Stepwise synthesis of Gly−Gly−His on gold surfaces modified with mixed self-assembled monolayers

Peptide-modified electrode surfaces have been shown to have excellent recognition properties for metal ions. An efficient method of screening a potential peptide for its selectivity for a given metal would involve the synthesis of the peptide directly on the electrode surface. This paper outlines a procedure in which the tripeptide Gly−Gly−His was synthesized one amino acid at a time on a gold surface modified with a self-assembled monolayer of the mixed alkanethiolates 3-mercaptopropionic acid (MPA) and 3-mercaptopropane (MP). Electrochemistry and high-resolution mass spectrometry were used to elucidate the structure of the adsorbed species and follow the synthesis. The amino acids can be attached only to MPA, but the presence of a diluting unreactive molecule of MP reduces steric crowding about the reaction center. The maximum coverage of synthesized tripeptide occurs at a ratio of MPA/MP of 1:1.

Plant pathogens causing vegetation dieback: a serious threat to the conservation of small mammals in Australia

The soil-borne plant pathogen Phytophthora cinnamomi occurs in most Australian states. It is pathogenic to many Australian species, particularly the Proteaceae, Fabaceae, Dillineaceae and Epacridaceae. In Western Australia, c. 2000 of the 9000 endemic plant species are directly affected by the disease. The epidemic of plant deaths caused by P. cinnamomi is recognised as one of 11 Key Threatening Processes to the Australian Environment, and is now also acknowledged as a potential threat fauna in a range of communities. The implications of landscape modification due to the effects of P. cinnamomi dieback prompted our research, designed to measure the distribution and abundance of small mammals in disease-affected ecosystems. This study was in the Jarrah (Eucalyptus marginata) forests in the Darling Range, Western Australia and measured the distribution and abundance of one small mammal species, the Mardo (Antechinus flavipes) by Elliott trapping in forests with (1) high, (2) mixed and (3) no evidence of Phytophthora dieback. Trap success was highest in sites with no effect of Phytophthora (7.3 animals per 100 trap nights), whereas the lowest trap success was recorded at the high impact sites (0.67 animals per 100 trap night). There was a significant difference in trap success of Mardos in Elliott trapping over 1800 trap nights (x²= 23.19, d.f = 5, p < 0.001). An examination of the distribution of individuals and sexes suggests that Phytophthora-affected sites act as sinks for Mardos, while source areas are healthy, unaffected Jarrah forest.

Manipulating Islam and democracy in Iraq’s ‘Pluralised Authoritarianism’

With the realisation that the initial motives for the 2003 invasion of Iraq – Saddam’s alleged stockpile of Weapons of Mass Destruction (WMD) and his links to Al-Qaeda – were grievous intelligence errors the Bush administration, with varying degrees of success, were able to spin the war’s rasion d’etre and redefine the parameters of victory. A central tenet of this approach was to begin speaking about democracy as if it had always been one of the aims of the war itself. For the first few years, the effort to democratise Iraq appeared to gain some credible momentum: a complex array of political, religious and ethno-sectarian factions formed political parties and civil society movements; uncensored news was enthusiastically consumed across the nation; Iraqi citizens took to the streets to protest key government decisions; and millions of Iraqis voted in relatively free and fair national elections (Davis, 2004, 2007, Isakhan, 2008, 2011b). Central to each of these developments were various Iraqi religious establishments – but especially those of the Shia Arab population of Iraq – who saw no distinction between their Islamic faith and the notion of democracy. Not surprisingly, a body of literature has emerged which has been very optimistic about Iraq’s engagement with both ‘Islam’ and ‘democracy’ in the post-Baathist period, while acknowledging the challenges it faces in creating a stable, egalitarian and democratic society (Al-Musawi, 2006, Cole, 2006, Davis, 2005, Dawisha, 2009, Isakhan, 2011a, Stansfield, 2007).

However, there have been virtually no studies which have sought to question this optimism in the light of more recent events. Addressing this lacuna, this paper documents the last few years (2006- 2011) which have seen many elements within the Iraqi political elite – most notably the Maliki government and his State of Law Coalition (SLC) – demonstrate what has been referred to in literature on other Arab states alternatively as ‘liberalised autocracy’ (Brumberg, 2002), ‘semi-authoritarianism’ (Ottaway, 2003) or ‘pluralised authoritarianism’ (Posusney and Angrist, 2005). That is to say, that these states consolidate their incumbency while putting in place measures that can be considered more or less liberal. To do this, the regime actually utilises (and controls) nominally democratic mechanisms such as elections, media freedoms, political opposition and civil society as part of their strategy to retain power. Of particular interest here are the ways in which the Maliki government – and Shia Arab Iraqi political factions more broadly – have manipulated both ‘Islam’ and ‘democracy’ towards such ‘pluralised authoritarianism’.

