Synthesis of ethyl acetate employing celite-immobilized lipase of Bacillus cereus MTCC 8372

A wide range of fatty acid esters can be synthesized by esterification and transesterification reactions catalyzed by lipases in non-aqueous systems. In the present study, immobilization of a purified alkaline extra-cellular lipase of Bacillus cereus MTCC 8372 by adsorption on diatomaceous earth (celite) for synthesis of ethyl acetate via transesterification route was investigated. B. cereus lipase was deposited on celite (77% protein binding efficiency) by direct binding from aqueous solution. Immobilized lipase was used to synthesis of ethyl acetate from vinyl acetate and ethanol in n -nonane. Various reaction conditions, such as biocatalyst concentration, substrates concentration, choices of solvents ( n -alkanes), incubation time, temperature, molecular sieves (3Å × 1.5 mm), and water activity(a _w ), were optimized. The immobilized lipase (25 mg/ml) was used to perform transesterification in n -alkane(s) that resulted in approximately 73.7 mM of ethyl acetate at 55 °C in n -nonane under shaking (160 rpm) after 15 h, when vinyl acetate and ethanol were used in a equimolar ratio (100 mM each). Addition of molecular sieves (3Å × 1.5 mm) as well as effect of water activity of saturated salt solutions (KI, KCl and KNO ₃ ) to the transesterification efficiency has inhibitory effect. Batch operational stability tests indicated that immobilized lipase had retained 50% of its original catalytic activity after four consecutive batches of 15 h each.

Fault detection for dynamical systems using first-order functional observers

This paper reports a new result on the fault detection of dynamical systems by employing only first-order functional observers. Indeed, we show that fault detection can be achieved by utilizing first-order functional observers. The advantages for having such simple structured observers are obvious from the economical and practical points of view as significant cost saving can be achieved. We derive existence conditions and an algorithm for the generation of residual signals to detect faults using firstorder functional observers. Two numerical examples are given to illustrate the proposed fault detection scheme. In one of the examples, a two-area interconnected power system with reheat thermal turbines is considered where only a first-order functional observer is designed to detect faults in the power system.

An enhanced stability criterion for time-delay systems via a new bounding technique

In the past few years, a great deal of effort has been devoted to improve delay-dependent conditions for stability of linear systems with an interval time-varying delay. To reduce conservatism of stability conditions, in the framework of the Lyapunov-Krasovskii functional (LKF) method, the bounding technique plays a key role. In this paper, a new bounding technique based on a new integral inequality is proposed. By employing the newly bounding technique proposed in this paper, an enhanced stability criterion for a class of linear systems with an interval time-varying delay is derived. The effectiveness and a significant improvement of the obtained results are shown by numerical examples.

An improved stability criterion for time-delay power systems with electric vehicles and high voltage direct current power links

This paper presents an improved stability criterion for load frequency control (LFC) of time-delay power systems including AC/HVDC transmission links and EVs. By employing a novel refined Jensen-based inequality, an improved stability condition is derived in terms of feasible linear matrix inequalities (LMIs) which allow us to compute the maximal upper bounds of time-delay ensuring stability of the LFC scheme equipped with an embedded controller. Cases studies here are implemented for LFC scheme of a two-area power system, which is interconnected by parallel (AC/HVDC) links, with embedded proportional integral (PI) controller for discharged EVs. The relationships between the parameters of PI controller, supplementary control of HVDC links and delay margins of the LFC scheme are also discussed. As a consequence of facts, the results of delay margins can be used as a guideline to tune PI controller and set-up parameters for HVDC control.