Possible changes of pore size during nanofiltration

The textile industry plays an important role in the world economy as well as our daily life. However, the industry consuming a large quantity of water and generating huge amount of wastewater are unsustainable to the conservation of our precious resources and environment and need improvement. The wastewater, especially the one from spent cotton reactive dyebaths, contains high salt content, various dyes and high alkalinity. This study was carried out to investigate the feasibility of membrane filtration treating spent cotton reactive dye baths. A stirred cell with nanofiltration membrane was used aiming at reusing the reclaimed water. Spent dyebath solutions were synthesized containing hydrolyzed C. I. Reactive Black 5 and sodium chloride. When a piece of membrane was used repeatedly it was expected the flux would decrease after each usage due to fouling of impurities. However, it was found that the water flux increased while dye rejection decreased after each run. At pH 10, the dye rejection decreased significantly. It was proposed that the pore sizes of membrane might have changed during membrane filtration. An equation was derived calculating the possible changes of pore sizes.

Identification of a putative egg-laying hormone in neural and ovarian tissues of the black tiger shrimp, Penaeus monodon, using immunocytochemistry

The existence of an egg-laying hormone (ELH) was identified for the first time in the black tiger shrimp, Penaeus monodon, by means of immunoenzyme and immunofluorescence techniques. This was achieved using a polyclonal antibody produced against expressed recombinant ELH of the female Australian blacklip abalone, Haliotis rubra. The shrimp ELH reactive material was found to be localised within female neurosecretory tissues and the secretory tissue of the antennal gland, but was not identified in the X-organ sinus gland within the eyestalk. It was also present in the ovary, where the amount of ELH present was observed to be greatest in the period prior to spawning. These findings implied that the induction of P. monodon spawning might be involved with humoral regulation relating to ELH expression.

Primary sex ratio bias in an endangered cooperatively breeding bird, the Black-eared Miner, and its implications for conservation

The aim of our study was to investigate primary and adult sex ratios in the cooperatively breeding black-eared miner, Manorina melanotis. We used genetic methods to determine the sex of all birds. Observations were made to quantify differences in helping behaviour between the sexes. As in other miners, Manorina spp., non-breeding males provided most of the help in raising young. Male and female nestlings did not differ significantly in weight, suggesting that both sexes are equally costly to produce. Like other miners, the adult sex ratio in black-eared miners is male-biased (64.4%). However, unlike its congeners, the black-eared miner’s primary sex ratio was strongly biased toward females (62.5%). This suggests that females suffer higher juvenile mortality than males. Our study illustrates how understanding sex ratios is both of theoretical interest and relevant to biological conservation.

Black fella land – white fella tax : changing the CGT implications of aboriginal/native title

The taxation of aboriginal/native title payments gives rise to a number of complex and difficult legal and policy issues. Reform measures announced on 13 February 1998 by the then Federal Treasurer and Attorney-General did not address the possible capital gains tax (‘CGT’) implications and even those relating to ordinary income under s 6-5 Income Tax Assessment Act 1997 (Cth) remain unimplemented. The much anticipated Report of the Native Title Payments Working group (6 February 2009), while primarily focusing on non-taxation issues, also recognises the need for taxation reform and makes some recommendations in regard to such. Most recently, on 18 May the Assistant Treasurer, Senator Nick Sherry, the Minister for Families, Housing, Community Services and Indigenous Affairs, Jenny Macklin, and the Attorney General, Robert McClelland, announced the commencement of a national consultation on the tax treatment of native title, including the interaction of native title, Indigenous economic development and the tax system. The Assistant Treasurer recognised the need for “greater clarity and increased certainty for native title holders on how the tax system and native title interact.” At the same time, they released a paper entitled Native Title, Indigenous Economic Development and Tax to guide the national consultation. The proposed measures considered in the paper, including exempting Native title payments and/or creating a new tax exempt Indigenous Community Fund, provide a welcome step towards reform in this area. This article is part of a broader research project that explores the CGT implications of aboriginal/native title. While these provisions impact on both Indigenous traditional owners and relevant payers, such as mining companies, the focus in the project is particularly on the CGT implications for the traditional owners. This first part of the project examines the status of aboriginal/native title and incidental/ ancillary rights as CGT assets. The broader research project will then build on this analysis in the context of relevant CGT events. As the preliminary findings in this article evidence the CGT implications of aboriginal/native title are far from certain. The application of CGT to aboriginal/native title raises more issues than it answers. The key reason is that the current law is entirely unsuitable to communally held inalienable aboriginal/native title. Nevertheless, it will be seen that it is arguable that aboriginal/native title and/or incidental rights are post-CGT assets and acts in relation to such could trigger a CGT event with tax implications for the traditional owners. It will be suggested that these current tax provisions provide a very pertinent example where the law operates as a blunt tool that does not appropriately promote justice and reconciliation. To tax Indigenous communities as a result of acts that extinguish or impair their traditional ownership is incongruous. A specific provision(s) should be included in the capital gains provisions to ensure any such payments are exempt from taxation. This is not only fair given the history of uncompensated extinguishment of aboriginal title Australia, but also promotes the ability of Indigenous communities to optimise the financial benefits stemming from aboriginal/native title agreements.

