Two-photon endomicroscopy for 3D imaging of cancer cells labelled with gold nanorods

Biofunctional nanorods are developed to specifically target cancer cells. The cervical cancer cells, HeLa cells, are labeled by these biofunctional gold nanorods. Those cancer cells can be detected by a multi-photon-excited photoluminescence endomicroscope, which proves that the cancers can be in vivo diagnosed by using biofunctional gold nanorods with nonlinear endomicroscopy.

Two-photon imaging and photothermal therapy of cancer cells using biofunctional gold nanorods

Transferrin-conjugated gold nanorods were used for targeting, two-photon imaging and photothermal therapy of cancer cells. The presence of nanorods significantly reduced the laser power effective for therapy.

Photothermally induced apoptosis of cancer cells using gold nanorods for low energy cancer therapy

Gold nanorods functionalised with transferrin were used for photothermally induced necrosis and apoptosis of cancer cells. It was observed that the laser energy required to induce cell apoptosis is significantly lower than that for cell necrosis, indicating that photothermally induced apoptosis can be used for medically safe laser cancer treatment.

Enhanced photothermal therapy assisted with gold nanorods using a radially polarized beam

We report on the use of a radially polarized beam for photothermal therapy of cancer cells labeled with gold nanorods. Due to a three-dimensionally distributed electromagnetic field in the focal volume, the radially polarized beam is proven to be a highly efficient laser mode to excite gold nanorods randomly oriented in cancer cells. As a result, the energy fluence for effective cancer cell damage is reduced to one fifth of that required for a linearly polarized beam, which is only 9.3% of the medical safety level.

Gold-nanorod-facilitated nonlinear optical microscopy under radially polarised beam illumination

Reports on the use of radially polarised beam in gold-nanorod-facilitated nonlinear microscopy and therapy. It has been found that the use of radially polarised beam can greatly reduce the energy fluence threshold for treating cancer cells labelled with gold nanorods. The slight distortion in the polarisation properties of the radially polarised beam after propagating through double-clad photonic crystal fibres makes it promising in the application of fibre-optic based endoscopic system.

Cancer-cell microsurgery using nonlinear optical endomicroscopy

Near-infrared laser-based microsurgery is promising for noninvasive cancer treatment. To make it a safe technique, a therapeutic process should be controllable and energy efficient, which requires the cancer cells to be identifiable and observable. In this work, for the first time we use a miniaturized nonlinear optical endomicroscope to achieve microtreatment of cancer cells labeled with gold nanorods. Due to the high two-photon-excited photoluminescence of gold nanorods, HeLa cells inside a tissue phantom up to 250 μm deep can be imaged by the nonlinear optical endomicroscope. This facilitates microsurgery of selected cancer cells by inducing instant damage through the necrosis process, or by stopping cell proliferation through the apoptosis process. The results indicate that a combination of nonlinear endomicroscopy with gold nanoparticles is potentially viable for minimally invasive cancer treatment.

Functionalized mesoporous silica nanoparticles with redox-responsive short-chain gatekeepers for agrochemical delivery.

The controlled release of salicylic acid (SA), a key phytohormone, was mediated by using a novel decanethiol gatekeeper system grafted onto mesoporous silica nanoparticles (MSNs). The decanethiol was conjugated only to the external surfaces of the MSNs through glutathione (GSH)-cleavable disulfide linkages and the introduction of a process to assemble gatekeepers only on the outer surface so that the mesopore area can be maintained for high cargo loading. Raman and nitrogen sorption isotherm analyses confirmed the successful linkage of decanethiol to the surface of MSNs. The in vitro release of SA from decanethiol gated MSNs indicated that the release rate of SA in an environment with a certain amount of GSH was significantly higher than that without GSH. More importantly, in planta experiments showed the release of SA from decanethiol gated MSNs by GSH induced sustained expression of the plant defense gene PR-1 up to 7 days after introduction, while free SA caused an early peak in PR-1 expression which steadily decreased after 3 days. This study demonstrates the redox-responsive release of a phytohormone in vitro and also indicates the potential use of MSNs in planta as a controlled agrochemical delivery system.

