Investigation of compounds causing water repellency in the rhizosphere of sandy soils from a wide range of locations.

Although soils are generally considered to wet readily, some are actually water repellent at the surface and in the rhizosphere. This phenomenon occurs at low to moderate moisture contents and has been reported from soils under a range of vegetation types and from many regions around the globe. Water repellency in soils can have serious environmental implications including reduced seed germination and plant growth as well as irrigation efficiency, accelerated soil erosion, and enhanced leaching of agrochemicals through preferential flow. it has been proposed that water repellency is caused by the accumulation of hydrophobic organic compounds released as root exudates, microbial byproducts or from decomposing organic matter, which are deposited on mineral or aggregate surfaces, or are present as interstitial matter, Few studies to date have attempted to isolate and characterize these compounds and their structure is therefore only poorly understood, These studies have generally focussed on only a single soil or a small range of samples, have not included non-repellent soils as a control and have not always been able to demonstrate that the substances isolated are indeed responsible for repellency formation.

This study reports on the first part (extraction procedures) of a research programme addressing these gaps in current knowledge by investigating a wide range of severely repellent and wettable ‘control’ samples from different countries, and by including assessments of extraction efficiency and ability of extracts to cause repellency. Analytical methods include DRIFT (Diffuse Reflectance Infrared Fourier Transform Spectroscopy) of soils and IR (Infrared) analysis of extracts.

Key findings are that (i) soil sample heating after extraction is valuable in assessing the effectiveness of the extraction procedure, (ii) Soxhlet extraction using isopropanol/ ammonia (70/30 v/v) was the most effective method in extracting hydrophobic compounds, while leaving the ability of extracted compounds to induce water repellency virtually unaffected, (iii) wettable control soils also contain hydrophobic substances capable of inducing water repellency, (iv) the amount of organic compounds extracted was poorly related to sample repellency, indicating that compounds responsible for repellency may only represent a small fraction of the extract, (v) differences in extraction efficiency between different samples indicate that the compounds responsible may differ generically and/or in terms of their bonding to minerals, and (vi) the combination of repellency assessments with DRIFT on soils and JR on extracts used with internal standards has considerable potential to allow quantification of CH bearing organic matter in the soil, the efficiency of extraction processes for its removal, and its significance in causing water repellency in soils.

Effects of fibre parameters on the ultraviolet protection of fibre assemblies

Ultraviolet (UV) radiation protection is becoming increasingly necessary for human health, and textiles play an important role. The interaction between UV light and textiles is a complex one, involving fibre, yarn and fabric parameters. In this study, an optical model is presented for examining the influences of fibre parameters on the UV protection offered by a bundle of fibres with a given mass. The effects of mean fibre diameter and fibre type on UV absorption were examined. The model was verified with results of UV–visible diffuse reflectance measurements on natural and synthetic fibres. When the mass of fibres was kept constant, within the measurement range in this study, a bundle of fibres with coarser fibres had a lower UV reflectance than that with finer ones. The model accurately predicted factors influencing UV protection, including fibre diameter, fibre transmittance, porosity and refractive index.

UV protection performance of textiles affected by fiber cross-sectional shape

Abstract
In this study, a model was set up for simulating the effects of varying fiber cross-sectional shapes on ultraviolet protection of fibers. The fiber diameter and fiber type were also involved in the model setting. Experiments of diffuse reflectance spectra measurement on natural (wool, cotton, silk), regenerated (bamboo viscose) and synthetic (polyester, nylon) fibers were conducted to verify the model predicted results. When a more complex shape was assumed as the fiber cross-section for model calculation, the predicted results have a better agreement with the actual results. The effects on ultraviolet absorption from fibers with different cross-sectional shapes were investigated at a single fiber, fiber bundle and yarn levels. With the same material, when the fiber cross-sectional area and the areal coverage of a single fiber were constant, the triangular shape had the lowest ultraviolet transmittance and the highest ultraviolet reflectance for a single fiber and also for a fiber bundle. The difference of fiber cross-sectional shapes was also significant in the ultraviolet protection of a single yarn.

Research on the influence of yarn parameters on the ultraviolet protection of yarns

Considering both the yarn parameters and the light interaction (reflectance and transmittance) between two adjacent yarns, an optical model was presented to understand the ultraviolet (UV) light penetrating a single undyed yarn and a lot of yarns. The optical model was verified with results of diffuse reflectance spectra measurement on wool yarn samples. This optical model was used to predict the factors influencing UV protection, including fibre diameter, yarn linear density, yarn twist, transmittance index and refractive index. The statistical predictive model was also set up to show the relationship between the yarn parameters and the UV protection (UPF values) of the yarns. Yarns with the fine diameter, large yarn linear density and low yarn twist had the high UV protection.

