The influence of age on reproductive success and diet in Australasian gannets (Morus Serrator) breeding at Pope's Eye, Port Phillip Bay, Victoria

The influence of age on reproductive success and diet was examined in ‘old’ (experienced; 12 years and older) and ‘young’ (5–8 years of age) Australasian gannets (Morus serrator) breeding at Pope’s Eye, Port Phillip Bay, Victoria during the 2002–2003 breeding period. Although food availability, as indicated by commercial fish catches, throughout this breeding period was low, there were no significant differences in breeding success or chick growth between groups. Nevertheless, old birds tended to have higher reproductive success, replacing more lost eggs and fledging chicks of a greater mass. However, old birds also laid more eggs that failed to hatch. Five fish species, including jack mackerel (Trachurus declivis), barracouta (Thyrsites atun), redbait (Emmelichthys nitidus), anchovy (Engraulis australis) and red mullet (Upeneichthys vlamingii), were important in the gannet diet during this breeding period. There were no significant differences in dietary parameters, including range of species and size of prey, between old and young gannets, nor were there any differences between those of the chicks and their parents, suggesting that adults do not forage selectively for their chicks. This study showed that even during a period of presumed low food availability, when experienced (older) birds might be expected to have enhanced success, the differences between these and less experienced (younger) birds may not be apparent.

Long-term reproductive success of Australasian gannets (Morus serrator)

Life history theory predicts that individuals make trade-offs between investment in current reproduction, future reproductive potential and success in order to maximise lifetime reproductive success (LRS). Factors that may influence LRS in seabirds include delayed maturity, small clutches, high adults survivorship and long life spans. Studies have shown that reproductive success of seabirds increases with age and experience. Australasian gannets are long-lived seabirds that typically commence breeding at five or six years of age. A small colony of gannets was monitored over 12 years. This study aimed to identify patterns of long-term individual breeding biology. Results indicate that nest site, pair bond duration, age and breeding experience are all important factors in reproductive success. Relationships were found between breeding success and several variables including breeding experience, pair bond duration and nest site quality. Identifying and understanding the factors that contribute to individuals’ reproductive success is of critical importance for future conservation and management of seabird species.

Dispersal of Hormosira banksii (phaeophyceae) via detached fragments : reproductive viability and longevity

Drifting, fertile thalli are well documented to be the primary long-distance dispersal vector for many marine macroalgae, but little information about reproductive viability of drift is known. This study examined the reproductive viability and longevity of floating fragments of the intertidal Australasian fucoid Hormosira banksii (Turner) Decne. Beach wrack surveys and field experiments were conducted to test the model that long-distance dispersal is achieved in H. banksii via floating, fertile fronds. High densities of beach wrack fragments were evident during summer compared to autumn. The majority of beach wrack occurred on sandy beaches rather than rocky shores. Both male and female fragments were present in the beach wrack. Detached fronds were capable of releasing gametes up to 8 weeks after detachment. Beach wrack produced high fertilization rates and recruited successfully onto artificial panels. Results suggest that detached fragments are reproductively viable and that floating, fertile fronds may be an important mechanism for facilitating long-distance dispersal in this species. Nevertheless, the frequency of fronds reaching a suitable habitat and contributing to gene flow between populations, or colonizing new populations, may not be proportional to the total density of beach wrack.

