bipolar

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

A influência da criopreservação nas células mesenquimais indiferenciadas do ligamento periodontal de humanos análise comparativa in vitro

Cryopreservation is a process where cells or biological tissues are preserved by freezing at very low temperatures and aims to cease reversibly, in a controlled manner, all the biological functions of living tissues, i.e., maintain cell preservation so that it can recover with high degree of viability and functional integrity. This study aimed to evaluate the influence of cryopreservation on the mesenchymal stem cells originating from the periodontal ligament of human third molars by in vitro experiments. Six healthy teeth were removed and the periodontal cells grown in culture medium containing α-MEM supplemented with antibiotics and 15% FBS in a humidified atmosphere with 5% CO2 at 37° C. Cells isolated from each sample were divided into two groups: Group I - immediate cell culture (not fresh cryopreserved cells) and Group II - cell cryopreservation, during a period of 30 days. Analyses of rates of cell adhesion and proliferation in different groups were performed by counting the cells adhered to the wells, in intervals of 24, 48 and 72 hours after the start of cultivation. The number of cells in each well was obtained by counting viable cells with the use of hemocytometer and the method of exclusion of cells stained by trypan blue. The difference between groups for each of the times was analyzed by Wilcoxon test. Regarding the temporal evolution for each group, analysis was done by Friedman's test to verify the existence of differences between times and, when it existed, the Wilcoxon penalty was applied. The results showed no statistically significant difference between the two groups analyzed in this study. Therefore, we conclude that the cryopreservation process, after a period of 30 days, did not influence the cell type studied, and there was no difference in growth capacity in vitro between the groups

Análise citoarquitetônica dos componentes do sistema de temporização circadiana do sagui (Calithrix jacchus), comparada com a inervação das suas principais aferências

The suprachiasmatic nucleus (SCN) of the anterior hypothalamus, together with the intergeniculate leaflet (IGL) of the thalamus are considered the central components of the circadian timing system (CTS) of mammals. This system is responsible for the generation and regulation of circadian rhythms by establishing a temporal organization of physiological processes and behaviors. The neuronal specific nuclear protein (NeuN) has been widely used as a neuronal marker in several studies. Since glial fibrillary acidic protein (GFAP) is a component of intermediate filaments found in the cytoplasm of astrocytes and is commonly used as a specific marker for these cells. This study aims to identify, in the marmoset, the NeuN immunoreactive neurons and glial cells immunoreactive to GFAP, as well as map the major route of photic synchronization of the STC, retinohypothalamic tract (RHT), and identify the indirect pathway to the SCN and pregeniculate nucleus (PGN) - structure homologous to IGL rodents, using immunohistochemical and cytoarchitectonic techniques. Observed in SCN the presence of neurons immunoreactive to NeuN and terminals immunoreactive subunit b of cholera toxin (CTb), neuropeptide Y (NPY) and serotonin (5- HT). In the PGN noted the presence of the NeuN and NPY immunoreactive neurons and the immunoreactive terminals CTb and 5-HT. Astrocytes are present throughout the extent of the SCN and the PGN this New World primate

Variação temporal e descrição de novas espécies de collembola (arthropoda, hexapoda) em uma área de caatinga do nordeste do Brasil

Collembola is one of the most abundant and diverse group of terrestrial arthropods, being at the base of the food chain operating in the decomposition process. They have a wide distribution in the world and can be found in practically all habitats. The knowledge of this distinctive fauna is still deficient in brazilian territory, especially in semi-arid region. The aim of this study was to investigate which climatic variables may act as predictors of species richness, abundance of individuals and compositional structure of the taxocenose of Collembola over 12 months in an area dominated by semi-arid Caatinga vegetation, northeastern Brazil and describe new species of the genus Seira found, more diverse taxon of Collembola in Brazil. Samples were collected in João Câmara, Rio Grande do Norte. Ten plots of 20 x 20 meters were established and the specimens were collected with collection effort of one hour/people using entomological aspirator. The identification and description of the species was carried out by studying the morphology and chaetotaxy. Was performed a multiple regression analysis between species richness and abundance of individuals with climatic variables. A total of 1231 individuals belonging to 15 species, 12 genera and nine families. The greatest richness and abundance of Collembola were found during the rainy season. The genus Seira was the most abundant. Rainfall explained the temporal variation in species richness and abundance of Collembola in the semi-arid region, which is consistent with the biology of these animals. The populations of Collembola showed grouped distribution. Three new species of Seira were described and illustrated and all show similarities with species already registered in the national territory