Academic governance in the contemporary university: perspectives from Anglophone nations

This book addresses three central questions in contemporary university governance: (1) How and why has academic governance in Anglophone nations changed in recent years and what impact have these changes had on current practices? (2) How do power relations within universities affect decisions about teaching and research and what are the implications for academic voices? (3) How can those involved in university governance and management improve academic governance processes and outcomes and why is it important that they do so? The book explores these issues in clear, concise and accessible language that will appeal to higher education researchers and governance practitioners alike. It draws on extensive empirical data from key national systems in the Anglophone world but goes beyond the simply descriptive to analyse and explain.

Influence of stocking rate and mixed grazing of Angora goats and Merino sheep on animal and pasture production in southern Australia : 3 : mohair and wool production and quality

The effects of animal species (AS; Angora goats, Merino sheep, mixed-grazed goats and sheep at the ratio of 1:1) and stocking rate (SR; 7.5, 10 and 12.5 animals/ha) on fibre production and quality were determined in a replicated experiment on improved annual temperate pastures in southern Australia from 1981 to 1984. Separately grazed sheep produced the most total clean fibre/ha at each SR. Mixed-grazed treatments produced amounts of clean fibre/ha similar to the arithmetic mean of sheep and goat treatments at 7.5/ha (21.9 versus 21.3 kg/ha), 10% more at 10/ha (28.3 versus 25.3 kg/ha, P < 0.05) and 7% more at 12.5/ha (31.6 versus 29.6 kg/ha, P < 0.10). Clean wool production/head was affected by AS and SR but not year. Clean mohair production was affected by SR and year but not AS. Variation in mean fibre diameter (MFD) accounted for 67 and 71%, respectively, of the variation in clean wool and clean mohair production/head. There was an AS SR interaction for clean fibre production/t pasture. Growth rate of mohair was highest in autumn and least in summer. In each season, an increase in the SR reduced the clean mohair growth rate. Growth rate of wool was highest in spring and least in summer. Wool and mohair MFD were affected by an AS SR interaction. Mohair MFD was also affected by year and season. At 10/ha, wool from mixed-grazed sheep had a greater MFD than wool from separately grazed sheep (20.2 versus 18.9 μm) and mixed-grazed goats grew mohair 1 μm coarser than separately grazed goats. At 12.5/ha mixed-grazed goats grew mohair 1.9 μm finer than separately grazed goats. Mohair MFD was predicted by a multiple regression that included average liveweight for the period of fleece growth, season of growth (summer 1 μm finer than winter) and year (range 1.27 μm). Mohair MFD increased 4.7 μm/10 kg increase in average fleece-free liveweight (P = 6.4 10-14). Fleece-free liveweight alone accounted for 76.4% of the variation in mohair MFD. There was an AS SR interaction for the incidence of kemp and medullated fibres; under severe grazing pressure their incidence was suppressed. This experiment indicated that the principles associated with the effects of SR on wool production on annual temperate pastures apply to mohair production. Mixed grazing of Merino sheep and Angora goats produced complementary and competitive effects depending on the SR. Angora goats should not be grazed alone or mixed-grazed with sheep on annual temperate pastures at SR greater than that recommended for Merino sheep.

Body image ideal among males and females: sociocultural influences and focus on different body parts

Past research has obtained mixed findings in relation to gender differences and other factors that shape ideal body image. The current study was designed to investigate these differences further, using a digital body image program to assess body image individually. As expected, females desired a body that was smaller than their current size, whereas males were split between wanting a smaller and larger body. The perceived messages from peers and parents were consistent with the individual's ideal image. Gender differences were also found with ratings of attractiveness and effectiveness. Females rated messages from peers and parents as more important than did males. For both males and females, opposite sex peers were the most important influences, and parents the least important.

Mixed cultures of food-grade probiotic bacteria and enteric bacteria demonstrate both synergism and inhibition on menaquinone production

The interaction between probiotic (Enterococcus spp., Lactobacillus spp., and Lactococcus spp.) and enteric (Bacteroides spp., Escherichia coli, and Salmonella spp.) bacteria with respect to menaquinone production was examined. Menaquinones were measured in cell pellets by high-pressure liquid chromatography and the main homologues produced were MIK_7–11. The growth of both Bacteroides and E. coli cultured with the 3 probiotics was significantly inhibited with concomitant reduction in menaquinone production. The vitamin K status of humans could be affected by consumption of probiotic dairy foods via the contribution made by gut microflora.