Concentrations of major grass group 5 allergens in pollen grains and atmospheric particles : implications for hay fever and allergic asthma sufferers sensitized to grass pollen allergens

Background

Grass pollen allergens are the most important cause of hay fever and allergic asthma during summer in cool temperate climates. Pollen counts provide a guide to hay fever sufferers. However, grass pollen, because of its size, has a low probability of entering the lower airways to trigger asthma. Yet, grass pollen allergens are known to be associated with atmospheric respirable particles.
Objective

We aimed (1) to determine the concentration of group 5 major allergens in (a) pollen grains of clinically important grass species and (b) atmospheric particles (respirable and nonrespirable) and (2) to compare the atmospheric allergen load with clinical data to assess different risk factors for asthma and hay fever.
Methods

We have performed a continuous 24 h sampling of atmospheric particles greater and lower than 7.2 μm in diameter during the grass pollen season of 1996 and 1997 (17 October 1996–16 January 1997) by means of a high volume cascade impactor at a height of about 15 m above ground in Melbourne. Using Western analysis, we assessed the reactivity of major timothy grass allergen Phl p 5 specific monoclonal antibody (MoAb) against selected pollen extracts. A MoAb-based ELISA was then employed to quantify Phl p 5 and cross-reactive allergens in pollen extracts and atmospheric particles larger and smaller than 7.2 μm.
Results

Phl p 5-specific MoAb detected group 5 allergens in tested grass pollen extracts, indicating that the ELISA employed here determines total group 5 allergen concentrations. On average, 0.05 ng of group 5 allergens were detectable per grass pollen grain. Atmospheric group 5 allergen concentrations in particles > 7.2 μm were significantly correlated with grass pollen counts (rs = 0.842, P < 0.001). On dry days, 37% of the total group 5 allergen load, whereas upon rainfall, 57% of the total load was detected in respirable particles. After rainfall, the number of starch granule equivalents increased up to 10-fold; starch granule equivalent is defined as a hypothetical potential number of airborne starch granules based on known pollen count data. This indicates that rainfall tended to wash out large particles and contributed to an increase in respirable particles containing group 5 allergens by bursting of pollen grains. Four day running means of group 5 allergens in respirable particles and of asthma attendances (delayed by 2 days) were shown to be significantly correlated (P < 0.001).
Conclusion

Here we present, for the first time, an estimation of the total group 5 allergen content in respirable and nonrespirable particles in the atmosphere of Melbourne. These results highlight the different environmental risk factors for hay fever and allergic asthma in patients, as on days of rainfall following high grass pollen count, the risk for asthma sufferers is far greater than on days of high pollen count with no associated rainfall. Moreover, rainfall may also contribute to the release of allergens from fungal spores and, along with the release of free allergen molecules from pollen grains, may be able to interact with other particles such as pollutants (i.e. diesel exhaust carbon particles) to trigger allergic asthma.

