Complete genome sequence of Rhizobium leguminosarum bv trifolii strain WSM2304, an effective microsymbiont of the South American clover Trifolium polymorphum

Rhizobium leguminosarum bv. trifolii is the effective nitrogen fixing microsymbiont of a diverse range of annual and perennial Trifolium (clover) species. Strain WSM2304 is an aerobic, motile, non-spore forming, Gram-negative rod isolated from Trifolium polymorphum in Uruguay in 1998. This microsymbiont predominated in the perennial grasslands of Glencoe Research Station, in Uruguay, to competitively nodulate its host, and fix atmospheric nitrogen. Here we describe the basic features of WSM2304, together with the complete genome sequence, and annotation. This is the first completed genome sequence for a nitrogen fixing microsymbiont of a clover species from the American centre of origin. We reveal that its genome size is 6,872,702 bp encoding 6,643 protein-coding genes and 62 RNA only encoding genes. This multipartite genome was found to contain 5 distinct replicons; a chromosome of size 4,537,948 bp and four circular plasmids of size 4,537,948, 1,266,105, 501,946, 308,747 and 257,956 bp.

Regional variability in diving physiology and behavior in a widely distributed air-breathing marine predator, the South American sea lion Otaria byronia

Our understanding of how air-breathing marine predators cope with environmental variability is limited by our inadequate knowledge of their ecological and physiological parameters. Due to their wide distribution along both coasts of the sub-continent, South American sea lions (Otaria byronia) provide a valuable opportunity to study the behavioral and physiological plasticity of a marine predator in different environments. We measured the oxygen stores and diving behavior of South American sea lions throughout most of its range, allowing us to demonstrate that diving ability and behavior vary across its range. We found no significant differences in mass-specific blood volumes of sea lions among field sites and a negative relationship between mass-specific oxygen storage and size, which suggests that exposure to different habitats and geographical locations better explains oxygen storage capacities and diving capability in South American sea lions than body size alone. The largest animals in our study (individuals from Uruguay) were the most shallow and short duration divers, and had the lowest mass-specific total body oxygen stores, while the deepest and longest duration divers (individuals from Southern Chile) had significantly larger mass-specific oxygen stores, despite being much smaller animals.Our study suggests that the physiology of air-breathing diving predators is not fixed, but that it can be adjusted, to a certain extent, depending on the ecological setting and or habitat. These adjustments can be thought of as a "training effect" as the animal continues to push its physiological capacity through greater hypoxic exposure, its breath holding capacity increases.

The Permian brachiopod genus Trigonotreta Koenig and its occurrence in Argentina

A review of the Permian spiriferid brachiopod genus Trigonolrela and its occurrence in the Early Permian of Argentina is provided herein. Several species are known from the Late Palaeozoic sequences of the Argentinean Precordillera. These include Trigonolrela sp. and Trigonolrela riojanensis (Lech and Acenolaza), from the Rio del Peii.on Formation (Rio Blanco Basin), Trigonolrela pericoensis (Leanza), from the Tupe Formation at the La Herradura creek locality (Paganzo Basin) and Trigonolrela sanjuanensis (Lech and Acenolaza), from Del Salto Formation (Calingasta-Uspallata Basin). These species are characterised by being small to medium sized, relatively transverse, with cardinal extremities often strongly angular. Costae are weakly bifurcated and superimposed on weakly developed lateral flank plications adjacent to the fastigium and sulcus. The Argentinean species are close to the oldest known Indian species of the genus, Trigonotreta hesdoensis (Salmi and Dutt), particularly with respect to the nature of its weakly fasciculated costae. Further study will refine the details of the relationship of the South American species with those from elsewhere in Gondwana and may permit the recognition of a distinctive lineage. The presence of the genus in Argentina in the earliest Permian is an important palaeobiogeographical observation that raises questions about the probable migration routes of the genus from the western Gondwanan South American margin to eastern Australia and India. The Precordilleran region appears to be the likely site of the first appearance of Trigonolrela. Species with relatively simple costae appeared first. These gave rise to more complex species with a more elaborate costal pattern indicating an evolutionary progression through time.

