Expression and regulation of Homer in human skeletal muscle during neuromuscular junction adaptation to disuse and exercise

Protein calcium sensors of the Homer family have been proposed to modulate the activity of various ion channels and nuclear factor of activated T cells (NFAT), the transcription factor modulating skeletal muscle differentiation. We monitored Homer expression and subcellular localization in human skeletal muscle biopsies following 60 d of bedrest [Second Berlin Bedrest Study (BBR2-2)]. Soleus (SOL) and vastus lateralis (VL) biopsies were taken at start (pre) and at end (end) of bedrest from healthy male volunteers of a control group without exercise (CTR; n=9), a resistive-only exercise group (RE; n=7), and a combined resistive/vibration exercise group (RVE; n=7). Confocal analysis showed Homer immunoreactivity at the postsynaptic microdomain of the neuromuscular junction (NMJ) at bedrest start. After bedrest, Homer immunoreactivity decreased (CTR), remained unchanged (RE), or increased (RVE) at the NMJ. Homer2 mRNA and protein were differently regulated in a muscle-specific way. Activated NFATc1 translocates from cytoplasm to nucleus; increased amounts of NFATc1-immunopositive slow-type myonuclei were found in RVE myofibers of both muscles. Pulldown assays identified NFATc1 and Homer as molecular partners in skeletal muscle. A direct motor nerve control of Homer2 was confirmed in rat NMJs by in vivo denervation. Homer2 is localized at the NMJ and is part of the calcineurin-NFATc1 signaling pathway. RVE has additional benefit over RE as countermeasure preventing disuse-induced neuromuscular maladaptation during bedrest.

Who is the 'lovable larikan'? : an historical inquiry using biography and autobiography

Every profession has its myth that defines its self-identity and work culture. For nursing, it's Florence Nightingale; for theatre, Homer and Shakespeare; for medicine, Hippocrates. Australian journalism too, has its myth - that of the hard-working, hard-drinking, aggressive and defiant 'Lovable Larrikin'. But unlike other professions, Australian journalism's 'myth' cannot be pinned down to one historical figure. It is therefore difficult to investigate the 'real' story behind the myth. Using an open-coding analysis of biographical and autobiographical material, this paper aims to detect larrikin-like characteristics among early Australian journalists (Colonial era to, and including, the interwar period), to identify significant people and events that developed larrikinism as a specific Australian journalism identity.

The mundiad : sic transit gloria mundi

The Mundiad is a mock epic poem in heroic couplets. Modelled on structures of classical epics such as Homer's Iliad, Virgil's Aeneid and Milton's Paradise Lost, yet set in the contemporary world of globalisation, the poem celebrates the detritus of everyday life Kylie Minogue, pornography, new ageism, genetic engineering, IVF, screen culture, among many others. Reviving the ancient poetic ambition to speak differently about the things of this world, The Mundiad is startlingly original and is destined to be a cult classic.

Joyce and the ‘Cultic Twalette’: Jocoserious jousts in the nationalist arena in the Cyclops episode of Ulysses

Set in a richly detailed Dublin of 1904, there has never been serious debate about the Irishness of Ulysses, but early critics and commentators, abetted by Joyce, made more of its European intertexts especially Homer, Dante, and closer to Ireland, Shakespeare. Its deep and suppressed debt to the Irish tradition was not fully explored until Maria Tymoczko’s The Irish Ulysses (1994). This paper examines how ancient Irish poetic material is used and strategically coopted for political use in the so-called Cyclops chapter of Ulysses, and demonstrates how Joyce reworked ancient Irish poetry to express a rather more inclusive and celebratory relationship with his cultural heritage than is sometimes recognised by commentators who more commonly insist that his use of ancient Irish material is parodic and satiric.

Speaking as 'other'

As a child of Chilean migrants I have always felt a strong affinity with the displaced and vulnerable. It would be as difficult to change this as it would be to change my place of birth for this is my inheritance. By this i mean the physics of who I am - woman, non- white, Chilean born; the social milieu and economic conditions that I was born into - Catholic and working class; and the politics that i have adopted - a commitment to social and political reform.

Prospect of renewable energy sources and integrating challenges in Victoria, Australia

The Australian Government has targeted 20% of electricity generation from renewable energy sources by 2020. The 20% renewable energy target (RET) for Australia will be around 45000GWH in 2020. Renewable energy of Victoria also includes as part of support to achieve 20% RET in Australia. In Victoria wind and solar resources are abundant as compared to other sources of renewable energy. Solar radiation and wind speed data for Victoria State was collected from NASA surface meteorology and solar energy web site. A feasibility analysis has been carried out to explore the potentialities of wind and solar energy for Victoria State using hybrid optimization model of electric renewable (HOMER) software. This paper also represents the estimation analysis of RET for Victoria and describes the integrating challenges of renewable energy sources to the utility grid.