Modelling the diffuse fraction of global solar radiation on a horizontal surface

For various applications it is necessary to know not only global solar radiation values, but also the diffuse and beam components. Because often only global values are available, there have been several models developed to establish correlations between the diffuse fraction and various predictors. These typically include the clearness index, but also may include the solar angle, temperature and humidity. The clearness index is the proportion of extraterrestrial radiation reaching a location, where the extraterrestrial value used in the calculation varies with latitude and time of year. These correlations have been developed using data principally from latitudes greater than 40°, often using only data from a few locations and with few exceptions have not used solar altitude as a predictor. Generally the data consist of hourly integrated values. A model has been developed using hourly data from a weather station set up at Deakin University, Geelong. Another model has also been developed for 15 minute data values in order to ascertain if the smoothing generated by using hourly data makes a significant difference to overall results. The construction of such models has been investigated, enabling an extension to the research, inclusive of other stations, to be performed systematically. A final investigation was carried out, using data from other Australian locations, to explain some of the considerable scatter by adding apparent solar time as a predictor, which proved to be significantly better than solar altitude.

Egg colour matching in an African cuckoo, as revealed by ultraviolet-visible reflectance spectrophotometry

Despite major differences between human and avian colour vision, previous studies of cuckoo egg mimicry have used human colour vision (or standards based thereon) to assess colour matching. Using ultraviolet-visible reflectance spectrophotometry (300-700 nm), we measured museum collections of eggs of the red-chested cuckoo and its hosts. The first three principal components explained more than 99% of the variance in spectra, and measures of cuckoo-host egg similarity derived from these transformations were compared with measures of cuckoo-host egg similarity estimated by human observers unaware of the hypotheses we were testing. Monte Carlo methods were used to simulate laying of cuckoo eggs at random in nests. Results showed that host and cuckoo eggs were very highly matched for an ultraviolet versus greenness component, which was not detected by humans. Furthermore, whereas cuckoo and host were dissimilar in achromatic brightness, humans did not detect this difference. Our study thus reveals aspects of cuckoo-host egg colour matching which have hitherto not been described. These results suggest subtleties and complexities in the evolution of host-cuckoo egg mimicry that were not previously suspected. Our results also have the potential to explain the longstanding paradox that some host species accept cuckoo eggs that are non-mimetic to the human eye.

Plumage reflectance and the objective assessment of avian sexual dichromatism

Assessment of color using human vision (or standards based thereon) is central to tests of many evolutionary hypotheses. Yet fundamental differences in color Vision between humans and other animals call this approach into question. Here we use techniques for objectively assessing color patterns that avoid reliance on species-specific (e.g., human) perception. Reflectance spectra are the invariant features that we expect the animal's color cognition to have evolved to extract. We performed multivariate analyses on principal components derived from >2,600 reflectance spectra (300-720 nm) sampled in a stratified random design from different body regions of male and female starlings in breeding plumage. Starlings possess spatially complex plumage patterns and extensive areas of iridescence. Our study revealed previously unnoticed sex differences in plumage coloration and the nature of iridescent and noniridescent sex differences. Sex differences occurred in some body regions bur not others, were more pronounced at some wavelengths (both ultraviolet and human visible), and involved differences in mean reflectance and spectral shape. Discriminant analysis based on principal components were sufficient to sex correctly 100% of our sample. If hidden sexual dichromatism is widespread, then it has important implications for classifications of animals as mono- or dimorphic and for taxonomic and conservation purposes.

