Automatic age estimation based on facial aging patterns

While recognition of most facial variations, such as identity, expression, and gender, has been extensively studied, automatic age estimation has rarely been explored. In contrast to other facial variations, aging variation presents several unique characteristics which make age estimation a challenging task. This paper proposes an automatic age estimation method named AGES (AGing pattErn Subspace). The basic idea is to model the aging pattern, which is defined as the sequence of a particular individual's face images sorted in time order, by constructing a representative subspace. The proper aging pattern for a previously unseen face image is determined by the projection in the subspace that can reconstruct the face image with minimum reconstruction error, while the position of the face image in that aging pattern will then indicate its age. In the experiments, AGES and its variants are compared with the limited existing age estimation methods (WAS and AAS) and some well-established classification methods (kNN, BP, C4.5, and SVM). Moreover, a comparison with human perception ability on age is conducted. It is interesting to note that the performance of AGES is not only significantly better than that of all the other algorithms, but also comparable to that of the human observers.

Whose culture, where and why? – Identity, culture and artistic expression

This research addresses how the visual and artistic expression represents my cultures and gendered identities. My journey mapped cultural inheritances, ancestry and postcolonial influences from home country, my childhood memories, life experiences and migration transitions to Australia. A series of my artworks represent cultural markers, a sense of belonging, nostalgia and challenges of relocation. Findings support how art expression can forge ‘affective links’ and develop pedagogies for intercultural understanding through Art Education.

Steroid 21-hydroxylase expression in cultured rat astrocytes

Over the past decade or so it has become widely recognised that the brain is a significant steroidogenic organ. Many publications have highlighted the ability of the brain to synthesise and interconvert a large number of steroid products including cholesterol, progesterone and testosterone. In this study, in vitro experiments were performed to determine if 21-hydroxylation of steroids is undertaken by rat brain astrocytes in culture. This is a common reaction that occurs in the adrenal gland and other organs in mammals, catalysing the conversion of pregn-4-ene-3,20-dione (progesterone) to 21-hydroxypregn-4-ene-3,20-dione (deoxycorticosterone).

Previous reports have indicated that 21-hydroxylation occurs within the rat brain, however, the precise identity of the cells expressing 21-hydroxylase has not yet been determined. Several metabolites, such as 5α-pregnan-3α-ol-20-one (tetrahydroprogesterone) and 3α,21-dihydroxy-5-pregnan-20-one (tetrahydrodeoxycorticosterone) were of particular interest because of their modulatory role in neuronal function, such as their agonist activity at γ-aminobutyric acid (GABA_A) receptors.

Evidence was obtained for the expression of peripheral 21-hydroxylase enzyme (P450c21) in cultured rat brain astrocytes by a combination of mass spectroscopy and molecular biology techniques. This is a significant finding as expression of 21-hydroxylase within astrocytes may be indicative of a wider role for these cells in modulating neuronal behaviour.

Hybrid perspectives : defining the relativity of culture and identity through migration experiences

This paper examines how culture and identity can be relatively defined through hybrid perspectives in relation to migration experiences. Addressing and portraying definitions of culture and identity is crucial in understanding how notions of such issues connect and initiate the migrant subject through new experiences, perspectives and ways of being. In enunciating the transitions from home to a new place, and elaborating on the rupture of an inherited culture and grounded identity, I refer to them through self-reflexive perspectives. The search for meaning through appraisals of cultural lineage and linguistic capital through a Diaspora, a post colonial history and lived life experiences from my home country, pre-empts the ambivalent and hybrid status in defining culture(s) and identit(ies). It is crucial to recognise how challenges for adaptation to new culture, language, societal norms, and differences in class, nationality, race and gender play specific roles in the migrant experience. My current experiences of migration to Australia are narrations of encountered difficulties, fears, inhibitions, new aspirations, perceptions and perspectives, which map an ‘identity crisis.’ From this narrative structure, I investigate through my ongoing PhD study, how my artistic expression and representations progress towards experiences, and themes that metaphorically reflect, inspire and enact the hybrid structures of culture(s) and identit(ies). Explored reflexively my representations suggest how the ‘liminal space’ or the ‘third space,’ (Bhabha, 1990) express transitions about the ‘self’ and my artistic expression, which enable further reflection and positions to emerge and extend to metaphorical expressions.