The effect of cocoa supplementation on hepatic steatosis, reactive oxygen species and LFABP in a rat model of NASH

Background: Non alcoholic steatohepatitis is hypothesised to develop via a mechanism involving fat accumulation and oxidative stress. The current study aimed to investigate if an increase in oxidative stress was associated with changes in the expression of liver fatty acid binding protein in a rat model of non alcoholic steatohepatitis and whether cocoa supplementation attenuated those changes.

Methods: Female Sprague Dawley rats were fed a high fat control diet, a high fat methionine choline deficient diet, or one of four 12.5% cocoa supplementation regimes in combination with the high fat methionine choline deficient diet.

Results: Liver fatty acid binding protein mRNA and protein levels were reduced in the liver of animals with fatty liver disease when compared to controls. Increased hepatic fat content was accompanied by higher levels of oxidative stress in animals with fatty liver disease when compared to controls. An inverse association was found between the levels of hepatic liver fatty acid binding protein and the level of hepatic oxidative stress in fatty liver disease. Elevated NADPH oxidase protein levels were detected in the liver of animals with increased severity in inflammation and fibrosis. Cocoa supplementation was associated with partial attenuation of these pathological changes, although the severity of liver disease induced by the methionine choline deficient diet prevented complete reversal of any disease associated changes. Red blood cell glutathione was increased by cocoa supplementation, whereas liver glutathione was reduced by cocoa compared to methionine choline deficient diet fed animals.

Conclusion: These findings suggest a potential role for liver fatty acid binding protein and NADPH oxidase in the development of non alcoholic steatohepatitis. Furthermore, cocoa supplementation may have be of therapeutic benefit in less sever forms of NASH.

Timing of breeding and diet of the Black-faced cormorant Phalacrocorax fuscescens

Previous anecdotal reports have suggested that Black-faced Cormorant Phalacrocorax fuscescens breeds only in winter in southeastern Australia, but detailed reports confirming this are lacking. Here we examine the timing of breeding in Black-faced Cormorants at Notch Island in northern Bass Strait in 2006. Peak laying occurred during winter (ca 26 July). The diet of Black-faced Cormorants was predominantly fish (97% of identified prey) and varied between breeding and post-breeding periods. Black-faced Cormorants consumed a total of 14 different species with four species having a frequency of occurrence in the diet of ?5% during the breeding season and six species during the post-breeding period. We provide data for the first time on the chronology of breeding of Black-faced Cormorants in one year and give a preliminary description of their diet based on pellet analyses. We propose that late winter breeding may be a strategy to avoid the high ambient temperatures in northern Bass Strait during summer, the associated higher thermoregulatory costs for adults and the increased mortality for chicks.

Reasoned versus reactive prediction of behaviour: a meta-analysis of the prototype willingness model

The prototype willingness model (PWM) was designed to extend expectancy-value models of health behaviour by also including a heuristic, or social reactive pathway, to better explain health-risk behaviours in adolescents and young adults. The pathway includes prototype, i.e., images of a typical person who engages in a behaviour, and willingness to engage in behaviour. The current study describes a meta-analysis of predictive research using the PWM and explores the role of the heuristic pathway and intentions in predicting behaviour. Eighty-one studies met inclusion criteria. Overall, the PWM was supported and explained 20.5% of the variance in behaviour. Willingness explained 4.9% of the variance in behaviour over and above intention, although intention tended to be more strongly related to behaviour than was willingness. The strength of the PWM relationships tended to vary according to the behaviour being tested, with alcohol consumption being the behaviour best explained. Age was also an important moderator, and, as expected, PWM behaviour was best accounted for within adolescent samples. Results were heterogeneous even after moderators were taken into consideration. This meta-analysis provides support for the PWM and may be used to inform future interventions that can be tailored for at-risk populations.

