Diverse fibrillar peptides directly bind the Alzheimer’s amyloid precursor protein and amyloid precursor-like protein 2 resulting in cellular accumulation

The Alzheimer’s disease Aβ peptide can increase the levels of cell-associated amyloid precursor protein (APP) in vitro. To determine the specificity of this response for Aβ and whether it is related to cytotoxicity, we tested a diverse range of fibrillar peptides including amyloid-β (Aβ), the fibrillar prion peptides PrP106–126 and PrP178–193 and human islet-cell amylin. All these peptides increased the levels of APP and amyloid precursor-like protein 2 (APLP2) in primary cultures of astrocytes and neurons. Specificity was shown by a lack of change to amyloid precursor-like protein 1, τ-1 and cellular prion protein (PrPc) levels. APP and APLP2 levels were elevated only in cultures exposed to fibrillar peptides as assessed by electron microscopy and not in cultures treated with non-fibrillogenic peptide variants or aggregated lipoprotein. We found that PrP106–126 and the non-toxic but fibril-forming PrP178–193 increased APP levels in cultures derived from both wild-type and PrPc-deficient mice indicating that fibrillar peptides up-regulate APP through a non-cytotoxic mechanism and irrespective of parental protein expression. Fibrillar PrP106–126 and Aβ peptides bound recombinant APP and APLP2 suggesting the accumulation of these proteins was mediated by direct binding to the fibrillated peptide. This was supported by decreased APP accumulation following extensive washing of the cultures to remove fibrillar aggregates. Pre-incubation of fibrillar peptide with recombinant APP18–146, the putative fibril binding site, also abrogated the accumulation of APP. These findings show that diverse fibrillogenic peptides can induce accumulation of APP and APLP2 and this mechanism could contribute to pathogenesis in neurodegenerative disorders.

Australia's health 1994 : the fourth biennial report of the Australian Institute of Health and Welfare

Australia's Health is the most comprehensive and authoritative source of national information on health in Australia. Australia's Health is published mid-year in even-numbered years and provides national statistics and related information that form a record of health status, service provision and expenditure in Australia.

P F Thompson - Australia's pioneer in corrosion research and education

Percival Faraday Thompson (P F Thompson, PFT), (1885-1951),(Figure1), a metallurgistand chemical analyst, is widely considered as Australia’s pioneer in corrosion science and education. Each year at the annual ACA Conference, the P F Thompson Memorial Lectureis presented by a person who has made a most significant contribution to corrosion science and engineering in Australia. While each presenter usually gives a few detailsof the work of PFT for the benefit of Conference attendees, the entire body of his work and details about his life are not provided due to the limitations on the lecture duration. A review of Thompson’s life and some of his work is available in the ‘Australasian Corrosion Engineering’ publication which was the ACA publication in the early 70s, and is one of theprecursors to the current ACA publication Corrosion & Materials (Potter, 1972). However this publication is not widely available to the ACA membership, and certainly not inelectronic format. The purpose of this paper is to provide readers with some of the history of the PFT Lecture, and to acquaint readers of ‘Corrosion & Materials’ and the broader corrosion prevention community with the breadth of the scientific work of PFT and his enthusiasm for describing corrosion science through practical public demonstrations ofexperiments of the basic principles of corrosion.

Kerstin Thompson Architects : exploring affect in interior's sticky design process

Physical things influence emotions and feelings and can contribute to our sense that buildings and rooms are appropriately designed for their purposes. The sense of appropriateness extends, and is often noticed, through the pre-emotive effect of a structure’s shapes and dimensions. In interior discourse the relationship between the physical and pre-emotive is indicated with various terms (such as “affect”) and the design process of achieving appropriateness in objects and affect can be termed “stickiness”. In this paper we extend Sara Ahmed’s characterization of affect as a sticky connective element, which allows objects and ideas to generate attachments with us. And we ask, how can we understand interior practices and design processes through the concept of affect – as sticky? We explore this question first by discussing affect’s stickiness, and second, by an empirical study of the design process of Kerstin Thompson’s Monash University Museum of Art. The specific project involves alternating design methods that Thompson uses: an intuitive hunch-driven process, and a more defined literary-driven process. Our interest is to consider how she shifts from one to the other so we can better understand interior practice and its design process through the concept of affect. Finally, we conclude by addressing how the study of affect contributes to our understanding of interior practice and its design process, and more significantly, how, in exchange, might interior practice offer to recent theories of affect. Interior practice as changeable, spatio-temporal and material processes offer potentially fertile ground to explore affect as mediating layer, bridging human and non-human forces.