Samurai in the surf : the arrival of the Japanese on the Gold Coast in the 1980s

Samurai in the Surf explores the history of Japanese investment in the 1980s, following a money-trail that leads back to a ballooning Japanese economy and a colorful array of investment sources. The book also explores the local community's views of this unprecedented stream of foreign investment and provides a convincing illustration of the links between 1980s Queensland and Pauline Hanson's One Nation movement of the 1990s.

Chemical and physical speciation of arsenic in a small pond receiving gold mine waste effluent

The chemical and physical speciation of arsenic in a small pond that receives wastewater from a gold mine operation in western Victoria, Australia was studied using differential pulse polarography. By using different sample pretreatments, distinction between the physical states (dissolved or particulate As), between the oxidation states (As(III) or As(V)), and between the degrees of lability (labile or strongly bound) was achieved. The results are interpreted in terms of the physicochemical properties with reference to the use of the pond as a settlement dam for gold mining effluent. The speciation of arsenic was found to vary markedly with the physicochemical properties of the water. A model for the behavior of arsenic in the pond is proposed.

How well does club head speed correlate with gold handicaps?

The velocity at which a golf club impacts with a golf ball is known as club head speed. Although club head speed has been used to measure performance changes in a number of golf studies, it has not been validated as a golf performance measure. As handicap is the usual measure of performance, the purpose of this study was to investigate the relationship between club head speed and handicap, and to determine whether club head speed at impact is a valid measure of golfing performance. Forty-five male golfers aged 18–80 years, all with registered golfing handicaps (2–27), participated in this study. Each golfer performed 10 golf swings captured by a high-speed camera. Golfers' club head speeds were determined using Video Expert 2, a biomechanical computer program. Golfers with a lower handicap (ie, a better skill level) had faster club head speeds than higher handicap golfers. Linear regression analysis found club head speed to be highly correlated with handicap (r= 0.950). This relationship was described by the equation: In (club head speed)= 4.065 − 0.0214 x handicap. In conclusion, this study has shown that club head speed is a valid indicator of performance in golfers and may therefore be a useful performance measure in future laboratory-based studies.

The online learning alchemist : preventing gold turning into lead

This paper addresses the creation of materials and resources for use in online learning, focusing on the new and emerging roles for teachers and learners in conjunction with developments in our understanding of the human-computer interface. As more educational providers adopt network-based technologies as delivery portals, the demand for skills in the creation of effective online resources is becoming critical. If we are to provide the learner with online resources that will enhance knowledge construction and the teacher with clear measures that these activities are effective, then we as resource developers must resurrect the role of what might be termed the online alchemist. Our first task is to ensure that new digital resources are not simply transferred from their original format but repurposed to ensure learner(s) accessing those resources are able to interact with both the content and their collaborative partners with new levels of flexibility and manipulation. We must transcend the too frequent use of technology as a means to replicate existing resources and conceptualise environments that engender new paradigms for teaching and learning. Our challenge remains to ensure the gold we have in effective teaching strategies and learning resources is not tarnished through ineffective applications within the online learning context. One strategy to achieve this is through proactive evaluation, a framework that integrates a set of factors and influences to better inform the development of online learning resources.

Mining more gold from web page hyperlinks

The development of the Internet has boosted prosperity of the World Wide Web, which is now a huge information source. Because of characteristics of the web, in most cases, traditional databasebased technologies are no longer suitable for web information retrieval and management. To effectively manage web information, it is necessary to reveal intrinsic relationships/structures among web information objects by eliminating noise factors. This paper proposes a mechanism that could be widely used in information processing, including web information processing and noise factor elimination for getting more intrinsic relationships. As an application case of this mechanism, one relevant web page finding algorithm is proposed to uncover intrinsic relationship among web pages from their hyperlink patterns, and find more semantic relevant web pages. The experimental evaluation shows the feasibility and effectiveness of the algorithm and demonstrates the potential of the proposed mechanism in web applications.