Reproductive biology of the eastern shovelnose stingaree Trygonoptera imitata from south-eastern Australia

Abstract. In applying a quantitative approach to the reproduction of Trygonoptera imitata, the present study contributes to understanding the wide diversity in the reproductive biology of the family Urolophidae and provides insights to help determine phylogenetic relationships. This localised species is taken as bycatch in several inshore fisheries and potentially impacted by a range of other anthropogenic pressures, including introduced species, particularly in shallow-water pupping areas.T. imitata can be characterised as a species of comparatively lowmatrotrophic histotrophy with an extended period of relatively large eggs in utero (5–8 months) followed by rapid growth of the embryos (4–6 months). The reproductive cycle is annual with parturition occurring during late-February–April, followed immediately by ovulation. Mean size-at-birth is ~225mm total length and there is a ~1000% gain in mean wet mass from egg (15 g) to full-term embryo in utero (150 g), the lowest reported for any viviparous batoid. Litter size increases with maternal length, reaching a maximum of seven, and sex ratio of embryos is 1 : 1. Maximum length and estimates of the maturity–ogive parameters l₅₀ and l₉₅ are similar for females and males.

Comparative distribution of a putative egg-laying hormone in neural and reproductive tissues of four Decapoda crustaceans

Evidence for the presence of a putative egg-laying (ELH) hormone has been previously described in the black tiger shrimp, Penaeus monodon, so a further investigation was carried out to detect its presence in a range of Decapoda crustaceans prior to a full molecular analysis. The crustaceans were represented by the Australian fresh water yabbie, Cherax destructor, the Australian southern rock lobster, Jasus edwardsii, the snow crab, Chionoecetes opilio, and the blue swimmer crab, Portunus pelagicus. Female cerebral ganglia, ventral nerve cords and gonads were investigated in a comparative study of the distribution of the immunoreactive hormone using immunoenzyme and immunofluorescence techniques. Immunoreactivity was detected in all tissues of interest, and the distribution patterns showed similarity within the four species, as well as that of P. monodon reported in the earlier study. There were minor variations. These data indicate that a putative ELH-like neuropeptide is widespread in crustaceans, and supports its previous identification in a range of molluscs and other invertebrates. Elucidation of the molecular structure of the peptide hormone and its encoding gene, as well as its involvement in spawning behaviour of crustaceans, is now fully under investigation.

Disorganized attachment and reproductive strategies

Del Giudice provides an extension of the life history theory of attachment that incorporates emerging data suggestive of sex differences in avoidant male and preoccupied female attachment patterns emerging in middle childhood. This commentary considers the place of disorganized attachment within this theory and why male children may be more prone to disorganized attachment by drawing on Trivers’s parental investment theory.

Do reproductive tactics vary with habitat heterogeneity in the intertidal sea anemone Actinia tenebrosa?

Cnidarians display a diverse range of reproductive tactics including sexual and asexual modes of reproduction. Although few studies have looked for intraspecific variation in reproductive tactics, flexible expression of such life-history traits may be favoured in species that occupy a range of habitats. We tested this in the sea anemone Actinia tenebrosa by comparing cycles of reproductive activity and the mode of production of brooded larvae in local populations occupying boulder fields and stable rock platforms. We determined the mode of production of broods from eight rock platforms (separated by up to 1600 km) and two boulder shores on the south east coast of Australia using a combination of allozyme data and four newly characterised microsatellite markers.

We determined seasonal patterns of brooding and gonad development by monthly dissection of 15–30 adults from each of two boulder fields and two stable platforms. Previous genetic studies have shown that populations of A. tenebrosa on rock platforms can be highly clonal, whereas anemones on more heterogeneous boulder habitats display levels of genotypic diversity similar to that expected for sexual reproduction. We genotyped a total of 221 juveniles from 37 brooding adults including 11 broods and 80 juveniles from boulder shores. We did not detect any evidence of sexual production of broods. All brooded juveniles displayed identical multi-locus genotypes to their brood parent irrespective of habitat of origin or location, including 28 broods (200 juveniles) that were heterozygous at one or more locus. Similarly, we found that temporal patterns of gonad formation and brooding were consistent across habitats and locations. We detected 346 mature males, 234 non-reproductive or immature individuals, and no mature females within a total of 580 dissected individuals. These data suggest that the reproductive tactics of A. tenebrosa are essentially fixed and that variation in the genotypic diversity of populations may reflect variation in factors such as the input of sexually derived planktonically dispersed recruits or post-settlement processes. However, the apparent lack of females paradoxically implies that sexual reproduction, and hence recruitment, must be rare or no longer possible within some populations, and highlights the need for long-term studies of these populations.

