15 resultados para anaerobic biofilms

em Deakin Research Online - Australia


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Effluents from the juice and fruit processing industries have high organic matter content. Discharge of these effluents without appropriate treatment would therefore have a negative impact on the environment. High organic contents and low contamination levels make such effluents suitable for biological treatment, especially anaerobic digestion. In the latter process, significant amounts of digester gas can be produced, turning a waste stream into a source of renewable energy that can be used for electricity and heat production, leading to financial benefits.This paper investigates the feasibility of anaerobic digestion and the gas generation potential of five different effluents from the carrot-juice, orange-juice and sultana processing industries. Benefits are assessed in terms of digester gas production and organic matter reduction. The results show that the specific gas production ranges between 665 and 860 m3 per tonne of effluent treated (as organic dry matter). Furthermore, nearly 100% of the organic matter is converted into gas in the case of the carrot- and orange-juice processing residues, while a 84.5% reduction of the organic matter was found to be achievable in the case of the sultana wastes. While these results are promising, further testing will be required to validate them in a larger scale.

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In anaerobic degradation of substrates containing mainly particulate organic matter, solids hydrolysis is rate-limiting. In these investigations, the particle size of various substrates was reduced by comminution to support hydrolysis. Two positive effects of comminution were observed. For substrates with high fibre content, which are particularly resistant to biodegradation, a significant improvement of the degradation degree was observed as a result of comminution. Secondly, for all substrates tested, and particularly for those rich in fibres, the degradation rate of comminuted samples was significantly higher. The first reason for both effects is an increase of the sample surface area. Several methods for measuring the specific surface area of organic materials, including particle size analysis, Nitrogen-adsorption and enzyme adsorption, were used and compared for the purpose of this study, where the surface area accessible to microbial enzymes is critical. The significance of the surface area in anaerobic degradation of particulate substrates was investigated through a kinetic model where the hydrolysis rate was based on the sample surface area. Good agreements were obtained between model and experiments carried out with samples of various specific surface areas. These results reinforced the significance of the sample surface area in anaerobic degradation processes. However, other effects of comminution responsible for the increased degradation degree and degradation rate were identified and discussed. These include: the increase of dissolved compounds due to cell rupture, exposition of surface areas previously inaccessible for microbial degradation, and alteration of the sample structure such as the lignin-cellulose arrangements.

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The Victorian Environment Protection Authority (EPA) has identified Alcoa’s Point Henry aluminium smelter as being a major source of recognized pollutant input due to its disposal of effluent into Corio Bay. Historically, the water quality parameters that have most often exceeded Point Henry’s EPA limits have been pH and suspended solids from the smelter’s discharge points. These waste water discharges also experience high nitrogen and phosphorus concentrations which result in algal blooms that occur at the onset of warm weather. The main hypothesis of this study was that “prevention of algal blooming with the onset of warm weather by removal of nutrients during the cooler months, and continued removal thereafter, is better than curing the problems chemically”. Biofilms have been used to remove nutrients from waste waters, but not under the conditions experienced at Point Henry. The aim of this study, therefore, was to determine if significant biofilm growth would be observed on floating structures suspended in the Point Henry waste water stream during the cooler, winter months of the year. Statistically significant biofilm growth occurred on all suspended structures in all discharge ponds during the winter and early spring of 2000. The use of suspended structures, such as AquaMatTM, as an artificial substrate to attract and support periphyton and bacterial communities (biofilms), which are then able to out-compete phytoplankton communities for available nutrients, is therefore a viable option for the Point Henry smelter. However, further research on the competitive performance of biofilms in the Point Henry ponds during the summer months is required before adequate biofilm management strategies can be developed.

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Demonstrates that anaerobic capacity, using the accumulated oxygen deficit technique, can be measured rapidly and with a high degree of precision, however significant difficulties remain to be addressed. Significant further insights into some of the underlying cellular mechanisms associated with increases in anaerobic capacity were also obtained.

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Anaerobic fermentation experiments were conducted on banana (pseudo) stem residue to study the relationship between fermentation temperature and gas production yield and gas production rate, and methane content. Based on fixed dry matter concentration, inoculum concentration and fermentation time, different temperatures, i.e. 25, 30, 35, 40°C were selected and formed four experimental groups. Four levels of single factor tests were conducted to optimize temperature parameter for anaerobic fermentation of banana stem residue. The results showed that the daily gas yield of banana stem residue reached the maximum value of 36.8L on the fourth day at 35°C, and the average gas yield was 5.03L/d. The total gas yield was 402.3L, while the maximum methane content was 61.2% in the whole fermentation process. The results indicated that the comprehensive effect was best at 35°C in anaerobic fermentation of banana stem residue.

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A large number of team games require participants to repeatedly produce maximal or near maximal sprints of short duration with brief recovery periods. The purpose of the present study was to determine the relationship between a repeated sprint ability (RSA) test that is specific to the energy demands of Australian Rules football (ARF), and the aerobic and anaerobic energy systems. 


Seventeen ARF players participated in the study. Each participant was assessed for VO2 max, accumulated oxygen deficit (AOD), best 20 m sprint time and RSA. The RSA test involved 12x20 m sprints departing every 20 s. When including the work performed during the time taken to decelerate, the test involved a work to rest ratio of approximately 1:3. Total sprinting time and the percentage decrement of repeated sprinting times were the two derived measures of RSA.

The results indicate that the best 20 m sprint time was the only factor to correlate significantly with total sprinting time (r = 0.829, P<0.001) and percentage decrement (r = -0.722, P<0.01). VO2 max and AOD were not related to the total sprinting time or the percentage decrement that was produced by the RSA test. This was interpreted to signify that the phosphagen system was the major energy contributor for this test.