Γεννημένος Δύο Φορές: η Dionysos Films και η Δημιουργία ενός Ελληνικού Κινηματογραφικού Κυκλώματος στην Αυστραλία

Από τα τέλη της δεκαετίας του ’40 μέχρι τα τέλη της δεκαετίας του ’70, το κέντρο και τα περίχωρα της Μελβούρνης στέγασαν ένα δυναμικό ελληνικό κινηματογραφικό κύκλωμα τριάντα περίπου διαφορετικών αιθουσών, οι οποίες λειτούργησαν υπό την εποπτεία ενός μικρού αριθμού καθετοποιημένων επιχειρήσεων προβολής/διανομής. Η Dionysos Films ήταν ανάμεσα στις πρώτες ελληνικές εταιρείες προβολής/διανομής που ιδρύθηκαν στην Αυστραλία και που από το 1949 ως το 1956 έδρασε χωρίς σημαντικό ανταγωνισμό, διαμορφώνοντας το πλαίσιο για ένα ελληνικό κινηματογραφικό κύκλωμα της διασποράς που εκτεινόταν από την επαρχιακή και μητροπολιτική Αυστραλία ως τη Νέα Ζηλανδία. Το παρόν άρθρο αναμετρά τη σκιά που έριξε η Dionysos Films (και ο χαρισματικός της ιδιοκτήτης Stathis Raftopoulos) στην ιστορία των κινηματογραφικών εμπειριών των Ελλήνων των Αντιπόδων καθώς και τις επιπτώσεις που έχει αυτή η ανεξερεύνητη πτυχή της αυστραλιανής, αλλά και της ελληνικής κινηματογραφικής ιστορίας, στον τρόπο που αντιλαμβανόμαστε τις εθνικές κινηματογραφίες των δύο χωρών.

Effect of Alpha-Linolenic acid on retnal function in mammals

The retinal function of several different species is compromised by a dietary deficiency of α-linolenic acid. A review of the literature revealed that diets containing less than O. 1 gIl OOg diet as α-linolenic acid could not sustain retinal docosahexaenoic acid levels over a prolonged period and that such diets were associated with a reduced response of the retina to light. In these studies the median α-linoleinic acid intake of control animals was 1.25g/100g diet. A study on the comparative ability of dietary α-linolenic acid and dietary docosahexaenoic acid to provide for retinal docosahexaenoic acid in the guinea pig found that α-linolenic acid was only approximately 10% as effective as docosahexaenoic acid in this regard.

Endurance training in humans leads to fiber type-specific increases in levels of peroxisome proliferator-activated receptor-[gamma] coactivator-1 and peroxisome proliferator-activated receptor-[alpha] in skeletal muscle

The peroxisome proliferator-activated receptor (PPAR)-γ coactivator-1 (PGC-1) can induce mitochondria biogenesis and has been implicated in the development of oxidative type I muscle fibers. The PPAR isoforms &alpha;, β/&delta;, and γ control the transcription of genes involved in fatty acid and glucose metabolism. As endurance training increases skeletal muscle mitochondria and type I fiber content and fatty acid oxidative capacity, our aim was to determine whether these increases could be mediated by possible effects on PGC-1 or PPAR-&alpha;, -β/&delta;, and -γ. Seven healthy men performed 6 weeks of endurance training and the expression levels of PGC-1 and PPAR-&alpha;, -β/&delta;, and -γ mRNA as well as the fiber type distribution of the PGC-1 and PPAR-&alpha; proteins were measured in biopsies from their vastus lateralis muscle. PGC-1 and PPAR-&alpha; mRNA expression increased by 2.7- and 2.2-fold (P < 0.01), respectively, after endurance training. PGC-1 expression was 2.2- and 6-fold greater in the type IIa than in the type I and IIx fibers, respectively. It increased by 2.8-fold in the type IIa fibers and by 1.5-fold in both the type I and IIx fibers after endurance training (P < 0.015). PPAR-&alpha; was 1.9-fold greater in type I than in the II fibers and increased by 3.0-fold and 1.5-fold in these respective fibers after endurance training (P < 0.001). The increases in PGC-1 and PPAR-&alpha; levels reported in this study may play an important role in the changes in muscle mitochondria content, oxidative phenotype, and sensitivity to insulin known to be induced by endurance training.

Revisiting delta-6 desaturate regulation by C18 unsaturated fatty acids, depending on the nutritional status

Dietary polyunsaturated fatty acids (PUFA) play a key role in regulating delta-6 desaturase (D6D), the key enzyme for long-chain PUFA biosynthesis. Nevertheless, the extent of their effects on this enzyme remains controversial and difficult to assess. It has been generally admitted that C18 unsaturated fatty acids (UFAs) regulate negatively delta-6 desaturase (D6D). This inhibition has been evidenced in regard to a high glucose/fat free (HG/FF) diet used in reference. However, several nutritional investigations did not evidence any inhibition of desaturases when feeding fatty acids.

Because the choice of the basal diet appeared to be of primary importance in such experiments, our goal was to reconsider the specific role of dietary UFAs on D6D regulation, depending on nutritional conditions. For that, sixteen adult Wistar rats were fed purified linoleic acid, &alpha;-linolenic acid or oleic acid, included in one of two diets at 4% by weight: an HG/FF or a high starch base (HS) where the pure UFAs replaced a mixed vegetable oil. Our results showed first that D6D specific activity was significantly greater when measured in presence of an HG/FF than with an HS/4% vegetable oil diet. Secondly, we found that linoleic and alpha-linolenic acids added to HG/FF reduced the specific activity of D6D. In contrast, when pure UFAs were added to an HS base, D6D specific activities remained unchanged or increased. Concordant results were obtained on D6D mRNA expression.

Altogether, this study evidenced the importance of the nutritional status in D6D regulation by C18 UFAs: when used as control, HG/FF diet stimulates D6D compared with a standard control diet containing starch and 4% fats, leading to an overestimation of the D6D regulation by UFAs. Then, UFAs should be considered as repressors for unsaturated fatty acid biosynthesis only in very specific nutritional conditions.

IEF pattern classification-derived criteria for the identification of epoetin-delta in urine

Epoetin-&delta; (Dynepo™ Shire Pharmaceuticals, Basing stoke, UK) is a synthetic form of erythropoietin (EPO) whose resemblance with endogenous EPO makes it hard to identify using the classical identification criteria. Urine samples collected from six healthy volunteers treated with epoetin-&delta; injections and from a control population were immuno-purified and analyzed with the usual IEF method. On the basis of the EPO profiles integration, a linear multivariate model was computed for discriminant analysis. For each sample, a pattern classification algorithm returned a bands distribution and intensity score (bands intensity score) saying how representative this sample is of one of the two classes, positive or negative. Effort profiles were also integrated in the model. The method yielded a good sensitivity versus specificity relation and was used to determine the detection window of the molecule following multiple injections. The bands intensity score, which can be generalized to epoetin-&alpha; and epoetin-β, is proposed as an alternative criterion and a supplementary evidence for the identification of EPO abuse.