Apoptosis may underlie the pathology of zinc-deficient skin

The trace element zinc is essential for the survival and function of all cells. Zinc deficiency, whether nutritional or genetic, is fatal if left untreated. The effects of zinc deficiency are particularly obvious in the skin, seen as an erythematous rash, scaly plaques, and ulcers. Electron microscopy reveals degenerative changes within keratinocytes. Despite the well-documented association between zinc deficiency and skin pathology, it is not clear which cellular processes are most sensitive to zinc deficiency and could account for the typical pathological features. We used the cultured HaCaT keratinocyte line to obtain insight into the cellular effects of zinc deficiency, as these cells show many characteristics of normal skin keratinocytes. Zinc deficiency was induced by growing cells in the presence of the zinc chelator, TPEN, or by growth in zinc-deficient medium. Growth of cells in zinc-deficient medium resulted in a 44% reduction of intracellular zinc levels and a 75% reduction in the activity of the zinc-dependent enzyme, 5'-nucleotidase, relative to the control cells. Over a period of 7 days of exposure to zinc-deficient conditions, no changes in cell viability and growth, or in the cytoskeletal and cell adhesion systems, were found in HaCaT cells. At 7 days, however, induction of apoptosis was indicated by the presence of DNA fragmentation and expression of active caspase-3 in cells. These results demonstrate that apoptosis is the earliest detectable cellular change induced by zinc deficiency in HaCaT keratinocytes. Our observations account for many of the features of zinc deficiency, including the presence of degenerate nuclei, chromatin aggregates and abnormal organization of keratin, that may represent the later stages of apoptosis. In summary, a major causal role for apoptosis in the pathology of zinc deficiency in the skin is proposed. This role is consistent with the previously unexplained diverse range of degenerative cellular changes seen at the ultrastructural level in zinc-deficient keratinocytes.

The nationals may be heading into the wilderness, forever

Non-union rural workers may well be attracted to Kevin Rudd. IN RECENT decades, the Nationals have defied predictions of their demise. They have weathered the passing of agrarian socialism and the rapid decline in the farm population and - after the debacle of the "Joh for PM" campaign two decades ago - the party has successfully rallied around more limited goals of political survival and influence in the Coalition. Their parliamentary representation has fallen,...

Letters to a young Catholic, by George Weigel

With the Twentieth World Youth Day in Cologne on 16-21 August, this year, scheduled to be the first overseas visit of Pope Benedict XVI, it is timely to consider the latest book from George Weigel, (perhaps best known for his Witness to Hope: The Biography of John Paul II) in which he composes 14 letters specifically aimed at young Catholics, who, like many of their older counterparts, may well be wondering what it means to be a Catholic today.

Bondi Beach NSW, 28 May 2011, 11:06

This artwork is the result of the curators’ premise to invite an artist or artists to produce an initial image according to a brief. These initial images were then given to photographers to respond to and re-interpret the initial image. The curators produced a broadsheet publication and an online document cataloguing the works.

The demise of Bicycle George : a life of crime

This short piece looks at the life of a murderer, George Blunderfield (alias Arthur Oldring), who was hanged in Melbourne in 1918. Melburnians, or visitors to the city, may have seen his image on the wall at the Old Melbourne Gaol. Blunderfield's life started out normally
enough, and then descended into horrific crime. His story includes bicycle racing, escape from an island prison, and then recruitment for service with the Australian Imperial Forces in wartime Victoria. In the last years of his life, Blunderfield wreaked havoc from the western to the eastern coasts of Australia. This in turn had a dramatic effect on his immediate family, which is also detailed here. This story draws on the archives at PROV as well as on State Records Office of Western Australia material, with help from Ms Jean
Bellamy, a distant relative of George Blunderfield.