Potentialities of renewable energy in Victoria, Australia

In utility power system, electricity demand is being covered largely by fossil fueled power generation, which contributes high level of GHG (greenhouse gas) emission and causes global warming worldwide. In order to reduce GHG emission level, most of the countries in the world targeting towards green energy that is power generation from RE (renewable energy) sources. In this paper, it is considered to study prospects of RE sources in particular, solar and wind in Victoria State which are abundant as compared to other sources of renewable. The wind and solar energy feasibility study and sensitivity analysis has been done for Victoria with the aid of HOMER (hybrid optimization model of electric renewable) simulation software. From the study, it has clearly evicted that wind energy combinational HPS (hybrid power system) has more contribution, and high potential than solar PV (photovoltaic) systems for a particular location. This study also investigates the influences of energy storage in the proposed HPS.

Performance study of a wind-grid system in a semi- arid region and major integration issues

The phenomenal growth in economy experienced in developed countries throughout the 20th century has largely been driven by the availability of conventional energy sources for electricity generation. However, increased concern about fossil fuels and adverse effect of carbon dioxide emission in to atmosphere changed the conventional power system to a viable one by integrating renewable energy sources into the existing system. Among the Renewable Energy (RE) sources, wind energy is one of the fastest growing technologies in reducing the Green House Gas (GHG) emissions in to the atmosphere due to its continuous availability throughout a period. Hence, this paper discusses the performance of a wind-grid connected system in a semi-arid region by conducting a case study. Wilson promontory, one of the best locations for wind generation in Victoria is considered as a case study. Hybrid Optimization Model for Electric Renewable (HOMER) is used as a simulating tool for this analysis. This study also presents the influences of storage system in the proposed Hybrid Power System (HPS) allowing energy to be stored during higher generations or lower load demands. In addition this paper also discusses the major integration issues to facilitate the large scale wind energy into the grid for reliable power generation and distribution.

Prospects of renewable energy in semi-arid region

Continuous usage of fossil fuels and other conventional resources to meet the growing demand has resulted in in-creased energy crisis and greenhouse gas emissions. Hence, it is essential to use renewable energy sources for more reliable, effective, sustainable and pollution free transmission and distribution networks. Therefore, to facilitate large-scale integration of renewable energy in particular wind and solar photovoltaic (PV) energy, this paper presents the feasibility analysis for semi-arid climate and finds the most suitable places in North East region of Victoria for re-newable energy generation. For economic and environmental analysis, Hybrid Optimization Model for Electric Re-newables (HOMER) has used to investigate the prospects of wind and solar energy considering the Net Present Cost (NPC), Cost of Energy (COE) and Renewable fraction (RF). Six locations are selected from North East region of Victo-ria and simulations are performed. From the feasibility analysis, it can be concluded that Mount Hotham is one of the most suitable locations for wind energy generation while Wangaratta is the most suitable location for solar energy generation. Mount Hotham is also the best suitable locations in North East region for hybrid power systems i.e., com-bination of both wind and solar energy generation.

Paediatric leukaemia DNA methylation profiling using MBD enrichment and SOLiD sequencing on archival bone marrow smears

BACKGROUND: Acute Lymphoblastic Leukaemia (ALL) is the most common cancer in children. Over the past four decades, research has advanced the treatment of this cancer from a less than 60% chance of survival to over 85% today. The causal molecular mechanisms remain unclear. Here, we performed sequencing-based genomic DNA methylation profiling of eight paediatric ALL patients using archived bone marrow smear microscope slides. FINDINGS: SOLiD™ sequencing data was collected from Methyl-Binding Domain (MBD) enriched fractions of genomic DNA. The primary tumour and remission bone marrow sample was analysed from eight patients. Four patients relapsed and the relapsed tumour was analysed. Input and MBD-enriched DNA from each sample was sequenced, aligned to the hg19 reference genome and analysed for enrichment peaks using MACS (Model-based Analysis for ChIP-Seq) and HOMER (Hypergeometric Optimization of Motif EnRichment). In total, 3.67 gigabases (Gb) were sequenced, 2.74 Gb were aligned to the reference genome (average 74.66% alignment efficiency). This dataset enables the interrogation of differential DNA methylation associated with paediatric ALL. Preliminary results reveal concordant regions of enrichment indicative of a DNA methylation signature. CONCLUSION: Our dataset represents one of the first SOLiD™MBD-Seq studies performed on paediatric ALL and is the first to utilise archival bone marrow smears. Differential DNA methylation between cancer and equivalent disease-free tissue can be identified and correlated with existing and published genomic studies. Given the rarity of paediatric haematopoietic malignancies, relative to adult counterparts, our demonstration of the utility of archived bone marrow smear samples to high-throughput methylation sequencing approaches offers tremendous potential to explore the role of DNA methylation in the aetiology of cancer.