A Pollen Chase Experiment; Examining Varying Levels of Embryonic Inbreeding Depression

A pollen chase experiment was performed upon three Costa Rican populations of Witheringia solanacea to examine the breakdown of genetically enforced self incompatibility (SI) and the extent of embryonic inbreeding depression. Self-pollen was applied in the bud, with outcross pollen applied one day later, and outcross pollinations at both intervals as a control. A variety of responses were found among the populations. BOHS readily accepted self pollen and suffered from very low inbreeding depression. Monteverde and Las Cruces both have lower fruit set with self-pollination precedence indicating that bud pollinations can overcome the self-incompatibility response and that embryonic death due to inbreeding depression causes fruit failure. The treatment:control fruit set is higher for the Las Cruces plants indicating stronger SI response Self-precedence seeds from the Las Cruces plants are likely to be outcrossed. Self-precedence seeds from Monteverde are likely selfed.

Corridors for Wolf Reintroduction to Maine

Maine has been identified as holding potential for the reintroduction of wolves. Due to the long ranges of wolves, it is beneficial to map potential corridors of movement. This project analyzes the best routes for movement from suitable habitats in New York to suitable habitats in Maine. It shows the paths likely to be taken by wolves, based on their affinity for mixed and coniferous forest and their avoidance of areas of high road density. These corridors are identified using least cost path analysis and take into account topography as well as forest and road densities.