Suppression of bovine viral diarrhea virus replication by small interfering RNA and short hairpin RNA-mediated RNA interference

Bovine viral diarrhea virus (BVDV) is a ubiquitous viral pathogen that affects cattle herds’ worldwide causing significant economic loss. The current strategies to control BVDV infection include vaccination (modified-live or killed) and control of virus spread by enhanced biosecurity management, however, the disease remains prevalent. With the discovery of the sequence-specific method of gene silencing known as RNA interference (RNAi), a new era in antiviral therapies has begun. Here we report the efficient inhibition of BVDV replication by small interfering (siRNA) and short hairpin RNA (shRNA)-mediated gene silencing. siRNAs were generated to target the 5′ non-translated (NTR) region and the regions encoding the C, NS4B and NS5A proteins of the BVDV genome. The siRNAs were first validated using an EGFP/BVDV reporter system and were then shown to suppress BVDV-induced cytopathic effects and viral titers in cell culture with surprisingly different activities compared to the reporter system. Efficient viral suppression was then achieved by bovine 7SK-expressed BVDV-specific shRNAs. Overall, our results demonstrated the use of siRNA and shRNA-mediated gene silencing to achieve efficient inhibition of the  replication of this virus in cell culture.

Growth and replication : exploring facade subdivision based on natural processes

Generative methods for subdivision of building façades are presented in this thesis through a series of case studies exploring methods of stochastic generation, tiling and tessellation and branching structures.  In each of these studies, a method of generative design based on natural phenomena of growth and replication is described algorithmically.

‘Fat, four-eyed and female’ 30 years later: A replication of Harris, Harris, and Bochner’s (1982) early study of obesity stereotypes

Objective: This study aimed to replicate Harris, Harris, and Bochner’s (1982) early experiment on obesity stereotyping to examine whether negative obesity stereotypes persist and in what form.

Method: A sample of psychology students (N = 506) read a description of a target described as female or male, overweight or average weight, and wearing glasses or not, who they subsequently rated on 12 descriptors.

Results: Overweight targets were rated as significantly less active, assertive, athletic, attractive, happy, hardworking, masculine, popular, and successful than average weight targets. This negative stereotype effect of target weight was much larger than effects observed for sex or wearing glasses. There were no differences in effect sizes for target weight between this study and the original study.

Conclusions: It was concluded that the negative obesity stereotypes reported by Harris et al. have persisted over a 30-year period, despite the fact that people who are overweight are no longer a minority. Efforts are needed to challenge negative stereotyping of this group. Future research could examine why stereotypes of overweight people are resistant to change.