Variação temporal e descrição de novas espécies de collembola (arthropoda, hexapoda) em uma área de caatinga do nordeste do Brasil

Collembola is one of the most abundant and diverse group of terrestrial arthropods, being at the base of the food chain operating in the decomposition process. They have a wide distribution in the world and can be found in practically all habitats. The knowledge of this distinctive fauna is still deficient in brazilian territory, especially in semi-arid region. The aim of this study was to investigate which climatic variables may act as predictors of species richness, abundance of individuals and compositional structure of the taxocenose of Collembola over 12 months in an area dominated by semi-arid Caatinga vegetation, northeastern Brazil and describe new species of the genus Seira found, more diverse taxon of Collembola in Brazil. Samples were collected in João Câmara, Rio Grande do Norte. Ten plots of 20 x 20 meters were established and the specimens were collected with collection effort of one hour/people using entomological aspirator. The identification and description of the species was carried out by studying the morphology and chaetotaxy. Was performed a multiple regression analysis between species richness and abundance of individuals with climatic variables. A total of 1231 individuals belonging to 15 species, 12 genera and nine families. The greatest richness and abundance of Collembola were found during the rainy season. The genus Seira was the most abundant. Rainfall explained the temporal variation in species richness and abundance of Collembola in the semi-arid region, which is consistent with the biology of these animals. The populations of Collembola showed grouped distribution. Three new species of Seira were described and illustrated and all show similarities with species already registered in the national territory

Análise comparativa da tendência na mortalidade infantil em áreas cobertas e não cobertas pela estratégia saúde da família no município de Garanhuns entre 2003 e 2012

A mortalidade infantil é tida como um indicador sensível para descrever as condições de vida e de saúde de uma população, sendo, portanto, interpretada como a estimativa do risco de um nascido vivo morrer antes de completar o primeiro ano de vida. Esse indicador é considerado elevado quando atinge patamares superiores a 50/1.000 nascidos vivos, médios quando se encontra entre 20 e 49/1.000 e mais baixos quando está até 20/1.000. No Brasil, a Mortalidade Infantil tem evidenciado variações ao longo dos anos, e nas duas últimas décadas esse indicador tem sofrido um acentuado decréscimo, provavelmente devido à melhoria no acesso aos serviços de saúde, ao saneamento básico, redução da taxa de fecundidade, melhoria das condições de vida e implementação de tecnologias na atenção à saúde. O objetivo principal do estudo foi avaliar a tendência na mortalidade infantil no município de Garanhuns no período de 2003 a 2012, segundo áreas cobertas e não cobertas pela estratégia saúde da família. Foi realizado um estudo de série temporal, e para isso foram coletados os dados referentes aos nascidos vivos e óbitos de menores de 01 (um) ano através do Sistema de Informações de Atenção Básica – SIAB, nas áreas cobertas e não cobertas pela estratégia, a fim de estabelecer relação de possível causalidade entre a intervenção e o indicador. Os resultados foram apresentados em gráficos, com a curva da Mortalidade Infantil no município de Garanhuns entre os anos de 2003 e 2012 segmentado através das áreas cobertas e não cobertas pela estratégia saúde da família ao longo do mesmo período. Após a análise dos resultados, observou-se uma tendência de queda no coeficiente de mortalidade infantil tanto nas áreas cobertas pela estratégia saúde da família quanto nas áreas cobertas pelo PACS, e que não foi possível estabelecer isoladamente uma maior redução da mortalidade infantil em áreas cobertas pela estratégia. No entanto, os resultados das ações desenvolvidas pela estratégia saúde da família são consistentes e plausíveis de causar impacto no declínio da mortalidade infantil, sobretudo as ações voltadas para a saúde da mulher e da criança

Análise wavelet e modelo de manchas em curvas de luz estelares dos telescópios espaciais Kepler e CoRoT