The first spacer-bridged tetraorganodistannoxanes with a mixed double ladder structure

The reaction of Me₃SiCH₂Cl₂Sn(CH₂)₃SnCl₂Ph (6) with (tBu₂SnO)₃ gave a statistical mixture of the corresponding tetraorganodistannoxanes whereas the reaction of the spacer-bridged ditin tetrachlorides RCl₂Sn(CH₂)₄SnCl₂R (3, R = Me₃CCH₂; 4, R = Me₂CHCH₂; 10, R = Me₃SiCH₂) with the polymeric spacer-bridged ditin oxides [R(O)Sn(CH₂)₄Sn(O)R]_n (7, R = Me₂CHCH₂; 8, R = Me₃CCH₂; 11, R = Me₃SiCH₂) provided the mixed double ladder compounds {[R(Cl)Sn(CH₂)₄Sn(Cl)R][R(Cl)Sn(CH₂)₄Sn(Cl)R']O₂}₂ (9, R = Me₃CCH₂, R' = Me₂CHCH₂; 12, R = Me₃CCH₂, R' = Me₃SiCH₂) in almost quantitative yield. In solution, 9 and 12 are in equilibrium with their corresponding dimers, as was evidenced by ¹¹⁹Sn NMR spectroscopy, molecular mass determination, and electrospray mass spectrometry. The molecular structures of 9 and 12 were established by single crystal X-ray diffraction.

A biogeographically mixed late Guadalupian (late Middle Permian) brachiopod fauna from an exotic limestone block at Xiukang in Lhaze county, Tibet

A total of 17 brachiopod species belonging to 15 genera are recorded from a limestone block of about 3×4 km2 in the Indus–Tsangbo suture zone at Xiukang in Lhaze County of Tibet. The brachiopod fauna generally indicates a Late Guadalupian age (late Wordian–Capitanian, late Middle Permian) based on its association with the Timorites-bearing ammonoid fauna and the presence of the brachiopod Urushtenoidea crenulata. Palaeobiogeographically, the fauna exhibits transitional/mixed characters between the warm-water Cathaysian and cold to temperate Gondwanan faunas and may have developed on a carbonate build-up or seamount on the oceanic crust.

Claiming the (n)either/(n)or of 'third space': (re)presenting hybrid identity and the embodiment of mixed race

As a multiracial artist, I am interested in how people of mixed race have been represented in popular culture and how mixed race image-makers can redress popular representation and facilitate a movement beyond the dichotomy, which seeks to reduce us to the sum of our parts. In the footsteps of Evelyn Alsultany I advocate the creation of a new cartography—a space that is inclusive and beyond existing notions of race. To this end I embarked on a project of exploration of the representation of multiracial identity, drawing from Homi K. Bhabha’s notion of Third Space.

No effect of n - 3 long-chain polyunsaturated fatty acid (EPA and DHA) supplementation on depressed mood and cognitive function : a randomised controlled trial

Low dietary intakes of the n-3 long-chain PUFA (LCPUFA) EPA and DHA are thought to be associated with increased risk for a variety of adverse  outcomes, including some psychiatric disorders. Evidence from  observational and intervention studies for a role of n-3 LCPUFA in depression is mixed, with some support for a benefit of EPA and/or DHA in major depressive illness. The present study was a double-blind randomised controlled trial that evaluated the effects of EPA+DHA supplementation (1.5 g/d) on mood and cognitive function in mild to moderately depressed  individuals. Of 218 participants who entered the trial, 190 completed the planned 12 weeks intervention. Compliance, confirmed by plasma fatty acid concentrations, was good, but there was no evidence of a difference between supplemented and placebo groups in the primary outcome - namely, the depression subscale of the Depression Anxiety and Stress Scales at 12 weeks. Mean depression score was 8.4 for the EPA+DHA group and 9.6 for the placebo group, with an adjusted difference of - 1.0 (95 % CI - 2.8, 0.8; P = 0.27). Other measures of mood, mental health and cognitive function, including Beck Depression Inventory score and attentional bias toward threat words, were similarly little affected by the intervention. In conclusion, substantially increasing EPA+DHA intake for 3 months was found not to have beneficial or harmful effects on mood in mild to moderate depression. Adding the present result to a meta-analysis of previous relevant randomised controlled trial results confirmed an overall negligible benefit of n-3 LCPUFA supplementation for depressed mood.

