Tecnica simples de marcacao externa de reprodutores de tilapia nilotica (Oreochromis niloticus)

With the goal of organizing a control program of Nile tilapia (Oreochromis niloticus) reproduction in laboratory, a tecnique with ceramic pearls held on dorsal musculature by transfixion with flexible sintetic string was developed. Sixty fishes with 12g average weight and 60 with 19g average weight were used. Marking was done on three positions: frontal (F), median (M), and caudal (C). Different combinations of three collored pearls were fixed on the right side (defining number), and were linked to only one pearl in the left side (defining sex), with a slack of about 1.5cm to prevent growth damage. The animals were individually identified and weigthed at 30, 60 and 130 days after surgery. Results showed that both F and M positions allowed normal growth and reproductive behavior, whereas the C position induced mortality and loss of marker in some specimens. The M position is recomended as the best, or the intermediate between F and M positions.

Tecnica simples para identificacao de femeas de tilapia nilotica (Oreochromis niloticus) realizando incubacao oral dos ovos

In aquaculture laboratories which work with Nile tilapia (Oreochromis niloticus) reproduction and use crystalline water, is frequently necessary to verify whether the females are incubating orally the eggs. The identification of the females when there is not know-how is difficult and slow, and in most cases is stressful and damage the eggs. This study describes a simple technique, using a mirror, to identify Nile tilapia incubating eggs orally in crystalline water. The results showed that is possible to identify the females which are incubating orally with 100% efficiency.

Validation of the aceto-carmine technique for evaluating phenotypic sex in Nile tilapia (Oreochromis niloticus) fry

In this study a method for evaluating phenotypic sex in Nile tilapia was validated. A technique that uses aceto-carmine squash mounts to stain the entire gonadal tissue for microscopic examination (Method 2- squash mounts) was compared with a technique based on traditional histology. Approximately 2600 Nile tilapia fry weighing and measuring, respectively, between 0.25-2.50g and 26-53mm, aged 35 to 60 days after hatch (DAH), were sexed using this methodology. In situ microscopic examination on the gonads was also performed. A reliable sexing using squash mount was possible with fish weighing more than 0.500g, 45-47 DAH. Results from microscopic observation using the aceto-carmine stain coincided 100% with the histological examination. Male gonadal tissue was characterized by the presence of cysts containing spermatogonia and spermatocytes, while females were easily identified by the presence of oocytes at the perinucleolar stage. The technique proved to be efficient not only in terms of evaluating sex proportion in fish but also because it allows immediate evaluation of gonadal sex and demands less time and labour.

Ocupação, bem-estar e satisfação de vida: a experiência de um modelo alternativo de estágio em terapia ocupacional na Austrália

An alternative training model within occupational therapy developed at Deakin University, in Australia, named Occupation, Wellness and Life-Satisfaction (OWLS) is presented. OWLS provides students with opportunities to practice in nontraditional settings, thus enabling strategic learning in non-clinical settings, including working with professionals who are not occupational therapists and with different work cultures and practices, in response to gaps identified within the community. It promotes opportunities for developing skills such as health promotion, health education and assessment and interventions in schools, thus improving the health and wellbeing of the community. The program has contributed towards development of new projects, with positive results achieved by students, thereby representing an important educational strategy for professional occupational therapy training and expanding the field of activities.