A low-cost 3-D laser imaging system

This paper presents the design and development of a low cost three-dimensional laser imaging system for scanning suitable surfaces. A generic, low cost, off-the-shelf laser range finder is used to obtain the primary one dimensional distance measurement. The range finder’s laser beam is reflected by a twin-axis mirror assembly driven by stepper motors providing the system with two angular degrees of freedom, allowing 3-D measurements to be determined. A camera and image processing techniques are used to determine the measured 1-D range value from the generic range-finding device. A computer program then uses the obtained data to create a 3-D point cloud. An algorithm is then used to construct a 3-D wire frame mesh representing the scanned surface. The system has an angular resolution of 1.8° and the results obtained demonstrate the system to have an accuracy of approximately ± 2cm at a scanning distance of 1.0m.

Seafloor mapping data, Victoria

The collection includes three major data types

- Seafloor structure information: Collation of seafloor structure information (bathymetry and softness hardness) collected using multibeam sonar systems as part of the Victorian Marine Habitat Mapping Project and bathymetric light detection and ranging data (LiDAR) collected as part of the future coats program. The geographic is Victorian State waters (~1400km2 multibeam sonar & ~4000 km2 for LiDAR as of 2012

- Video information: ~800 linear kilometres of precisely georeferenced towed video data from depths of 10-100m in Victorian coastal waters. 300 baited stereo video drops in Hopkins and Discovery Bay site locations. All video has been classified to the lowest taxonomic resolution possible with the video systems employed and converted to spatial data layers in the GIS environment.

- Habitat maps: Predicted habitat models integrating seafloor structure and video information developed using classification algorithms and remote sensing technologies.

Confocal laser scanning microscopy elucidation of the micromorphology of the leaf cuticle and analysis of its chemical composition

Electron microscopy techniques such as transmission electron microscopy (TEM) and scanning electron microscopy (SEM) have been invaluable tools for the study of the micromorphology of plant cuticles. However, for electron microscopy, the preparation techniques required may invariably introduce artefacts in cuticle preservation. Further, there are a limited number of methods available for quantifying the image data obtained through electron microscopy. Therefore, in this study, optical microscopy techniques were coupled with staining procedures and, along with SEM were used to qualitatively and quantitatively assess the ultrastructure of plant leaf cuticles. Leaf cryosections of Triticum aestivum (wheat), Zea mays (maize), and Lupinus angustifolius (lupin) were stained with either fat-soluble azo stain Sudan IV or fluorescent, diarylmethane Auramine O and were observed under confocal laser scanning microscope (CLSM). For all the plant species tested, the cuticle on the leaf surfaces could be clearly resolved in many cases into cuticular proper (CP), external cuticular layer (ECL), and internal cuticular layer (ICL). Novel image data analysis procedures for quantifying the epicuticular wax micromorphology were developed, and epicuticular waxes of L. angustifolius were described here for the first time. Together, application of a multifaceted approach involving the use of a range of techniques to study the plant cuticle has led to a better understanding of cuticular structure and provides new insights into leaf surface architecture.