Estimation of in vitro prebiotic activity of individual and mixed dietary fibres

Background: Epidemiological studies have demonstrated a link between dietary fibre deficiency and prevalence of many “Western diseases” particularly colon related diseases. Many of the health benefits associated with dietary fibre are attributed to their prebiotic effect. However, not all fibres have the same prebiotic potential or the same impact on colon health.

Objective: To examine the in vitro fermentation properties of individual and mixed dietary fibres by measuring fermentation byproducts over time.

Design: Wheat bran and guar gum were selected for this study. Individual and mixed dietary fibres were added to batch fermentation system and were inoculated with fresh faecal inoculum (n= 4). Positive (inulin) and negative (no substrate) fermenters were also used to determine the differences. The pH of the five fermenters was adjusted to a baseline of 5.5 and 6.8 representing the pH of the proximal and distal sections of the colon respectively. Samples were drawn out of the fermenters at 0, 3, 9 and 24 hours for the analysis of pH, ammonia and short chain fatty acids (SCFAs).

Outcomes: There were no significant differences in the pH levels at various time points between fermenters adjusted to pH 5.5 at baseline. However, in fermenters adjusted to pH 6.8 the pH of the fermenter containing wheat bran increased over the time (24h (P = 0.017)) due to production of a high amount of ammonia. The total SCFAs production was greater in fermenters containing combined fibres.

Conclusion: There is a large inter-individual variation in the prebiotic effect of all types of dietary fibres, however, in the present study, dietary fibre combinations showed greater prebiotic potential compared with the individual fibres.

Technology adoption : voices of teaching academics, educational designers and students

This folio presents three studies (a dissertation and two electives) which use qualitative case study methodologies to investigate technology adoption from three perspectives. Central to all three studies is the study context of Monash University. The Dissertation explores adoption of web-based learning and teaching approaches from the perspective of teaching academics as they incorporate these to facilitate their students’ learning. The study investigates teaching academics’ reasons for adopting these new technologies, the factors that influenced their adoption decisions, and the challenges they were confronted with, including the contributing factors that impacted on their adoption decisions. The study shows that while contextual factors such as power and politics of the school, department, faculty and the institution impact on adoption, supportive organisational infrastructures and policy frameworks are necessary to encourage adoption, including wider adoption. In turn, on going staff development, adoption of new work practices and being adaptive to changing work environments are key demands made on teaching academics as a result of adopting web-based teaching approaches. Elective 1, a smaller study, leads on from the dissertation and examines the impact of technology adoption on the evolving role of educational designers. The study identifies the educational designers’ role change in assisting teaching academics to move from more conventional forms of teaching to more technology based learner-centred collaborative models. An important aspect of the study is the managers’ perspectives of this role in a university that has adopted a strong flexible learning and technology policy. The findings show that educational designers now work as project managers in larger teams consisting of a wider range of professionals, their expanded role in introducing technology into learning designs, providing staff development in the area, and giving technical help including advice on copyright and intellectual property issues. Elective 2 explores student readiness to adopt these technologies for learning. The study is designed to achieve an understanding of three broad categories of learners from a first year design unit: (1) South East Asian and East Asian students, (2) all other international students, and (3) local Australian students are studied to examine their readiness for modes of learning that are flexible; their approaches to study in a creative discipline area; and their openness to using technology. Findings of the study are discussed under the key themes – dependence on the teacher and classroom environment, flexible learning and working alone, structure, communication and work patterns. The study concludes by discussing the possible cultural attributes that have an impact on the learning. The three studies found that the institution, its people, structures and processes must all adapt, evolve and grow in order to provide effective, engaging, student-centred web-based learning environments. Students in turn must be enabled to manage their study, make use of the technologies and maximise their learning experience. The findings revealed the stage of technology use reached at Monash University at the time of the study through the voices of the teaching academics, educational designers and students.

Influence of praseodymium - synergistic corrosion inhibition in mixed rare-earth diphenyl phosphate systems

Mixed rare-earth organophosphates have been investigated as potential corrosion inhibitors for AA2024-T3, and previously have shown synergistic inhibition behavior; however, the mechanism was not identified. In this paper, a key factor contributing to corrosion inhibition of AA2024-T3 with mischmetal diphenyl phosphate [Mm(dpp)3] is the unique stability of Pr(dpp)3 compared to other key rare earths in mischmetal. Although increasing pH causes precipitation of other components, the Pr compound is stable at higher pH. Electrochemically, a synergy is evident when Ce(dpp)3 and Pr(dpp)3 are combined. Raman mapping indicates the Pr(dpp)3 inhibitor leads to a more uniform coverage of the alloy.