Evidence from genetic and Lagrangian drifter data for transatlantic transport of small juvenile green turtles

Aim  A key life-history component for many animals is the need for movement between different geographical locations at particular times. Green turtle (Chelonia mydas) hatchlings disperse from their natal location to spend an early pelagic stage in the ocean, followed by a neritic stage where small juveniles settle in coastal areas. In this study, we combined genetic and Lagrangian drifter data to investigate the connectivity between natal and foraging locations. In particular we focus on the evidence for transatlantic transport. Location  Atlantic Ocean.

Methods  We used mitochondrial DNA (mtDNA) sequences (n = 1567) from foraging groups (n = 8) and nesting populations (n = 12) on both sides of the Atlantic. Genetic data were obtained for Cape Verde juvenile turtles, a foraging group not previously sampled for genetic study. Various statistical methods were used to explore spatial genetics and population genetic structure (e.g. exact tests of differentiation, Geneland and analysis of molecular variance). Many-to-many mixed stock analysis estimated the connectivity between nesting and foraging groups.

Results  Our key new finding is robust evidence for connectivity between a nesting population on the South American coast (25% of the Surinam nesting population are estimated to go to Cape Verde) and a foraging group off the coast of West Africa (38% of Cape Verde juveniles are estimated to originate from Surinam), thus extending the results of previous investigations by confirming that there is substantial transatlantic dispersal in both directions. Lagrangian drifter data demonstrated that transport by drift across the Atlantic within a few years is possible.

Main conclusions  Small juvenile green turtles seem capable of dispersing extensively, and can drop out of the pelagic phase on a transatlantic scale (the average distance between natal and foraging locations was 3048 km). Nevertheless, we also find support for the ‘closest-to-home’ hypothesis in that the degree of contribution from a nesting population to a foraging group is correlated with proximity. Larger-sized turtles appear to feed closer to their natal breeding grounds (the average distance was 1133 km), indicating that those that have been initially transported to far-flung foraging grounds may still be able to move nearer to home as they grow larger.

Queensland northern quolls are not immune to cane toad toxin

The release of the highly toxic South American cane toad (Bufo marinus) to the toad-free Australian continent in 1935, and their subsequent rapid spread over large areas of tropical Australia, has resulted in a massive decline of predators such as yellow-spotted goannas (Varanus panoptes) and northern quolls (Dasyurus hallucatus). In spite of dramatic declines of northern quoll populations in the Northern Territory, a few populations still persist in areas of Queensland where northern quolls have co-existed with toads for several decades

Increased mortality of naive varanid lizards after the invasion of non-native can toads (bufo marinus)

Exotic animal and plant species introduced into the Australian continent often imparted catastrophic effects on the indigenous fauna and flora. Proponents of biological control introduced the South American Cane Toad (Bufo marinus) into the sugar cane fields of Queensland in 1935. The Cane Toad is one of the most toxic bufonids and when seized by naive Australian predators, the toxin usually kills the attacker. One group of Australian squamate reptiles that are very susceptible to Cane Toad toxins is varanid lizards. Prior to Cane Toad invasion of our study area, the Adelaide River floodplain of the Northern Territory of Australia, annual mortality of adult male radio-tagged yellow-spotted Goannas (Varanus panoptes) was very low. After the arrival of toads in October 2005, all radio-tracked goannas were found dead in August 2006, most likely attempting to feed on the toads. Our results suggest that invasive Cane Toads place naive adult male Yellow-spotted Goannas at risk of possibly >90% mortality. This increase in mortality could reduce the genetic diversity and hamper long-term survival of these large carnivorous lizards.

