3 resultados para Laboratory plasma

em Deakin Research Online - Australia


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A new plasma laboratory has been established in the Institute for Frontier Materials (IFM) at Deakin University, Geelong. The two major research themes are (1) tailoring of surfaces/interfaces with new functionality, and (2) fabrication/doping of nanomaterials. The aim is to meet the challenge of better performing materials in applications ranging from energy, biomedicine and nanotechnology to composites, transport and textiles. Plasma technology offers an alternative to conventional approaches and its success depends on an improved understanding of the underlying mechanisms. We promote a team spirit in which different experts harmoniously work together. More than thirty PhD studies and collaborative projects have been undertaken and proposed since 2009 - within IFM, across the University and with outside research organisations nationally and internationally.

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During his PhD studies, Zhiqiang published 5 journal paper and 7 conference paper and also participated in the development of the first Plasma Research Laboratory at Institute of Frontier Materials.

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OBJECTIVES: In widely used protocols for the collection and isolation of cord blood mononuclear cells, investigators are left with substantial volumes of diluted plasma which could be used for other measurements. The aim of this study was to ascertain the validity of umbilical cord blood (UCB) diluted plasma samples for vitamin D, A and E analysis compared to UCB serum samples. DESIGN & METHODS: Twenty UCB matched samples of diluted plasma and serum were collected. The samples were analysed by two liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods on two separate occasions. RESULTS: The results of 25(OH)D3 obtained by the two laboratories demonstrated close agreement with a mean difference of 0.14nmol/L [95% confidence interval (95% CI), -6.8 to 7.1]. Both methods demonstrate close agreement for 25(OH)D3 in UCB serum versus diluted UCB plasma; mean difference 2.2nmol/L [95% CI, -9.5 to 13.9] and 4.1nmol/L [95% CI, -14.5 to 6.1] for the results from Lab A and Lab B, respectively. Vitamin A was quantified by Lab A in UCB serum and diluted UCB plasma; mean difference 0.07μmol/L [95% CI, -0.41 to 0.28]. Results of 25(OH)D3 epimer and vitamin E in the diluted UCB plasma were below the limit of quantification, and could not be compared with UCB serum. CONCLUSIONS: Diluted UCB plasma can be used for the quantification of retinol and 25(OH)D3 by LC-MS/MS. By contrast, quantification of 25(OH)D3 epimer and vitamin E in diluted UCB plasma is not supported by this study due to limitations in analytical sensitivity.