1 resultado para DNA sequencing analysis
em Digital Archives@Colby
Filtro por publicador
- Acceda, el repositorio institucional de la Universidad de Las Palmas de Gran Canaria. España (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (6)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (1)
- Aquatic Commons (13)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (4)
- Archimer: Archive de l'Institut francais de recherche pour l'exploitation de la mer (2)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (2)
- Aston University Research Archive (5)
- B-Digital - Universidade Fernando Pessoa - Portugal (1)
- Biblioteca de Teses e Dissertações da USP (2)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (40)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (10)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (11)
- Bioline International (3)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (52)
- Brock University, Canada (7)
- Bucknell University Digital Commons - Pensilvania - USA (1)
- CaltechTHESIS (3)
- Cambridge University Engineering Department Publications Database (8)
- CentAUR: Central Archive University of Reading - UK (20)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (73)
- Cochin University of Science & Technology (CUSAT), India (2)
- CORA - Cork Open Research Archive - University College Cork - Ireland (8)
- Deakin Research Online - Australia (31)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (3)
- Digital Archives@Colby (1)
- Digital Commons - Michigan Tech (4)
- Digital Commons at Florida International University (6)
- DigitalCommons - The University of Maine Research (1)
- DigitalCommons@The Texas Medical Center (23)
- DigitalCommons@University of Nebraska - Lincoln (2)
- Duke University (12)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (24)
- FUNDAJ - Fundação Joaquim Nabuco (1)
- Helda - Digital Repository of University of Helsinki (8)
- Illinois Digital Environment for Access to Learning and Scholarship Repository (1)
- Indian Institute of Science - Bangalore - Índia (32)
- Infoteca EMBRAPA (1)
- Instituto Gulbenkian de Ciência (1)
- Instituto Politécnico do Porto, Portugal (4)
- Laboratório Nacional de Energia e Geologia - Portugal (1)
- Lume - Repositório Digital da Universidade Federal do Rio Grande do Sul (1)
- Massachusetts Institute of Technology (1)
- Memorial University Research Repository (2)
- National Center for Biotechnology Information - NCBI (52)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (6)
- Publishing Network for Geoscientific & Environmental Data (3)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (89)
- Queensland University of Technology - ePrints Archive (74)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (1)
- Repositório Científico da Universidade de Évora - Portugal (2)
- Repositório do Centro Hospitalar de Lisboa Central, EPE - Centro Hospitalar de Lisboa Central, EPE, Portugal (2)
- Repositório Institucional da Universidade de Aveiro - Portugal (3)
- Repositório Institucional da Universidade Estadual de São Paulo - UNESP (1)
- Repositório Institucional da Universidade Federal do Rio Grande do Norte (1)
- Repositorio Institucional de la Universidad de Málaga (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (185)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (1)
- School of Medicine, Washington University, United States (7)
- Universidad de Alicante (3)
- Universidad del Rosario, Colombia (5)
- Universidad Politécnica de Madrid (1)
- Universidade Federal do Pará (3)
- Universidade Federal do Rio Grande do Norte (UFRN) (2)
- Universita di Parma (2)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (3)
- Université de Lausanne, Switzerland (10)
- Université de Montréal (1)
- Université de Montréal, Canada (14)
- Université Laval Mémoires et thèses électroniques (1)
- University of Queensland eSpace - Australia (13)
- University of Southampton, United Kingdom (1)
- University of Washington (3)
- WestminsterResearch - UK (1)
Resumo:
Diepoxybutane (DEB), a known industrial carcinogen, reacts with DNA primarily at the N7 position of deoxyguanosine residues and creates interstrand cross-links at the sequence 5'-GNC. Since N7-N7 cross-links cause DNA to fragment upon heating, quantative polymerase chain reaction (QPCR) is being used in this experiment to measure the amount of DEB damage (lesion frequency) with three different targets-mitochondrial (unpackaged), open chromatin region, and closed chromatin region. Initial measurements of DEB damage within these three targets were not consistent because the template DNA was not the limiting reagent in the PCR. Follow-up PCR trials using a limiting amount of DNA are still in progress although initial experimentation looks promising. Sequencing of these three targets to confirm the primer targets has only been successfully performed for the closed chromatin target and does not match the sequence from NIH used to design that primer pair. Further sequencing trials need to be conducted on all three targets to assure that a mitochondrial, open chromatin, and closed chromatin region are actually being amplified in this experimental series.