Development of disease caused by Phytophthora cinnamomi in mature Xanthorrhoe australis

Over the past 30 years, heathland and open forest communities in south-eastern Australia dominated by Xanthorrhoea australis R.Br. have been severely affected by disease caused by Phytophthora cinnamomi Rands. The disease has caused a sharp decline in numbers of individuals within populations of X. australis; however, the etiology of the disease is unclear. The characteristics and disease symptoms induced by P. cinnamomi were analysed within nine mature X. australis plants that had been removed from the field. Seven plants showed typical disease symptoms that ranged from chlorotic leaves through to plant death. Plants showing disease symptoms had different numbers of infected roots, ranging from 0% in one dead plant, 40% infected roots in a plant showing yellowing of leaf tips and 67 and 86%, respectively, in two plants with severe chlorosis. There was variation within the roots, with some infected close to the stem while others were infected at more distal regions. Within stems of all plants, P. cinnamomi was difficult to isolate but was found in the desmium and stem apex and was associated with massive lesions within the central area of the stem. The symptoms of disease in X. australis are caused by a combination of damage to tissues of the roots and stem that may lead to a reduction in water and mineral transport throughout the plant.

Transcriptomics-based analysis using RNA-Seq of the coconut (Cocos nucifera) leaf in response to yellow decline phytoplasma infection

Invasive phytoplasmas wreak havoc on coconut palms worldwide, leading to high loss of income, food insecurity and extreme poverty of farmers in producing countries. Phytoplasmas as strictly biotrophic insect-transmitted bacterial pathogens instigate distinct changes in developmental processes and defence responses of the infected plants and manipulate plants to their own advantage; however, little is known about the cellular and molecular mechanisms underlying host–phytoplasma interactions. Further, phytoplasma-mediated transcriptional alterations in coconut palm genes have not yet been identified. This study evaluated the whole transcriptome profiles of naturally infected leaves of Cocos nucifera ecotype Malayan Red Dwarf in response to yellow decline phytoplasma from group 16SrXIV, using RNA-Seq technique. Transcriptomics-based analysis reported here identified genes involved in coconut innate immunity. The number of down-regulated genes in response to phytoplasma infection exceeded the number of genes up-regulated. Of the 39,873 differentially expressed unigenes, 21,860 unigenes were suppressed and 18,013 were induced following infection. Comparative analysis revealed that genes associated with defence signalling against biotic stimuli were significantly overexpressed in phytoplasma-infected leaves versus healthy coconut leaves. Genes involving cell rescue and defence, cellular transport, oxidative stress, hormone stimulus and metabolism, photosynthesis reduction, transcription and biosynthesis of secondary metabolites were differentially represented. Our transcriptome analysis unveiled a core set of genes associated with defence of coconut in response to phytoplasma attack, although several novel defence response candidate genes with unknown function have also been identified. This study constitutes valuable sequence resource for uncovering the resistance genes and/or susceptibility genes which can be used as genetic tools in disease resistance breeding.

Assessing the relationship between seagrass health and habitat quality with wasting disease prevalence in the Florida Keys

Marine pathogens of the genus Labyrinthula have been identified as the cause of wasting disease in seagrass systems in both temperate and subtropical regions. An understanding of the association between environmental factors and the prevalence of wasting disease in seagrass meadows is important for elucidating plant-pathogen interactions in coastal environments. We conducted a survey of 7 turtle grass-dominated beds within the Florida Keys National Marine Sanctuary to assess the relationship between environmental and biological parameters on seagrass health. All sites contained Labyrinthula spp.; the most pathogenic strain was obtained from an anthropogenically impacted site. Leaf and total biomass, in addition to root/rhizome carbon content, canopy light and % light transmitted, all displayed strong negative correlations with a wasting index (WI). It was noted that many of the same environmental measurements that showed negative correlations with WI also displayed strong positive correlations with canopy light levels. These data suggest that light availability may be an important factor that has previously been understated in the seagrass disease literature yet warrants more attention with respect to turtle grass susceptibility to infection. Studies such as this are important because they identify gaps in our understanding of plant-pathogen interactions in subtropical marine ecosystems. Furthermore, the relationships identified in this study may offer insight into which factors are most useful in identifying "at-risk" meadows prior to the onset of larger scale die-off events.