Characterisation of a synthesised fluorescent ligand (4-acridinol-1-sulphonic acid) using nuclear magnetic resonance spectroscopy

The synthesis and complete characterisation of the fluorescent ligand, 4-acridinol-1-sulphonic acid (the acridine analogue of 8-quinolinol-5-sulfonic acid) is described. Using a judicious array of nuclear magnetic resonance spectroscopy experiments, the structural elucidation and full assignment of all proton and carbon chemical shifts were afforded. The 4-acridinol-1-sulphonic acid was found to behave in a similar manner to 8-quinolinol-5-sulphonic acid, forming fluorescent complexes with magnesium(II) and zinc(II). The uncorrected emission maxima for the metal–acridinol complexes were found to be at around 620 nm compared to 505 nm for the respective quinolinol complexes. Unfortunately, preliminary spectrofluorimetric analytical figures of merit revealed that the detection limits of the new acridinol metal complexes were one and a half orders of magnitude poorer than those attained with the corresponding quinolinol ligand. However, in contrast to 8-quinolinol-5-sulphonic acid, the 4-acridinol-1-sulphonic acid ligand showed considerable selectivity for magnesium(II) and zinc(II) over aluminium(III).

Exercise increases nuclear AMPK α2 in human skeletal muscle

An acute bout of exercise increases skeletal muscle glucose uptake, improves glucose homeostasis and insulin sensitivity, and enhances muscle oxidative capacity. Recent studies have shown an association between these adaptations and the energy-sensing 5' AMP-activated protein kinase (AMPK), the activity of which is increased in response to exercise. Activation of AMPK has been associated with enhanced expression of key metabolic proteins such as GLUT-4, hexokinase II (HKII), and mitochondrial enzymes, similar to exercise. It has been hypothesized that AMPK might regulate gene and protein expression through direct interaction with the nucleus. The purpose of this study was to determine if nuclear AMPK α₂ content in human skeletal muscle was increased by exercise. Following 60 min of cycling at 72 +/- 1% of V_O2peak in six male volunteers (20.6 +/- 2.1 years; 72.9 +/- 2.1 kg; V_O2peak = 3.62 +/- 0.18 l/min), nuclear AMPK α₂ content was increased 1.9 +/- 0.4-fold (P = 0.024). There was no change in whole-cell AMPK α₂ content or AMPK α₂ mRNA abundance. These results suggest that nuclear translocation of AMPK might mediate the effects of exercise on skeletal muscle gene and protein expression.

The nuclear exclusion : what does it mean and who has been tampering with it?

A nuclear exclusion appears in all general insurance policies. Since its introduction to Australia and New Zealand in the 1960s this exclusion has seen almost no change. So what are the reasons for this article? There are two reasons. First, there has been a misunderstanding on the part of some in the industry about the scope of this exclusion. This results in unnecessary alterations to the policy. The other is that a new wording is emerging some sections of the market which could be tar-reaching in its effect. The purpose of this article is to examine several aspects related to the exclusion. The first section examines the nature and extent of exposures in relation to radiation and nuclear energy and serves as background to under standing the exclusion wording. Section two provides the reasons for the inclusion of the clause and its historical origins. Section three addresses the intended scope of the current exclusion and the final section examines the scope of a new wording that is appearing and the possible implications that may result.

Studies on the mechanism of the peroxyoxalate chemiluminescence reaction Part 1. Confirmation of 1,2-dioxetanedione as an intermediate using 13C nuclear magnetic resonance spectroscopy

A simple model peroxyoxalate chemiluminescence system was monitored directly across a range of temperatures (from −80 to +20 °C) using ¹³C nuclear magnetic resonance spectroscopy. These experiments were made possible by the utilisation of ¹³C doubly labelled oxalyl chloride, which was reacted with anhydrous hydrogen peroxide in dry tetrahydrofuran. Ab initio quantum calculations were also performed to estimate the ¹³C nuclear magnetic resonance (NMR) shift of the most commonly postulated key intermediate 1,2-dioxetanedione and this data, in concert with the spectroscopic evidence, confirmed its presence during the reaction.

Employer matters in 2003

The year 2003 was characterised by employer proactivism, and a preparedness to pursue new legal manoeuvres to prevent or terminate protected industrial action. A number of employers also resorted to lengthy lockouts (with few positive results) as bargaining tactics in enterprise negotiations. It was the year employers in the manufacturing and metals sector saw off the unions’ ‘Campaign 2003’, giving little ground on the key issues of reduced hours and contributions to trust funds for worker entitlements. The year was a joyous one for employers in the building and construction industry, as their dreams of a shackled and weakened union movement came a step closer to being realised, with the introduction of draconian industry-specific legislation by the Howard Government, arising from the recommendations of the Cole Royal Commission. On a positive note, the year also witnessed all the members of the ‘industrial relations club’ embrace and declare a common concern for work and family balance issues.