Expression of muscarinic receptor subtypes in tree shrew ocular tissues and their regulation during the development of myopia

Muscarinic receptors are known to regulate several important physiologic processes in the eye. Antagonists to these receptors such as atropine and pirenzepine are effective at stopping the excessive ocular growth that results in myopia. However, their site of action is unknown. This study details ocular muscarinic subtype expression within a well documented model of eye growth and investigates their expression during early stages of myopia induction. Total RNA was isolated from tree shrew corneal, iris/ciliary body, retinal, choroidal, and scleral tissue samples and was reverse transcribed. Using tree shrew-specific primers to the five muscarinic acetylcholine receptor subtypes (CHRM1-CHRM5), products were amplified using polymerase chain reaction (PCR) and their identity confirmed using automated sequencing. The expression of the receptor proteins (M1-M5) were also explored in the retina, choroid, and sclera using immunohistochemistry. Myopia was induced in the tree shrew for one or five days using monocular deprivation of pattern vision, and the expression of the receptor subtypes was assessed in the retina, choroid, and sclera using real-time PCR. All five muscarinic receptor subtypes were expressed in the iris/ciliary body, retina, choroid, and sclera while gene products corresponding to CHRM1, CHRM3, CHRM4, and CHRM5 were present in the corneal samples. The gene expression data were confirmed by immunohistochemistry with the M1-M5 proteins detected in the retina, choroid, and sclera. After one or five days of myopia development, muscarinic receptor gene expression remained unaltered in the retinal, choroidal, and scleral tissue samples. This study provides a comprehensive profile of muscarinic receptor gene and protein expression in tree shrew ocular tissues with all receptor subtypes found in tissues implicated in the control of eye growth. Despite the efficacy of muscarinic antagonists at inhibiting myopia development, the genes of the muscarinic receptor subtypes are neither regulated early in myopia (before measurable axial elongation) nor after significant structural change.

Greek as a marker of identity in Melbourne, Australia

Individual and group identity is often closely associated with language use. Language, in turn, often serves as a proxy for culture which provides the background against which language use occurs. For the Greek community in Melbourne, Australia, use of Greek is an important symbolic aspect of ethnic identification and personal and groupidentity. Even for those younger members of the community whose daily interactions occur primarily in English and who view themselves as first language speakers of English, Greek plays a specific role in expression of personal identity and cultural expression. The use of Greek provides a link to the culture of origin and serves as a symbolic marker of association with a specific group in the larger Australian context.For first generation Greek Australians, exposure to the language and culture came primarily from immigrant parents. However, many of these individuals also attended Greek school which served to reinforce their knowledge and ability to use the language. Their children, the second generation, often use Greek words routinely in specific contexts, such as when talking about food and religion or when referring to family members (grandmother, grandfather). While they often attend Greek school as well, there is evidence that overall ability to speak Greek fluently in the community is declining. Nonetheless, selective use of Greek terms remains an important identity marker. This paper will describe the use of Greek words and terms by English–speaking members of the Melbourne community and discuss its significance as a form of cultural identification and personal identity. The phenomenon of Greek school as a vehicle for language exposure will also be discussed. Data, based on in depth interviews with members of the Greek community, will be used to illustrate the contexts in which switches to Greek occur and elucidate the cognitive background of such usage.

Characterisation and vascular expression of nitric oxide synthase 3 in amphibians

In mammals, nitric oxide (NO) produced by nitric oxide synthase 3 (NOS3) localised in vascular endothelial cells is an important vasodilator but the presence of NOS3 in the endothelium of amphibians has been concluded to be absent, based on physiological studies. In this study, a nos3 cDNA was sequenced from the toad, Rhinella marina. The open reading frame of R. marina nos3 encoded an 1170 amino acid protein that showed 81 % sequence identity to the recently cloned Xenopus tropicalis nos3. Rhinella marina nos3 mRNA was expressed in a range of tissues and in the dorsal aorta and pulmonary, mesenteric, iliac and gastrocnemius arteries. Furthermore, nos3 mRNA was expressed in the aorta of Xenopus laevis and X. tropicalis. Quantitative real-time PCR showed that removal of the endothelium of the lateral aorta of R. marina significantly reduced the expression of nos3 mRNA compared to control aorta with the endothelium intact. However, in situ hybridisation was not able to detect any nos3 mRNA in the dorsal aorta of R. marina. Immunohistochemistry using a homologous R. marina NOS3 antibody showed immunoreactivity (IR) within the basal region of many endothelial cells of the dorsal aorta and iliac artery. NOS3-IR was also observed in the proximal tubules and collecting ducts of the kidney but not within the capillaries of the glomeruli. This is the first study to demonstrate that vascular endothelial cells of an amphibian express NOS3.