Diving deeper into individual foraging specializations of a large marine predator, the southern sea lion

Despite global declines in the abundance of marine predators, knowledge of foraging ecology, necessary to predict the ecological consequences of large changes in marine predator abundance, remains enigmatic for many species. Given that populations suffering severe declines are of conservation concern, we examined the foraging ecology of southern sea lions (SSL) (Otaria flavescens)-one of the least studied otariids (fur seal and sea lions)-which have declined by over 90 % at the Falkland Islands since the 1930s. Using a combination of biologging devices and stable isotope analysis of vibrissae, we redress major gaps in the knowledge of SSL ecology and quantify patterns of individual specialization. Specifically, we revealed two discrete foraging strategies, these being inshore (coastal) and offshore (outer Patagonian Shelf). The majority of adult female SSL (72 % or n = 21 of 29 SSL) foraged offshore. Adult female SSL that foraged offshore travelled further (92 ± 20 vs. 10 ± 4 km) and dived deeper (75 ± 23 vs. 21 ± 8 m) when compared to those that foraged inshore. Stable isotope analysis revealed long-term fidelity (years) to these discrete foraging habitats. In addition, we found further specialization within the offshore group, with adult female SSL separated into two clusters on the basis of benthic or mixed (benthic and pelagic) dive behavior (benthic dive proportion was 76 ± 9 vs. 51 ± 8 %, respectively). We suggest that foraging specialization in depleted populations such as SSL breeding at the Falkland Islands, are influenced by foraging site fidelity, and could be independent of intraspecific competition. Finally, the behavioral differences we describe are crucial to understanding population-level dynamics, impediments to population recovery, and threats to population persistence.

Transitional experiences of international postgraduate students utilising a peer mentor programme

Background: Mentoring provides a range of benefits and one of them is social support. The number of students in transnational education has been increasing, and their transition into university is often fraught with difficulties. Universities can support transition through the use of a peer mentor programme (PMP).

Purpose: The purpose of the study is to investigate the transitional issues that international postgraduate students (IPSs) face in their transition to an Australian University. The study also investigated the role played by a mentoring programme, and how this assists students with their transition.

Sample: The sample included 31 IPSs, who had come from Asian European, Middle Eastern, African and South American countries, 15 being male, and 16 being female, with an average age of 24; most had been studying at the Australian University for more than 12 months.

Design and methods: The study utilised a qualitative research method to examine the experiences of IPSs undergoing transition from their home universities to an Australian university. Semi-structured interviews were conducted with students, who were asked about their transition experiences and the assistance of the mentor programme. Thematic analysis was then conducted to determine themes from the research.

Results: IPSs reported mixed experiences with their transition. Those with difficulties referred to loneliness, different studying practices, finding accommodation and making friends. IPSs reported that the PMP helped, as mentors provided the necessary social support, friendship, information and confidence to overcome those difficulties. Those students who did not have problems with their transition had reported that they had friends or family that assisted them with their transition. As a result, these students did not need the support of mentors to the same degree as those students having problems.

Conclusions: It is concluded that mentor programmes were important for transitioning IPSs who had difficulties. Therefore, this study identified a role for a PMP in universities where there are a high proportion of IPSs who are going through transitional problems.

Has China displaced its competitors in high tech trade?

This paper empirically investigated the extent to which China displaced its competitors in high-tech exports using disaggregated data for the period 1992–2013. To address the endogeneity problem, we used a comprehensive set of instruments for Chinese high-tech exports in relevant markets, including China's GDP and distances to those markets. Results of our IV regressions revealed that in most of the high-tech sectors, Chinese exports had displaced the exports of its developing competitors such as India, South American exporters like Brazil and Mexico, and South-East Asian countries like Malaysia, Singapore, Thailand and Vietnam, especially in the period prior to the 2007–08 global financial crisis. Yet, Chinese exports had been associated with more high-tech exports of developed exporters like OECD countries, South Korea and Japan. Our findings suggest that while China became the world's top high-tech exporter, its high-tech exported products had been substitutes to those of other developing and emerging economies but complementary to that of developed economies.