Analogous to sunspots and solar photospheric faculae, which visibility is modulated by stellar rotation, stellar active regions consist of cool spots and bright faculae caused by the magnetic field of the star. Such starspots are now well established as major tracers used to estimate the stellar rotation period, but their dynamic behavior may also be used to analyze other relevant phenomena such as the presence of magnetic activity and its cycles. To calculate the stellar rotation period, identify the presence of active regions and investigate if the star exhibits or not differential rotation, we apply two methods: a wavelet analysis and a spot model. The wavelet procedure is also applied here to study pulsation in order to identify specific signatures of this particular stellar variability for different types of pulsating variable stars. The wavelet transform has been used as a powerful tool for treating several problems in astrophysics. In this work, we show that the time-frequency analysis of stellar light curves using the wavelet transform is a practical tool for identifying rotation, magnetic activity, and pulsation signatures. We present the wavelet spectral composition and multiscale variations of the time series for four classes of stars: targets dominated by magnetic activity, stars with transiting planets, those with binary transits, and pulsating stars. We applied the Morlet wavelet (6th order), which offers high time and frequency resolution. By applying the wavelet transform to the signal, we obtain the wavelet local and global power spectra. The first is interpreted as energy distribution of the signal in time-frequency space, and the second is obtained by time integration of the local map. Since the wavelet transform is a useful mathematical tool for nonstationary signals, this technique applied to Kepler and CoRoT light curves allows us to clearly identify particular signatures for different phenomena. In particular, patterns were identified for the temporal evolution of the rotation period and other periodicity due to active regions affecting these light curves. In addition, a beat-pattern vii signature in the local wavelet map of pulsating stars over the entire time span was also detected. The second method is based on starspots detection during transits of an extrasolar planet orbiting its host star. As a planet eclipses its parent star, we can detect physical phenomena on the surface of the star. If a dark spot on the disk of the star is partially or totally eclipsed, the integrated stellar luminosity will increase slightly. By analyzing the transit light curve it is possible to infer the physical properties of starspots, such as size, intensity, position and temperature. By detecting the same spot on consecutive transits, it is possible to obtain additional information such as the stellar rotation period in the planetary transit latitude, differential rotation, and magnetic activity cycles. Transit observations of CoRoT-18 and Kepler-17 were used to implement this model.

Análise textual-interativa das Cartas dos Sertões do Seridó: em busca de efeitos estético-estilísticos

This work takes as its object of study a specific set of texts, designated letters about Sertões do Seridó (interstate region in the Brazilian northeastern), written by Paulo Bezerra, whose main approach is the backcountry universe. For its unique character, mainly because of a strong poetic appeal, we infer that these letters can also contain natural phenomena related to discourse-compositional structure, which may be responsible for creating specific effects, particularly aesthetical. In order to discuss and develop the issue, we set the theoretical assumptions of Textual-Interactive Perspective whose base is the concept of language as interaction, such as verbal activity impregnated with the space-time social and historical context in which the interlocutors are related, we chose the analytical category discursive topic and analyse introduction, sequencing and change of topic mechanisms. The goal is to determine the functionality of these mechanisms, noting how correlate the structural and interactional aspects are, and how this movement can be used to explain some of the aesthetic and stylistic effects of these letters. The result shows that it is used different topic organizational patterns according to the nature of the central topic. These different mechanisms express an aesthetic intention and feature a style. This result gives rise to more general conclusions about the texts: their discursive-compositional structure is related to the creation of aesthetics and stylistics effects.

Análise de tendência e caracterização sazonal e interanual da evapotranspiração de referência para o sudoeste da amazônia brasileira: Acre, Brasil

The objective of this research was to investigate monthly climatological, seasonal, annual and interdecadal of the reference evapotranspiration (ETo) in Acre state in order to better understand its spatial and temporal variability and identify possible trends in the region. The study was conducted with data from Rio Branco municipalities, the state capital, Tarauacá and Cruzeiro do Sul considering a 30-year period (1985-2014), from monthly data from weather stations surface of the National Institute of Meteorology. The methodology was held, first, the consistency of meteorological data. Thus, it was made the gap filling in the time series by means of multivariate techniques. Subsequently were performed statistical tests trend (Mann-Kendall) and homogeneity, by Sen's estimator of the magnitude of this trend is estimated, as well as computational algorithms containing parametric and non-parametric tests for two samples to identify from that year the trend has become significant. Finally, analysis of variance technique (ANOVA) was adopted in order to verify whether there were significant differences in average annual evapotranspiration between locations. The indirect method of Penman-Montheith parameterized by FAO was used to calculate the ETo. The results of this work through examination of the descriptive statistics showed that the ETo the annual average was 3.80, 2.92 and 2.86 mm day-1 year, to Rio Branco, Tarauacá and Cruzeiro do Sul, respectively. Featuring quite remarkable seasonal pattern with a minimum in June and a maximum in October, with Rio Branco to town one with the strongest signal (amplitudes) on the other hand, the Southern Cross presented the highest variability among the studied locations. By ANOVA it was found that the average annual statistically different for a significance level of 1% between locations, but the annual average between Cruzeiro do Sul and Tarauacá no statistically significant differences. For the three locations, the 2000s was the one with the highest ETo values associated with warmer waters of the North Atlantic basin and the 80s to lower values, associated with cooler waters of this basin. By analyzing the Mann-kendall and Sen estimator test, there was a trend of increasing the seasonal reference evapotranspiration (fall, winter and spring) on the order of 0.11 mm per decade and that from the years of 1990, 1996 and 2001 became statistically significant to the localities of Cruzeiro do Sul Tarauacá and Rio Branco, respectively. For trend analysis of meteorological parameters was observed positive trend in the 5% level of significance, for average temperature, minimum temperature and solar radiation.