The role of ultraviolet-A reflectance and ultraviolet-A-induced fluorescence in budgerigar mate choice

Many parrots have plumage that either reflects strongly in the ultraviolet-A (UVA) waveband, between 315-400 nm, or exhibits UVA-induced fluorescence. Previous experimental work on budgerigars (Melopsittacus undulatus) suggests that UVA reflectance plays a role in mate choice, as in other diurnal birds, but evidence for fluorescent cues playing a role is unconvincing. Here we report two experiments on budgerigars, designed to determine whether fluorescent cues play a role in signalling when UVA reflectances are absent, an approach which separates removal of UVA reflectance from removal of fluorescence. First, we determined whether the choices of different females are correlated under these treatment conditions. Secondly, we investigated female preferences for fluorescing and non-fluorescing males when UVA reflections are absent, to determine whether the yellow emissions of fluorescence are playing a role in mate choice. Results from experiment 1 do not suggest that females agree on which males are attractive when UVA reflectances are absent, with only half of the subjects choosing the same male. Neither did different females make the same choices in experiment 2. This lack of agreement provides further evidence that UVA reflectances from males play an important role in female choice in this species. Experiment 2 provides no evidence to suggest that UVA-induced fluorescence plays a role in mate choice. Overall, our study supports previous findings showing that UVA reflectance plays a role in sexual signalling in this species, but provides no evidence to suggest the same for fluorescence when UVA reflectances are absent.

The role of ultraviolet-A reflectance and ultraviolet-A induced fluorescence in the appearance of budgerigar plumage: insights from spectrofluorometry and reflectance spectrophotometry

Fluorescence has so far been found in 52 parrot species when illuminated with ultraviolet-A (UVA) 'black' lamps, and two attempts have been made to determine whether such fluorescence plays any role in sexual signalling. However, the contribution of the reflectance versus fluorescence to the total radiance from feathers, even in the most studied species to date (budgerigars), is unclear. Nor has the plumage of this study species been systematically assessed to determine the distribution of fluorescent patches. We therefore used spectrofluorometry to determine which areas of budgerigars fluoresce and the excitation and emission spectra involved; this is the first time that such a technique has been applied to avian plumage. We found that both the yellow crown and (normally hidden) white downy chest feathers exhibit strong UVA-induced fluorescence, with peak emissions at 527 nm and 436 nm, respectively. Conversely, the bright-green chest and dark-blue tail feathers do not fluoresce. When comparing reflectance spectra (400700 nm) from the yellow crown using illuminants with a proportion of UVA comparable to daylight, and illuminants with all UVA removed, no measurable difference resulting from fluorescence was found. This suggests that under normal daylight the contribution of fluorescence to radiance is probably trivial. Furthermore, these spectra revealed that males had fluorescent crowns with substantially higher reflectance than those of females, in both the UV waveband and at longer wavelengths. Reflectance spectrophotometry was also performed on a number of live wild-type male budgerigars to investigate the chromatic contrast between the different plumage areas. This showed that many plumage regions are highly UV-reflective. Overall our results suggest that rapid surveys using UVA black lamps may overestimate the contribution of fluorescence to plumage coloration, and that any signalling role of fluorescence emissions, at least from the yellow crown of budgerigars, may not be as important as previously thought.

Kaleidoscope configurations for reflectance measurement

Simulations of different configurations of the symmetrical tapered kaleidoscope are performed to assess their merits for measurement of BRDFs and BTFs. The relationship between optimal kaleidoscope layout, and factors such as hardware restrictions and the resolution of the required reflectance function, is derived. The effect on the measurement of the reflectance function of changing these independent variables is examined through the simulation. These experiments highlight issues affecting the measurement of BTFs using kaleidoscopes, and suggest configurations that allow sampling at regular parameter intervals. A number of other kaleidoscope architectures are explored, which offer the benefits of potentially doubling the range of directions that can be sampled, and allowing adaptive control of sample intervals.

Reflectance response of tapered optical fiber coated with graphene oxide nanostructured thin film for aqueous ethanol sensing

In this work, optical sensing performance of tapered multimode fiber tip coated with graphene oxide (GO) nanostructured thin film towards aqueous ethanol with different concentrations is investigated. The tapering process of the optical fiber is done by a glass processing machine. The multimode optical fiber tip is dip-coated with GO and annealed at 70 °C to enhance the binding of the nanomaterials to the silica fiber. FESEM, Raman microscopy and XRD analyses are performed to micro-characterize the GO thin films. The morphology of the GO is observed to be in sheets forms. The reflectance response of the GO coated fiber tip is compared with the uncoated tip. The measurements are taken using a spectrophotometer in the optical wavelength range of 550-720 nm. The reflectance response of the GO coated fiber tip reduced proportionally, upon exposure to ethanol with concentration range of 5-80%. The dynamic response of the developed sensor showed strong reversibility and repeatability when it is exposed to ethanol with concentrations of 5%, 20% and 40% in distilled water. At room temperature, the sensor shows fast response and recovery as low as 19 and 25 s, respectively. © 2014 Elsevier B.V.