Microscopic modifications of the female reproductive tissues of Mustelus antarcticus

The ovarian follicles and oviducal glands have structural organisations similar to other chondrichthyans. Sperm are stored in the oviducal gland of all maturing and mature animals throughout the year and throughout pregnancy. Microscopic features of the uterine epithelium suggest nutrients are supplied to developing embryos without placenta formation.

Reproductive performance and egg and larval quality of the blacklip abalone

The study highlights the interrelationship between physical characteristics of female broodstock with reproductive performance, egg quality and subsequent larval success, and reproductive seasonality of the blacklip abalone (Haliotis rubra Leach). Suitable indices for assessing broodstock were developed. Seasonal changes in biochemical compositions in relation to maturation and development was discussed.

Interannual variation in pup production and the timing of breeding in benthic foraging Australian fur seals

¯Large interannual variations in reproductive success caused by fluctuations in oceanography and prey availability are common to many species of air breathing epipelagic predators. In contrast, little is known about variation in benthic foragers such as Australian fur seals (Arctocephalus pusillus doriferus). Between 1997 and 2007, pup production was assessed in 9 yr, while the timing of breeding and adult female condition was assessed in 5 yr at Kanowna Island in Bass Strait, southeastern Australia. Pup production was variable ( ¯x = 1,726 ± 42, range = 1,386–2,301), but without temporal trend, as was median birth date ( ¯x = 23 November±1, range = 21–25 November) and pupping synchrony (period of 90% births: ¯x = 28 ± 2 d, range = 23–31 d). Pup production was negatively correlated with median birth date and positively correlatedwith female condition,winter sea-surface temperature (SST) and zonal wind strength within Bass Strait. Pup production was also negatively correlated with SST in the previous summer within Bass Strait and in the eastern Great Australian Bight upwelling region. The results suggest that the reproductive success of Australian fur seals is influenced by oceanography but less so than in otariids foraging epipelagically in major upwellings. Despite spanning several El Niño events, no correlation between pup production and the Southern Oscillation Index was observed.

Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone

In molluscs, the neurotransmitter serotonin (5-HT) has been linked to a variety of biological roles including gamete maturation and spawning. The possible involvement of 5-HT in abalone gamete release was demonstrated by a dose-dependent increase in Haliotis rubra gonad contractile bioactivity following 5-HT stimulation. Physiological functions associated with 5-HT, are mediated through binding to 5-HT receptors. A cDNA encoding a putative 5-HT receptor consisting of 359 amino acids was isolated from the tropical abalone H. asinina, termed 5-HT_{1 ha}. The 5-HT_{1 ha} shares G-protein-coupled receptor motifs with metazoan 5-HT receptors, including predicted transmembrane domains, active sites for protein kinase action, and N-linked glycosylation sites. However, the third intracellular loop of 5-HT_{1 ha} is relatively short, and only six transmembrane domains are predicted, implying a truncated receptor. Phylogenetic analysis with known 5-HT receptor genes suggests that 5-HT_{1 ha} belongs to the type 1 5-HT receptor family. Expression analysis by RT-PCR showed that 5-HT_{1 ha}  mRNA was present in all tissues examined, including the neural ganglia and gonad tissues. Immunocytochemistry revealed the presence of 5-HT_{1 ha} specifically within the soma of neuronal cells located in the outer cortex of both cerebral and pleuropedal ganglia. In ovarian and testicular tissues, 5-HT_{1 ha} immunoreactivity was observed in epithelial cells of the outer capsule and connective tissue of the trabeculae to which the gamete follicles adhere. Whether this receptor transcript is translated to a functional protein needs to be verified, but if so, it could play a role in reproduction.