Effects of visual contact with zoo visitors on black-capped capuchin welfare

Previous research has suggested that the presence of zoo visitors may be stressful for various primate species, and visual contact with visitors may be the sensory stimuli that mediate visitor effects. We studied a group of black-capped capuchins, Cebus apella, in a controlled experiment, randomly imposing two treatments: customised one-way vision screens on the exhibit viewing windows to reduce visual contact with visitors; and unmodified viewing windows that allow full visual contact with visitors. We sampled capuchin behaviour including intra-group aggression and other social interactions, vigilance and abnormal behaviours. To provide a measure of physiological stress, we also analysed capuchin faecal samples for glucocorticoid metabolite (FGM) concentration. When the view of visitors was obscured, we found marked reductions in capuchin aggression (from 14.5 bouts to 4.6 bouts per weekend, P= 0.004) and FGM concentration (from 620 to 410. ng/g, P= 0.008) among all adults, as well as reductions in abnormal behaviour (P = 0.01) in two individuals. The capuchins also avoided the visitor viewing area (P = 0.003) in the unmodified viewing window treatment. These results suggest that reducing the capuchins' ability to view visitors improved their welfare. However we also found a reduction in the number of visitors when visual contact was reduced (from an average of 23 visitors per scan to 15, P = 0.008), suggesting that the visitor experience may have been compromised by the lack of interaction with the capuchins. These results highlight a possible dilemma for the zoo industry between enhancing animal welfare in primates and providing for visitor experience.

Pro-inflammatory dietary intake as a risk factor for CVD in men: a 5-year longitudinal study

Convincing evidence has identified inflammation as an initiator of atherosclerosis, underpinning CVD. We investigated (i) whether dietary inflammation, as measured by the 'dietary inflammatory index (DII)', was predictive of 5-year CVD in men and (ii) its predictive ability compared with that of SFA intake alone. The sample consisted of 1363 men enrolled in the Geelong Osteoporosis Study who completed an FFQ at baseline (2001-2006) (excluding participants who were identified as having previous CVD). DII scores were computed from participants' reported intakes of carbohydrate, micronutrients and glycaemic load. DII scores were dichotomised into a pro-inflammatory diet (positive values) or an anti-inflammatory diet (negative values). The primary outcome was a formal diagnosis of CVD resulting in hospitalisation over the 5-year study period. In total, seventy-six events were observed during the 5-year follow-up period. Men with a pro-inflammatory diet at baseline were twice as likely to experience a CVD event over the study period (OR 2·07; 95 % CI 1·20, 3·55). This association held following adjustment for traditional CVD risk factors and total energy intake (adjusted OR 2·00; 95 % CI 1·03, 3·96). This effect appeared to be stronger with the inclusion of an age-by-DII score interaction. In contrast, SFA intake alone did not predict 5-year CVD events after adjustment for covariates (adjusted OR 1·40; 95 % CI 0·73, 2·70). We conclude that an association exists between a pro-inflammatory diet and CVD in Australian men. CVD clinical guidelines and public health recommendations may have to expand to include dietary patterns in the context of vascular inflammation.

Decolorization and mineralization of an azo reactive dye using loaded nano-photocatalysts on spacer fabric: kinetic study and operational factors

In this study, the photocatalytic decolorization and mineralization of Remazol Black B (RBB), an azo reactive dye, in aqueous solutions was investigated using UV/H2O2/ZnO, UV/H2O2/TiO2 and UV/H2O2/ZnO:TiO2 systems. ZnO and TiO2 nanoparticles were loaded on 3-dimensional polyethylene terephthalate fabrics (spacer fabrics). Morphology of the spacer fabrics and the presence of the nanoparticles were studied by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS), respectively. Furthermore, the effects of key operational parameters on the efficiency of the decolorization were investigated. These parameters included initial pH value, initial hydrogen peroxide concentration, initial dye concentration, the loaded nanoparticle ratio and the presence of anions (sulfate, chloride and bicarbonate). Zero-, first- and second-order reaction kinetics were evaluated. Complete decolorization and high efficient mineralization with 90% total organic carbon (TOC) reduction were achieved at 120min treatment in the case of ZnO:TiO2 under optimum condition. The results proved that the novel heterogeneous photocatalytic process is capable of decolorizing and mineralizing azo reactive dyes in textile wastewater.