Grafts in myringoplasty : utilizing a silk fibroin scaffold as a novel device

Chronic perforations of the eardrum or tympanic membrane represent a significant source of morbidity worldwide. Myringoplasty is the operative repair of a perforated tympanic membrane and is a procedure commonly performed by otolaryngologists. Its purpose is to close the tympanic membrane, improve hearing and limit patient susceptibility to middle ear infections. The success rates of the different surgical techniques used to perform a myringoplasty, and the optimal graft materials to achieve complete closure and restore hearing, vary significantly in the literature. A number of autologous tissues, homografts and synthetic materials are described as graft options. With the advent and development of tissue engineering in the last decade, a number of biomaterials have been studied and attempts have been made to mimic biological functions with these materials. Fibroin, a core structural protein in silk from silkworms, has been widely studied with biomedical applications in mind. Several cell types, including keratinocytes, have grown on silk biomaterials, and scaffolds manufactured from silk have successfully been used in wound healing and for tissue engineering purposes. This review focuses on the current available grafts for myringoplasty and their limitations, and examines the biomechanical properties of silk, assessing the potential benefits of a silk fibroin scaffold as a novel device for use as a graft in myringoplasty surgery.

Surface energy of silk fibroin and mechanical properties of silk cocoon composites

Silkworm cocoons are biological composite structures protecting the silkworms against environmental damage and physical attack by natural predators. In particular, some outdoor reared silk cocoons exhibit outstanding mechanical properties that are relevant to the higher level protection required to enhance the survival chance of silkworms while supporting their metabolic activity. The performance of composite materials strongly depends on the adhesion between the fiber reinforcement and matrix, with the surface properties of the fibers playing a key role in determining the level of adhesion achieved. For this reason it is important to study the surface properties of silk fibroin to further understand the composite properties of the cocoons. In this work, both the mechanical properties of the silk cocoons and silk fibroin were studied. The surface topography was examined using scanning probe microscopy (SPM), which revealed distinct longitudinal ridges and striations along the fiber axis of the four silk fiber types. The fibers were found to exhibit heterogeneity in surface energy as evidenced from inverse gas chromatography (IGC) measurements. The combination of excellent mechanical properties and the more energetically heterogeneous surface nature of the wild A. pernyi silk fibroin fibers correlates well with the excellent mechanical properties of the A. pernyi cocoons. This journal is

Lessons from silkworm cocoons for future protective materials design

 Evolved over millions of years’ natural selection, very thin and lightweight wild silkworm cocoons can protect silkworms from environmental hazards and physical attacks from predators while supporting their metabolic activity. The knowledge of structure-property-function relationship of multi-layered composite silk cocoon shells gives insight into the design of next-generation protection materials. The mechanical and thermal insulation properties of both domestic (Bombyx mori, or B. moriand Samia. cynthia, or S. cynthia) and wild (Antheraea pernyi and Antheraea mylitta, or A. pernyi and A. mylitta) silkworm cocoons were investigated. The research findings are of relevance to the bio-inspired design of new protective materials and structures.
The 180 degree peel tests and needle penetration tests were used for examining the peel resistance and needle penetration resistance of both domestic and wild silkworm cocoon walls. The temperatures inside and outside of the whole silkworm cocoons under warm, cold and windy conditions were monitored for investigating the cocoon’s thermal insulation function. Computational fluid dynamics (CFD) models were created to simulate the heat transfer through the A. pernyi cocoon wall.
The wild cocoons experienced much higher peeling peak loads than the domestic cocoon. This transfers to a maximum work-of-fracture (WOF) of about 1000 J/m2 from the A. pernyi outer layer, which was 10 times of the B. mori cocoon. The A. pernyi wild cocoon exhibited a maximum penetration force (11 N) that is 70 % higher than a woven aramid fabric. Silk sericin is shown to play a critical role in providing needle penetration resistance of the non-woven composite cocoon structure by restricting the relative motion of fibres, which prevents the sharp tip of the needle from pushing aside fibres and penetrating between them. The wild A. pernyi cocoon exhibits superior thermal buffer over the domestic B. mori cocoon. The unique structure of the A. pernyi cocoon wall with mineral crystals deposited on the cocoon outer surface, can prohibit most of the air from flowing inside of the cocoon structure, which shows strong wind resistance under windy conditions.

Heat and moisture transfer through silkworm cocoon walls.

A silkworm cocoon is a biological porous structure which provides multiple protective functions. The knowledge from this natural protective system could contribute to develop advanced materials and structures with superior performances and meet the human demanding of novel breathable materials for enhanced thermal protection and comfort.