Análise de métodos para detecção de mudanças estruturais em séries temporais climáticas

The time series analysis has played an increasingly important role in weather and climate studies. The success of these studies depends crucially on the knowledge of the quality of climate data such as, for instance, air temperature and rainfall data. For this reason, one of the main challenges for the researchers in this field is to obtain homogeneous series. A time series of climate data is considered homogeneous when the values of the observed data can change only due to climatic factors, i.e., without any interference from external non-climatic factors. Such non-climatic factors may produce undesirable effects in the time series, as unrealistic homogeneity breaks, trends and jumps. In the present work it was investigated climatic time series for the city of Natal, RN, namely air temperature and rainfall time series, for the period spanning from 1961 to 2012. The main purpose was to carry out an analysis in order to check the occurrence of homogeneity breaks or trends in the series under investigation. To this purpose, it was applied some basic statistical procedures, such as normality and independence tests. The occurrence of trends was investigated by linear regression analysis, as well as by the Spearman and Mann-Kendall tests. The homogeneity was investigated by the SNHT, as well as by the Easterling-Peterson and Mann-Whitney-Pettit tests. Analyzes with respect to normality showed divergence in their results. The von Neumann ratio test showed that in the case of the air temperature series the data are not independent and identically distributed (iid), whereas for the rainfall series the data are iid. According to the applied testings, both series display trends. The mean air temperature series displays an increasing trend, whereas the rainfall series shows an decreasing trend. Finally, the homogeneity tests revealed that all series under investigations present inhomogeneities, although they breaks depend on the applied test. In summary, the results showed that the chosen techniques may be applied in order to verify how well the studied time series are characterized. Therefore, these results should be used as a guide for further investigations about the statistical climatology of Natal or even of any other place.

Análise não-linear do campo de temperaturas desenvolvidas durante a soldagem do tipo TIG contínuo em placas delgadas

This study offers an analytical approach in order to provide a determination of the temperature field developed during the DC TIG welding of a thin plate of aluminum. The non-linear characteristics of the phenomenon, such as the dependence of the thermophysical and mechanical properties with temperature were considered in this study. In addition to the conductive heat exchange process, were taken into account the exchange by natural convection and radiation. A transient analysis is performed in order to obtain the temperature field as a function of time. It is also discussed a three-dimensional modeling of the heat source. The results obtained from the analytical model were be compared with the experimental ones and those available in the literature. The analytical results show a good correlation with the experimental ones available in the literature, thus proving the feasibility and efficiency of the analytical method for the simulation of the heat cycle for this welding process.

Estimativas, variabilidade e análise de tendência da evapotranspiração de referência em perímetros irrigados, no semiárido do nordeste brasileiro

The variability / climate change has generated great concern worldwide, is one of the major issues as global warming, which can is affecting the availability of water resources in irrigated perimeters. In the semiarid region of Northeastern Brazil it is known that there is a predominance of drought, but it is not enough known about trends in climate series of joint water loss by evaporation and transpiration (evapotranspiration). Therefore, this study aimed to analyze whether there is increase and / or decrease evidence in the regime of reference evapotranspiration (ETo), for the monthly, annual and interdecadal scales in irrigated polo towns of Juazeiro, BA (9 ° 24'S, 40 ° 26'W and 375,5m) and Petrolina, PE (09 ° 09'S, 40 ° 22'W and 376m), which is the main analysis objective. The daily meteorological data were provided by EMBRAPA Semiárido for the period from 01.01.1976 to 31.12.2014, estimated the daily ETo using the standard method of Penman-Monteith (EToPM) parameterized by Smith (1991). Other methods of more simplified estimatives were calculated and compared to EToPM, as the ones following: Solar Radiation (EToRS), Linacre (EToL), Hargreaves and Samani (EToHS) and the method of Class A pan (EToTCA). The main statistical analysis were non-parametric tests of homogeneity (Run), trend (Mann-kendall), magnitude of the trend (Sen) and early trend detection (Mann-Whitney). The statistical significance adopted was 5 and / or 1%. The Analysis of Variance - ANOVA was used to detect if there is a significant difference in mean interdecadal mean. For comparison between the methods of ETo, it were used the correlation test (r), the Student t test and Tukey levels of 5% significance. Finally, statistics Willmott et al. (1985) statistics was used to evaluate the concordance index and performance of simplified methods compared to the standard method. It obtained as main results that there was a decrease in the time series of EToPM in irrigated areas of Juazeiro, BA and Petrolina, PE, significant respectively at 1 and 5%, with an annual magnitude of -14.5 mm (Juazeiro) and -7.7 mm (Petrolina) and early trend in 1996. The methods which had better for better agreement with EToPM were EToRS with very good performance, in both locations, followed by the method of EToL with good performance (Juazeiro) and median (Petrolina). EToHS had the worst performance (bad) for both locations. It is suggested that this decrease of EToPM can be associated with the increase in irrigated agricultural areas and the construction of Sobradinho lake upstream of the perimeters.