Employer matters in 2004

n 2004, employers were active in arguing their cases in a number of important hearings of the Australian Industrial Relations Commission. However, despite a united position among employer ranks and the federal government, employers were generally disappointed with the Commission’s safety net review decision. Both the Australian industry Group and the Australian Chamber of Commerce and Industry found some common ground with the Australian Council of Trade Unions, in a consent position on extending carers leave, but overall employers presented a detailed argument opposing any extension of employee rights in the Commission’s work and family test case. Employers in some sectors were able to reach collective agreements with unions with little industrial disruption, whereas others, such as banking, found the going tougher. Overall, employers, like unions, faced a great deal of uncertainty over what were or were not ‘matters pertaining’, as a number of decisions after the Electrolux case clarified or clouded the issue. Understandably, the year ended on a positive note for most employers, with the Howard Government re-elected with a majority in the Senate, enabling it to pass a further round of radical labour market reforms in 2005.

Monitoring a commercial fermentation with proton nuclear magnetic resonance spectroscopy with the aid of chemometrics

Proton nuclear magnetic resonance spectroscopy (NMR) has shown the potential for being a valuable tool in monitoring a commercial fermentation. In this preliminary study, a suite of organic analytes including ethanol, fructose, glucose, methanol, glycerol, malic acid, tartaric acid, succinic acid, acetic acid and lactic acid were simultaneously determined during the fermentation. Data collection and analysis using chemometric algorithms aided the understanding of key processes including the effects of seeding a wine with bacteria for malo-lactic fermentation.

Employer matters in 2005

By any reckoning, the year 2005 will long be remembered as a watershed year for Australian industrial relations. While there were the usual types of industrial disputes, on-going enterprise bargaining and another round of arguments over the Australian Industrial Relations Commission’s (AIRC’s) annual safety-net review, the year was dominated by the looming re-writing of Australia’s industrial relations regulatory regime, made possible by the Government’s surprise majority in the Senate, granted to them in late 2004. Viewed as a looming dark cloud by some or a shining light by others, most of 2005 was spent in anxious anticipation of the Howard government’s impending ‘WorkChoices’ legislation. Employer groups spent much of the year lobbying the Howard government for the types of reforms long cherished, but only dreamed of by employers, for arguably 100 years. A once in a lifetime opportunity had presented itself and employer groups were determined to take full advantage of the situation, by ensuring that the government did not lose its nerve. Perhaps more importantly, however, in addition to lobbying the government, major employer organizations devoted significant resources to building the case for industrial relations reform and attempting to sell that message to the electorate. By year’s end, employers had succeeded in the first objective, but had seemingly failed in the second.

The environment always matters

Superficially, the high human populations of prosperous, well-fed city-states such as Singapore and Hong Kong might appear to support the view that high concentrations of human dynamism and enterprise can alone transcend environmental constraints. Indeed, extrapolations from such densely populated regions to the whole world form the historical basis for the most optimistic estimates of how many people the Earth might support. In fact, these affluent, densely populated regions require goods and services that are produced by areas far larger than the residential area of the state. For the foreseeable future, the whole world cannot live as densely and as well as Singapore. For example, some of the fish eaten in Hong Kong is captured in Pacific Island fisheries. Beef from the Northern Territory is exported to Japan. More generally, the supply of environmentally dependent goods - including sinks for wastes - is always limited, as are inputs such as fertile soil, clean fresh water and a benign climate.

Employer matters in 2006

If 2005 was a watershed year with the passing of the Work Choices legislation, then 2006 may well be considered year zero, symbolizing the beginning of a new era of Australian industrial relations under the employer friendly legislative regime. Employer groups were actively engaged in lobbying the Federal Government for further industrial relations reform, particularly in relation to the award rationalization process, and in pressuring the government for codification of the definition of `independent contractors', as a means of immunizing them from many of the rigours currently imposed by employment and labour law. Key employer groups made significant submissions to the newly formed Australian Fair Pay Commission in the lead up to its inaugural minimum wages decision, and though generally urging caution in raising minimum wages, there were nevertheless some differences of emphasis and approach apparent between a number of them. Despite an absence of widespread industrial disputation, the year witnessed a number of employers exercising their newfound powers — including some enhanced legal options — to either by-pass unions or to constrain union activity.

Perceptions of multiculturalism in music education: what matters and why

Pre-service teacher education students from two Australian universities were interviewed about their understandings of cultural diversity in music education. These initial findings revealed varied but generally consistent enthusiasm about including music from different cultures in teaching. However comments revealed an almost haphazard exposure to other musics. These were generally informal rather than learned in their formal education. Interviewees recognised the training that they had received in their tertiary studies in other cultures (both Western and non-Western) and expressed the intention to pursue professional development in their future careers. Engaging with the music of other cultures allows teachers and students to develop understanding and empathy with others. This is in line with current governmental initiatives on values that states that values education is intended to 'inspire and educate the next generation to see their world through the eyes of others. We want children to become adults who are caring, tolerant, fair and compassionate' (Department of Education, Science and Training, n.d., p. 2). Comments from the interviewees illustrate just such attitudes and understandings. It behoves us as educators to prepare students for teaching in multicultural classrooms that reflect the wider Australian society.

Optimal educational choice and redistribution when parental education matters

Higher education plays an important role in determining lifetime earnings. In turn, the decision to become educated depends to a large extent on family characteristics, such as wealth and education. In this paper, we focus on the interaction between fiscal policies and educational choices when parental education matters. We derive optimality conditions for a linear income tax and a lump‐sum subsidy for education in a dynamic framework in which generations are linked by educational background. The factors that determine their sign and magnitude include concerns for redistribution, efficiency, and the educational externality on future generations.