Análise wavelet em curvas de luz estelares de sistemas binários da missão espacial CoRoT

Binary systems are key environments to study the fundamental properties of stars. In this work, we analyze 99 binary systems identified by the CoRoT space mission. From the study of the phase diagrams of these systems, our sample is divided into three groups: those whose systems are characterized by the variability relative to the binary eclipses; those presenting strong modulations probably due to the presence of stellar spots on the surface of star; and those whose systems have variability associated with the expansion and contraction of the surface layers. For eclipsing binary stars, phase diagrams are used to estimate the classification in regard to their morphology, based on the study of equipotential surfaces. In this context, to determine the rotation period, and to identify the presence of active regions, and to investigate if the star exhibits or not differential rotation and study stellar pulsation, we apply the wavelet procedure. The wavelet transform has been used as a powerful tool in the treatment of a large number of problems in astrophysics. Through the wavelet transform, one can perform an analysis in time-frequency light curves rich in details that contribute significantly to the study of phenomena associated with the rotation, the magnetic activity and stellar pulsations. In this work, we apply Morlet wavelet (6th order), which offers high time and frequency resolution and obtain local (energy distribution of the signal) and global (time integration of local map) wavelet power spectra. Using the wavelet analysis, we identify thirteen systems with periodicities related to the rotational modulation, besides the beating pattern signature in the local wavelet map of five pulsating stars over the entire time span.

Análise wavelet em curvas de luz estelares de sistemas binários da missão espacial CoRoT

Binary systems are key environments to study the fundamental properties of stars. In this work, we analyze 99 binary systems identified by the CoRoT space mission. From the study of the phase diagrams of these systems, our sample is divided into three groups: those whose systems are characterized by the variability relative to the binary eclipses; those presenting strong modulations probably due to the presence of stellar spots on the surface of star; and those whose systems have variability associated with the expansion and contraction of the surface layers. For eclipsing binary stars, phase diagrams are used to estimate the classification in regard to their morphology, based on the study of equipotential surfaces. In this context, to determine the rotation period, and to identify the presence of active regions, and to investigate if the star exhibits or not differential rotation and study stellar pulsation, we apply the wavelet procedure. The wavelet transform has been used as a powerful tool in the treatment of a large number of problems in astrophysics. Through the wavelet transform, one can perform an analysis in time-frequency light curves rich in details that contribute significantly to the study of phenomena associated with the rotation, the magnetic activity and stellar pulsations. In this work, we apply Morlet wavelet (6th order), which offers high time and frequency resolution and obtain local (energy distribution of the signal) and global (time integration of local map) wavelet power spectra. Using the wavelet analysis, we identify thirteen systems with periodicities related to the rotational modulation, besides the beating pattern signature in the local wavelet map of five pulsating stars over the entire time span.

Análise da concepção arquitetural à luz da arquiteturologia: um estudo da produção de edifícios de uso não-residencial do arquiteto João Maurício Fernandes de Miranda, entre 1961 e 1981

This study explores the architectural conception process using the Architecturologie as an instrument of analysis, based on Enseigner la Conception Architecturale (2000), developed by Boudon - architect, lecturer and French researcher. It begins with the selection of local architect´s works, João Maurício Fernandes de Miranda, which resulted in six non residential projects developed between 1961 and 1981. Architectural readings were developed, with emphasis in the identification of architecturological scales, their functions, relationship and modality of occurrence